RESUMO
The possible role of fetal membrane deconjugating activity in the movement of a charged steroid conjugate between fetal and maternal compartments was investigated. The ability of amnion and chorion laeve to transfer [3H]estrone-[35S]sulfate was assessed in both orientations of guinea pig tissue at 45 days and near parturition. While early amnion was impermeable, late tissue transferred approximately 50% (w/w) of the substrate in a bidirectional process that was non-saturable and independent of either deconjugation or ATP. Transfer across early chorion was similar to late amnion. Saturation curves from each tissue were superimposable, as were those of the time course. Transfer across both early and late chorion proceeded in the absence of deconjugation, with no effect of tissue orientation or ATP depletion. However, late chorion exhibited a decrease in estrone-sulfate transfer, as verified by concentration dependency and time course analyses, though transport across the tissue remained non-saturable. The results in amnion were congruous with the presence and absence of tight junctions in the epithelium of early and late tissue, respectively. However, sulfoconjugate transfer across early chorion proceeded in the presence of a paracellular barrier, suggesting specialized regulation of the transport process which extended late into gestation.
Assuntos
Âmnio/fisiologia , Córion/fisiologia , Estrona/análogos & derivados , Animais , Transporte Biológico , Estrogênios Conjugados (USP)/farmacocinética , Estrona/farmacocinética , Feminino , Idade Gestacional , Cobaias , Técnicas In Vitro , Cinética , Permeabilidade , Gravidez , Radioisótopos de Enxofre , TrítioRESUMO
To understand the means whereby a charged, estrogen conjugate may be transferred across guinea pig amnion and chorion, the permeability to [3H]estrone-[14C]glucuronide was examined at 45 days and near term. No evidence of deconjugation was obtained in either early or late amnion, despite significantly greater transfer near term. Early amnion was virtually impermeable, regardless of ATP depletion. In contrast, early chorion transferred estrone-glucuronide without any requirement for deconjugation or ATP. No effect of tissue orientation was observed in amnion; whereas, incubations from maternal to fetal side of late chorion exhibited beta-glucuronidase activity. Inhibition of the latter demonstrated that hydrolysis was concomitant with but not required for transport. [3H]Estrone produced by deconjugation was enzymatically reduced after pubic symphysis relaxation, although beta-glucuronidase activity began prior to this stage. Transport across late fetal membranes was not saturable and chorion incubations from maternal to fetal side demonstrated a lower transport capacity. In either tissue orientation, late chorion displayed a lower rate of transfer than amnion. These results indicate that fetal membranes possess distinct abilities for transferring intact estrone-glucuronide, depending on stage of development and tissue orientation. The passive nature of transport and its dependence on structural characteristics is consistent with possible regulation of tight junctions.
Assuntos
Permeabilidade da Membrana Celular/fisiologia , Estrogênios Conjugados (USP)/metabolismo , Estrona/análogos & derivados , Feto/enzimologia , Glucuronatos/metabolismo , Glucuronidase/fisiologia , Âmnio/fisiologia , Animais , Transporte Biológico/fisiologia , Radioisótopos de Carbono/metabolismo , Córion/enzimologia , Córion/fisiologia , Cobaias , Trítio/metabolismoRESUMO
Transfer of steroidal and nonsteroidal compounds across guinea pig amnion and chorion laeve was investigated as a function of stage of gestation, tissue orientation, steroid specificity, and molecular size. Each fetal membrane was examined at early and late stages of gestation, before and after pubic symphysis relaxation. Early amnion was impermeable to macromolecules and small charged molecules while [3H]estrone and [3H]pregnenolone were transferred, the latter depending on tissue orientation and involving conjugation at the basolateral interface. After symphysis dilation, amnion transferred all substrates tested with the exception of BSA; the molecular weight cutoff was approximately 5,000. Unlike amnion, early chorion transferred both free and conjugated steroids as well as inorganic sulfate. Transfer of estrone involved conjugation and depended on tissue orientation. Transfer of [3H]estrone-sulfate, [3H]estrone-glucuronide, and [3H]pregnenolone-sulfate was similar despite selective deconjugating activity toward estrone-sulfate. Near term, chorion was impermeable to inorganic sulfate and transfer of estrone-glucuronide depended on tissue orientation, involving deconjugation in the maternal to fetal direction. At no stage of gestation did chorion transfer macromolecules. These results suggest that the transfer of free and conjugated steroids across fetal membranes is differentially regulated by tissue, its stage of development, and direction of transfer.
Assuntos
Estrogênios Conjugados (USP)/metabolismo , Membranas Extraembrionárias/metabolismo , Esteroides/metabolismo , Âmnio/metabolismo , Animais , Córion/metabolismo , Estrona/análogos & derivados , Estrona/metabolismo , Feminino , Idade Gestacional , Cobaias , Gravidez , Pregnenolona/metabolismoRESUMO
The in vitro fate of [3H]progesterone was studied after incubation with guinea pig tissues at 58/59 days (before pubic symphysis relaxation) and in the final week (post relaxation) of gestation. Buffered steroid was added to the fetal surface of chorion attached to the uterus or to the uterus alone. Neither the amount of recovered progesterone nor its metabolites (6.2% average conversion) differed between the two stages when only uterus was incubated. With chorion present, conversion averaged 28.3% at 58/59 days and 63.4% at the late stage. A 4.6-fold decrease in progesterone concentration, and 3.0-, 2.4- and 3.1-fold increases in the concentrations of 3alpha-hydroxy-5alpha-pregnan-20-one, 3beta-hydroxy-5alpha-pregnan-20-one and 5alpha-pregnane-3,20-dione, respectively, were found in the uterus in the late stage vs 58/59 days. Also, 2.8- and 6.4-fold decreases in progesterone concentration occurred in the myometrium and endometrium, respectively, from 58/59 days to the late stage. In endometrium, the concentrations of the 3alpha- and 3beta-isomers, and 5alpha-pregnane-3,20-dione, increased 2.6-, 2.6- and 5.0-fold, respectively. The above changes were all significant at P < 0.05 or better. Changes in the 3alpha-, 3beta- isomers and dione in the myometrium were not significant. The chorion-uterine interaction and gestation time thus affect the degree of progesterone conversion, and the amounts of metabolites reaching the uterus in the chorion-uterine in vitro system.
Assuntos
Córion/embriologia , Desenvolvimento Embrionário e Fetal , Progesterona/metabolismo , Útero/metabolismo , Animais , Córion/metabolismo , Feminino , Cobaias , Troca Materno-Fetal , GravidezRESUMO
The activities of estrogen sulfotransferase, estrogen sulfatase and estradiol 17beta-dehydrogenase change considerably in the guinea pig uterine compartment during gestation. This study was undertaken to inquire if the chorion membrane could influence the pattern of estrogen resulting when substrates were applied to the fetal surface of the chorion while it was attached, late in gestation, to the uterine wall. This tissue system resulted in a differential handling of estrone and estradiol. Estrone was largely excluded from the tissue, remaining mainly in free steroidal form. Estradiol was considerably converted to its 3-sulfate which was mainly retained by the chorion. Parallel experiments with chorion and uterus separately failed to discriminate between the two substrates. Hydrolysis of estrone sulfate and estradiol 3-sulfate was similar in all three tissue systems. It appears that the interaction of chorion with uterus late in gestation causes a difference in tissue action towards the two steroid substrates of closely related structure. The results suggest a limitation in tissue uptake of estrone compared with estradiol, or a much greater sulfotransferase activity towards estradiol. Whole cytosols of late gestational chorion catalyzed sulfation of estradiol at about double the velocity of estrone. This may only partly account for the difference in the intact chorion-uterine tissue system.
Assuntos
Córion/metabolismo , Estradiol/metabolismo , Estrona/metabolismo , Sulfatos/metabolismo , Útero/metabolismo , Animais , Córion/citologia , Citosol/metabolismo , Feminino , Cobaias , Gravidez , Especificidade por SubstratoRESUMO
Tissue viability and permeability of guinea pig amnion and chorion leave were analyzed microscopically and biochemically. The vital dyes T1111 and fluorescein diacetate were used to locate and determine the integrity of cell plasma membranes in early and late tissue in vitro using confocal laser scanning microscopy and scanning electron microscopy. Early amnion and chorion laeve were each found to contain a single epithelial cell layer, composed of membrane-intact cells. In contrast, plasma membrane lesions were present throughout the epithelium of late amnion. Late chorion laeve contained both regions of intact and damaged epithelial cells on its maternal side. There was also a layer of membrane-intact squamous cells on the fetal side of late chorion laeve. ATP measurements confirmed that early fetal membranes were viable after incubation in isotonic salt solutions at physiological pH. Late amnion was depleted of ATP stores while late chorion laeve retained its capacity for generating energy. These viability markers indicate that late guinea pig amnion is not a viable tissue in vitro, while late chorion laeve is a viable but probably degenerating tissue. Confocal X-Z scans were used to trace the movement of T1111 through the tissue as an indication of permeability to free solutes. Whereas dye will permeate across the main thickness of early amnion and chorion leave, it did not pass between cells, but was blocked, presumably by a line of tight junctions. Late amnion was characterized by the complete permeability to T1111. Late chorion leave contained regions where solute migration was blocked, but overall was a permeable tissue. These results provide an important context for the interpretation of molecular movement across fetal membranes.
Assuntos
Âmnio/ultraestrutura , Córion/ultraestrutura , Trifosfato de Adenosina/análise , Âmnio/citologia , Âmnio/metabolismo , Animais , Membrana Celular/metabolismo , Membrana Celular/ultraestrutura , Córion/citologia , Córion/metabolismo , Membranas Extraembrionárias/metabolismo , Membranas Extraembrionárias/fisiologia , Membranas Extraembrionárias/ultraestrutura , Feminino , Fluoresceínas/análise , Cobaias , Microscopia Confocal , Microscopia Eletrônica de Varredura , Permeabilidade , GravidezRESUMO
Progesterone metabolism by guinea pig amnion, chorion, myometrium, and endometrium was studied at the following gestational stages. Day 45 represents mid-gestation, about 5 days before strong chorion interaction between the entire surface of the chorion and the uterus; days 57-58, 1-2 days after chorion attachment, and 2-3 days before the onset of pubic symphysis relaxation; days +1-+6, 1-6 days after the onset of pubic symphysis relaxation, i.e. within 1 week of parturition. The high metabolic activity of chorion exceeded that by amnion at all stages. Metabolism by endometrium and myometrium was always low. Conversion of progesterone by amnion significantly decreased (P < 0.05) between days 57-58 and days +1-+6. Progesterone metabolites produced by chorion and amnion were identified by TLC, HPLC, and capillary GC/MS. Both tissues converted progesterone to three major products during 60-min incubations. These were 5 alpha-pregnane-3,20-dione, 3 alpha-hydroxy-5 alpha-pregnan-20-one, and 3 beta-hydroxy-5 alpha-pregnan-20-one. The metabolite pattern differed between the two tissues. Three-minute incubations with chorion resulted in a significantly higher proportion of 3 alpha-hydroxy-4-pregnen-20-one (P < 0.01) and 5 alpha-pregnane-3,20-dione (P < 0.025) than at 60 min. The production of 3 beta-hydroxy-5 alpha-pregnen-20-one by chorion decreased (P < 0.05) between days 50-51 and 57-58. The ratio of 3 alpha-hydroxy-5 alpha-pregnan-20-one to 3 beta-hydroxy-5 alpha-pregnan-20-one increased (P < 0.05) between days 45 post-relaxation. The marked conversion of progesterone by chorion, or the formation of one or more of its metabolites, may serve to influence uterine function prior to delivery.
Assuntos
Progesterona/metabolismo , Útero/metabolismo , Âmnio/metabolismo , Animais , Córion/metabolismo , Cromatografia Líquida de Alta Pressão , Endométrio/metabolismo , Feminino , Cromatografia Gasosa-Espectrometria de Massas , Cobaias , Técnicas In Vitro , Miométrio/metabolismo , Gravidez , Fatores de TempoRESUMO
Because progesterone suppresses myometrial contractility, the assumption is often made that the withdrawal of this steroid is a prerequisite for parturition. However, steroid patterns in maternal blood of the guinea pig do not consistently change with impending parturition and it has been claimed that progesterone does not suppress guinea pig myometrial contraction. The present study investigated progesterone and estrogen nuclear receptor binding in myometrium, endometrium, and chorion between 32 days of gestation and delivery at 67-71 days. Binding characteristics and behavior during sedimentation in sucrose density gradients were typical of the steroid hormone receptor family. Decreased progestin binding occurred in the myometrium, from a high of 1600 fmol/mg DNA at 49-51 days to a low of 450 fmol/mg DNA (P < 0.01) on the day of detectable pubic relaxation. This decrease commenced at 60-63 days just before the onset of relaxation. A similar, though less well defined change occurred in endometrium. Estradiol nuclear receptor binding in myometrium remained at about 350 fmol/mg DNA from 32 days until 1-2 days pre-partum when it increased to about 650 fmol/mg DNA (P < 0.05). Estradiol binding in endometrium showed an inconsistent pattern and chorion binding for both progestin and estradiol was low and unremarkable. We conclude that there is a potential for decreased progesterone effect in myometrium at about one week before delivery and increased estrogen action in that tissue immediately before delivery.
Assuntos
Núcleo Celular/metabolismo , Prenhez/metabolismo , Receptores de Estradiol/metabolismo , Receptores de Progesterona/metabolismo , Útero/metabolismo , Animais , DNA/metabolismo , Estradiol/metabolismo , Feminino , Cobaias , Gravidez , Promegestona/metabolismo , Fatores de Tempo , Trítio , Útero/ultraestruturaRESUMO
Formation of steroid sulfates is catalyzed by sulfotransferase enzymes that are widely distributed and frequently of high specificity. Steroid sulfates cannot be described as being active hormones, but some serve in tissue sites as precursors of active steroids formed by enzymic cleavage of the sulfate group by sulfatase enzymes. There is increasing evidence that intracellular sulfation and desulfation can play a role in regulating the availability of active steroid hormones near target sites. There are strong indications for this regulation in the uterine compartment, in the liver, and in mammary cancer cells.
RESUMO
Between about 50 and 58 days of gestation, the guinea pig chorion becomes attached in its entirety to the uterine wall, suggesting a facilitation of transfer of agents such as steroids between these tissues. At a time between 59 and 64 days, relaxation of the pubic symphysis starts, and anywhere from 5 to 8 days after that event delivery takes place. The present in vitro study was undertaken to evaluate estrone sulfate as a substrate for local production of estradiol, via the action of estrogen sulfatase and 17-hydroxysteroid dehydrogenase, in chorion, endometrium and myometrium taken at four distinct stages of gestation, as follows: 50 days, representing pre-chorion attachment to the uterus (stage 50); 1 or 2 days before pubic symphysis relaxation (minus 1 day, or -1 day); 1 day following relaxation (+1 day); and 1-2 days before delivery (late, or L). At these same stages, the metabolite patterns formed from estradiol were evaluated for endometrium and myometrium. Each of the tissues behaved somewhat differently. Overall hydrolysis of estrone sulfate by endometrium and myometrium exceeded that by chorion. Generation of free steroid from estrone sulfate increased 3-fold in chorion between stages 50 and -1 and during this period estradiol production from estrone sulfate increased 9-fold and continued to rise until delivery. Cytosolic estrogen sulfotransferase activity of chorion decreased 7-fold between stages 50 and -1. This suggested a tissue environment geared to producing potentially active estradiol. However, myometrium converted very little estrone into estradiol until just before delivery despite the facile formation of estrone from estradiol at stages -1, +1 and L. The control of estrogen metabolism by interaction of tissues in the uterus and by some form of enzyme regulation in these tissues suggests a possible role for locally produced estrogen in the stages leading up to parturition.
Assuntos
Estradiol/biossíntese , Miométrio/enzimologia , Prenhez/metabolismo , Útero/enzimologia , 17-Hidroxiesteroide Desidrogenases/metabolismo , Animais , Córion/enzimologia , Endométrio/enzimologia , Estrona/análogos & derivados , Estrona/metabolismo , Feminino , Cobaias , Gravidez , Sulfatases/metabolismo , Fatores de TempoRESUMO
The highest total activity of estrogen sulfotransferase in guinea pig is in liver and the highest specific activities are in the adrenal and the midgestational chorion. Guinea pig gonads contain scarcely detectable activities. In CD-1 mice the highest specific activity is in testis and the highest total activity is in late placenta. Adrenals from both sexes and ovaries contain minimal activities, while liver and fetal membrane activities are remarkably low. In CD-1, DBA, C57BL, and BALB mice, qualitative patterns are similar. Purified or partially purified estrogen sulfotransferase from guinea pig adrenal and chorion were used to study the effect of a number of possible steroidal inhibitors. Considerable structural specificity is evident within a range of steroids, even among some which are not substrates. Pregnenolone is the most effective 21-carbon inhibitor and, in general, more highly hydroxylated forms are less inhibitory. Within a series of 21-, 19- and 18-carbon steroids, the structure of the A ring appears to be extremely important in regard to inhibitory effects.
Assuntos
Cobaias/metabolismo , Camundongos/metabolismo , Esteroides/farmacologia , Sulfotransferases , Sulfurtransferases/análise , Glândulas Suprarrenais/enzimologia , Animais , Córion/enzimologia , Feminino , Genitália Feminina/enzimologia , Fígado/enzimologia , Masculino , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos DBA , Especificidade de Órgãos , Gravidez , Pregnenolona/farmacologia , Sulfurtransferases/antagonistas & inibidores , Testículo/enzimologiaRESUMO
Guinea pig adrenal estrogen sulfotransferase from either sex was eluted as a single peak, irrespective of buffer salt concentration, when subjected to fast protein liquid chromatography on gel filtration columns. The same enzyme was consistently eluted in two distinct peaks during chromatofocusing. Adrenal pregnenolone sulfotransferase was eluted during gel filtration in a heterogeneous pattern, dependent on salt concentration. These properties have made possible almost complete separation of the two sulfotransferases in one step, although adrenal estrogen sulfotransferase may possess a minute intrinsic ability to catalyze sulfation of pregnenolone. Pregnenolone sulfotransferase had no measurable activity toward estrone. Pregnenolone sulfotransferase from both sexes yielded variable elution patterns during chromatofocusing. Estrogen sulfotransferase from the adrenal, as well as that of guinea pig chorion, was strongly inhibited by N-ethylmaleimide and to a lesser degree by iodoacetamide and iodoacetate. Adrenal and chorion estrogen sulfotransferases were thermolabile and were activated, although not protected from the effect of heat, by binding to 3'-phosphoadenosine 5'-phosphosulfate. Adrenal pregnenolone sulfotransferase was inhibited only by high concentrations of N-ethylmaleimide and not at all by iodoacetamide or iodoacetate. It was more thermostable than the estrogen sulfotransferase and was not activated by binding to 3'-phosphoadenosine 5'-phosphosulfate.
Assuntos
Sulfotransferases/análise , Sulfurtransferases/análise , Animais , Cromatografia de Afinidade , Cromatografia em Gel , Citosol/química , Estabilidade Enzimática , Feminino , Cobaias , Temperatura Alta , Masculino , Sulfotransferases/antagonistas & inibidores , Sulfurtransferases/antagonistas & inibidoresRESUMO
Estrogen sulfotransferase (EST) activity measured under optimal in vitro conditions in the 105,000 g cytosols (HSS) of homogenized intrauterine tissues (amnion, chorion, endometrium, decidua basalis and placenta) from guinea-pigs at the 50th day of gestation indicated that the highest specific activity occurred in the chorion. EST activity in the chorion increased from day 34 (early gestation) to peak around day 45 (mid-gestation), before significantly decreasing around day 50 and further declining to barely detectable levels beyond day 60 (late gestation, the onset of parturition). 17 beta-Hydroxysteroid dehydrogenase (17 beta-HSD) activity in the chorion was almost completely membrane associated. The specific activity of the 17 beta-HSD reduction reaction in the 105,000 g pellet was 2.5-fold higher at mid-gestation than at late gestation, while the specific activity of the 17 beta-HSD oxidation reaction was 1.7-fold higher at mid-gestation as compared with late gestation. When intact pieces of chorion tissue from mid- and late gestation were incubated with 5 nM [3H]estradiol (E2), approx. 80% of the recovered free estrogen was E1 (estrone). Only chorion from animals at the onset of parturition were able to produce detectable amounts of E2 from 5 nM [3H]E1. Under the same experimental conditions the ratio of estradiol sulfate (E2S) to estrone sulfate (E1S) isolated from the media and methanol washes of late gestation chorion tissue was 3-4 times greater than for the day 45 tissue.
Assuntos
17-Hidroxiesteroide Desidrogenases/metabolismo , Córion/enzimologia , Prenhez/metabolismo , Sulfotransferases , Sulfurtransferases/metabolismo , Animais , Estradiol/análogos & derivados , Estradiol/metabolismo , Estrogênios Conjugados (USP)/metabolismo , Estrona/análogos & derivados , Estrona/metabolismo , Feminino , Cobaias , Técnicas In Vitro , Especificidade de Órgãos , GravidezRESUMO
The possible role of intrauterine estrogen sulfatase and steroid sulfatase around the time of parturition in the guinea pig was investigated. [3H]Estrone sulfate or [3H]pregnenolone sulfate was incubated with intrauterine tissues. Estrogen sulfatase was found in placenta, endometrium, decidua basalis, amnion and chorion. The presence of steroid sulfatase was established in endometrium and decidua basalis but not in placenta or the fetal membranes. Examination of activities in early (days 32-35), mid (days 44-46) and late (within 5 days of parturition) gestation revealed no significant change in estrogen sulfatase specific activity in decidua basalis. However, in chorion and endometrium this activity was seen to increase approx. 12-fold (P less than 0.001) and 2.8-fold (P less than 0.001), respectively, from early to late gestation. In placenta, estrogen sulfatase activity appeared to increase 2.4-fold (P less than 0.001) and in amnion it decreased 2.8-fold (P less than 0.002). Steroid sulfatase activity in decidua basalis did not change during gestation, while activity in endometrium was found to increase by a factor of 5.3 (P less than 0.001), from early to late gestation. The increases, both in estrogen sulfatase activity in chorion, endometrium and placenta and in steroid sulfatase activity in endometrium, occurred primarily within the final 3 weeks of gestation. In contrast, the decrease in estrogen sulfatase activity in amnion occurred principally between the fifth and sixth weeks of gestation. Analysis of radiolabelled metabolites indicated that estradiol and progesterone could be produced via estrogen sulfatase and steroid sulfatase activities in certain tissues. Subcellular fractionation of tissues revealed that the greatest specific activity and total activity, in all cases, was associated with the 105,000 g pellet. Significant activity was also detected in the 750 and 10,000 g pellets but not in the 105,000 g supernatant. Radioimmunoassay of endogenous estradiol-17 beta (estradiol) in chorion extracts revealed a 6.3-fold increase in the hormone from mid to late gestation. Estradiol levels in endometrium and myometrium did not appear to change during this time. It was concluded that increased estrogen sulfatase activity in guinea pig chorion in late gestation occurs along with elevated levels of the hormone estradiol which may be important for parturition in this species.
Assuntos
Arilsulfatases/metabolismo , Estradiol/metabolismo , Sulfatases/metabolismo , Útero/enzimologia , Animais , Centrifugação , Endométrio/enzimologia , Feminino , Cobaias , Especificidade de Órgãos , Gravidez , Progesterona/metabolismo , Radioimunoensaio , Solubilidade , Esteril-Sulfatase , Frações Subcelulares/enzimologiaRESUMO
An oestrogen sulphotransferase, active towards both oestrone and oestradiol, and of high specific activity, is present in cytosol prepared from adrenal glands of both sexes of English Shorthair and Hartley guinea pigs. The ovarian and testicular cytosolic activities of this enzyme are markedly low in comparison with the adrenal activity. The adrenal enzyme is distinct from an accompanying pregnenolone sulphotransferase as judged by f.p.l.c. gel filtration, chromatofocusing, and differences in activation brought about by the addition of thiol groups. The oestrogen sulphotransferase behaved as a 67 kDa protein on a Sephadex G100 column and as a 48 kDa protein on f.p.l.c. gel-filtration columns. Two forms of the enzyme with apparent pI values of 6.1 and 5.5 were eluted during f.p.l.c. chromatofocusing. Sequential salt fractionation, f.p.l.c. gel filtration and elution from an agarose-hexane-adenosine-3',5'-diphosphate affinity gel has resulted in a preparation which, when resubmitted to f.p.l.c. gel filtration, yields a considerably purified oestrogen sulphotransferase. When submitted to SDS/polyacrylamide-gel electrophoresis under reducing conditions, a main protein band of 34-36 kDa is observed. It is suggested that the enzyme may exist as a dimer in the cytosol.
Assuntos
Glândulas Suprarrenais/enzimologia , Estrogênios/metabolismo , Pregnenolona/metabolismo , Sulfotransferases , Sulfurtransferases/metabolismo , Animais , Feminino , Cobaias , Masculino , Especificidade da Espécie , Especificidade por Substrato , Sulfurtransferases/isolamento & purificaçãoRESUMO
Subcellular fractions of purified pig Leydig cells from 7 different animals have been investigated with respect to their abilities to catalyze the sulfation of several steroids and the hydrolysis of the sulfated forms of these same steroids. Considerable estrone sulfate sulfohydrolase of pH optimum 7.5 and high apparent Km was found to be concentrated in the 105,000 g pellet but no evidence was obtained, in any subcellular fraction, for the presence of any activity toward the 3-sulfate of pregnenolone, dehydroepiandrosterone (DHA) or delta 5-androstene-3 beta,17 beta-diol (androstenediol). Cytosolic sulfotransferase activity toward estrone, pregnenolone, DHA and androstenediol was present in each animal. The activity toward these 4 substrates was eluted from a gel filtration column as a single peak of apparent molecular weight 43 KDa. Upon chromatofocusing, a sharp estrogen sulfotransferase peak of apparent pI 6.1 and pH optimum 9.5, was clearly separated from the neutral steroid sulfotransferase which eluted over a more acidic pH range in a manner suggestive of the presence of several isozymes. This latter, which exhibited a wide pH optimum range between 6 and 8.5, was most active toward androstenediol, and least active toward pregnenolone. The estrogen sulfotransferase exhibited Michaelis-Menten kinetics (apparent Km = 4 microM). The neutral steroid sulfotransferase activity increased in velocity with increasing androstenediol or DHA concentration up to 1 microM beyond which considerable substrate inhibition occurred. It appears from these data that neutral steroid sulfates synthesized in the pig Leydig cell are not subject to enzymic desulfation in the same cells.
Assuntos
Arilsulfatases/metabolismo , Células Intersticiais do Testículo/enzimologia , Sulfatases/metabolismo , Sulfotransferases , Sulfurtransferases/metabolismo , Androstenodióis/metabolismo , Animais , Cromatografia , Cromatografia em Gel , Citosol/enzimologia , Desidroepiandrosterona/metabolismo , Estrona/metabolismo , Concentração de Íons de Hidrogênio , Ponto Isoelétrico , Isoenzimas/metabolismo , Cinética , Masculino , Peso Molecular , Pregnenolona/metabolismo , Esteril-Sulfatase , SuínosRESUMO
The presence of two forms of estrogen sulfotransferase (EST) in 105,000 g cytosols of guinea pig chorion and liver has been established by chromatofocusing via a fast protein liquid chromatographic (FPLC) procedure. The chorion EST forms were eluted at pH 6.2 and 5.4, and the liver forms at 6.1 and 5.3. Each has been further purified by an affinity column step using Agarose-hexane-adenosine-3',5'-diphosphate (PAP-Agarose) gel to achieve up to 386-fold and 77-fold specific activity (SA) increases over cytosol for chorion and liver, respectively. The most highly purified preparations were extremely unstable unless protected by the addition of serum albumin of high purity. Each EST form exhibited an estimated molecular weight of 48-52 KDa by FPLC gel filtration and each acted upon both estrone (E1) and estradiol (E2). Each of these steroids inhibited sulfation of the other. A departure from Michaelis-Menten kinetics occurred, particularly in the case of chorion EST, at steroid substrate concentrations above 0.1-0.15 microM. E2 caused strong substrate inhibition of the most highly purified chorion EST. Chorion EST possessed considerable affinity for E1 and E2.
Assuntos
Córion/enzimologia , Isoenzimas/isolamento & purificação , Fígado/enzimologia , Sulfotransferases , Sulfurtransferases/isolamento & purificação , Animais , Cromatografia de Afinidade , Citosol/enzimologia , Feminino , Cobaias , Focalização Isoelétrica , Gravidez , Especificidade por SubstratoRESUMO
Mouse placental estrogen sulfotransferase (ST) was partially purified by fast protein liquid chromatography (FPLC) gel filtration in combination with FPLC anion exchange. Owing to the highly unstable nature of the enzyme, large increases in specific activity were not obtained. Storage of the ST in the presence of thiol groups at -20 degrees C stabilized the enzyme considerably. Forty-three percent of the cytosolic ST was bound to an Affi-Gel blue column and eluted as a broad peak at approximately 0.8 M NaCl. The use of the latter procedure, in combination with FPLC gel filtration, did not increase the specific activity substantially. Larger increases in specific activity were obtained using agarose-hexane-adenosine-3',5'-diphosphate affinity chromatography. The bound ST activity was eluted under a single peak at 1 mM ADP. Increases in specific activity following use of this column averaged 54-fold but could reach 90-fold. Attempts at further purification of this material resulted in low recovery and decreased specific activity. Velocity versus substrate concentration curves show that estrone and particularly estradiol inhibit the partially purified mouse placental sulfotransferase above 0.1-0.25 microM substrate concentrations.
Assuntos
Placenta/enzimologia , Sulfotransferases , Sulfurtransferases/isolamento & purificação , Animais , Cromatografia em Gel , Feminino , Camundongos , GravidezRESUMO
Some characteristics of estrogen sulfotransferases from guinea pig liver and chorion were compared. Liver cytosolic activity was stimulated 10-fold by 25 mM monothiolglycerol and 2-fold by 15 mM MgCl2 or CaCl2, similar to that found previously for chorion. Liver and chorion activities were each eluted as a single peak from fast protein liquid chromatography (FPLC) gel filtration columns at apparent molecular weights of 52,300 and 50,000, respectively. Each was eluted during FPLC anion exchange under single, wide peaks with low recoveries. Liver sulfotransferase activity was eluted from Affi-gel Blue columns in the form of several peaks whereas the chorion activity behaved as a single species. The enzymes from both tissues, when partially purified by gel filtration followed by anion exchange, acted upon estrone and estradiol at the 3-position but activity toward dehydroepiandrosterone and testosterone was minimal or undetectable. Affi-gel Blue chromatography followed by FPLC gel filtration resulted in increases in specific activity of 26- and 90-fold for liver and chorion, respectively. Both enzymes were eluted from agarose-hexane-adenosine 3',5'-diphosphate (PAP-agarose) columns as single peaks. Average increases in specific activity for this column step were 40-fold and 96-fold for the entire eluted peaks of liver and chorion enzyme, respectively. Individual fractions from the PAP-agarose column indicated a specific activity increase of as much as 60-fold for liver and 208-fold for chorion. These latter were markedly unstable and it was not possible to obtain further purification by additional steps. Velocity versus substrate concentration curves for the partially purified enzymes showed complex kinetics, particularly with estradiol as substrate.