Online short videos promoting public breast cancer literacy: a pretest-posttest control group trial on efficiency, attitude, and influencing factors.

Background: Short videos on social media are playing an increasingly important role in cancer health education today. It is important to explore how the actual communication effect of health videos and the knowledge absorption of users are influenced by different factors of the video creation process. Objective: The objective of our study is to access the factors influencing breast cancer health education through short videos on efficiency and quality. Methods: Three pairs of videos about breast health were created and participants completed questionnaires before and after watching the videos. A paired t-test was used to analyze within-group change scores. RM-ANOVA was used to assess the relationship between the pretest, posttest, and three variables. Results: Watching short videos can significantly increase viewers' knowledge of related health topics (p < 0.05). The viewers' concentration level while watching was significantly higher for the video with background music (BGM) than for the video without BGM (p = 0.006). The viewers' willingness to share was significantly higher for the video with a progress bar than for the video without a progress bar (p = 0.02). Using an interpreter wearing a doctor's uniform instead of casual wear and setting a progress bar can significantly improve the efficiency of knowledge absorption (p < 0.05). Conclusion: A uniformed interpreter, BGM and a progress bar are factors influencing the efficiency of short health videos. They can be applied in video making to explore better ways of promoting cancer health education in the new mobile Internet environment.

[Feasibility analysis of puncture robot technology for the application of acupuncture robot].

The difficulties such as how to accurately locate acupoints and safely insert needles are presented in acupuncture robot. The puncture robot with high technological similarity to acupuncture robot is getting mature, and a large number of human trials and animal experiments have been conducted for the development of puncture robot. Through comparing the similarities and differences between puncture robot and acupuncture robot in the aspects of through-skin puncture, needle insertion and needle removal, the valuable technology of puncture robot is analyzed for the development of acupuncture robot, and the crucial direction of technology migration is determined. ①Integrating the mechanical feedback and medical imaging technology and utilizing the multi-modal perception to achieve the safety of acupuncture operation. ②Emphasizing the integration of the existing designs of chest puncture robot to realize the acupuncture operation with inhalation and exhalation involved. ③Focusing on the development of relevant technology of automatic needle removal through conducting the actual scenario of treatment with acupuncture robot in patients under non-anaesthetic condition.

Multiple Copies of Mobile Tigecycline Resistance Efflux Pump Gene Cluster tmexC2D2.2-toprJ2 Identified in Chromosome of Aeromonas spp.

The appearance and prevalence of novel plasmid-encoded tigecycline resistance efflux pump gene clusters tmexC1D1-toprJ1 and tmexC2D2-toprJ2 in Enterobacteriaceae have raised a threat to public health. Here, another tigecycline resistance gene cluster, tmexC2D2.2-toprJ2, was identified in two Aeromonas isolates recovered from fish meat and vegetables. Cloning confirmed the expression of tmexC2D2.2-toprJ2 mediated the resistance to tigecycline and decreased susceptibility to tetracyclines and cephalosporins in both Escherichia coli and Aeromonas. In an Aeromonas veronii strain, four copies of tmexC2D2.2-toprJ2 were located on the chromosome. Further analysis revealed that tmexC2D2.2-toprJ2 has been detected in the chromosomes of A. veronii, Aeromonas hydrophila, and Aeromonas caviae with one to four copies due to the insertion of a potential integrative transferable unit. The occurrence of multiple copies of chromosomal tmexC2D2.2-toprJ2 may act as a sink for this tigecycline resistance gene cluster, which requires continuous monitoring. IMPORTANCE Tigecycline is regarded as one of the few effective drugs against multidrug-resistant bacterial infection. However, mobile tigecycline resistance efflux pump gene clusters such as tmexC1D1-toprJ1 and its variants have been identified in both animal- and human-origin Enterobacteriaceae. In this study, we first found another efflux pump gene cluster, tmexC2D2.2-toprJ2, in the Aeromonas chromosome. This gene cluster could mediate tigecycline resistance and decrease susceptibility to tetracyclines and cephalosporins in the Aeromonas host strain. Meanwhile, tmexC2D2.2-toprJ2 was detected with multiple copies in Aeromonas spp. This multidrug resistance efflux pump gene cluster with multiple copy numbers might stably exist in Aeromonas and serve as a reservoir for tmexCD2-toprJ2, facilitating its persistent presence and spread.

Rhizobacteria communities reshaped by red mud based passivators is vital for reducing soil Cd accumulation in edible amaranth.

Red mud (RM) was constantly reported to immobilize soil cadmium (Cd) and reduce Cd uptake by crops, but few studies investigated whether and how RM influenced rhizobacteria communities, which was a vital factor determining Cd bioavailability and plant growth. To address this concern, high-throughput sequencing and bioinformatics were used to analyze microbiological mechanisms underlying RM application reducing Cd accumulation in edible amaranth. Based on multiple statistical models (Detrended correspondence analysis, Bray-Curtis, weighted UniFrac, and Phylogenetic tree), this study found that RM reduced Cd content in plants not only through increasing rhizosphere soil pH, but by reshaping rhizobacteria communities. Special taxa (Alphaproteobacteria, Gammaproteobacteria, Actinobacteriota, and Gemmatimonadota) associated with growth promotion, anti-disease ability, and Cd resistance of plants preferentially colonized in the rhizosphere. Moreover, RM distinctly facilitated soil microbes' proliferation and microbial biofilm formation by up-regulating intracellular organic metabolism pathways and down-regulating cell motility metabolic pathways, and these microbial metabolites/microbial biofilm (e.g., organic acid, carbohydrates, proteins, S2-, and PO43-) and microbial cells immobilized rhizosphere soil Cd via the biosorption and chemical chelation. This study revealed an important role of reshaped rhizobacteria communities acting in reducing Cd content in plants after RM application.

In vitro fermentation of Gracilaria lemaneiformis sulfated polysaccharides and its agaro-oligosaccharides by human fecal inocula and its impact on microbiota.

The fermentation behaviour of sulfated polysaccharides (GLP) and their agaro-oligosaccharides (GLO) derived from Gracilaria lemaneiformis were examined. During in vitro fermentation, GLP and GLO increased the concentrations of short chain fatty acids (SCFAs) and modulated the composition and diversity of gut microorganisms compared with control groups. GLP increased the abundance of Bacteroidetes and decreased the abundance of Firmicutes, while GLO increased the abundance of Firmicutes and Actinobacteria. Moreover, the abundances of potential pathogenic bacteria were reduced. Molecular weight and intrinsic viscosity of GLP decreased significantly from 2.15 × 105 to 1.22 × 105 Da, 374.45-113.91 mL/g, respectively. Furthermore, GLP was degraded into smaller degree of polymerization of oligosaccharides, with no significant change observed in GLO. Overall, this study revealed GLP and GLO could be beneficial for gastrointestinal tract by producing SCFAs and modulating intestinal microbes, indicating GLP and GLO are potentially sources of prebiotics in functional foods.

Unraveling the Mechanisms of the Excited-State Intermolecular Proton Transfer (ESPT) for a D-π-A Molecular Architecture.

Precise revealing the mechanisms of excited-state intermolecular proton transfer (ESPT) and the corresponding geometrical relaxation upon photoexcitation and photoionization remains a formidable challenge. In this work, the compound (E)-4-(((4H-1,2,4-triazol-4-yl)imino)methyl)-2,6-dimethoxyphenol (TIMDP) adopting a D-π-A molecular architecture featuring a significant intramolecular charge transfer (ICT) effect has been designed. With the presence of perchloric acid (35 %), TIMDP can be dissolved through the formation of a HClO4 -H2 O-OH(TIMDP)-N(TIMDP) hydrogen-bonding bridge. At the ground state, the ICT effect is dominant, giving birth to crystals of TIMDP. Upon external stimuli (e.g., UV light irradiation, electro field), the excited state is achieved, which weakens the ICT effect, and significantly promotes the ESPT effect along the hydrogen-bonding bridge, resulting in crystals of [HTIMDP]+ ⋅[H2 O]⋅[ClO4 ]- . As a consequence, the mechanisms of the ESPT can be investigated, which distorted the D-π-A molecular architecture, tuned the emission color with the largest Stokes shift of 242 nm, and finally, high photoluminescence quantum yields (12 %) and long fluorescence lifetimes (8.6 µs) have achieved. These results not only provide new insight into ESPT mechanisms, but also open a new avenue for the design of efficient ESPT emitters.

Concise synthesis of 2-methoxyestradiol from 17ß-estradiol through the C(sp2)-H hydroxylation.

A four-step route for the synthesis of 2-methoxyestradiol (5) starting from 17ß-estradiol (1) has been achieved with a 51% overall yield. The key step was the ruthenium-catalyzed ortho-C(sp2)-H bond hydroxylation of aryl carbamates. Using dimethyl carbamate as the directing group, [RuCl2(p-cymene)]2 as the catalyst, PhI(OAc)2 as the oxidant and trifluoroacetate/trifluoroacetic anhydride (1:1) as the co-solvent, the hydroxyl group could be singly installed at the 2-position of 3-dimethylcarbamoyloxyestradiol (2) with 65% yield. Subsequent methylation of hydroxy and removal of dimethyl carbamate afforded 2-methoxyestradiol (5).

Porous High-Valence Metal-Organic Framework Featuring Open Coordination Sites for Effective Water Adsorption.

The design and preparation of a porous high-valence metal-organic framework (MOF) featuring open coordination sites are of utmost importance for the development of adsorbent materials. Here in this work, the three-dimensional (3D) high-valence MOF [Er(dcbp)3/2(DMF)(H2O)2]·2H2O (HV-MOF-1; H2dcbp = 4,4'-dicarboxy-2,2'-bipyridine, DMF = N,N-dimethylformamide), which possesses permanent porosity and two open coordination sites, has been prepared and characterized. In the 3D framework, the dcbp molecules display two different bridging styles, resulting in ordered diamondlike pores with bared carboxyl oxygen and pyridine nitrogen atoms on dcbp exposed directly to the pores, generating hydrophilic characteristics and high water affinity. In addition, the open coordination sites act as arms to fix the adsorbed water molecules, providing high water adsorption capacity (5.95 mmol g-1) and selectivity. More importantly, the activated HV-MOF-1 species shows an energy-saving step for recycling (operation under 120 °C), demonstrating promise as a candidate for an adsorbent material with considerable water adsorption-desorption cycles.

Ultralarge Dielectric Relaxation and Self-Recovery Triggered by Hydrogen-Bonded Polar Components.

Subtle integration of rotatable polar components into dielectric crystals can contribute significantly to adjustable switching temperatures ( Ts) and dielectric relaxation behaviors. Currently, one of the biggest challenges lies in the design of optimal polar components with moderate motion resistance in a crystalline system. In this work, we demonstrate that under refrigerator conditions, rotatable hydrogen-bonded one-dimensional (1D) cationic chains, {[C2H6N5]+} n (C2H6N5 = 3,5-diamino-1,2,4-triazolinium), and two-dimensional (2D) anionic layers, {[(H2O)2·SO4]2-} n, can be generated in an organic salt, 3 ([C2H6N5]2·[(H2O)2·SO4]). Compared with the nonhydrated precursor, 2 ([C2H7N5]·[SO4]), the rotation of these 1D and 2D ionic species triggers a reversible phase transition and dielectric switching in 3. In addition, the significantly sluggish rotation of the 1D cationic chains from parallel to unparallel stacking and the counter-clockwise rotation of the 2D anionic layers, compared with their reverse processes, induce a frequency-dependent dielectric response with a more highly adjustable heating Ts↑ than the cooling Ts↓. More importantly, 3 possesses excellent self-recovery ability attributed to the highly dynamic character of the hydrogen-bonded ionic species. The strategy here can provide a fairly good model for designing dielectric crystals with desired rotatable polar components.

Atomically Thin Two-Dimensional Nanosheets with Tunable Spin-Crossover Properties.

Combining the fascinating advantages of ultrathin two-dimensional (2D) nanosheets with the nanostructuration of spin-crossover (SCO) materials represents an attractive target of controlled fabrication of SCO nano-objects at the device level. Here, we demonstrate that through facile-operating ultrasonic force-assisted liquid exfoliation technology the three-dimensional (3D) van der Waals SCO bulk precursor {[Fe(1,3-bpp)2(NCS)2]2 (1, 1,3-bpp = 1,3-di(4-pyridyl)-propane)} can be exfoliated into single-layered 2D nanosheets (NS-1). As a consequence, the magnetism has been tuned from complete paramagnetic (bulk precursors) to SCO transition at around 250 K (2D nanosheets). In addition, the metal-to-ligand charge transition (MLCT), the intraligand π-π* transition and the color display also have been altered both in colloidal suspension and in the solid state. These dramatic changes of physical-chemical properties at different forms and states can be attributed to the efficient cooperativity derived from the interlayer van der Waals interactions within the curly or vertically stacked 2D building blocks.

Single-Layered Two-Dimensional Metal-Organic Framework Nanosheets as an in Situ Visual Test Paper for Solvents.

Through a facile-operating ultrasonic force-assisted liquid exfoliation technology, the single-layered two-dimensional (2D) [Co(CNS)2(pyz)2] n (pyz = pyrazine) nanosheets, with a thickness of sub-1.0 nm, have been prepared from the bulk precursors. The atomically thickness and the presence of abundant sulfur atoms with high electronegativity arrayed on the double surfaces of the sheets are making this kind of 2D MOF (metal-organic framework) nanosheets highly sensitive to intermolecular interactions. As a result, it can be well dispersed in all kinds of solvents to give a stable colloidal suspension that can be maintained for at least one month, accompanied by significant solvatochromic behavior and various optical properties, which thus have shown the potential to be practically applicated as in situ visual test paper for solvent identification and solvent polarity measurements. More importantly, combined with a smartphone, this kind of 2D-MOF nanosheets can be developed into in situ visual test paper to identify isomers and determine the polarity of mixed solvents quantitatively and qualitatively, suggesting the promising application of a portable, economical, and in situ visual test strategy in real world.

Bidirectional Photoswitching via Alternating NIR and UV Irradiation on a Core-Shell UCNP-SCO Nanosphere.

Bidirectional photoswitching of molecular materials under ambient condition is of significant importance. Herein, we present for the first time that a core-shell UCNP-SCO nanosphere (UCNP = upconversion nanophosphor, SCO = spin crossover), which was composed of a UCNP core (NaYF4: 20 mol % Yb3+, 1 mol % Er3+) and an SCO iron(II) shell ([Fe(H2Bpz)2(bipy-COOH)], H2Bpz = dihydrobis(1-pyrazolyl)borate, bipy-COOH = 4,4'-dicarboxy-2,2'-bipyridine), can be reversibly photoswitched between the high-spin and low-spin states at room temperature in the solid state, via alternating irradiation with near-infrared (λ = 980 nm) and ultraviolet (λ = 310 nm) light. What's more, this reversible spin-state switching was accompanied by a variation of fluorescent spectrum and dielectric constants. The strategy here, that is, integrating the SCO iron(II) complex into a UCNP-SCO nanosphere for molecular photoswitching, may open a new area in the development of photocontrolled molecular devices.

Thermal-Induced Dielectric Switching with 40K Wide Hysteresis Loop Near Room Temperature.

A thermal-induced dielectric switching has been realized in two ion-pair crystal [C2H6N5]+·[H2PO4]- (1, C2H6N5 = 3,5-diamino-1,2,4-triazolinium) through single-crystal-to-single-crystal phase transition (SCSC-PT). Upon cooling from room temperature, the 1D cation stripes that are composed of [C2H5N5]+ cations have undergone a 90° sharp rotation around the c axis, accompanied by the transition of crystal stacking from loose unparallel (dynamic state) to compression parallel (static state) and reorientation of dipoles on the [C2H5N5]+ cation, which thus resulted in high dielectric state to low dielectric state transformation. While on the warming run, the reverse process was rather sluggish, resulting in a reversible dielectric switching with ultralarge (about 40K wide) hysteresis loop near room temperature. It is thought that the large-sized polar cation stripes have a predominant influence on the switching properties of 1.

Binding CO2 from Air by a Bulky Organometallic Cation Containing Primary Amines.

The organometallic cation 1 (Fe(bipy-NH2)32+, bipy-NH2 = 4,4'-diamino-2,2'-bipyridine), which was constructed in situ in solution, can bind CO2 from air effectively with a stoichiometric ratio of 1:4 (1/CO2), through the formation of "H-bonded CO2" species: [CO2-OH-CO2]- and [CO2-CO2-OH]-. These two species, along with the captured individual CO2 molecules, connected 1 into a novel 3D (three-dimensional) architecture, that was crystal 1·2(OH-)·4(CO2). The adsorption isotherms, recycling investigations, and the heat capacity of 1 have been investigated; the results revealed that the organometallic cation 1 can be recycled at least 10 times for the real-world CO2 capture applications. The strategies presented here may provide new hints for the development of new alkanolamine-related absorbents or technologies for CO2 capture and sequestration.

Ghrelin inhibits 5-fluorouracil-induced apoptosis in colonic cancer cells.

BACKGROUND AND AIM: The aim of the present study was to investigate if ghrelin inhibits apoptosis in colonic cancer cells. METHODS: Cell viability in HT-29 cells was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Apoptosis was measured using 4',6-diamidino-2-phenylindole staining and flow cytometry. The protein expression of Bcl-2, Bax, and caspase-3 activation was examined using Western blotting. RESULTS: Ghrelin dose dependently decreased the growth inhibition of HT-29 cells induced by 5-fluorouracil (5-FU). Cells treated with 5-FU displayed chromatin condensation and nuclear fragmentation, which are typical changes of apoptosis. However, co-treatment with ghrelin reduced these changes. Flow cytometry after staining with Annexin V and propidium iodide showed that ghrelin decreased the apoptotic rate of HT-29 cells induced by 5-FU. Caspase-3 activation was significantly lower in the co-treated group than in the group treated with 5-FU alone. In addition, ghrelin reversed the 5-FU-induced Bcl-2/Bax protein ratio. CONCLUSION: Ghrelin inhibits 5-FU-induced apoptosis in colon cancer cells through the regulation of the Bcl-2/Bax protein ratio.