RESUMO
Unicellular organisms that live in marine environments must cope with considerable fluctuations in the availability of inorganic phosphate (Pi). Here, we investigated the extracellular Pi concentration-dependent expression, as well as the intracellular or extracellular localization, of phosphatases and phosphate transporters of the diatom Phaeodactylum tricornutum. We identified Pi-regulated plasma membrane-localized, ER-localized, and secreted phosphatases, in addition to plasma membrane-localized, vacuolar membrane-localized, and plastid-surrounding membrane-localized phosphate transporters that were also regulated in a Pi concentration-dependent manner. These studies not only add further knowledge to already existing transcriptomic data, but also highlight the capacity of the diatom to distribute Pi intracellularly and to mobilize Pi from extracellular and intracellular resources.
RESUMO
Dimeric disulfide-linked peptides are formed by the regioselective oxidative folding of thiol precursors containing the CX3CX2CX3C tetracysteine motif. Here, we investigate the general applicability of this peptide as a dimerization motif for different proteins. By recombinant DNA technology, the peptide CHWECRGCRLVC was loaded with proteins, and functional homodimers were obtained upon oxidative folding. Attached to the N-terminus of the dodecapeptide, the prokaryotic enzyme limonene epoxide hydrolase (LEH) completely forms a covalent antiparallel dimer. In a diatom expression system, the monoclonal antibody CL4 mAb is released in its functional form when its natural CPPC central parallel hinge is exchanged for the designed tetra-Cys hinge motif. To improve our understanding of the regioselectivity of tetra-disulfide formation, we provoked the formation of heterodimeric hinge peptides by mixing two different tetra-Cys peptides and characterizing the heterodimer by mass spectrometry and nuclear magnetic resonance spectroscopy.
Assuntos
Cisteína/análogos & derivados , Oligopeptídeos/química , Multimerização Proteica , Sequência de Aminoácidos , Anticorpos Monoclonais/química , Dissulfetos/química , Hidrolases/química , Modelos Moleculares , Oxirredução , Dobramento de Proteína , Proteínas Recombinantes/químicaRESUMO
Microalgae are unicellular eukaryotic organisms which represent an emerging alternative to other cell biofactories commonly used to produce monoclonal antibodies. Microalgae display several biotechnological advantages such as their rapid growth rate and their phototrophic lifestyle allowing low production costs as protein expression is solar-fueled. Recently, a fully assembled recombinant IgG antibody directed against Hepatitis B surface antigen is produced and secreted in the culture medium of the diatom Phaeodactylum tricornutum. A biochemical characterization of this recombinant antibody demonstrated that the Asn-297 is N-glycosylated by oligomannosides. In the immune system, antibodies interact with effector molecules and cells through their Fc part and the recognition of Fcγ receptors (FcγR) which are important for inducing phagocytosis of opsonized microbes. Interactions between IgG and FcγR are influenced by the N-glycan structures present on the Asn-297. In this study, the authors characterized the binding capacity of the anti-hepatitis B recombinant IgG produced in P. tricornutum to two human Fcγ receptors (FcγRI and IIIa) using a cellular binding assay and surface plasmon resonance (SPR). This allowed us to demonstrate that the alga-made antibody is able to bind FcγRI with a reduced affinity and engages FcyRIIIa with 3-times higher affinity compared to a control human IgG1.
Assuntos
Anticorpos Monoclonais/metabolismo , Diatomáceas/crescimento & desenvolvimento , Antígenos de Superfície da Hepatite B/imunologia , Receptores de IgG/metabolismo , Asparagina/química , Meios de Cultura/química , Diatomáceas/metabolismo , Glicosilação , Células HEK293 , Humanos , Oligossacarídeos/metabolismo , Proteínas Recombinantes/metabolismo , Células THP-1RESUMO
An apical plasma membrane domain enriched in the regulatory phospholipid phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] is critical for polar tip growth of pollen tubes. How the biosynthesis of PtdIns(4,5)P2 by phosphatidylinositol 4-phosphate 5-kinases (PI4P 5-kinases) is controlled by upstream signaling is currently unknown. The pollen-expressed PI4P 5-kinase PIP5K6 is required for clathrin-mediated endocytosis and polar tip growth in pollen tubes. Here, we identify PIP5K6 as a target of the pollen-expressed mitogen-activated protein kinase MPK6 and characterize the regulatory effects. Based on an untargeted mass spectrometry approach, phosphorylation of purified recombinant PIP5K6 by pollen tube extracts could be attributed to MPK6. Recombinant MPK6 phosphorylated residues T590 and T597 in the variable insert of the catalytic domain of PIP5K6, and this modification inhibited PIP5K6 activity in vitro. PIP5K6 interacted with MPK6 in yeast two-hybrid tests, immuno-pull-down assays, and by bimolecular fluorescence complementation at the apical plasma membrane of pollen tubes. In vivo, MPK6 expression resulted in reduced plasma membrane association of a fluorescent PtdIns(4,5)P2 reporter and decreased endocytosis without impairing membrane association of PIP5K6. Effects of PIP5K6 expression on pollen tube growth and cell morphology were attenuated by coexpression of MPK6 in a phosphosite-dependent manner. Our data indicate that MPK6 controls PtdIns(4,5)P2 production and membrane trafficking in pollen tubes, possibly contributing to directional growth.
Assuntos
Arabidopsis/enzimologia , Membrana Celular/enzimologia , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Nicotiana/enzimologia , Fosfatidilinositol 4,5-Difosfato/metabolismo , Tubo Polínico/enzimologia , Tubo Polínico/crescimento & desenvolvimento , Motivos de Aminoácidos , Sequência de Aminoácidos , Arabidopsis/citologia , Biocatálise , Endocitose , Corantes Fluorescentes/metabolismo , Proteínas Quinases Ativadas por Mitógeno/química , Modelos Biológicos , Fosforilação , Fosfotreonina/metabolismo , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Tubo Polínico/citologia , Ligação Proteica/efeitos dos fármacos , Inibidores de Proteínas Quinases/farmacologia , Proteínas Recombinantes , Nicotiana/citologiaRESUMO
BACKGROUND: The ideal protein expression system should provide recombinant proteins in high quality and quantity involving low production costs only. However, especially for complex therapeutic proteins like monoclonal antibodies many challenges remain to meet this goal and up to now production of monoclonal antibodies is very costly and delicate. Particularly, emerging disease outbreaks like Ebola virus in Western Africa in 2014-2016 make it necessary to reevaluate existing production platforms and develop robust and cheap alternatives that are easy to handle. RESULTS: In this study, we engineered the microalga Phaeodactylum tricornutum to produce monoclonal IgG antibodies against the nucleoprotein of Marburg virus, a close relative of Ebola virus causing severe hemorrhagic fever with high fatality rates in humans. Sequences for both chains of a mouse IgG antibody were retrieved from a murine hybridoma cell line and implemented in the microalgal system. Fully assembled antibodies were shown to be secreted by the alga and antibodies were proven to be functional in western blot, ELISA as well as IFA studies just like the original hybridoma produced IgG. Furthermore, synthetic variants with constant regions of a rabbit IgG and human IgG with optimized codon usage were produced and characterized. CONCLUSIONS: This study highlights the potential of microalgae as robust and low cost expression platform for monoclonal antibodies secreting IgG antibodies directly into the culture medium. Microalgae possess rapid growth rates, need basically only water, air and sunlight for cultivation and are very easy to handle.
Assuntos
Anticorpos Monoclonais/metabolismo , Diatomáceas/metabolismo , Marburgvirus/genética , Ribonucleoproteínas/imunologia , Proteínas Virais/imunologia , Animais , Anticorpos Monoclonais/genética , Anticorpos Monoclonais/imunologia , Linhagem Celular , Ensaio de Imunoadsorção Enzimática , Humanos , Hibridomas/citologia , Hibridomas/metabolismo , Fragmentos Fc das Imunoglobulinas/genética , Fragmentos Fc das Imunoglobulinas/imunologia , Fragmentos Fc das Imunoglobulinas/metabolismo , Camundongos , Microalgas/metabolismo , Microscopia de Fluorescência , Proteínas do Nucleocapsídeo , Coelhos , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologiaRESUMO
Microalgae have an enormous ecological relevance as they contribute significantly to global carbon fixation. But also for biotechnology microalgae became increasingly interesting during the last decades as many algae provide valuable natural products. Especially the high lipid content of some species currently attracts much attention in the biodiesel industry. A further application that emerged some years ago is the use of microalgae as expression platform for recombinant proteins. Several projects on the production of therapeutics, vaccines and feed supplements demonstrated the great potential of using microalgae as novel low-cost expression platform. This review provides an overview on the prospects and advantages of microalgal protein expression systems and gives an outlook on potential future applications.
Assuntos
Microalgas/metabolismo , Engenharia de Proteínas/métodos , Proteínas Recombinantes/biossíntese , Animais , Regulação da Expressão Gênica/efeitos da radiação , Vetores Genéticos , Humanos , Luz , Microalgas/genética , Microalgas/efeitos da radiação , Complexos Multiproteicos , Multimerização Proteica , Estrutura Quaternária de Proteína , Subunidades Proteicas , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Relação Estrutura-Atividade , Transcrição Gênica/efeitos da radiaçãoRESUMO
Monoclonal antibodies (mAbs) represent actually the major class of biopharmaceuticals. They are produced recombinantly using living cells as biofactories. Among the different expression systems currently available, microalgae represent an emerging alternative which displays several biotechnological advantages. Indeed, microalgae are classified as generally recognized as safe organisms and can be grown easily in bioreactors with high growth rates similarly to CHO cells. Moreover, microalgae exhibit a phototrophic lifestyle involving low production costs as protein expression is fueled by photosynthesis. However, questions remain to be solved before any industrial production of algae-made biopharmaceuticals. Among them, protein heterogeneity as well as protein post-translational modifications need to be evaluated. Especially, N-glycosylation acquired by the secreted recombinant proteins is of major concern since most of the biopharmaceuticals including mAbs are N-glycosylated and it is well recognized that glycosylation represent one of their critical quality attribute. In this paper, we assess the quality of the first recombinant algae-made mAbs produced in the diatom, Phaeodactylum tricornutum. We are focusing on the characterization of their C- and N-terminal extremities, their signal peptide cleavage and their post-translational modifications including N-glycosylation macro- and microheterogeneity. This study brings understanding on diatom cellular biology, especially secretion and intracellular trafficking of proteins. Overall, it reinforces the positioning of P. tricornutum as an emerging host for the production of biopharmaceuticals and prove that P. tricornutum is suitable for producing recombinant proteins bearing high mannose-type N-glycans.
Assuntos
Anticorpos Monoclonais/biossíntese , Biotecnologia/métodos , Diatomáceas/metabolismo , Anticorpos Anti-Hepatite B/biossíntese , Imunoglobulina G/biossíntese , Microalgas/metabolismo , Sequência de Aminoácidos , Reatores Biológicos , Estabilidade de Medicamentos , Retículo Endoplasmático/metabolismo , Glicosilação , Antígenos de Superfície da Hepatite B/imunologia , Humanos , Espectrometria de Massas , Dados de Sequência Molecular , N-Acetilglucosaminiltransferases/metabolismo , Processamento de Proteína Pós-Traducional , Sinais Direcionadores de Proteínas , Transporte Proteico , Proteólise , Proteínas Recombinantes de Fusão/biossínteseRESUMO
Many protists with high ecological and medical relevance harbor plastids surrounded by four membranes. Thus, nucleus-encoded proteins of these complex plastids have to traverse these barriers. Here we report on the identification of the protein translocators located in two of the plastid surrounding membranes and present recent findings on the mechanisms of protein import into the plastids of diatoms.
Assuntos
Compartimento Celular/fisiologia , Diatomáceas/metabolismo , Plastídeos/metabolismo , Transporte Proteico/fisiologia , Proteínas/metabolismo , Membrana Celular/metabolismo , Plastídeos/genéticaRESUMO
Many important algae groups like diatoms, dinoflagellates and 'kelp' but also apicomplexan parasites evolved in secondary endosymbiosis. Here, a eukaryote-eukaryote endosymbiosis created chimeric cells, in which a eukaryotic symbiont was reduced to a complex plastid. Although having lost nearly all of the eukaryotic compartments of the symbiont, a tiny lumen representing the remnant of the cytoplasm of the symbiont is still present in most of these organisms. This compartment, the periplastidal compartment, shows different degrees of reductions as in two algal groups the former nucleus is still present in a minimized form, called nucleomorph, whereas most others have lost the genetic system completely. Thus, the natural reduction of eukaryotic cytoplasms can be studied in terms of evolution and functionality, giving additionally advices for the design of synthetic minimized compartments.
Assuntos
Citoplasma/metabolismo , Eucariotos/metabolismo , Plastídeos/metabolismo , Transporte Biológico , Eucariotos/genética , Genoma/genética , Simbiose/fisiologiaRESUMO
Complex plastids evolved by secondary endosymbiosis and are, in contrast to primary plastids, surrounded by 3 or 4 envelope membranes. Recently, we provided evidence that in diatoms proteins exist that get N-glycosylated during transport across the outermost membrane of the complex plastid. This gives rise to unique questions on the transport mechanisms of these bulky proteins, which get transported across up to 3 further membranes into the plastid stroma. Here we discuss our results in an evolutionary context and speculate about the existence of plastidal glycoproteins in other organisms with complex plastids.
Assuntos
Glicoproteínas/metabolismo , Plastídeos/metabolismo , Diatomáceas/genética , Diatomáceas/metabolismo , Transporte Proteico/genética , Transporte Proteico/fisiologia , Simbiose/genética , Simbiose/fisiologiaRESUMO
Plastid and mitochondrial genomes have undergone parallel evolution to encode the same functional set of genes. These encode conserved protein components of the electron transport chain in their respective bioenergetic membranes and genes for the ribosomes that express them. This highly convergent aspect of organelle genome evolution is partly explained by the redox regulation hypothesis, which predicts a separate plastid or mitochondrial location for genes encoding bioenergetic membrane proteins of either photosynthesis or respiration. Here we show that convergence in organelle genome evolution is far stronger than previously recognized, because the same set of genes for ribosomal proteins is independently retained by both plastid and mitochondrial genomes. A hitherto unrecognized selective pressure retains genes for the same ribosomal proteins in both organelles. On the Escherichia coli ribosome assembly map, the retained proteins are implicated in 30S and 50S ribosomal subunit assembly and initial rRNA binding. We suggest that ribosomal assembly imposes functional constraints that govern the retention of ribosomal protein coding genes in organelles. These constraints are subordinate to redox regulation for electron transport chain components, which anchor the ribosome to the organelle genome in the first place. As organelle genomes undergo reduction, the rRNAs also become smaller. Below size thresholds of approximately 1,300 nucleotides (16S rRNA) and 2,100 nucleotides (26S rRNA), all ribosomal protein coding genes are lost from organelles, while electron transport chain components remain organelle encoded as long as the organelles use redox chemistry to generate a proton motive force.
Assuntos
Cianobactérias/genética , Genoma Mitocondrial , Mitocôndrias/genética , Plastídeos/genética , Proteínas Ribossômicas/genética , Ribossomos/genética , Evolução Biológica , Membrana Celular/genética , Clorófitas/genética , Cloroplastos/genética , Complexo de Proteínas da Cadeia de Transporte de Elétrons/genética , Metabolismo Energético/genética , Células Eucarióticas/citologia , Evolução Molecular , Proteínas de Membrana/genética , Fotossíntese/genética , Respiração/genéticaRESUMO
Diatoms are microalgae that possess so-called "complex plastids," which evolved by secondary endosymbiosis and are surrounded by four membranes. Thus, in contrast to primary plastids, which are surrounded by only two membranes, nucleus-encoded proteins of complex plastids face additional barriers, i.e., during evolution, mechanisms had to evolve to transport preproteins across all four membranes. This study reveals that there exist glycoproteins not only in primary but also in complex plastids, making transport issues even more complicated, as most translocation machineries are not believed to be able to transport bulky proteins. We show that plastidal reporter proteins with artificial N-glycosylation sites are indeed glycosylated during transport into the complex plastid of the diatom Phaeodactylum tricornutum. Additionally, we identified five endogenous glycoproteins, which are transported into different compartments of the complex plastid. These proteins get N-glycosylated during transport across the outermost plastid membrane and thereafter are transported across the second, third, and fourth plastid membranes in the case of stromal proteins. The results of this study provide insights into the evolutionary pressure on translocation mechanisms and pose unique questions on the operating mode of well-known transport machineries like the translocons of the outer/inner chloroplast membranes (Toc/Tic).
Assuntos
Proteínas de Algas/metabolismo , Diatomáceas/metabolismo , Glicoproteínas/metabolismo , Microalgas/metabolismo , Proteínas de Algas/genética , Evolução Biológica , Cloroplastos/metabolismo , Diatomáceas/genética , Diatomáceas/ultraestrutura , Retículo Endoplasmático/metabolismo , Glicoproteínas/genética , Glicosilação , Membranas Intracelulares/metabolismo , Microalgas/genética , Microalgas/ultraestrutura , Microscopia Imunoeletrônica , Plastídeos/metabolismo , Plastídeos/ultraestrutura , Transporte ProteicoRESUMO
Fungal hyphae and plant pollen tubes are among the most highly polarized cells known and pose extraordinary requirements on their cell polarity machinery. Cellular morphogenesis is driven through the phospholipid-dependent organization at the apical plasma membrane. We characterized the contribution of phosphoinositides (PIs) in hyphal growth of the filamentous ascomycete Neurospora crassa. MSS-4 is an essential gene and its deletion resulted in spherically growing cells that ultimately lyse. Two conditional mss-4-mutants exhibited altered hyphal morphology and aberrant branching at restrictive conditions that were complemented by expression of wild type MSS-4. Recombinant MSS-4 was characterized as a phosphatidylinositolmonophosphate-kinase phosphorylating phosphatidylinositol 4-phosphate (PtdIns4P) to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P(2)). PtdIns3P was also used as a substrate. Sequencing of two conditional mss-4 alleles identified a single substitution of a highly conserved Y750 to N. The biochemical characterization of recombinant protein variants revealed Y750 as critical for PI4P 5-kinase activity of MSS-4 and of plant PI4P 5-kinases. The conditional growth defects of mss-4 mutants were caused by severely reduced activity of MSS-4(Y750N), enabling the formation of only trace amounts of PtdIns(4,5)P(2). In N. crassa hyphae, PtdIns(4,5)P(2) localized predominantly in the plasma membrane of hyphae and along septa. Fluorescence-tagged MSS-4 formed a subapical collar at hyphal tips, localized to constricting septa and accumulated at contact points of fusing N. crassa germlings, indicating MSS-4 is responsible for the formation of relevant pools of PtdIns(4,5)P(2) that control polar and directional growth and septation. N. crassa MSS-4 differs from yeast, plant and mammalian PI4P 5-kinases by containing additional protein domains. The N-terminal domain of N. crassa MSS-4 was required for correct membrane association. The data presented for N. crassa MSS-4 and its roles in hyphal growth are discussed with a comparative perspective on PI-control of polar tip growth in different organismic kingdoms.
Assuntos
1-Fosfatidilinositol 4-Quinase/metabolismo , Fusão Celular , Proteínas Fúngicas/metabolismo , Hifas/metabolismo , Neurospora crassa/enzimologia , Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo , Pólen/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Alelos , Escherichia coli/metabolismo , Proteínas de Fluorescência Verde/metabolismo , Microscopia de Fluorescência/métodos , Modelos Genéticos , Mutagênese , Mutação , Fases de Leitura Aberta , Fenótipo , Fosfatidilinositóis/química , Fosforilação , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , TemperaturaRESUMO
Cryptophyte and chlorarachniophyte algae are transitional forms in the widespread secondary endosymbiotic acquisition of photosynthesis by engulfment of eukaryotic algae. Unlike most secondary plastid-bearing algae, miniaturized versions of the endosymbiont nuclei (nucleomorphs) persist in cryptophytes and chlorarachniophytes. To determine why, and to address other fundamental questions about eukaryote-eukaryote endosymbiosis, we sequenced the nuclear genomes of the cryptophyte Guillardia theta and the chlorarachniophyte Bigelowiella natans. Both genomes have >21,000 protein genes and are intron rich, and B. natans exhibits unprecedented alternative splicing for a single-celled organism. Phylogenomic analyses and subcellular targeting predictions reveal extensive genetic and biochemical mosaicism, with both host- and endosymbiont-derived genes servicing the mitochondrion, the host cell cytosol, the plastid and the remnant endosymbiont cytosol of both algae. Mitochondrion-to-nucleus gene transfer still occurs in both organisms but plastid-to-nucleus and nucleomorph-to-nucleus transfers do not, which explains why a small residue of essential genes remains locked in each nucleomorph.
Assuntos
Núcleo Celular/genética , Cercozoários/genética , Criptófitas/genética , Evolução Molecular , Genoma/genética , Mosaicismo , Simbiose/genética , Proteínas de Algas/genética , Proteínas de Algas/metabolismo , Processamento Alternativo/genética , Cercozoários/citologia , Cercozoários/metabolismo , Criptófitas/citologia , Criptófitas/metabolismo , Citosol/metabolismo , Duplicação Gênica/genética , Transferência Genética Horizontal/genética , Genes Essenciais/genética , Genoma Mitocondrial/genética , Genoma de Planta/genética , Genomas de Plastídeos/genética , Dados de Sequência Molecular , Filogenia , Transporte Proteico , Proteoma/genética , Proteoma/metabolismo , Transcriptoma/genéticaRESUMO
BACKGROUND: Although there are many different expression systems for recombinant production of pharmaceutical proteins, many of these suffer from drawbacks such as yield, cost, complexity of purification, and possible contamination with human pathogens. Microalgae have enormous potential for diverse biotechnological applications and currently attract much attention in the biofuel sector. Still underestimated, though, is the idea of using microalgae as solar-fueled expression system for the production of recombinant proteins. RESULTS: In this study, we show for the first time that completely assembled and functional human IgG antibodies can not only be expressed to high levels in algal systems, but also secreted very efficiently into the culture medium. We engineered the diatom Phaeodactylum tricornutum to synthesize and secrete a human IgG antibody against the Hepatitis B Virus surface protein. As the diatom P. tricornutum is not known to naturally secrete many endogenous proteins, the secreted antibodies are already very pure making extensive purification steps redundant and production extremely cost efficient. CONCLUSIONS: Microalgae combine rapid growth rates with all the advantages of eukaryotic expression systems, and offer great potential for solar-powered, low cost production of pharmaceutical proteins.
Assuntos
Diatomáceas/metabolismo , Imunoglobulina G/metabolismo , Engenharia Genética , Antígenos de Superfície da Hepatite B/imunologia , Humanos , Imunoglobulina G/genética , Plasmócitos/metabolismo , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/genéticaRESUMO
Microalgae are thought to offer great potential as expression system for various industrial, therapeutic and diagnostic recombinant proteins as they combine high growth rates with all benefits of eukaryotic expression systems. Moreover, microalgae exhibit a phototrophic lifestyle like land plants, hence protein expression is fuelled by photosynthesis, which is CO(2)-neutral and involves only low production costs. So far, however, research on algal bioreactors for recombinant protein expression is very rare calling for further investigations in this highly promising field. In this study, we present data on the expression of a monoclonal human IgG antibody against the Hepatitis B surface protein and the respective antigen in the diatom Phaeodactylum tricornutum. Antibodies are fully-assembled and functional and accumulate to 8.7% of total soluble protein, which complies with 21 mg antibody per gram algal dry weight. The Hepatitis B surface protein is functional as well and is recognized by algae-produced and commercial antibodies.
Assuntos
Anticorpos/química , Diatomáceas/metabolismo , Microalgas/fisiologia , Anticorpos Monoclonais/química , Bioquímica/métodos , Linhagem Celular , Códon , Ensaio de Imunoadsorção Enzimática/métodos , Proteínas de Fluorescência Verde/metabolismo , Antígenos de Superfície da Hepatite B/metabolismo , Humanos , Imunoglobulina G/química , Microscopia de Fluorescência/métodos , Plasmídeos/metabolismo , Engenharia de Proteínas/métodos , Proteínas Recombinantes/químicaRESUMO
BACKGROUND: Poly-3-hydroxybutyrate (PHB) is a polyester with thermoplastic properties that is naturally occurring and produced by such bacteria as Ralstonia eutropha H16 and Bacillus megaterium. In contrast to currently utilized plastics and most synthetic polymers, PHB is biodegradable, and its production is not dependent on fossil resources making this bioplastic interesting for various industrial applications. RESULTS: In this study, we report on introducing the bacterial PHB pathway of R. eutropha H16 into the diatom Phaeodactylum tricornutum, thereby demonstrating for the first time that PHB production is feasible in a microalgal system. Expression of the bacterial enzymes was sufficient to result in PHB levels of up to 10.6% of algal dry weight. The bioplastic accumulated in granule-like structures in the cytosol of the cells, as shown by light and electron microscopy. CONCLUSIONS: Our studies demonstrate the great potential of microalgae like the diatom P. tricornutum to serve as solar-powered expression factories and reveal great advantages compared to plant based production systems.
Assuntos
Biotecnologia/métodos , Cupriavidus necator/enzimologia , Diatomáceas/metabolismo , Engenharia Genética , Hidroxibutiratos/metabolismo , Microalgas/metabolismo , Poliésteres/metabolismo , Aciltransferases/genética , Aciltransferases/metabolismo , Oxirredutases do Álcool/genética , Oxirredutases do Álcool/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Reatores Biológicos , Biotecnologia/instrumentação , Cupriavidus necator/genética , Diatomáceas/genética , Expressão Gênica , Microalgas/genéticaRESUMO
At first glance the three eukaryotic protein translocation machineries--the ER-associated degradation (ERAD) transport apparatus of the endoplasmic reticulum, the peroxisomal importomer and SELMA, the pre-protein translocator of complex plastids--appear quite different. However, mechanistic comparisons and phylogenetic analyses presented here suggest that all three translocation machineries share a common ancestral origin, which highlights the recycling of pre-existing components as an effective evolutionary driving force. Editor's suggested further reading in BioEssays ERAD ubiquitin ligases Abstract.
Assuntos
Retículo Endoplasmático/metabolismo , Células Eucarióticas/metabolismo , Peroxissomos/metabolismo , Transporte Proteico/fisiologia , Evolução Biológica , Citosol/metabolismo , Funções Verossimilhança , Modelos Biológicos , Plastídeos/metabolismoRESUMO
The regulation of pollen tube growth by the phospholipid phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P(2) ) is not well understood. The Arabidopsis genome encodes two type A phosphatidylinositol-4-phosphate (PI4P) 5-kinases, PIP5K10 and PIP5K11, which are exclusively expressed in pollen and produce PtdIns(4,5)P(2) in vitro. Fluorescence-tagged PIP5K10 and PIP5K11 localized to lateral subapical plasma membrane microdomains in tobacco pollen tubes in a pattern closely resembling the distribution of PtdIns(4,5)P(2,) with the exception of notably weaker association at the extreme apex. Overexpression of PIP5K10 or PIP5K11 in tobacco pollen tubes resulted in severe tip swelling and altered actin fine structure similar to that reported for overexpression of tobacco Nt-Rac5, a monomeric GTPase known to regulate the actin cytoskeleton. Increased sensitivity of Arabidopsis pip5k10 pip5k11 double mutant pollen tubes to Latrunculin B (LatB) further supports a role for type A PI4P 5-kinases in controlling the actin cytoskeleton. Despite the disruption of both its type A PI4P 5-kinases, the pip5k10 pip5k11 double mutant was fertile, indicating that one of the remaining type B PI4P 5-kinase isoforms might be functionally redundant with PIP5K10 and PIP5K11. Antagonistic effects of PIP5K11 and the Nt-Rac5-specific guanine nucleotide dissociation inhibitor, Nt-RhoGDI2, on tip swelling observed in coexpression-titration experiments indicate a link between PtdIns(4,5)P(2) and Rac-signaling in pollen tubes. The data suggest that type A PI4P 5-kinases influence the actin cytoskeleton in pollen tubes in part by counteracting Nt-RhoGDI2, possibly contributing to the control of the pool of plasma membrane-associated Nt-Rac5.
Assuntos
Arabidopsis/crescimento & desenvolvimento , Nicotiana/crescimento & desenvolvimento , Fosfatidilinositol 4,5-Difosfato/metabolismo , Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo , Tubo Polínico/crescimento & desenvolvimento , Proteínas rac de Ligação ao GTP/metabolismo , Actinas/química , Arabidopsis/efeitos dos fármacos , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Compostos Bicíclicos Heterocíclicos com Pontes/farmacologia , Membrana Celular/metabolismo , DNA Bacteriano/genética , Inibidores de Dissociação do Nucleotídeo Guanina/genética , Inibidores de Dissociação do Nucleotídeo Guanina/metabolismo , Mutagênese Insercional , Fosfotransferases (Aceptor do Grupo Álcool)/genética , Fosfotransferases (Aceptor do Grupo Álcool)/farmacologia , Proteínas de Plantas/genética , Proteínas de Plantas/fisiologia , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/crescimento & desenvolvimento , Plantas Geneticamente Modificadas/metabolismo , Tubo Polínico/genética , Tubo Polínico/metabolismo , Tiazolidinas/farmacologia , Nicotiana/genética , Nicotiana/metabolismo , Proteínas rac de Ligação ao GTP/antagonistas & inibidores , Proteínas rac de Ligação ao GTP/genéticaRESUMO
The plastids of cryptophytes, haptophytes, and heterokontophytes (stramenopiles) (together once known as chromists) are surrounded by four membranes, reflecting the origin of these plastids through secondary endosymbiosis. They share this trait with apicomplexans, which are alveolates, the plastids of which have been suggested to stem from the same secondary symbiotic event and therefore form a phylogenetic clade, the chromalveolates. The chromists are quantitatively the most important eukaryotic contributors to primary production in marine ecosystems. The mechanisms of protein import across their four plastid membranes are still poorly understood. Components of an endoplasmic reticulum-associated degradation (ERAD) machinery in cryptophytes, partially encoded by the reduced genome of the secondary symbiont (the nucleomorph), are implicated in protein transport across the second outermost plastid membrane. Here, we show that the haptophyte Emiliania huxleyi, like cryptophytes, stramenopiles, and apicomplexans, possesses a nuclear-encoded symbiont-specific ERAD machinery (SELMA, symbiont-specific ERAD-like machinery) in addition to the host ERAD system, with targeting signals that are able to direct green fluorescent protein or yellow fluorescent protein to the predicted cellular localization in transformed cells of the stramenopile Phaeodactylum tricornutum. Phylogenies of the duplicated ERAD factors reveal that all SELMA components trace back to a red algal origin. In contrast, the host copies of cryptophytes and haptophytes associate with the green lineage to the exclusion of stramenopiles and alveolates. Although all chromalveolates with four membrane-bound plastids possess the SELMA system, this has apparently not arisen in a single endosymbiotic event. Thus, our data do not support the chromalveolate hypothesis.