RESUMO
Equid Gamma herpesvirus (eGHV) infections have been reported worldwide and may be correlated with clinical signs, e.g., affecting the respiratory tract in young horses. eGHV are shed by healthy horses as well as horses with respiratory tract disease. The prevalence in healthy Swiss horses is unknown to date but this data would provide valuable information for causal diagnosis in clinical cases and formulation of biosecurity recommendations. Nasal swabs from 68 healthy horses from 12 Swiss stables and 2 stables near the Swiss border region in Germany were analyzed by panherpes nested PCR. Positive samples were sequenced. A multivariable model was used to determine if sex, age, breed, canton, or stable had a significant effect on the shedding status of each detected eGHV. Overall, the eGHV prevalence was 59% (n = 68); the prevalence for equid herpesvirus-2 (EHV-2), equid herpesvirus-5 (EHV-5) and asinine herpesvirus-5 (AHV-5) was 38%, 12% and 9%, respectively. Co-infections with multiple eGHVs were observed in 25% of the positive samples. The odds of shedding EHV-2 decreased with age (p = 0.01) whereas the odds of shedding AHV-5 increased with age (p = 0.04). Breed, sex, canton, or stable had no significant association with eGHV shedding. As EHV-2 shedding was common in healthy horses a positive PCR result must be interpreted with caution regarding the formulation of biosecurity recommendations and causal diagnosis. As EHV-5 and AHV-5 shedding was less common than EHV-2, a positive test result is more likely to be of clinical relevance. Shedding of multiple eGHV complicates the interpretation of positive test results in a horse.
Assuntos
Herpesvirus Equídeo 1/isolamento & purificação , Nariz/virologia , Doenças Respiratórias/veterinária , Eliminação de Partículas Virais , Animais , Anticorpos Antivirais/sangue , DNA Viral/genética , Feminino , Gammaherpesvirinae/isolamento & purificação , Gammaherpesvirinae/fisiologia , Alemanha/epidemiologia , Herpesvirus Equídeo 1/classificação , Herpesvirus Equídeo 1/genética , Herpesvirus Equídeo 1/fisiologia , Doenças dos Cavalos/epidemiologia , Doenças dos Cavalos/virologia , Cavalos , Masculino , Doenças Respiratórias/epidemiologia , Doenças Respiratórias/virologia , Suíça/epidemiologia , ViremiaRESUMO
Many recent disease outbreaks in humans had a zoonotic virus etiology. Bats in particular have been recognized as reservoirs to a large variety of viruses with the potential to cross-species transmission. In order to assess the risk of bats in Switzerland for such transmissions, we determined the virome of tissue and fecal samples of 14 native and 4 migrating bat species. In total, sequences belonging to 39 different virus families, 16 of which are known to infect vertebrates, were detected. Contigs of coronaviruses, adenoviruses, hepeviruses, rotaviruses A and H, and parvoviruses with potential zoonotic risk were characterized in more detail. Most interestingly, in a ground stool sample of a Vespertilio murinus colony an almost complete genome of a Middle East respiratory syndrome-related coronavirus (MERS-CoV) was detected by Next generation sequencing and confirmed by PCR. In conclusion, bats in Switzerland naturally harbour many different viruses. Metagenomic analyses of non-invasive samples like ground stool may support effective surveillance and early detection of viral zoonoses.
Assuntos
Quirópteros/virologia , Fezes/virologia , Metagenômica/métodos , Viroma/genética , Vírus/genética , Zoonoses/virologia , Adenoviridae/classificação , Adenoviridae/genética , Animais , Quirópteros/classificação , Reservatórios de Doenças/virologia , Variação Genética , Genoma Viral/genética , Hepevirus/classificação , Hepevirus/genética , Humanos , Coronavírus da Síndrome Respiratória do Oriente Médio/classificação , Coronavírus da Síndrome Respiratória do Oriente Médio/genética , Filogenia , Rotavirus/classificação , Rotavirus/genética , Análise de Sequência de DNA/métodos , Suíça , Vírus/classificaçãoRESUMO
We report here the full-length genome sequence of a rhabdovirus strain detected in a pool of 21 Culex pipiens and Culex torrentium mosquitos collected in southern Switzerland. The genome has a length of 11,914 nucleotides and encodes five major putative open reading frames.