RESUMO
The atria are anatomically complex three-dimensional (3-D) structures. Impulse propagation is dynamic and complex during both normal conduction and arrhythmia. Atria activation has traditionally been represented on two-dimensional surface maps, which have inherent inaccuracies and are difficult to interpret. Interactive computerized 3-D display facilitates interpretation of complex atrial activation sequence data obtained from form-fitting multipoint electrodes. Accordingly, the purpose of this article is to describe the application of 3-D form-fitting electrode molds to the 3-D mapping and display system developed in this laboratory for the study of complex cardiac arrhythmias. Computer generated 3-D surface models are created from a database of serial cross-sectional anatomical images. Points chosen on endocardial and epicardial surfaces in each cross-sectional image are processed to create polygons defining myocardial wall boundaries. The polygons from adjacent serial images are then combined, to create a 3-D surface model. The discrete anatomical locations of unit electrodes on multipoint electrode templates are then assigned in the proper position on the surface model. Computer analysis of simultaneous activation data from each unit electrode is performed based on parameters set by the user. Activation data from each unit electrode site are displayed on the computer surface model in a color spectrum correlating with a user-defined time scale. Activation sequence maps can be visualized as static isochrone maps, interval maps, or as dynamic maps at variable speeds, from any 3-D perspective. Thus, an interactive computerized 3-D display system is described, which allows anatomically superior analysis and interpretation of complex atrial arrhythmias.
Assuntos
Arritmias Cardíacas/fisiopatologia , Átrios do Coração/fisiopatologia , Sistema de Condução Cardíaco/fisiopatologia , Modelos Cardiovasculares , Arritmias Cardíacas/diagnóstico , Função Atrial/fisiologia , Gráficos por Computador , Eletrodos Implantados , Eletrofisiologia , Humanos , Processamento de Imagem Assistida por Computador , Processamento de Sinais Assistido por Computador , SoftwareRESUMO
The site of earliest extracellular electrical activation in the sinoatrial node (SAN) is known to shift in response to autonomic stimuli, but the mechanisms underlying this phenomenon and the determinants of the location of dominant pacemaker activity have not been elucidated. The present study was designed to characterize the spatial distribution of muscarinic cholinergic and beta-adrenergic receptors in the canine SAN and to determine whether a consistent relationship exists between autonomic receptor densities and the site of dominant pacemaker activity. We used quantitative light-microscopic autoradiography of radioligand binding sites to characterize the spatial distribution of muscarinic cholinergic and beta-adrenergic receptor subtypes in tissue sections containing the SAN and adjacent right atrial muscle from 18 canine hearts. Muscarinic receptor density was 5.4 times greater in SAN cells than in atrial myocytes (P < .01). Total beta-adrenergic receptor density was more than 3 times greater in SAN cells than in atrial myocytes (P < .0001), due entirely to the significantly greater number of beta 1-adrenergic receptors in the SAN. The region of dominant pacemaker activity, localized in 4 hearts with in vitro mapping, consistently exhibited greater densities of muscarinic and beta 1-adrenergic receptors than other SAN regions. Muscarinic receptor density in the dominant pacemaker region was 18 +/- 2% and 29 +/- 7% higher than in adjacent superior and inferior regions, respectively. beta 1-Receptor density in the dominant site was 53 +/- 5% and 26 +/- 4% higher than in adjacent superior and inferior SAN regions, respectively. Thus, the SAN is richly endowed with both muscarinic cholinergic and beta 1-adrenergic receptors.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Receptores Adrenérgicos beta/análise , Receptores Muscarínicos/análise , Nó Sinoatrial/química , Nó Sinoatrial/fisiologia , Animais , Autorradiografia , Interpretação Estatística de Dados , Cães , Eletrofisiologia , Átrios do Coração/química , Técnicas Histológicas , Técnicas In VitroRESUMO
Activation sequence maps derived during normal sinus rhythm from extracellular potentials in the canine right atrium exhibit widely separated sites of origin. The objectives of this study were to characterize the distribution of pacemakers within the right atrium and to determine the relationship of pacemaker action potentials to sites of earliest surface activation as well as to local extracellular electrograms. The right atria of six adult mongrel dogs were rapidly excised under deep pentobarbital sodium anesthesia and perfused with 95% O2-5% CO2 Krebs-Henseleit solution. Action potentials from the epicardial surface were recorded throughout the region bounded by the crista terminalis laterally and the atrial septum medially. Simultaneously, unipolar extracellular electrograms were recorded from 250 endocardial sites. The earliest pacemakers preceded the earliest electrogram by 63 +/- 34 ms; the latest pacemakers followed the earliest electrogram by 71 +/- 40 ms. Primary negativity in the extracellular electro gram did not predict the site of the earliest or dominant pace maker and in some cases was associated with the latest pace makers. We conclude that primary negativity and/or the sites of earliest activation reflect the point at which the impulse engages atrial myocardium, not the site of earliest pacemaker activity. As such, early extracellular activation appears to represent sites of exit from a relatively insulated sinus node.
Assuntos
Sistema de Condução Cardíaco/fisiologia , Nó Sinoatrial/fisiologia , Animais , Cães , Eletrofisiologia , Sistema de Condução Cardíaco/anatomia & histologia , Técnicas In Vitro , Nó Sinoatrial/anatomia & histologiaRESUMO
BACKGROUND: Since the atria are thin-walled structures, most studies that have examined the spread of activation in the atria have assumed that they behave electrophysiologically as a two-dimensional surface. It was the objective of this study to determine whether or not this assumption is true by simultaneously mapping the epicardial and endocardial activation sequences in the right atrium. METHODS AND RESULTS: Identical precisely superpositioned epicardial and endocardial electrode templates with 250 unipolar electrodes each were used to map the isolated canine right atrium (n = 8) during continuous perfusion and superfusion with Krebs-Henseleit buffer. Data were recorded during control conditions (normal sinus rhythm), continuous pacing (S1S1 = 300 msec), and premature stimulation (S1S2 = effective refractory period + 5 msec). Pacing was performed at two sites, one located on the inferior crista terminalis and one lateral to the crista terminalis on a pectinate muscle. Tachyarrhythmias were induced by a single extrastimulus during the continuous perfusion of acetylcholine (10(-3.5) mol/L). Individual electrode sites were correlated with the gross anatomy and histology. Activation time differences were calculated between each two corresponding epicardial and endocardial sites. There were differences in the activation times between the epicardium and endocardium during all experimental conditions. However, the average difference for each condition was < 1 msec, suggesting that overall activation did not spread faster on either the epicardium or the endocardium, even though in certain regions one surface could lead the other. The dispersion of time differences was smallest during normal sinus rhythm and continuous pacing (SD = 5.6-5.8 msec) and largest after premature stimulation (SD = 6.3 msec for crista pacing, p < 0.05; SD = 8.1 msec for pacing lateral to the crista, p < 0.001). Differences in the activation sequence correlated with the underlying anatomic architecture. The largest differences in activation times between the epicardium and endocardium were associated with those regions of the atrium where pectinate muscles ran below the epicardial surface. The pectinate muscles in those areas were often discontinuous with the epicardial surface and facilitated the discordant epicardial-endocardial activation. The discordant activation was also found in regions where the atrial wall thickness was < 0.5 mm and correlated with transmural differences in fiber orientation. A tachyarrhythmia induced in the presence of acetylcholine, which demonstrated a focal activation pattern, was shown to have a reentrant loop that used free-running muscle bundles connecting the epicardial and endocardial surfaces, resulting in a three-dimensional pathway. CONCLUSIONS: The findings of this study demonstrate that epicardial and endocardial activation can be discordant in specific regions and that discordance increases with abnormal activation sequences. Many of the differences in the epicardial and endocardial activation can be correlated with the heterogeneity of the anatomic architecture of the right atrium. The study also demonstrates that reentry can occur in a three-dimensional plane using the epicardial and endocardial surfaces connected by transmural muscle fibers.
Assuntos
Arritmias Cardíacas/fisiopatologia , Função do Átrio Direito/fisiologia , Estimulação Cardíaca Artificial , Sistema de Condução Cardíaco/fisiologia , Animais , Cães , Eletrofisiologia , Endocárdio/fisiologia , Átrios do Coração/anatomia & histologia , Pericárdio/fisiologia , Processamento de Sinais Assistido por ComputadorRESUMO
The supraventricular arrhythmias of atrial fibrillation (AF), both chronic and paroxysmal, and atrial flutter (AFL) have been more difficult to study than most other clinical arrhythmias. Initial epicardial mapping studies at Washington University in canine models and in patients undergoing surgical ablation of other supraventricular arrhythmias demonstrated that AFL resulted from a macroreentrant circuit that was thought to occur only on the right side of the atrium with passive depolarization of the left atrial tissue. Atrial fibrillation was initially demonstrated to be considerably more complex with multiple circuits present. Furthermore, these circuits occurred simultaneously on both the right and left atria. Inability to map the atrial septum and the orifices of the pulmonary veins, however, led to the development of second-generation form-fitting experimental endocardial templates for the canine studies and an endocardial right atrial template for the patient studies. These second-generation experimental maps demonstrated that AFL circuits could involve the fixed anatomic obstacles of the right and left atria and adjacent areas of conduction block, frequently involving the septal and pulmonary vein tissue, with passive depolarization of the contralateral atrium. In contrast to this single-circuit mechanism, AF was confirmed to result from varying degrees of multiple reentrant circuits, occurring transiently in time and migrating over the surface of both atria. Furthermore, the single clinical arrhythmia of AF could result from a spectrum of endocardially or epicardially mapped arrhythmias, ranging from rapid AFL with variable atrioventricular block on one end to very fine multiple-circuit AF on the other end. It was clear that the development of a surgical procedure to ablate AF would need to isolate the atrial tissue in such a way that the transient reentrant circuits responsible for AF could not form because they were extinguished by a fixed or surgically created (eg, a suture line) anatomic obstacle.(ABSTRACT TRUNCATED AT 250 WORDS)