RESUMO
The Sec23p-Sec24p complex is a component of coat protein II-coated vesicles involved in protein export from the endoplasmic reticulum. We previously identified a novel Sec23p-interacting protein, p125, which consists of 1000 amino acids and comprises a proline-rich region and a phospholipase A(1) homology region. p125, when ectopically expressed in cultured cells, localizes to endoplasmic reticulum-Golgi intermediate regions. In the present study we showed that expressed p125 principally colocalizes with p115 and GM130, both of which are involved in vesicle tethering to Golgi membranes. Next, we determined the functional regions of p125 by expressing a p125 series with deletions. The results showed that the proline-rich region (residues 135-259) is responsible for the binding to Sec23p. For the correct localization of p125, a region (residues 135-1000) comprising both the proline-rich and phospholipase A(1) homology regions was required.
Assuntos
Proteínas de Transporte/metabolismo , Animais , Autoantígenos , Proteínas de Transporte/química , Proteínas de Membrana/metabolismo , Fosfolipases A/metabolismo , Prolina/metabolismo , Ligação Proteica , Proteínas de Ligação a RNARESUMO
We examined the effects of short chain and long chain ceramides on the stability of the Golgi apparatus. Short chain ceramides, C(2)- and C(6)-ceramides, blocked brefeldin A-induced Golgi disassembly without affecting the rapid release of Golgi coat proteins, whereas they did not inhibit brefeldin A-induced tubulation of endosomes. Both short chain ceramides also retarded Golgi disassembly induced by nordihydroguaiaretic acid and nocodazole, suggesting that they stabilize the Golgi apparatus. In contrast to short chain ceramides, natural long chain ceramides, when incorporated into cells or formed within cells upon treatment with sphingomyelinase or metabolic inhibitors, enhanced brefeldin A-induced Golgi disassembly. These results suggest that sphingolipid metabolism is implicated in the stability of the Golgi apparatus.
Assuntos
Ceramidas/metabolismo , Complexo de Golgi/fisiologia , Esfingolipídeos/metabolismo , Esfingosina/análogos & derivados , Animais , Brefeldina A/farmacologia , Bovinos , Linhagem Celular , Ceramidas/farmacologia , Complexo I de Proteína do Envoltório/metabolismo , Endocanabinoides , Endossomos/metabolismo , Inibidores Enzimáticos/farmacologia , Etanolaminas/farmacologia , Complexo de Golgi/efeitos dos fármacos , Complexo de Golgi/metabolismo , Complexo de Golgi/ultraestrutura , Masoprocol/farmacologia , Microscopia Eletrônica , Morfolinas/farmacologia , Nocodazol/farmacologia , Ácidos Oleicos , Esfingolipídeos/biossíntese , Esfingomielina Fosfodiesterase/antagonistas & inibidores , Esfingosina/metabolismo , Esfingosina/farmacologia , Rede trans-Golgi/efeitos dos fármacos , Rede trans-Golgi/fisiologia , Rede trans-Golgi/ultraestruturaRESUMO
N-Ethylmaleimide-sensitive factor (NSF) is an ATPase involved in many membrane fusion events within the exocytic and endocytotic pathways. In the present study we showed that NSF is associated with the nuclear envelope. Golgi-associated NSF was released from membranes upon incubation with Mg(2+)-ATP, reflecting the disassembly of a complex consisting of NSF, soluble NSF attachment proteins (SNAPs), and SNAP receptors (SNAREs). In contrast nuclear envelope-associated NSF in interphase cells was not released by the same treatment. During mitosis, however, it was released from nuclear membranes by Mg(2+)-ATP. These results suggest that the binding mode of nuclear membrane-associated NSF changes during the cell cycle.
Assuntos
Proteínas de Transporte/metabolismo , Membrana Nuclear/metabolismo , Proteínas de Transporte Vesicular , Trifosfato de Adenosina/farmacologia , Animais , Proteínas de Transporte/análise , Ciclo Celular , Divisão Celular , Fracionamento Celular , Núcleo Celular/química , Núcleo Celular/metabolismo , Citoplasma/metabolismo , Immunoblotting , Interfase , Laminas , Manosidases/análise , Mitose/efeitos dos fármacos , Proteínas Sensíveis a N-Etilmaleimida , Membrana Nuclear/ultraestrutura , Proteínas Nucleares/metabolismo , Células PC12 , RatosRESUMO
Members of the syntaxin family are target-soluble N-ethylmaleimide-sensitive factor-attachment protein receptors involved in vesicle docking and/or fusion within the exocytic and endocytotic pathways. By using the yeast two-hybrid system, we have identified a novel member of the syntaxin family, syntaxin 18, that binds to alpha-soluble N-ethylmaleimide-sensitive factor-attachment protein. Subcellular fractionation and immunocytochemical analysis revealed that syntaxin 18 is principally located in the endoplasmic reticulum. We examined the effect of overexpression of FLAG-tagged syntaxin 18 and a mutant lacking the N-terminal 81 amino acid residues on protein transport and organelles in the early secretory pathway. Both expressed proteins localized to the endoplasmic reticulum, and the expressed FLAG-syntaxin 18 caused remarkable aggregation of endoplasmic reticulum membranes. Although expression of the FLAG-syntaxin 18 lacking the N-terminal region produced less effect on the morphology of the endoplasmic reticulum, dispersion of the endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi was elicited. Moreover, overexpression of the FLAG-syntaxin 18 mutant inhibited protein export from the endoplasmic reticulum. These results taken together suggest that syntaxin 18 functions in transport between the endoplasmic reticulum and Golgi.
Assuntos
Retículo Endoplasmático/metabolismo , Complexo de Golgi/metabolismo , Proteínas de Membrana/metabolismo , Proteínas de Transporte Vesicular , Sequência de Aminoácidos , Animais , Sequência de Bases , Transporte Biológico , Grânulos Citoplasmáticos/metabolismo , Células HeLa , Humanos , Fígado/metabolismo , Fígado/ultraestrutura , Proteínas de Membrana/genética , Dados de Sequência Molecular , Proteínas do Tecido Nervoso/metabolismo , Proteínas Qa-SNARE , Ratos , Ratos Wistar , Proteínas SNARE , Alinhamento de SequênciaRESUMO
Disassembly of the Golgi apparatus is elicited by the action of nordihydroguaiaretic acid (NDGA) and this disassembly is prevented by the activation of heterotrimeric G proteins. In the present study we showed that overexpression of Galpha(z) or Galpha(i2) significantly suppresses the disassembly of the Golgi apparatus induced by NDGA. Overexpression of Gbeta(1)gamma(2), on the other hand, had no effect on NDGA-induced Golgi disassembly. Galpha(z) neither blocked Golgi disassembly induced by brefeldin A or nocodazole, nor interfered with protein transport, suggesting its specificity on the action of NDGA. Our results suggest that the alpha subunits of heterotrimeric G proteins are responsible for the maintenance of the Golgi structure.
Assuntos
Subunidades alfa Gi-Go de Proteínas de Ligação ao GTP , Subunidades alfa de Proteínas de Ligação ao GTP , Complexo de Golgi/fisiologia , Proteínas Heterotriméricas de Ligação ao GTP/fisiologia , Proteínas Proto-Oncogênicas/fisiologia , Animais , Transporte Biológico , Brefeldina A/farmacologia , Linhagem Celular , Membrana Celular/metabolismo , Inibidores de Ciclo-Oxigenase/farmacologia , Retículo Endoplasmático/metabolismo , Subunidade alfa Gi2 de Proteína de Ligação ao GTP , Complexo de Golgi/efeitos dos fármacos , Proteínas Heterotriméricas de Ligação ao GTP/biossíntese , Proteínas Heterotriméricas de Ligação ao GTP/genética , Masoprocol/farmacologia , Nocodazol/farmacologia , Inibidores da Síntese de Proteínas/farmacologia , Proteínas Proto-Oncogênicas/biossíntese , Proteínas Proto-Oncogênicas/genéticaRESUMO
Fibroblast growth factor (FGF)-9 is a glycosylated neurotrophic polypeptide highly expressed in brain. The mechanism for its secretion from expressing cells is unclear, because its primary structure lacks a cleavable signal sequence. We, therefore, investigated the mechanism and structural requirements for secretion of FGF-9. As with other secreted proteins, in vitro translation of FGF-9 was inhibited by signal recognition particle, which binds to the signal sequence. When translated in vitro, full-length FGF-9 was translocated into microsomes, glycosylated, and protected from trypsin digestion. By using various FGF-9 deletion mutants, we found that two hydrophobic domains, located at the N terminus and at the center of the FGF-9 primary structure, were crucial for translocation. Examination of various point mutants revealed that local hydrophobicity of the central hydrophobic domain, but not the N terminus, was crucial for translocation. Analogous results were obtained with respect to FGF-9 secretion from transfectant cells. Upon deletion of the complete sequence preceding it, the previously uncleavable hydrophobic domain appeared to serve as a cleavable signal sequence. Our results suggest that nascent FGF-9 polypeptides translocate into endoplasmic reticulum without peptide cleavage via a co-translational pathway in which both the N terminus and the central hydrophobic domain are important; thereafter, FGF-9 is glycosylated and secreted.
Assuntos
Fatores de Crescimento de Fibroblastos , Substâncias de Crescimento/química , Sequência de Aminoácidos , Animais , Células COS , Primers do DNA , Fator 9 de Crescimento de Fibroblastos , Glicosilação , Substâncias de Crescimento/genética , Substâncias de Crescimento/metabolismo , Microssomos/metabolismo , Dados de Sequência Molecular , Mutação , Biossíntese de Proteínas , Sinais Direcionadores de Proteínas , Coelhos , Ratos , Reticulócitos , Partícula de Reconhecimento de Sinal/metabolismo , Transfecção , TriticumRESUMO
COPII-coated vesicles are involved in protein transport from the endoplasmic reticulum to the Golgi apparatus. COPII consists of three parts: Sar1p and the two protein complexes, Sec23p-Sec24p and Sec13p-Sec31p. Using a glutathione S-transferase fusion protein with mouse Sec23p, we identified a novel mammalian Sec23p-interacting protein, p125, which is clearly distinct from Sec24p. The N-terminal region of p125 is rich in proline residues, and the central and C-terminal regions exhibit significant homology to phospholipid-modifying proteins, especially phosphatidic acid preferring-phospholipase A1. We transiently expressed p125 and mouse Sec23p in mammalian cells and examined their interaction. The results showed that the N-terminal region of p125 is important for the interaction with Sec23p. We confirmed the interaction between the two proteins by a yeast two-hybrid assay. Overexpression of p125, like that of mammalian Sec23p, caused disorganization of the endoplasmic reticulum-Golgi intermediate compartment and Golgi apparatus, suggesting its role in the early secretory pathway.
Assuntos
Proteínas de Transporte/metabolismo , Fosfolipases A/metabolismo , Fosfolipídeos/química , Proteínas/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Northern Blotting , Proteínas de Transporte/química , Linhagem Celular , Chlorocebus aethiops , Clonagem Molecular , Primers do DNA , DNA Complementar , Complexo de Golgi/metabolismo , Humanos , Camundongos , Dados de Sequência Molecular , Ácidos Fosfatídicos/metabolismo , Ligação Proteica , Proteínas/genética , Proteínas de Ligação a RNA , Homologia de Sequência de Aminoácidos , Células Vero , Proteínas de Transporte VesicularRESUMO
The Sec23p-Sec24p complex is a component of COPII-coated vesicles that mediate protein transport from the endoplasmic reticulum in yeast. The mammalian hypothetical protein KIAA0079 (KIAA0079p) exhibits sequence similarity to yeast Sec24p. KIAA0079p was co-eluted with mammalian Sec23p on gel filtration. In vitro binding experiments revealed that the C-terminal region of KIAA0079p binds to the N-terminal region of mammalian Sec23p. Overexpression of KIAA0079p caused a defect in protein export from the endoplasmic reticulum. These results support the idea that KIAA0079p is a functional homologue of yeast Sec24p.
Assuntos
Proteínas Fúngicas/metabolismo , Proteínas de Membrana/metabolismo , Proteínas de Saccharomyces cerevisiae , Animais , Sítios de Ligação , Transporte Biológico Ativo , Linhagem Celular , Chlorocebus aethiops , Retículo Endoplasmático/metabolismo , Proteínas Fúngicas/química , Proteínas Fúngicas/genética , Humanos , Técnicas In Vitro , Proteínas de Membrana/química , Proteínas de Membrana/genética , Proteínas/química , Proteínas/isolamento & purificação , Proteínas/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Transfecção , Células Vero , Proteínas de Transporte VesicularRESUMO
The treatment of normal rat kidney cells with N-ethylmaleimide caused the release of beta-COP, a component of coatomer, from the Golgi apparatus without causing disassembly of the organelle. The release of beta-COP, which was not due to depolymerization of microtubules, was markedly blocked by the activation of GTP-binding proteins by aluminum fluoride or a nonhydrolyzable analogue of GTP. To determine which component is N-ethylmaleimide-sensitive, we reconstituted the recruitment of coatomer from the bovine brain cytosol onto the Golgi apparatus in digitonin-permeabilized cells. In cells treated with N-ethylmaleimide before permeabilization, beta-COP was still recruited onto the Golgi apparatus. In contrast, beta-COP was not recruited when N-ethylmaleimide-treated bovine brain cytosol was used. These results suggest that the N-ethylmaleimide-sensitive factor(s) are present in the cytosol. It is known that coatomer and ADP-ribosylation factor-1 (ARF1) are the only cytoplasmic proteins needed for the assembly of Golgi-derived coated vesicles. N-Ethylmaleimide treatment of a coatomer-rich fraction did not affect the binding of beta-COP to the Golgi apparatus, whereas the same treatment of an ARF-rich fraction abolished beta-COP binding. Similar results were obtained using purified recombinant ARF1. Concomitant with inactivation, 0.85 mol of N-ethylmaleimide was incorporated into 1 mol of ARF1. ARF1 contains only one cysteine residue (Cys-159), which is located near the base moiety of the bound guanine nucleotide.
Assuntos
Etilmaleimida/farmacologia , Proteínas de Ligação ao GTP/efeitos dos fármacos , Complexo de Golgi/metabolismo , Fator 1 de Ribosilação do ADP , Fatores de Ribosilação do ADP , Subunidades gama do Complexo de Proteínas Adaptadoras , Animais , Bovinos , Células Cultivadas , Proteína Coatomer , Cisteína , Citosol/química , Etilmaleimida/química , Proteínas de Ligação ao GTP/química , Proteínas de Ligação ao GTP/metabolismo , Complexo de Golgi/efeitos dos fármacos , Complexo de Golgi/ultraestrutura , Guanosina 5'-O-(3-Tiotrifosfato)/farmacologia , Membranas Intracelulares/metabolismo , Rim/citologia , Proteínas de Membrana/efeitos dos fármacos , Proteínas de Membrana/metabolismo , Proteínas Associadas aos Microtúbulos/metabolismo , Paclitaxel/farmacologia , RatosRESUMO
N-Ethylmaleimide-sensitive factor (NSF) is required for multiple pathways of vesicle-mediated protein transport. Microinjection of a monoclonal anti-NSF antibody almost completely blocked brefeldin A-promoted Golgi disassembly without affecting the rapid release of beta-COP, a subunit of the Golgi coat proteins (COPI), from the Golgi apparatus. Similar results were obtained using a dominant-negative NSF which is known to compete with endogenous NSF. The present results suggest that an NSF-mediated step is present in the brefeldin A-promoted disassembly of the Golgi apparatus.
Assuntos
Proteínas de Transporte/metabolismo , Ciclopentanos/farmacologia , Complexo de Golgi/metabolismo , Complexo de Golgi/ultraestrutura , Inibidores da Síntese de Proteínas/farmacologia , Proteínas de Transporte Vesicular , Animais , Antibacterianos/farmacologia , Brefeldina A , Células CHO , Cricetinae , Complexo de Golgi/efeitos dos fármacos , Macrolídeos , Microscopia Eletrônica , Proteínas Sensíveis a N-EtilmaleimidaRESUMO
Nordihydroguaiaretic acid (NDGA) caused disassembly of the Golgi apparatus of NRK cells in a dose-, time-, and energy-dependent manner but not in a microtubule-dependent manner. In contrast to brefeldin A, NDGA did not cause release of beta-COP, a component of Golgi-derived vesicles. However, NDGA-induced disassembly was blocked by AlF4-, an activator of the heterotrimeric but not the small GTP-binding proteins. In digitonin-permeabilized cells, guanosine 5'-3-O-(thio)triphosphate (GTPgammaS) as well as AlF4- blocked the NDGA-promoted disassembly of the Golgi apparatus, and Gbetagamma (betagamma subunits of heterotrimeric G proteins) reversed this effect. Our present results suggest the possible involvement of heterotrimeric G proteins in the organization of the Golgi apparatus.
Assuntos
Proteínas de Ligação ao GTP/química , Proteínas de Ligação ao GTP/metabolismo , Complexo de Golgi/fisiologia , Animais , Linhagem Celular , Permeabilidade da Membrana Celular , Toxina da Cólera/farmacologia , Proteína Coatomer , Cicloeximida/farmacologia , Digitonina , Complexo de Golgi/efeitos dos fármacos , Complexo de Golgi/ultraestrutura , Guanosina 5'-O-(3-Tiotrifosfato)/farmacologia , Rim , Substâncias Macromoleculares , Masoprocol/farmacologia , Proteínas de Membrana/análise , Proteínas de Membrana/metabolismo , Proteínas Associadas aos Microtúbulos/análise , Proteínas Associadas aos Microtúbulos/metabolismo , Microtúbulos/efeitos dos fármacos , Microtúbulos/fisiologia , Microtúbulos/ultraestrutura , Ratos , Fatores de Virulência de Bordetella/farmacologiaRESUMO
Newly recognized mammalian secretory proteins such as preprolactin are translocated across the endoplasmic reticulum (ER) in a signal recognition particle (SRP)-dependent manner. Recent studies revealed that there are two recognition steps for signal peptides during this translocation. The first step is recognition by SRP, which results in elongation arrest, and the second step is interaction between signal peptides and the translocation channel embedded in the ER membrane. To determine the roles of the hydrophobic region of signal peptides in the recognition by SRP and the membrane-embedded translocation machinery, we constructed chimeric proteins consisting of the mature region of preprolactin and signal peptides containing different numbers of leucine residues. The translocation of these chimeric proteins was completely dependent on SRP, and the efficiency increased as the number of leucine residues increased up to 10 and then decreased. Although the efficiency of elongation arrest also increased as the number of leucine residues increased up to 10, it only slightly decreased as the number increased up to 20. Similar results were obtained when the hydrophobic region was replaced by alternate leucine and alanine residues, except that the most efficient translocation occurred when the number was 14. Taken together, the present results suggests that the total hydrophobicity of the hydrophobic region of signal peptides is a determinant for recognition by both SRP and the membrane-embedded translocation machinery, although the specificities of the two signal recognition steps are slightly different from each other.
Assuntos
Retículo Endoplasmático/metabolismo , Membranas Intracelulares/metabolismo , Sinais Direcionadores de Proteínas/química , Partícula de Reconhecimento de Sinal/metabolismo , Sequência de Aminoácidos , Proteínas da Membrana Bacteriana Externa/genética , Proteínas da Membrana Bacteriana Externa/metabolismo , Sítios de Ligação , Transporte Biológico , Escherichia coli , Concentração de Íons de Hidrogênio , Dados de Sequência Molecular , Peptídeos/metabolismo , Prolactina/genética , Prolactina/metabolismo , Precursores de Proteínas/genética , Precursores de Proteínas/metabolismo , Sinais Direcionadores de Proteínas/metabolismo , Proteínas Recombinantes de Fusão/metabolismoRESUMO
We have recently presented some sequence rules that govern the endoproteolytic processing of precursor proteins within the constitutive secretory pathway [Watanabe, T. et al. (1992) J. Biol. Chem. 267, 8270-8274; Watanabe, T. et al. (1993) FEBS Lett. 320, 215-218]. This cleavage seems to be catalyzed by furin, a mammalian homologue of the yeast processing endoprotease Kex2. In this study, not only by coexpression of furin and substrate precursors in a furin-deficient cell line but also by in vitro experiments using purified recombinant furin and substrate precursors, we determined the substrate specificity of furin. The sequence requirements of furin determined by the coexpression and in vitro experiments were essentially the same, and were consistent with the sequence rules for precursor cleavage within the constitutive secretory pathway.
Assuntos
Precursores de Proteínas/metabolismo , Subtilisinas/metabolismo , Transfecção , Sequência de Aminoácidos , Animais , Células CHO , Neoplasias do Colo/genética , Cricetinae , Furina , Humanos , Dados de Sequência Molecular , Células Tumorais CultivadasRESUMO
Proteolytic cleavage (nicking) of diphtheria toxin (DT) in the 14-amino acid loop subtended by the disulfide bond between Cys186 and Cys201 is required for the cytotoxic action of DT. The loop includes the consensus motif for cleavage by a membrane-anchored protease, furin. We found that a soluble form of furin cleaves intact DT between Arg103 and Ser194 in vitro. LoVo cells, a human colon carcinoma cell line, do not produce functional furin. We show here that intact DT is not cleaved by LoVo cells. The cells are resistant to intact DT, although they are sensitive to DT nicked by furin before it is added to the medium. When intact DT is added to LoVo/Fur1 cells, a stable transfectant of LoVo cells expressing mouse furin, nicked DT associated with the cells is observed. LoVo/Fur1 cells are sensitive to both intact and nicked DT. These results indicate that furin is involved in the toxicity of intact DT. Bafilomycin A1, an inhibitor of intracellular vesicle acidification, did not inhibit cleavage of intact DT by LoVo/Fur1 or Vero cells, indicating that cleavage can proceed in a neutral environment. Inhibitors of endocytosis decreased DT cleavage but did not eliminate it. We also found a small amount of nicked DT in the culture medium. These results may indicate that intact DT is cleaved age by cell-associated furin on the cell surface as well as in endocytotic vesicles.
Assuntos
Toxina Diftérica/metabolismo , Subtilisinas/metabolismo , Animais , Furina , Humanos , Hidrólise , Células Tumorais Cultivadas , Células VeroRESUMO
Furin has been proposed to be the endoprotease responsible for precursor cleavage at Arg-X-Lys/Arg-Arg (RXK/RR) sites within the constitutive secretory pathway. However, there was a possibility that other protease(s) is involved in this cleavage. We here characterized furin in human colon carcinoma LoVo cells, since these cells lacked the endogenous processing activity toward RXK/RR sites and recovered the activity by transfection of furin cDNA. Furin cDNA cloned from LoVo cells had one nucleotide deletion in the region covering the homo B domain which is essential for the endoproteolytic activity. LoVo cells transfected with a furin construct with the mutation showed no activity. Based on these data, we conclude that furin is the endoprotease that is involved in the precursor cleavage at RXK/RR sites within the constitutive secretory pathway.
Assuntos
Processamento de Proteína Pós-Traducional , Subtilisinas/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Células CHO , Clonagem Molecular , Neoplasias do Colo , Cricetinae , Furina , Humanos , Camundongos , Dados de Sequência Molecular , Mutagênese , Oligodesoxirribonucleotídeos , Poli A/isolamento & purificação , Poli A/metabolismo , Reação em Cadeia da Polimerase , RNA/isolamento & purificação , RNA/metabolismo , RNA Mensageiro , Mapeamento por Restrição , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido Nucleico , Especificidade por Substrato , Subtilisinas/genética , Transfecção , Células Tumorais CultivadasRESUMO
The hepatocyte growth factor/scatter factor (HGF/SF) receptor consists of an alpha- and a beta-subunit, which are derived from a single-chain precursor by endoproteolytic processing. The precursor is not proteolytically processed in LoVo colon carcinoma cells. The uncleaved receptor immunopurified from the cells was cleaved in vitro by furin. Furthermore, the HGF/SF receptor was proteolytically processed in LoVo cells transfected with furin cDNA. These results indicate that furin is a processing endoprotease for the HGF/SF receptor. Tyrosine autophosphorylation of the uncleaved receptor was induced by HGF/SF, and the growth of the cells expressing the uncleaved receptor was stimulated by HGF/SF, indicating that the proteolytic processing of the receptor is not essential for the signal transduction of HGF/SF.
Assuntos
Proteínas Proto-Oncogênicas/metabolismo , Subtilisinas/farmacologia , Sequência de Aminoácidos , Animais , Northern Blotting , Células CHO , Cricetinae , Eletroforese em Gel de Poliacrilamida , Endopeptidases/metabolismo , Furina , Expressão Gênica , Fator de Crescimento de Hepatócito/metabolismo , Humanos , Dados de Sequência Molecular , Fosforilação , Proteínas Proto-Oncogênicas c-met , Transdução de Sinais , Subtilisinas/genética , Transfecção , Células Tumorais CultivadasRESUMO
We have recently purified and characterized a truncated soluble form of furin from which the predicted transmembrane domain and cytoplasmic tail were deleted (Hatsuzawa, K., Nagahama, M., Takahashi, S., Takada, K., Murakami, K., and Nakayama, K. (1992) J. Biol. Chem. 267, 16094-16099). Our results showed that furin resembles the yeast Kex2 protease with respect to both its enzymic properties and substrate specificity. Here we demonstrate that the soluble form of furin is capable of converting the precursors of albumin and the third component of complement (proalbumin and pro-C3, respectively) in vitro to mature proteins. Thus furin mimics the Ca(2+)-dependent proalbumin and pro-C3 convertases found in the Golgi membranes (Brennan, S. O., and Peach, R. J. (1988) FEBS Lett. 229, 167-170; Oda, K. (1992) J. Biol. Chem. 267, 17465-17471). Furthermore we show that the variant alpha 1-antitrypsin Pittsburgh, which is a specific inhibitor of the Golgi proalbumin convertase, inhibits not only the Golgi pro-C3 convertase, but also the soluble furin. These results suggest a role for furin in the cleavage of proproteins transported via the constitutive pathway.
Assuntos
Complemento C3/metabolismo , Pré-Albumina/metabolismo , Pró-Proteína Convertases , Precursores de Proteínas/metabolismo , Proteínas de Saccharomyces cerevisiae , Saccharomyces cerevisiae/enzimologia , Serina Endopeptidases/metabolismo , Subtilisinas/metabolismo , Animais , Células CHO , Linhagem Celular , Clonagem Molecular , Complemento C3/genética , Cricetinae , Furina , Complexo de Golgi/enzimologia , Humanos , Cinética , Fígado/enzimologia , Pré-Albumina/genética , Precursores de Proteínas/genética , Processamento de Proteína Pós-Traducional , Proteínas Tirosina Quinases/metabolismo , Ratos , Proteínas Recombinantes/metabolismo , Subtilisinas/isolamento & purificação , Transfecção , Células Tumorais CultivadasRESUMO
Furin, a mammalian homolog of the yeast Kex2 protease, is associated with Golgi membranes and is involved in cleavage of precursor proteins at sites marked by the Arg-X-Lys/Arg-Arg (RXK/RR) motif. We have recently shown that a furin mutant lacking the transmembrane domain can be secreted from cDNA-transfected cells with proteolytic activity for the fluorogenic peptide t-butoxycarbonyl-Arg-Val-Arg-Arg-4-methylcoumarin-7- amide. In this study, we purified and characterized the recombinant furin from the conditioned medium of these cells. Furin was purified as a mixture of 83- and 81-kDa forms and a 96-kDa form. The differences in molecular mass were not due to differences in molecular mass were not due to differences in glycosylation. Moreover, all forms had the same NH2-terminal sequence beginning at the residue after the Arg-Ala-Lys-Arg sequence. These data suggest that the three different forms may be produced by differential COOH-terminal processing of a furin molecule and that mature furin may be autocatalytically produced. Both enzyme preparations showed a pH optimum at 7.0, required Ca2+ for the activity, and showed essentially the same inhibitor profile. These properties resembled those of the Kex2 protease. Both preparations efficiently cleaved fluorogenic peptides with an RXK/RR sequence and moderately cleaved a peptide with an RXXR sequence, but did not cleave dibasic peptides. The sequence requirements determined in vitro were compatible with those determined by expression studies in cultured cells. These data unequivocally demonstrate that furin is an endogenous cellular protease responsible for cleavage of precursor proteins mainly at RXK/RR sites.
Assuntos
Pró-Proteína Convertases , Proteínas Tirosina Quinases/metabolismo , Proteínas de Saccharomyces cerevisiae , Serina Endopeptidases/metabolismo , Subtilisinas/metabolismo , Sequência de Aminoácidos , Animais , Sítios de Ligação , Western Blotting , Células CHO , Cromatografia por Troca Iônica , Cricetinae , Eletroforese em Gel de Poliacrilamida , Furina , Humanos , Cinética , Camundongos , Dados de Sequência Molecular , Peso Molecular , Plasmídeos , Homologia de Sequência do Ácido Nucleico , Especificidade por Substrato , Subtilisinas/genética , Subtilisinas/isolamento & purificação , TransfecçãoRESUMO
Many peptide hormones and neuropeptides are produced from larger, inactive precursors through endoproteolysis at sites usually marked by paired basic residues (primarily Lys-Arg and Arg-Arg), or occasionally by a monobasic residue (primarily Arg). Based upon data concerning processing of prorenin and its mutants around the native Lys-Arg cleavage site expressed in mouse pituitary AtT-20 cells, we present the following sequence rules that govern mono-arginyl cleavages: (a) a basic residue at the fourth (position -4) or the sixth (position -6) residue upstream of the cleavage site is required, (b) at position -4, Arg is more favorable than Lys, and (c) at position 1, a hydrophobic aliphatic residue is not suitable. These rules are compatible with those proposed by comparison of precursor sequences around mono-arginyl cleavage sites. We also provide evidence that precursor cleavages at mono-arginyl and dibasic sites can be catalyzed by the same Kex2-like processing endoprotease, PC1/PC3.
Assuntos
Arginina , Pró-Proteína Convertases , Precursores de Proteínas/metabolismo , Processamento de Proteína Pós-Traducional , Proteínas de Saccharomyces cerevisiae , Serina Endopeptidases/metabolismo , Subtilisinas , Sequência de Aminoácidos , Animais , Sequência de Bases , Sítios de Ligação , Linhagem Celular , Humanos , Camundongos , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Oligodesoxirribonucleotídeos , Plasmídeos , Precursores de Proteínas/genética , Homologia de Sequência do Ácido Nucleico , Especificidade por Substrato , TransfecçãoRESUMO
We have recently shown that furin, a mammalian homologue of the yeast precursor-processing endoprotease Kex2, is involved in precursor cleavage at sites marked by the Arg-X-Lys/Arg-Arg motif within the constitutive secretory pathway. In this study, we analyzed molecular and enzymatic properties of furin expressed in Chinese hamster ovary cells using gene transfer techniques. COOH-terminal truncation analyses indicate that the polypeptide region significantly conserved among the Kex2 family members is required for the endoprotease activity of furin, while the COOH-terminal unconserved region containing the Cys-rich domain and the transmembrane domain is dispensable. A mutant of furin truncated up to the transmembrane domain from the COOH-terminus was secreted into the culture medium as an active form. The sequence requirements for precursor cleavage of this truncated furin determined in vitro were similar to those of wild-type furin determined by expression studies in cultured cells. It had a strong resemblance to the Kex2 protease in the inhibitor profile and pH dependency. These observations support the notion that furin is the endogenous endoprotease involved in precursor cleavage at Arg-X-Lys/Arg-Arg sites.