RESUMO
Since 2001 the Pig Health Unit of Utrecht University has been consulted by various pig farms regarding neonatal diarrhoea. When preventive measures against E. coli-induced diarrhoea had no or limited results, the diarrhoeic piglets were investigated further. The microbiological and pathological findings were indicative of infection with Clostridium perfringens. Toxin typing by polymerase chain reaction led to the detection of genes encoding a-toxin (cpa) and beta2-toxin (cpb2). Surprisingly, alpha- and beta2-toxin-producing C. perfringens was isolated from all tested herds with piglets with neonatal diarrhoea. From our observations, it is likely that many herds in the Netherlands are infected with beta2-toxin-producing C. perfringens strains. As present vaccines lack beta2-toxoid and thus do not provide piglets with protection against beta2-induced diarrhoea.
Assuntos
Toxinas Bacterianas/biossíntese , Infecções por Clostridium/veterinária , Clostridium perfringens/metabolismo , Diarreia/veterinária , Doenças dos Suínos/microbiologia , Animais , Animais Recém-Nascidos/microbiologia , Toxinas Bacterianas/genética , Toxinas Bacterianas/isolamento & purificação , Proteínas de Ligação ao Cálcio/genética , Proteínas de Ligação ao Cálcio/isolamento & purificação , Infecções por Clostridium/epidemiologia , Infecções por Clostridium/microbiologia , Infecções por Clostridium/prevenção & controle , Diarreia/epidemiologia , Diarreia/microbiologia , Diarreia/prevenção & controle , Surtos de Doenças/veterinária , Países Baixos/epidemiologia , Reação em Cadeia da Polimerase , Suínos , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/prevenção & controle , Fosfolipases Tipo C/genética , Fosfolipases Tipo C/isolamento & purificação , Vacinação/veterináriaRESUMO
A 14-month-old Yorkshire boar was examined. In the abdomen, two big tumour masses were found in the intestines. In addition, pale nodules occurred in the liver, kidneys, in and upon the spleen, in the wall of the heart and in the testicles. By immunohistochemistry tumour cells were positive for CD3, but negative for B-lymphocyte antigen and CD79. From these findings it was concluded that the boar was suffering from multicentric lymphosarcoma probably of T-cell origin.