RESUMO
In Penicillium chrysogenum, key enzymes involved in the production of penicillin reside in peroxisomes. As a first step to understand the role of these organelles in penicillin biosynthesis, we set out to isolate the genes involved in peroxisome biogenesis. Here we report the cloning and characterization of P. chrysogenum PEX1 and PEX6, which encode proteins of the AAA family of ATPases. The second AAA module, which is essential for the function of Pex1p and Pex6p in peroxisome biogenesis, is highly conserved in both PcPexlp and PcPex6p. PcPEX1 and PcPEX6 contain three and two introns, respectively.
Assuntos
Adenosina Trifosfatases/genética , Proteínas Fúngicas/genética , Penicillium chrysogenum/genética , Peroxissomos/metabolismo , ATPases Associadas a Diversas Atividades Celulares , Adenosina Trifosfatases/química , Adenosina Trifosfatases/metabolismo , Trifosfato de Adenosina/metabolismo , Sítios de Ligação , Clonagem Molecular , Proteínas Fúngicas/química , Proteínas Fúngicas/metabolismo , Genes Fúngicos , Íntrons , Dados de Sequência Molecular , Penicillium chrysogenum/metabolismo , Penicillium chrysogenum/ultraestrutura , Reação em Cadeia da PolimeraseRESUMO
We have cloned the Hansenula polymorpha PEX1 and PEX6 genes by functional complementation of the corresponding peroxisome-deficient (pex) mutants. The gene products, HpPex1p and HpPex6p, are ATPases which both belong to the AAA protein family. Cells deleted for either gene (Deltapex1 or Deltapex6) were characterized by the presence of small peroxisomal remnants which contained peroxisomal membrane proteins and minor amounts of matrix proteins. The bulk of the matrix proteins, however, resided in the cytosol. In cell fractionation studies HpPex1p and HpPex6p co-sedimented with the peroxisomal membrane protein HpPex3p in both wild-type cells and in Deltapex4, Deltapex8 or Deltapex14 cells. Both proteins are loosely membrane-bound and face the cytosol. Furthermore, HpPex1p and HpPex6p physically and functionally interact in vivo. Overexpression of PEX6 resulted in defects in peroxisomal matrix protein import. By contrast, overexpression of PEX1 was not detrimental to the cells. Interestingly, co-overproduction of HpPex1p rescued the protein import defect caused by HpPex6p overproduction. Overproduced HpPex1p and HpPex6p remained predominantly membrane-bound, but only partially co-localized with the peroxisomal membrane protein HpPex3p. Our data indicate that HpPex1p and HpPex6p function in a protein complex associated with the peroxisomal membrane and that overproduced, mislocalized HpPex6p prevents HpPex1p from reaching its site of activity.
Assuntos
Adenosina Trifosfatases/genética , Microcorpos/fisiologia , Pichia/fisiologia , Sequência de Aminoácidos , Animais , Anticorpos Antifúngicos/biossíntese , Sequência de Bases , Southern Blotting , Western Blotting , Clonagem Molecular , Primers do DNA/química , DNA Fúngico/química , Eletroforese em Gel de Poliacrilamida , Eletroporação , Imuno-Histoquímica , Microcorpos/genética , Microcorpos/ultraestrutura , Microscopia Eletrônica , Dados de Sequência Molecular , Mutação , Pichia/genética , Pichia/ultraestrutura , Reação em Cadeia da Polimerase , Testes de Precipitina , Coelhos , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência de AminoácidosRESUMO
We have cloned the Hansenula polymorpha PEX4 gene by functional complementation of a peroxisome-deficient mutant. The PEX4 translation product, Pex4p, is a member of the ubiquitin-conjugating enzyme family. In H.polymorpha, Pex4p is a constitutive, low abundance protein. Both the original mutant and the pex4 deletion strain (Deltapex4) showed a specific defect in import of peroxisomal matrix proteins containing a C-terminal targeting signal (PTS1) and of malate synthase, whose targeting signal is not yet known. Import of the PTS2 protein amine oxidase and the insertion of the peroxisomal membrane proteins Pex3p and Pex14p was not disturbed in Deltapex4 cells. The PTS1 protein import defect in Deltapex4 cells could be suppressed by overproduction of the PTS1 receptor, Pex5p, in a dose-response related manner. In such cells, Pex5p is localized in the cytosol and in peroxisomes. The peroxisome-bound Pex5p specifically accumulated at the inner surface of the peroxisomal membrane and thus differed from Pex5p in wild-type peroxisomes, which is localized throughout the matrix. We hypothesize that in H. polymorpha Pex4p plays an essential role for normal functioning of Pex5p, possibly in mediating recycling of Pex5p from the peroxisome to the cytosol.
Assuntos
Proteínas Fúngicas/metabolismo , Ligases/metabolismo , Pichia/enzimologia , Receptores Citoplasmáticos e Nucleares/metabolismo , Ubiquitinas/metabolismo , Sequência de Aminoácidos , Transporte Biológico , Proteínas Fúngicas/genética , Membranas Intracelulares/metabolismo , Microcorpos/metabolismo , Dados de Sequência Molecular , Mutagênese , Receptor 1 de Sinal de Orientação para Peroxissomos , Pichia/genética , Homologia de Sequência de AminoácidosRESUMO
We have cloned and characterized the Hansenula polymorpha PER9 gene by functional complementation of the per9-1 mutant of H. polymorpha, which is defective in peroxisome biogenesis. The predicted product, Per9p, is a polypeptide of 52 kDa with sequence similarity to Pas3p, a protein involved in peroxisome biogenesis in Saccharomyces cerevisiae. In a per9 disruption strain (Deltaper9), peroxisomal matrix and membrane proteins are present at wild-type levels. The matrix proteins accumulated in the cytoplasm. However, the location of the membrane proteins remained obscure; fully induced Deltaper9 cells lacked residual peroxisomal vesicles ("ghosts"). Analysis of the activity of the PER9 promoter revealed that PER9 expression was low in cells grown on glucose, but was enhanced during growth of cells on peroxisome-inducing substrates. The highest expression levels were observed in cells grown on methanol. Localization studies revealed that Per9p is an integral membrane protein of the peroxisome. Targeting studies suggested that Per9p may be sorted to the peroxisome via the endoplasmic reticulum. Overexpression of PER9 induced a significant increase in the number of peroxisomes per cell, a result that suggests that Per9p may be involved in peroxisome proliferation and/or membrane biosynthesis. When PER9 expression was placed under the control of a strongly regulatable promoter and switched off, peroxisomes were observed to disintegrate over time in a manner that suggested that Per9p may be required for maintenance of the peroxisomal membrane.
Assuntos
Transportadores de Cassetes de Ligação de ATP , Proteínas Fúngicas/genética , Genes Fúngicos , Proteínas de Membrana/genética , Microcorpos/química , Pichia/genética , Proteínas de Saccharomyces cerevisiae , Sequência de Aminoácidos , Clonagem Molecular , DNA Fúngico/genética , Dados de Sequência Molecular , Peroxinas , Mapeamento por Restrição , Alinhamento de Sequência , Homologia de Sequência de AminoácidosRESUMO
We have analyzed the presence of peroxisomal remnants ('ghosts') in three peroxisome-deficient (per) mutants of the yeast Hansenula polymorpha, namely delta per4, delta per5 and delta per10. Under peroxisome-inducing growth conditions peroxisomal membrane proteins (PMPs) were normally synthesized in cells of these mutants. In addition, these cells contained clusters of small membraneous vesicles, which were absent in cells grown under peroxisome-repressing growth conditions. These structures displayed typical peroxisomal properties in that they proliferated upon overproduction of Per8p, the H. polymorpha peroxisome proliferation factor. Moreover, in delta per4 and delta per5 these vesicles were susceptible to glucose-induced proteolytic degradation.
Assuntos
Microcorpos/ultraestrutura , Pichia/ultraestrutura , Western Blotting , Divisão Celular , Proteínas Fúngicas/metabolismo , Glucose/farmacologia , Proteínas de Membrana/metabolismo , Microcorpos/metabolismo , Microscopia Eletrônica , Mutação , Peroxinas , Pichia/genética , Pichia/metabolismoRESUMO
PER genes are essential for the assembly of peroxisomes in Hansenula polymorpha. Here we describe the PER3 gene which was cloned by functional complementation of a H. polymorpha per3 mutant. The complementing PER3 gene encodes a protein of 569 amino acids (Per3p) with a calculated mass of 63.9 kDa; Per3p belongs to the tetratricopeptide repeat protein family and is located in both the cytosol and the peroxisomal matrix. Remarkably, Per3p does not contain a known targeting signal (PTS1 or PTS2). The PER3 gene product shows similarity to the Saccharomyces cerevisiae Pas10p (40% identity) and the Pichia pastoris Pas8p (55% identity). However, their function apparently cannot be interchanged since the P. pastoris PAS8 gene failed to functionally complement a H. polymorpha per3 disruption mutant. The per3 disruption mutant contained normal but small peroxisomes in which PTS2 proteins (both homologous and heterologous) were imported. Other matrix proteins (in particular PTS1 proteins) resided in the cytosol where they were normally assembled and active. We argue that Per3p is a component of the peroxisomal import machinery and most probably shuttles matrix proteins from the cytosol to the organellar matrix.