[Organoids for the advancement of intraoperative diagnostic procedures]. / Organoide zur Weiterentwicklung der intraoperativen Diagnostik.

In the context of cancer surgery, there is always a trade-off between oncological safety and preservation of function. This is especially true in pelvic surgery due to the close relationship to the pelvic floor muscles, blood supply and nerves. Currently, risk models, preoperative imaging, the surgeon's assessment, and the intraoperative frozen section serve as the basis for decision-making. New imaging techniques and standardization in frozen section have significantly improved this in recent years. However, limitations remain due to time delays as well as more difficult correct anatomical assignment in the follow-up. Alternative intraoperative techniques may overcome this limitation in the future. Patient-derived organoids have emerged as an important new research vehicle in recent years. They are based on tumor stem cells that, under special culture conditions, form three-dimensional replicas of the original tissue. This makes them ideally suited for testing individual system therapies but also as a validation technique for new intraoperative diagnostic procedures. The Research Training Group 2543/I, which is funded by the German Research Foundation, is researching the potential of new diagnostic methods in an interdisciplinary team regarding validation in addition to intraoperative frozen sections.

Near-infrared optical investigations of snow, ice, and water layers on diffuse reflecting surfaces.

While most experiments on water or ice utilize rather complex, elaborate, and expensive apparatus in order to obtain reliable optical data, here we present a simple and affordable setup that enables us to perform near-infrared measurements on water, ice, and snow on top of rough diffuse reflecting surfaces such as concrete, stone, pavement, or asphalt. By using the properties of diffuse scattering instead of specular reflection, we are able to determine the imaginary part of the refraction index of water without using any liquid cells. In addition, we demonstrate that the snow spectra can be well described by newly developed two-dimensional ray tracing simulations.

Rotationally symmetric formulation of the wave propagation method-application to the straylight analysis of diffractive lenses.

We introduce a modified formulation of the wave propagation method for the efficient simulation of rotationally symmetric micro-optical components. The reformulated algorithm provides an increased computational performance of approximately two orders of magnitude and strongly reduced memory requirements, in comparison to the original formulation. This enables the efficient wave optical simulation of extended micro-optical structures beyond the common thin-element approximation. As a prototypical example, we assess the modified algorithm for the evaluation of straylight induced by diffractive lenses. We find an excellent accuracy, while comparing to rigorous simulations, which justifies the ability to overcome the limitations of the thin-element approximation.

Bimodal endoscopic probe combining white-light microscopy and optical coherence tomography.

We present a novel bimodal endoscopic imaging probe that can simultaneously provide full-field white-light video microscopy and confocal optical coherence tomography (OCT) depth scans. The two modalities rely on spectrally separated optical paths that run partially in parallel through a micro-optical bench system, which has a cross-section of only 2 mm×2.76 mm and is realized via standard silicon micromachining techniques. With a numerical aperture of 0.061, the video modality has a resolution and field of view of 9.3 and 1240 µm×1080 µm, respectively. The resolution is limited by the pixel spacing of the coherent fiber bundle, which relays the acquired image from the distal to the proximal end. A custom-designed diffractive optical element placed within the video imaging path significantly improves the image contrast by up to 45% in the medium frequency range. The OCT modality is optimized for 830 nm center wavelength, and works in a confocal arrangement with an NA of 0.018. It provides single-point depth probing at the center of the video image with a lateral resolution of 20 µm. Through its compact footprint and enhanced functionality, the probe can provide depth-resolved guiding capability for existing laparoscopes and represents a major step toward a new class of multimodal endoscopic imaging probes.

Wave-optical design of a combined refractive-diffractive varifocal lens.

A novel type of integrated refractive-diffractive varifocal membrane lens is designed and analyzed by wave-optical methods. In contrast to other hybrid devices, the diffractive microstructure is directly imprinted onto the soft deflecting membrane, allowing for a high level of integration. Elastic deformation is taken into account by mechanical simulations with the finite element method (FEM). We show, that the superimposed structure can considerably suppress chromatic and spherical aberration. Furthermore, our algorithm is successfully applied to design a confocal hyperspectral lens.