Association of voltage-gated sodium channel mutations with field-evolved pyrethroid resistant phenotypes in soybean aphid and genetic markers for their detection.

The frequent use of insecticides to manage soybean aphids, Aphis glycines (Hemiptera: Aphididae), in the United States has contributed to field-evolved resistance. Pyrethroid-resistant aphids have nonsynonymous mutations in the voltage-gated sodium channel (vgsc). We identified a leucine to phenylalanine mutation at position 1014 (L1014F) and a methionine to isoleucine mutation (M918I) of the A. glycines vgsc, both suspected of conferring knockdown resistance (kdr) to lambda-cyhalothrin. We developed molecular markers to identify these mutations in insecticide-resistant aphids. We determined that A. glycines which survived exposure to a diagnostic concentration of lambda-cyhalothrin and bifenthrin via glass-vial bioassays had these mutations, and showed significant changes in the resistance allele frequency between samples collected before and after field application of lambda-cyhalothrin. Thus, a strong association was revealed between aphids with L1014F and M918I vgsc mutations and survival following exposure to pyrethroids. Specifically, the highest survival was observed for aphids with the kdr (L1014F) and heterozygote super-kdr (L1014F + M918I) genotypes following laboratory bioassays and in-field application of lambda-cyhalothrin. These genetic markers could be used as a diagnostic tool for detecting insecticide-resistant A. glycines and monitoring the geographic distribution of pyrethroid resistance. We discuss how generating these types of data could improve our efforts to mitigate the effects of pyrethroid resistance on crop production.

Lack of Evidence for Fitness Costs in Soybean Aphid (Hemiptera: Aphididae) With Resistance to Pyrethroid Insecticides in the Upper Midwest Region of the United States.

Twenty years after the arrival of soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), in the United States, it remains the most economically important arthropod pest of soybean in the Upper Midwest Region. After years of repeated and sustained insecticidal pressures placed on the aphid, resistance to the pyrethroid class of insecticides has been documented in multiple years over a large geographic area. In this study, the fitness of aphid isolates displaying resistant and susceptible phenotypes to λ-cyhalothrin were compared within several experiments over three soybean-growing seasons. Rates of population increase were evaluated on whole plants in the greenhouse, intrinsic rates of increase were calculated from leaf discs in the laboratory, and aphid size and asymmetry were compared through tibial measurements. No evidence of a fitness cost associated with the resistant phenotype was seen in any of our experiments. In contrast, individual resistant isolates occasionally supported significantly higher fitness values than some susceptible isolates. Additionally, a pooled analysis comparing resistant and susceptible phenotypes across years and isolates revealed that, on average, the resistant phenotype had significantly higher fitness values than the susceptible phenotype in most experiments. The lack of reproductive fitness costs associated with the pyrethroid-resistant phenotype raises concerns for longevity of pyrethroid use in soybean aphid management.

Linear Support Vector Machine Classification of Plant Stress From Soybean Aphid (Hemiptera: Aphididae) Using Hyperspectral Reflectance.

Spectral remote sensing has the potential to improve scouting and management of soybean aphid (Aphis glycines Matsumura), which can cause yield losses of over 40% in the North Central Region of the United States. We used linear support vector machines (SVMs) to determine 1) whether hyperspectral samples could be classified into treat/no-treat classes based on the economic threshold (250 aphids per plant) and 2) how many wavelengths or features are needed to generate an accurate model without overfitting the data. A range of aphid infestation levels on soybean was created using caged field plots in 2013, 2014, 2017, and 2018 in Minnesota and in 2017 and 2018 in Iowa. Hyperspectral measurements of soybean canopies in each plot were recorded with a spectroradiometer. SVM training and testing were performed using 15 combinations of normalized canopy reflectance at wavelengths of 720, 750, 780, and 1,010 nm. Pairwise Bonferroni-adjusted t-tests of Cohen's kappa values showed four wavelength combinations were optimal, namely model 1 (780 nm), model 2 (780 and 1,010 nm), model 3 (780, 1,010, and 720 nm), and model 4 (780, 1,010, 720, and 750 nm). Model 2 showed the best overall performance, with an accuracy of 89.4%, a sensitivity of 81.2%, and a specificity of 91.6%. The findings from this experiment provide the first documentation of successful classification of remotely sensed spectral data of soybean aphid-induced stress into threshold-based classes.

Developing an Injury Severity to Yield Loss Relationship for Soybean Gall Midge (Diptera: Cecidomyiidae).

Soybean gall midge, Resseliella maxima Gagné (Diptera: Cecidomyiidae), is a newly identified pest confirmed on soybean, Glycine max (L.) Merr. (Fabales: Fabaceae). To date, soybean gall midge has been found in Nebraska, Iowa, South Dakota, Minnesota, and Missouri, and has caused severe economic loss to commercial fields since 2018. Much is still unknown about this pest, so research efforts have been focused on biology and management. Larvae feed on the inside of the stem just above the soil line and are difficult to access and time-consuming to sample. In order to accelerate nondestructive sampling efforts, we developed an injury rating system to quantify the severity of plant injury from soybean gall midge larvae. Research plots from 2019 and 2020 in Iowa and Nebraska were evaluated for injury throughout the growing season and yield was measured. Our objective was to describe the relationship between injury severity and yield loss caused from soybean gall midge. A nonlinear regression model was developed to validate our injury rating system and to express the relationship between season long injury severity and yield loss. Results from our analysis indicate the injury rating system we developed correlates well with yield loss caused by larvae and may be an important tool for understanding the economic impact of this emergent pest of soybeans.

Evidence of enhanced reproductive performance and lack-of-fitness costs among soybean aphids, Aphis glycines, with varying levels of pyrethroid resistance.

BACKGROUND: Foliar application of insecticides is the main strategy to manage soybean aphid, Aphis glycines (Hemiptera: Aphididae), in the northcentral United States. Subpopulations of A. glycines have multiple nonsynonymous mutations in the voltage-gated sodium channel (vgsc) genes that are associated with pyrethroid resistance. We explored if fitness costs are associated with phenotypes conferred by vgsc mutations using life table analyses. We predicted that there would be significant differences between pyrethroid susceptibility and field-collected, parthenogenetic isofemale clones with differing, nonsynonymous mutations in vgsc genes. RESULTS: Estimated resistance ratios for the pyrethroid-resistant clones ranged from 3.1 to 37.58 and 5.6 to 53.91 for lambda-cyhalothrin and bifenthrin, respectively. Although life table analyses revealed some biological and demographic parameters to be significantly different among the clonal lines, there was no association between levels of pyrethroid resistance and a decline in fitness. By contrast, one of the most resistant clonal lines (SBA-MN1-2017) had a significantly higher finite rate of increase, intrinsic rate of increase and greater overall fitness compared to the susceptible control and other pyrethroid-resistant clonal lines. CONCLUSIONS: Our life history analysis suggests that there are no negative pleotropic effects associated with the pyrethroid resistance in the clonal A. glycines lines used in this study. We discuss the potential impact of these results on efficacies of insecticide resistance management (IRM) and integrated pest management (IPM) plans directed at delaying the spread of pyrethroid-resistant A. glycines.

Developing a decision-making framework for insect pest management: a case study using Aphis glycines (Hemiptera: Aphididae).

BACKGROUND: The profitability of farming varies based on factors such as a crop's market value, input costs and occurrence of resistant pests, all capable of altering the value of pest management tactics in an integrated pest management program. We provide a framework for calculating expected yield and expected net revenue of pest management scenarios, using the soybean aphid (Aphis glycines) as a case study. Foliar insecticide and host-plant resistance are effective management tactics for preventing yield loss from soybean aphid outbreaks; however, pyrethroid-resistant aphid populations pose a management challenge for farmers. We evaluated eight scenarios relevant to soybean aphid management in Iowa with varying probabilities of aphid outbreaks and insecticide-resistant aphids occurring. RESULTS: Our equation suggests that insecticide use is profitable when the probability of an aphid outbreak is ≥29%, and soybean production will become more costly with increasing probability of pyrethroid-resistant aphids. If farmers continue to use pyrethroids, they will not experience financial consequences from pyrethroid-resistant aphids until the chance of insecticide resistance is 48%. Aphid-resistant varieties provided consistent yield and offered the highest net revenue under all conditions. CONCLUSION: This framework can be used for other crop-pest systems to evaluate the profitability of management tactics and investigate how resistance impacts revenue for farmers. Including the cost of resistance in crop budgets can help farmers and agronomic consultants comprehend these impacts and enhance decision-making to increase revenue and curb resistance development.

Genome scan detection of selective sweeps among biotypes of the soybean aphid, Aphis glycines, with differing virulence to resistance to A. glycines (Rag) traits in soybean, Glycine max.

Multiple biotypes of soybean aphid, Aphis glycines, occur in North America adapted for survival (virulence) on soybean, Glycine max, with one or more different resistance to A. glycines (Rag) traits. The degree of genome-wide variance between biotypes and the basis of virulence remains unknown, but the latter is hypothesized to involve secreted effector proteins. Between 167,249 and 217,750 single nucleotide polymorphisms (SNPs) were predicted from whole genome re-sequencing of A. glycines avirulent biotype 1 (B1) and virulent B2, B3 and B4 colony-derived iso-female lines when compared to the draft B1 genome assembly, Ag_bt1_v6.0. Differences in nucleotide diversity indices (π) estimated within 1000 bp sliding windows demonstrated that 226 of 353 (64.0%) regions most differentiated between B1 and ≥ 2 virulent biotypes, representing < 0.1% of the 308 Mb assembled genome size, are located on 15 unordered scaffolds. Furthermore, these 226 intervals were coincident and show a significant association with 326 of 508 SNPs with significant locus-by-locus FST estimates between biotype populations (r = 0.6271; F1,70 = 45.36, P < 0.001) and genes showing evidence of directions selection (πN/πS > 2.0; r = 0.6233; F1,70 = 50.20, P < 0.001). A putative secreted effector glycoprotein is encoded in proximity to genome intervals of low estimated π (putative selective sweep) within avirulent B1 compared to all three virulent biotypes. Additionally, SNPs are clustered in or in proximity to genes putatively involved in intracellular protein cargo transport and the regulation of secretion. Results of this study indicate that factors on a small number of scaffolds of the A. glycines genome may contribute to variance in virulence towards Rag traits in G. max.

Implementation of a Diagnostic-Concentration Bioassay for Detection of Susceptibility to Pyrethroids in Soybean Aphid (Hemiptera: Aphididae).

Soybean aphid, Aphis glycines Matsumura, remains the most economically damaging arthropod pest of soybean in the midwestern United States and southern Canada. Foliar applications of a limited number of insecticide modes of action have been the primary management tactic, and pyrethroid resistance was documented recently with full concentration-response leaf-dip and glass-vial bioassays. Full concentration-response bioassays can be cumbersome, and a more efficient assessment tool was needed. In this study, we implemented a diagnostic-concentration glass-vial bioassay using bifenthrin and λ-cyhalothrin. Bioassays were conducted with field-collected soybean aphid populations to assess the geographic extent and severity of resistance to pyrethroids. In 2017, 10 of 18 and 11 of 21 field populations tested with bifenthrin and λ-cyhalothrin, respectively, had mean proportion mortalities less than the susceptible laboratory population. In 2018, 17 of 23 and 13 of 23 field populations tested with bifenthrin and λ-cyhalothrin, respectively, had mean proportion mortalities less than the susceptible laboratory population. Populations collected after reported field failures of a pyrethroid insecticide generally had mean proportion mortalities less than the susceptible laboratory population. In both years, there was a strong correlation between chemistries, which suggests cross-resistance between these insecticides. The diagnostic-concentration glass-vial bioassays reported here will provide the foundation for an insecticide resistance monitoring program with the ability to determine practical levels and geographic extent of insecticide resistance.

Efficacy of afidopyropen against soybean aphid (Hemiptera: Aphididae) and toxicity to natural enemies.

BACKGROUND: Soybean aphid, Aphis glycines (Hemiptera: Aphididae), remains the most significant soybean insect pest in the North Central Region of the USA. The sustainability of reliance on only a few insecticide groups for this pest is questionable. We evaluate afidopyropen, a novel pyropene insecticide (Group 9D), for efficacy against A. glycines in field and greenhouse experiments and toxicity to common natural enemies in laboratory experiments. RESULTS: Across 4 site-years of field experiments and a greenhouse experiment, afidopyropen reduced A. glycines populations similar to commonly used broad-spectrum [i.e. lambda-cyhalothrin (Group 3A) and chlorpyrifos (Group 1B)] insecticides and potential selective insecticides [i.e. sulfoxaflor (Group 4C) and flupyradifurone (Group 4D)]. In the greenhouse, however, A. glycines mortality was delayed slightly for afidopyropen compared to the other insecticides. In laboratory experiments with natural enemies of A. glycines, afidopyropen was not toxic to adult or third instar Hippodamia convergens (Coleoptera: Coccinellidae) or adult Orius insidiosus (Hemiptera: Anthocoridae), and was only moderately toxic to Aphelinus certus (Hymenoptera: Aphelinidae). CONCLUSION: Afidopyropen is effective against A. glycines and relatively non-toxic to natural enemies, and appears to be an effective option for integrated pest management and insecticide resistance management programs for A. glycines. © 2019 Society of Chemical Industry.

Soybean aphid (Hemiptera: Aphididae) response to lambda-cyhalothrin varies with its virulence status to aphid-resistant soybean.

BACKGROUND: Soybean aphid, Aphis glycines, is an invasive insect in North America, considered one of the most important pests of soybean. Their management relies heavily on foliar insecticides, but there is growing effort to expand these tools to include aphid-resistant varieties. We explored if the LC50 and LC25 of lambda-cyhalothrin varied between virulent (resistant to Aphis glycines (Rag) soybeans) and avirulent (susceptible to Rag-genes soybeans) populations of soybean aphid with a leaf-dip bioassay. We also investigated the response to the LC25 of lambda-cyhalothrin on adults (F0) and their progeny (F1) for both avirulent and virulent soybean aphid. RESULTS: The LC50 of the virulent aphid population was significantly higher compared with the LC50 of the avirulent population. The LC25 significantly reduced fecundity of the F0 generation of avirulent soybean aphid, but no significant effect was observed for virulent aphids. In addition, the LC25 significantly shortened the adult pre-oviposition period (APOP) and lengthened the total pre-oviposition period (TPOP) of avirulent aphids, while the mean generation time (T) was significantly increased. For the virulent aphid, sublethal exposure significantly lengthened development time of first and third instars, TPOP, and adult longevity. In addition, all demographic parameters of virulent soybean aphid were significantly affected when they were exposed to the LC25 of lambda-cyhalothrin. CONCLUSION: Our results demonstrate lambda-cyhalothrin is less toxic to virulent aphids and exposure to the LC25 can trigger hormesis, which may have implications for the long-term management of this pest with this insecticide as well as with aphid-resistant varieties of soybean. © 2019 Society of Chemical Industry.

Detection of Stress Induced by Soybean Aphid (Hemiptera: Aphididae) Using Multispectral Imagery from Unmanned Aerial Vehicles.

Soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is a common pest of soybean, Glycine max (L.) Merrill (Fabales: Fabaceae), in North America requiring frequent scouting as part of an integrated pest management plan. Current scouting methods are time consuming and provide incomplete coverage of soybean. Unmanned aerial vehicles (UAVs) are capable of collecting high-resolution imagery that offer more detailed coverage in agricultural fields than traditional scouting methods. Recently, it was documented that changes to the spectral reflectance of soybean canopies caused by aphid-induced stress could be detected from ground-based sensors; however, it remained unknown whether these changes could also be detected from UAV-based sensors. Small-plot trials were conducted in 2017 and 2018 where cages were used to manipulate aphid populations. Additional open-field trials were conducted in 2018 where insecticides were used to create a gradient of aphid pressure. Whole-plant soybean aphid densities were recorded along with UAV-based multispectral imagery. Simple linear regressions were used to determine whether UAV-based multispectral reflectance was associated with aphid populations. Our findings indicate that near-infrared reflectance decreased with increasing soybean aphid populations in caged trials when cumulative aphid days surpassed the economic injury level, and in open-field trials when soybean aphid populations were above the economic threshold. These findings provide the first documentation of soybean aphid-induced stress being detected from UAV-based multispectral imagery and advance the use of UAVs for remote scouting of soybean aphid and other field crop pests.

Evaluating Soybean Aphid-Resistant Varieties in Different Environments to Estimate Financial Outcomes.

Farmers face many choices when selecting seed for soybean (Glycine max (L.) Merr.) production, including highly desired herbicide tolerance traits. Despite the convenience of herbicide tolerance, resistant weeds and technology fees may reduce utility and profitability of these varieties, especially when commodity prices are low. Sporadic outbreaks of soybean aphid (Aphis glycines Matsumura, Hemiptera: Aphididae) that require insecticide use for optimal yield can be a further complication for farmers in Iowa. Soybean aphid-resistant varieties are commercially available, but in limited genetic backgrounds without herbicide tolerance. We hypothesized yield and value of resistance traits will vary based on the environment. We established plots at two locations with different risks of soybean aphid outbreaks and used two planting dates at each location to mimic different yield environments. In 2016 and 2017, we planted four varieties that varied in their susceptibility to soybean aphids and glyphosate, and applied insecticides if aphid populations reached an economic threshold. Regardless of genetic background, aphid-resistant varieties prevented populations from reaching the economic threshold at all environments. We observed no significant difference in yield between resistant and susceptible varieties, revealing this trait is as effective at protecting yield as an insecticide application on susceptible varieties at the high-risk location. We also explored the value of each variety in different environments. Resistant varieties produced greater potential net revenue than susceptible varieties at the high-risk location, while the opposite occurred at the low-risk location. Resistant varieties with herbicide tolerance, if made available, would be the most valuable across all environments.

Effects of endophytic entomopathogenic fungi on soybean aphid and identification of Metarhizium isolates from agricultural fields.

Terrestrial plants can harbor endophytic fungi that may induce changes in plant physiology that in turn affect interactions with herbivorous insects. We evaluated whether the application of entomopathogenic fungi Beauveria bassiana and Metarhizium brunneum to soybean seeds could become endophytic and affect interactions with soybean aphid (Aphis glycines Matsumura). It was found that A. glycines population sizes increased on plants with M. brunneum (strain F52) seed inoculum, but no significant effects were shown with analogous treatments with B. bassiana (strain GHA). Fungi recovered from soybean plant tissues indicate that endophytism was established, and that B. bassiana was more prevalent. Metarhizium brunneum was only recovered from stems, but B. bassiana was recovered from stems and leaves. This work confirms that some entomopathogenic fungi can be endophytic in soybean, however, some of these fungi may have a negative effect on the plants by increasing susceptibility of soybean to A. glycines. We also used DNA sequence data to identify species of Metarhizium obtained from agricultural fields in Iowa. Phylogenetic analyses, based on DNA sequence data, found that all isolates were Metarhizium robertsii, which is consistent with past studies indicating a cosmopolitan distribution and wide host range for this species. These results are important for understanding the dynamics of implementing environmentally sustainable measures for the control of pest insects.

Interactions of effects of host plant resistance and seed treatments on soybean aphid (Aphis glycines Matsumura) and soybean cyst nematode (Heterodera glycines Ichinohe).

BACKGROUND: The soybean cyst nematode, Heterodera glycines, and soybean aphid, Aphis glycines, are invasive, widespread and economically important pests of soybean, Glycine max, in North America. Management of these pests relies primarily on use of pesticides and soybean germplasm with genetic resistance. A 3-year field study and complementary greenhouse experiment were conducted to determine the benefits of host plant resistance (HPR) and pesticidal seed treatments for managing pest populations and preserving soybean yield. RESULTS: Host plant resistance significantly decreased the abundance of A. glycines and, in most study sites, suppressed H. glycines. Neonicotinoid seed treatment reduced A. glycines abundance on the cultivar that was susceptible to both aphids and nematodes, but abamectin nematicide seed treatment had no effect on H. glycines populations in the field or greenhouse. CONCLUSION: These results suggest that the seed treatments included in our experiments may suppress pests, but not consistently for all soybean cultivars or study sites. Ultimately, HPR more consistently reduced pest numbers compared with the use of pesticidal seed treatments. The planting of HPR cultivars should be a primary tool for integrated pest management of both soybean pests. © 2017 Society of Chemical Industry.

Evidence for Soybean Aphid (Hemiptera: Aphididae) Resistance to Pyrethroid Insecticides in the Upper Midwestern United States.

Soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is a damaging invasive pest of soybean in the upper Midwest. Threshold-based insecticide applications are the primary control method for soybean aphid, but few insecticide groups are available (i.e., pyrethroids, organophosphates, and neonicotinoids). To quantify current levels of soybean aphid susceptibility to pyrethroids in the upper Midwest and monitor for insecticide resistance, leaf-dip bioassays were performed with λ-cyhalothrin in 2013-2015, and glass-vial bioassays were performed with λ-cyhalothrin and bifenthrin in 2015 and 2016. Soybean aphids were collected from 27 population-years in Minnesota and northern Iowa, and were compared with a susceptible laboratory colony with no known insecticide exposure since discovery of soybean aphid in North America in 2000. Field-collected aphids from some locations in leaf-dip and glass-vial bioassays had significantly lower rates of insecticide-induced mortality compared with the laboratory population, although field population susceptibility varied by year. In response to sublethal concentrations of λ-cyhalothrin, adult aphids from some locations required higher concentrations of insecticide to reduce nymph production compared with the laboratory population. The most resistant field population demonstrated 39-fold decreased mortality compared with the laboratory population. The resistance documented in this study, although relatively low for most field populations, indicates that there has been repeated selection pressure for pyrethroid resistance in some soybean aphid populations. Integrated pest management and insecticide resistance management should be practiced to slow further development of soybean aphid resistance to pyrethroids.

Assessing the value and pest management window provided by neonicotinoid seed treatments for management of soybean aphid (Aphis glycines Matsumura) in the Upper Midwestern United States.

BACKGROUND: A 2-year, multi-state study was conducted to assess the benefits of using soybean seed treated with the neonicotinoid thiamethoxam to manage soybean aphid in the upper Midwestern USA and compare this approach with an integrated pest management (IPM) approach that included monitoring soybean aphids and treating with foliar-applied insecticide only when the economic threshold was reached. Concentrations of thiamethoxam in soybean foliage were also quantified throughout the growing season to estimate the pest management window afforded by insecticidal seed treatments. RESULTS: Both the IPM treatment and thiamethoxam-treated seed resulted in significant reductions in cumulative aphid days when soybean aphid populations reached threshold levels. However, only the IPM treatment resulted in significant yield increases. Analysis of soybean foliage from thiamethoxam-treated seeds indicated that tissue concentrations of thiamethoxam were statistically similar to plants grown from untreated seeds beginning at the V2 growth stage, indicating that the period of pest suppression for soybean aphid is likely to be relatively short. CONCLUSION: These data demonstrate that an IPM approach, combining scouting and foliar-applied insecticide where necessary, remains the best option for treatment of soybean aphids, both in terms of protecting the yield potential of the crop and of break-even probability for producers. Furthermore, we found that thiamethoxam concentrations in foliage are unlikely to effectively manage soybean aphids for most of the pests' activity period across the region. © 2017 Society of Chemical Industry.

Abundance of Soil-Borne Entomopathogenic Fungi in Organic and Conventional Fields in the Midwestern USA with an Emphasis on the Effect of Herbicides and Fungicides on Fungal Persistence.

Entomopathogenic fungi (EPF) are widespread in agricultural fields and help suppress crop pests. These natural enemies may be hindered by certain agronomic practices associated with conventional agriculture including the use of pesticides. We tested whether the abundance of EPF differed between organic and conventional fields, and whether specific cropping practices and soil properties were correlated with their abundance. In one year of the survey, soil from organic fields and accompanying margins had significantly more EPF than conventional fields and accompanying margins. Regression analysis revealed that the percentage of silt and the application of organic fertilizer were positively correlated with EPF abundance; but nitrogen concentration, tillage, conventional fields, and margins of conventional fields were negatively correlated with EPF abundance. A greenhouse experiment in which fungicides and herbicides were applied to the soil surface showed no significant effect on EPF. Though organic fields were perceived to be more suitable environments for EPF, abiotic factors and cropping practices such as tillage may have greater impacts on the abundance of EPF. Also, fungicides and herbicides may not be as toxic to soil-borne EPF as originally thought.

Effects of Rag1 on the preference and performance of soybean defoliators.

The Rag1 gene confers antibiotic resistance to soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), and in 2010, varieties expressing Rag1 were released for commercial use in the United States. We do not know how Rag1 varieties will influence the broader community of defoliating insects that inhabit soybean fields. In 2010 and 2011, the preference and performance of pest insects that defoliate soybeans [Glycines max (L.) Merr] were tested using Rag1 and aphid-susceptible varieties. Three coleopterans and four lepidopterans were used: northern corn rootworm, Diabrotica barberi Smith & Lawrence (Coleoptera: Chrysomelidae); southern corn rootworm, Diabrotica undecimpunctata howardi Barber (Coleoptera: Chrysomelidae); bean leaf beetle, Ceratoma trsifurcata Förster (Coleoptera: Chrysomelidae); fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae); corn earworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae); soybean looper, Chrysodeix includens (Walker) (Lepidoptera: Noctuidae); and velvet-bean caterpillar, Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae). The preference of insects was evaluated in choice and no-choice tests using Rag1 and susceptible soybeans. Lepidopterans also were evaluated on Rag1 leaves using four nutritional indices: relative growth rate, approximate digestibility, and efficiency of conversion of ingested material. In the majority of preference tests, no effect of Rag1 was detected, and in cases where preferences were found, there was no consistent pattern of preference for Rag1 vs. susceptible leaf tissue. Helicoverpa zea demonstrated a preference for resistant leaf tissue, but this was dependent on the genetic background of the variety. Evaluations of nutritional indices indicated that three species of Lepidoptera, S. frugiperda, H. zea, and A. gemmatalis, displayed reduced conversion efficiency for Rag1 soybeans, suggesting effects of antibiosis.

Effects of the insect growth regulator, novaluron on immature alfalfa leafcutting bees, Megachile rotundata.

Alfalfa leafcutting bees, Megachile rotundata F. (Hymenoptera: Megachilidae), are the most common pollinators of alfalfa in the Pacific Northwest. Reports from users of M. rotundata in Idaho, Utah and Colorado have indicated exceptionally poor bee return from fields treated with novaluron to control Lygus spp. Our goal was to evaluate novaluron toxicity to immature M. rotundata using two different possible mechanisms of exposure. One goal was to assess immature mortality via treating nectar-pollen provisions and adults with novaluron. Immature M. rotundata mortality in all novaluron provision dosing treatments was significantly higher than the water or blank controls, providing evidence that novaluron is toxic to progeny in nest cells. The mean cumulative frequency showed that more eggs and 1st-2nd instars died compared to older instars. Female M. rotundata nested similarly in field cages during the field cage experiment; however, there was greater immature mortality in cages where females were fed sugar-water + novaluron compared to sugar-water only. Although females provided adequate provisions, there was a low percentage of egg hatch and larval development when females ingested novaluron before mating and nesting. Novaluron was also present in egg provision of bees collecting resources from novaluron-sprayed plants. At least 84% of progeny died when the females were allowed to mate and nest 24 hours after a novaluron application. Novaluron could be contributing to poor bee return in alfalfa grown for seed. Timely insecticide applications to suppress Lygus spp. is an important consideration to improve ongoing bee health.

Probability of cost-effective management of soybean aphid (Hemiptera: Aphididae) in North America.

Soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is one of the most damaging pests of soybean, Glycine max (L.) Merrill, in the midwestern United States and Canada. We compared three soybean aphid management techniques in three midwestern states (Iowa, Michigan, and Minnesota) for a 3-yr period (2005-2007). Management techniques included an untreated control, an insecticidal seed treatment, an insecticide fungicide tank-mix applied at flowering (i.e., a prophylactic treatment), and an integrated pest management (IPM) treatment (i.e., an insecticide applied based on a weekly scouting and an economic threshold). In 2005 and 2007, multiple locations experienced aphid population levels that exceeded the economic threshold, resulting in the application of the IPM treatment. Regardless of the timing of the application, all insecticide treatments reduced aphid populations compared with the untreated, and all treatments protected yield as compared with the untreated. Treatment efficacy and cost data were combined to compute the probability of a positive economic return. The IPM treatment had the highest probability of cost effectiveness, compared with the prophylactic tank-mix of fungicide and insecticide. The probability of surpassing the gain threshold was highest in the IPM treatment, regardless of the scouting cost assigned to the treatment (ranging from $0.00 to $19.76/ha). Our study further confirms that a single insecticide application can enhance the profitability of soybean production at risk of a soybean aphid outbreak if used within an IPM based system.