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1.
Front Cell Infect Microbiol ; 14: 1194307, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38343886

RESUMO

A 51-year-old male with a history of Cacchi-Ricci disease and long-standing infection with various species of Borrelia, Babesia, and Bartonella presented with recurrent symptoms of right-sided flank pain. Numerous renal calculi were identified on imaging. The etiology of the calculi had not been previously elucidated. Symptoms intermittently date back to 2002 when uric acid stones were identified. Subsequent calculi analysis revealed calcium oxalate stones. Despite the commonality of nephrolithiasis in patients with Cacchi-Ricci disease, the extreme number of calculi and recurrent presentation of symptoms persisted despite a plethora of medical evaluations, dietary changes, and hereditary testing. This case raises questions of etiology including possible immune deficiency and whether his uncommon microbial history contributes to recurrent stone formation.


Assuntos
Cálculos Renais , Rim em Esponja Medular , Doenças Transmitidas por Carrapatos , Masculino , Humanos , Pessoa de Meia-Idade , Cálculos Renais/complicações , Cálculos Renais/diagnóstico , Rim , Rim em Esponja Medular/complicações , Doenças Transmitidas por Carrapatos/complicações , Doenças Transmitidas por Carrapatos/diagnóstico
2.
Tissue Eng ; 12(2): 245-55, 2006 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16548683

RESUMO

Previous work has shown that keratinocytes can be cultured serum-free on an acid-functionalized, plasma-polymerized surface (for subsequent delivery to patients' wound beds) by inclusion of a fibroblast feeder layer. This study seeks to extend this work by substituting human for murine feeder cells in serum-free culture and examining the performance of keratinocytes expanded in this way to transfer to an in vitro human dermal wound bed model. We compared murine and human fibroblasts (both short-term dermal fibroblasts and a fetal lung fibroblast cell line MRC-5, which has a long history in human vaccine production), alternative methods for growth-arresting fibroblasts, establishing culture of cells serum-free, and the impact of culture with fibroblasts on the differentiation of the keratinocytes. Irradiated human and murine fibroblasts were equally effective in supporting initial keratinocyte expansion, both in the presence and absence of serum. Keratinocytes were significantly less differentiated, as assessed by measuring involucrin expression relative to DNA when grown serum-free with fibroblasts than when grown with serum. Initial cultures of fibroblasts and keratinocytes could be initiated serum-free but were much slower to establish than if serum were used. Transfer of keratinocytes from keratinocyte/fibroblast co-cultures cultured on a plasma polymer surface to a human dermal wound bed model was as successful as from monocultures in both serum and serum-free cultures. In summary, we have revisited a well-accepted methodology for expanding human keratinocytes for clinical use and avoided the use of bovine serum and a mouse fibroblast feeder layer by introducing an irradiated human fibroblast feeder layer.


Assuntos
Fibroblastos/metabolismo , Queratinócitos/metabolismo , Diferenciação Celular , Linhagem Celular , Proliferação de Células , Técnicas de Cocultura , Colágeno Tipo I/metabolismo , Meios de Cultura Livres de Soro , Fluoresceína-5-Isotiocianato , Técnica Indireta de Fluorescência para Anticorpo , Corantes Fluorescentes , Raios gama , Humanos , Microscopia de Fluorescência , Mitomicina/farmacologia , Polímeros/química , Precursores de Proteínas/metabolismo , Especificidade por Substrato
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