RESUMO
The manifestation of periodontitis-related inflammatory reaction is inevitably bound to the production of prostaglandins E2 and D2 which have been suggested to mediate osteoclastic and osteogenic effects within the affected tissue. We demonstrated the presence of PGE2 and PGD2 receptors on hMSCs on RNA level and with immunofluorescence. For each Prostaglandin, three concentrations were studied: 0.1; 0.5 or 1.0⯵g/ml. A lower expression of EP1 and EP4 (PGE2 receptors 1 and 4) after stimulation with PGE2 was shown, thus a tendency to compromise osteogenic differentiation and metabolism. PGE2 induced a higher growth-rate during the first week, while a continuous inflammatory challenge determined a decrease of the proliferation of hMSCs. PGD2 inhibited cell growth irrespective of the duration of the stimulation. PGE2 and PGD2 have also negative effects on calcium deposition osteogenic, thus on differentiation of hMSCs. PGE2 and PGD2 seem to induce bone resorption also having indirectly a negative impact on the osteogenic differentiation of hMSCs. Thus, inhibitors of PGE2 and PGD2 can be used as adjunct to mechanical periodontal treatment.
Assuntos
Dinoprostona/farmacologia , Células-Tronco Mesenquimais/citologia , Osteogênese/efeitos dos fármacos , Prostaglandina D2/farmacologia , Cálcio/metabolismo , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Relação Dose-Resposta a Droga , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Masculino , Células-Tronco Mesenquimais/química , Células-Tronco Mesenquimais/efeitos dos fármacos , Receptores Imunológicos/genética , Receptores de Prostaglandina/genética , Receptores de Prostaglandina E/genéticaAssuntos
Arquitetura de Instituições de Saúde/legislação & jurisprudência , Casas de Saúde/legislação & jurisprudência , Acessibilidade Arquitetônica/legislação & jurisprudência , Códigos de Obras , Arquitetura de Instituições de Saúde/economia , Humanos , Massachusetts , Negociação , Casas de Saúde/economiaRESUMO
The Boston Home's mission, to preserve the dignity and autonomy of its residents, was achieved through thoughtful design. The updated facility is a "true success story" and serves as an example of what can be achieved when residents and staff work together.