Tea (Camellia sinensis) cultivated in three agro-ecological regions of Bangladesh: Unveiling the variability of methylxanthine, bioactive phenolic compound, and antioxidant activity.

Tea (Camellia sinensis) is a widely consumed beverage known for its numerous health benefits, largely attributed to its rich content of quality determining secondary metabolites such as methylxanthine compounds and bioactive phenolic compounds. The goal of this study was to find out variations of the levels of methylxanthines, bioactive phenolic compounds, and antioxidant activity in methanolic and hot water extracts of 129 tea samples grown in three different ecological regions of Bangladesh named Panchagar, Sylhet, and Chattogram. Methylxanthine and bioactive phenolic compounds were determined by using HPLC-DAD, and the antioxidant profile was analysed by UV-vis spectrophotometric methods for methanol and hot water extracts of tea leaves. The IC50 values showed the trend as Panchagar > Sylhet > Chattogram and Sylhet > Chattogram > Panchagar for water and methanol extract, respectively. The results revealed significant (p < 0.05) variations in the levels of methylxanthines content: Panchagar > Chattogram > Sylhet. Caffeine was significantly higher (103.02 ± 5.55 mg/g of dry extract) in the methanolic extract of tea leaves of Panchagar district and lower (53.33 ± 4.30 mg/g of dry extract) in the hot water extract of Sylhet district. Panchagar and Chattogram possessed significantly (p < 0.05) higher catechin content for methanol (57.01 ± 5.50 mg/g dry extract) and hot water (55.23 ± 4.11 mg/g dry extract) extracts, respectively. The utilization of canonical discriminant functions yielded highly favorable outcomes in the classification of tea from three distinct cultivation origins in Bangladesh, relying on their inherent features. This study demonstrated the potential effects of geographical variations on the bioactive compounds and antioxidant properties of tea, emphasizing the importance of regional differences in tea cultivation for optimizing its health benefits as well as dispersing tea cultivation across the country.

Obesity Differs from Diabetes Mellitus in Antibody and T Cell Responses Post COVID-19 Recovery.

Obesity and type 2 diabetes (DM) are risk factors for severe COVID-19 outcomes, which disproportionately affect South Asian populations. This study aims to investigate the humoral and cellular immune responses to SARS-CoV-2 in adult COVID-19 survivors with obesity and DM in Bangladesh. In this cross-sectional study, SARS-CoV-2-specific antibody and T cell responses were investigated in 63 healthy and 75 PCR-confirmed COVID-19 recovered individuals in Bangladesh, during the pre-vaccination first wave of the COVID-19 pandemic in 2020. In COVID-19 survivors, SARS-CoV-2 infection induced robust antibody and T cell responses, which correlated with disease severity. After adjusting for age, sex, DM status, disease severity, and time since onset of symptoms, obesity was associated with decreased neutralising antibody titers, and increased SARS-CoV-2 spike-specific IFN-γ response along with increased proliferation and IL-2 production by CD8+ T cells. In contrast, DM was not associated with SARS-CoV-2-specific antibody and T cell responses after adjustment for obesity and other confounders. Obesity is associated with lower neutralising antibody levels and higher T cell responses to SARS-CoV-2 post COVID-19 recovery, while antibody or T cell responses remain unaltered in DM.

Development of Novel Foxtail Millet-Based Nutri-Rich Instant Noodles: Chemical and Quality Characteristics.

Noodles are a popular snack mainly produced from wheat flour; however, the low contents of protein, minerals, and lysine are a concern. Therefore, this research developed nutri-rich instant noodles by using foxtail millet (FTM) (Setaria italic) flour to improve the contents of protein and nutrients and increase its commercial importance. FTM flour was mixed with wheat flour (Triticum aestivum) at a ratio of 0:100, 30:60, 40:50, and 50:40, and the samples were named as control, FTM30, FTM40, and FTM50 noodles, respectively. Mushroom (Pleurotus ostreatus) and rice bran (Oryza sativa L.) flour were added at a percentage of 5% to all the composite noodles (FTM30, FTM40, and FTM50 noodles). The contents of biochemicals, minerals, and amino acids, as well as the organoleptic properties of the noodles, were examined and compared with wheat flour as a control. The results revealed that the carbohydrate (CHO) content of FTM50 noodles was significantly lower (p < 0.05) than all the developed and five commercial noodles named A-1, A-2, A-3, A-4, and A-5. Moreover, the FTM noodles had significantly higher levels of protein, fiber, ash, calcium, and phosphorous than the control and commercial noodles. The percentage of lysine calculated protein efficiency ratio (PER), essential amino acid index (EAAI), biological value (BV), and chemical score (CS) of FTM50 noodles were also higher than that of the commercial noodles. The total bacterial count was nil for the FTM50 noodles, and the organoleptic properties were consistent with those of acceptable standards. The results could encourage the application of FTM flours for the development of variety and value-added noodles with enhanced level of nutrients.

Antimicrobial activity of peels and physicochemical properties of juice prepared from indigenous citrus fruits of Sylhet region, Bangladesh.

Scientists are indeed fascinated by the pharmacologically important chemicals found in medicinal plants. Citrus fruits possess several curing agents for the prevention of diseases. Therefore, experiments were carried out to test the antibacterial activity of methanolic extracts of peels from various locally available citrus fruits such as citron (Citrus medica), satkora (Citrus macroptera) and adajamir (Citrus assamensis) against Bacillus spp. and E. coli using the disc diffusion method. Different physicochemical characteristics of fruit juice were also determined. Methanolic extract of satkora peel had the highest antibacterial activity of 2.2 and 2.6 cm while adajamir had the lowest antibacterial activity of 1.7 and 2.1 cm as ZOI against Bacillus spp. and E. coli, respectively. In the case of pH and total soluble solids (TSS), the three citrus varieties showed a small variation where satkora showed the highest total phenolic content (TPC), vitamin C content, and antioxidant activity than the others. Citrus fruits can be exploited as antibacterial and antioxidant ingredients in food and nutraceuticals, according to the findings of this study.

Delayed presentation of congenital rectal stenosis associated with Down's syndrome and hypothyroidism: Case report.

Rectal stenosis is a rare variety of rectal atresia. A membrane separates the rectum from the anal canal in the presence of a normal anus. We report a case of rectal stenosis associated with Down's syndrome and hypothyroidism in whom rectal stenosis was diagnosed at the age of 17 years.

Management of Acute Appendicitis in Children During COVID-19 and Perspectives of Pediatric Surgeons From South Asia: Survey Study.

BACKGROUND: Nonoperative treatment (NOT) of pediatric appendicitis as opposed to surgery elicits great debate and is potentially influenced by physician preferences. Owing to the effects of the COVID-19 pandemic on health care, the practice of NOT has generally increased by necessity and may, in a post-COVID-19 world, change surgeons' perceptions of NOT. OBJECTIVE: The aim of this study was to determine whether the use of NOT has increased in South Asia and whether these levels of practice would be sustained after the pandemic subsides. METHODS: A survey was conducted among pediatric surgeons regarding their position, institute, and country; the number of appendicitis cases they managed; and their mode of treatment between identical time periods in 2019 and 2020 (April 1 to August 31). The survey also directly posed the question as to whether they would continue with the COVID-19-imposed level of NOT after the effect of the pandemic diminishes. RESULTS: A total of 134 responses were collected out of 200 (67.0%). A significant increase in the practice of NOT was observed for the entire cohort, although no effect was observed when grouped by country or institute. When grouped by position, senior physicians increased the practice of NOT the most, while junior physicians reported the least change. The data suggest that only professors would be inclined to maintain the COVID-19-level of NOT practice after the pandemic. CONCLUSIONS: Increased practice of NOT during the COVID-19 pandemic was observed in South Asia, particularly by senior surgeons. Only professors appeared inclined to consider maintaining this increased level of practice in the post-COVID-19 world.

Field demonstration of irradiated sodium alginate as tea production booster.

Sodium alginate oligomers were tested for tea plant growth promoter and anti-fungal agent in this experiment. Sodium alginate solutions were irradiated by Co-60 gamma radiation with different radiation doses to produce the oligomers. Irradiated solutions were then diluted into 150, 300 and 500 ppm prior to foliar application. Solutions were applied through foliar spraying at 7 days interval and the best response of tea plants in terms of various attributes were recorded. Tea buds were collected in 10 days of interval and the growth attributes like- total number of buds, fresh weight of buds, average leaf area and weight per bud, weight of made tea etc. were calculated. The experiment was continued up to 12 weeks and the attributes were averaged to get results per plucking. 12 kGy radiation doses along with 300ppm solution showed the best results and about 36% increase in productivity was found based on the fresh weight of buds. Total fungal count in tea leaves was also found to be reduced greatly. Based on the present study, irradiated sodium alginate could be used as safe and environmentally friendly agent to increase tea production.

Frequency of insulin resistance in nondiabetic adult Bangladeshi individuals of different obesity phenotypes.

Insulin resistance (IR) is the corner stone of metabolic obesity. This cross-sectional analytical study was aimed to find out the frequency of IR in non-diabetic adult individuals of different obesity phenotypes that would help to implement preventive measures to avoid the cardiometabolic catastrophes. METHODS: Total 955 nondiabetic adult individuals were selected and categorized into six metabolic phenotypes by metabolic syndrome criteria in each BMI group (18.5-24.9-normal weight, 25-29.9-overweight, ≥30-obese). From them, metabolically obese normal weight, metabolically obese overweight, metabolically healthy obese and metabolically unhealthy obese were selected as Obesity phenotypes (N = 616). RESULTS: The frequency of IR was found to be very high (60.2%) in total nondiabetic adult obese individuals (N = 616). Highest frequency of IR was found in MUO phenotype (76.3%), lowest frequency of IR was found in MONW phenotype (37.1%) and frequency of IR in MOOW and MHO phenotypes found to be identical but significantly (p < 0.0001) less than MUO and significantly (p < 0.0001) more than MONW phenotype. Among the obesity phenotypes, females were more insulin resistant than males (67.5% vs 48.1% respectively, p < 0.05). Frequency of IR found significantly (p < 0.05) more in female than male in all obesity phenotypes except in MUO phenotype where males found to show significantly (p < 0.05) higher frequency than females. Frequency of IR was significantly higher in younger (20-39 yrs) age group than 40-60 yrs age group (63.2% vs 53.5% respectively, p < 0.05). CONCLUSION: IR is alarmingly high (60.2%) in nondiabetic adult obese individuals. Among different obesity phenotypes, it is highest (76.3%) in MUO and lowest (37.1%) in MONW.

Biomarkers of cardiovascular and metabolic diseases in otherwise healthy overweight subjects in Bangladesh.

Biomarkers of cardiovascular and metabolic diseases were assessed in Bangladeshi overweight subjects categorized by Asian BMI criteria. After screening 300 people, 90 apparently healthy subjects were enrolled and grouped into normal weight, overweight and obese. Compared to normal weight, the overweight and obese groups showed significant elevation of serum triglyceride, insulin, homocysteine, insulin resistance/sensitivity, and atherogenic index of plasma. Cardiometabolic biomarkers did not differ between overweight and obese groups. Some biomarkers showed correlation with BMI in overweight but not in obese group. It was concluded that cardiometabolic biomarkers are elevated in Bangladeshi overweight subjects categorized by Asian BMI criteria.

Effect of jute yarn on the mechanical behavior of concrete composites.

The objective of the study is to investigate the effect of introducing jute yarn on the mechanical properties of concrete. Jute fibre is produced abundantly in Bangladesh and hence, very cheap. The investigation on the enhancement of mechanical properties of concrete with jute yarn as reinforcement, if enhanced, will not only explore a way to improve the properties of concrete, it will also explore the use of jute and restrict the utilization of polymer which is environmentally detrimental. To accomplish the objective, an experimental investigation of the compressive, flexural and tensile strengths of Jute Yarn Reinforced Concrete composites (JYRCC) has been conducted. Cylinders, prisms and cubes of standard dimensions have been made to introducing jute yarn varying the mix ratio of the ingredients in concrete, water cement ratio, length and volume of yarn to know the effect of parameters as mentioned. Compressive, flexural and tensile strength tests had been conducted on the prepared samples by appropriate testing apparatus following Standards of tests. Mechanical properties of JYRCC were observed to be enhanced for a particular range of lengths of cut (10, 15, 20 and 25 mm) and volume content of jute yarn (0.1, 0.25, 0.5 and 0.75 %). The maximum increment of compressive, flexural and tensile strengths observed in the investigation are 33, 23 and 38 %, respectively with respect to concrete without jute yarn.

Consumption of unsafe food in the adjacent area of Hazaribag tannery campus and Buriganga River embankments of Bangladesh: heavy metal contamination.

The concentrations of Cr, Cd, Pb, Hg, and As in water, poultry meat, fish, vegetables, and rice plants obtained from the area adjacent to the Hazaribag tannery campus, Dhaka, Bangladesh, were estimated and compared with permissible levels established by the WHO and FAO and levels reported previously by other authors. The metal contents were in the following order according to the concentration in contaminated irrigation water: Cr > Pb > As > Hg > Cd. Mean concentrations of Cr, Pb, Hg, and As in irrigated water were above the permissible levels, whereas the results were below the permissible levels for Cd. The metal concentrations in poultry meat, fish, rice, and vegetables were in the following orders: Pb > Cr > Cd > Hg > As, Pb > Cr > Cd > As > Hg, Pb > As, and Cr > Pb > Cd > As > Hg, respectively. The mean concentrations of metals in poultry meat, fish, rice, and vegetables were much higher than the permissible levels. The trends of weekly intake of heavy metals (WIMs) from poultry meat, fish, rice, and vegetables were as follows: Pb > Cr > Cd > Hg > As, Pb > Cr > Cd > As > Hg, Pb > As, and Cr > Pb > Cd > As > Hg, respectively. WIMs for all the metals were lower than the provisional maximum weekly intake recommended by WHO/FAO and USNAS.

Leech infestation in children through body orifices: experience in a hospital in Bangladesh.

INTRODUCTION: Bangladesh harbors many leeches in its vast wetlands. Leeches have a tendency to enter through body orifices with potentially life-threatening consequences. Literature search revealed inadequate description of clinical manifestations and treatment of leech infestations in children. We describe our experience with leech infestations in children. METHODS: Between January 1, 2004 and December 31, 2010, 17 cases of leech infestation through body orifices in children were managed. This is a retrospective study on age, sex, route of leech entry, investigation and treatment, and outcome. RESULTS: Age ranged from 4.5 to 11 years (mean 6.4 ± 1.8) and females accounted for more than 70 %. The orifices of leech entry include urethra, vagina, and rectum. Leeches could be found in eight cases. Two boys with leeches in the urinary bladder needed suprapubic removal. Leeches were retrieved from the vagina under general anesthesia in three cases, and on three occasions leeches came out from the vagina after normal saline instillation. In nine cases with different routes of entry where leech was not found, instillation of normal saline was sufficient to stop bleeding. Fifteen cases presented with bleeding and transfusion was required in five cases with Hb% <7 gm/dl. CONCLUSIONS: Leech infestation through lower body orifices is common in children of rural Bangladesh. Prompt diagnosis is of paramount importance, and application of normal saline is effective in most cases. Sometimes surgical intervention is required.

Laparoscopic appendectomy in pregnant women: experience in Chittagong, Bangladesh.

BACKGROUND: Surgery may be needed during pregnancy for nonobstetric reasons, most commonly appendicitis, and laparoscopy is considered as safe as the open method, for both mother and fetus. The aim of the present study was to emphasize the feasibility of laparoscopic appendectomy during pregnancy in a developing country, and also to report the first study of its kind from Bangladesh. MATERIALS AND METHODS: From 7 October 2005 to 6 October 2010, 31 pregnant women were diagnosed with acute appendicitis. Diagnoses were based on clinical suspicion supported by ultrasonogram. Under general endotracheal anesthesia, laparoscopy was done in all 31 cases. One gynecologist was always present to monitor the conditions of the fetus preoperatively and postoperatively. Feeding was allowed 6 h after surgery, and the majority of the patients were discharged on the second postoperative day. Age, gestational period, operative time, hospital stay, maternal and fetal outcome, and complications were evaluated. RESULTS: Age ranged from 19 to 35 years and gestational period ranged from 6 to 31 weeks. Right lower quadrant pain was the presenting complaint in majority of cases. Average operative time was 34 ± 10.19 min, and there were no conversions to open surgery. There were no intraoperative or immediate postoperative hazards. Postoperative recovery was uneventful in all cases. Histopathology of 30 cases confirmed appendicitis. One patient, whose fetus was at 12 weeks gestation at the time of the appendectomy had a spontaneous abortion 1 month later. There were no adverse outcomes during the follow-up period. CONCLUSIONS: Laparoscopy is a safe and effective technique for the treatment of appendicitis during pregnancy and can be performed in a developing country.

Cystoscopy-assisted percutaneous removal of impacted urethral stone: a case report.

BACKGROUND AND PURPOSE: Although cystolitholapaxy is possible in children, the instruments are not available everywhere. For tiny impacted urethral stone, an alternate technique is described. CASE REPORT: A 3-year-old boy came with retention of urine and dysuria. On examination: bladder was full, suprapubic region was tender, and a stone could be felt at posterior urethra. We planned push back and suprapubic cystolithotomy. After general anesthesia, the stone was pushed back by instillation of lignocaine jelly into the urethra. Then, a cystoscope was introduced, and a stone was found inside the urinary bladder. Under cystoscopic view, a 5 mm laparoscopic port was inserted into the bladder just above the pubic symphysis in the midline. A 5 mm tissue holding forceps was introduced through this port, and the tiny stone was grasped and brought out along with the port. The port site was closed by a single stitch. A penile catheter was kept for 5 days. RESULTS: The boy did well in the postoperative period, and voiding was normal after removal of the catheter. CONCLUSIONS: Impacted posterior urethral stone can be retrieved by simple percutaneous technique.

Intestinal obstruction in children due to segmental enteritis: experience in Chittagong, Bangladesh.

INTRODUCTION: Acute segmental enteritis is also known as acute segmental necrotizing enteritis, segmental obstructing acute jejunitis, and enteritis necroticans. Children presenting with high fever, P/R bleeding, prostration etc. along with features of intestinal obstruction are not uncommon. Many of these cases had to be operated upon revealing no definite obstructing lesion, except a segment of small gut with different magnitude of inflammation. The principal author encountered many of these cases in the same hospital and also in the previous working places. This study was thus undertaken and this is the first report from Bangladesh on segmental enteritis. MATERIALS AND METHODS: From 1 January 2004 to 31 December 2009, 24 consecutive cases suffering from enteritis were managed. This prospective descriptive study evaluates the sex, age, presentations, physical findings, operative findings, hospital stay, outcome and complications. The inclusion criteria were an acute abdominal presentation, plain radiographic features of intestinal obstruction and intraoperative findings of segmental small intestinal involvement with ischemic lesions without definite obstruction. RESULTS: Fever and leucocytosis were present in all, abdominal tenderness in 17 and per-rectal bleeding in 15 cases. Jejunum alone was involved in 17, both jejunum and ileum in 5 and ileum alone in 2 cases. Scattered dark patches over the intestinal wall was found in 16, circumferential dusky lesions in 4, perforation in one and frank gangrene in 3 cases. Bacteriology of the peritoneal fluid in 17 cases revealed mixed flora. Histopathology of the mesenteric lymph nodes in 11 cases showed non-specific hyperplasic changes. Histopathology of four resected gut walls showed inflammatory infiltrates. In 17 cases recovery was uneventful. Seven cases suffered different complications including two (8.33%) deaths. One with ileal stricture and another with fecal fistula required reoperation. Mean hospital stay was 10.63 ± 3.00 days. The surviving patients were followed up for at least 6 months and faring well. CONCLUSIONS: Segmental enteritis is occasionally encountered as a cause of acute abdomen in children of Bangladesh. Operative treatment is required and mechanical obstructing lesion was found in none of the cases. Majority of cases improve on intraoperative measures other than gut resections or creation of stoma.

Laparoscopic cholecystectomy without handling the cystic artery: a new approach to minimize complications.

INTRODUCTION: Although laparoscopy has now become the procedure of choice for removal of gallbladder, complications especially injury to cystic artery and ducts are thought to be higher. We describe a technique that avoids handling and injury of cystic artery and resultant duct injuries. The video length is 2 minutes 13 seconds. MATERIALS AND METHODS: From October 7, 2005, to December 31, 2010, 17 patients were successfully operated by the cystic artery sparing technique. In this technique, dissection begins distal to cystic lymph node on the gallbladder wall using hook cautery, thereby clears the Calot's triangle without even touching the cystic artery. This maneuver eliminates the risk of injuring thus causing bleeding and any subsequent complications. Age, sex, and operative outcomes including follow-ups were evaluated. RESULTS: Age of the patients ranged from 3.4 years to 16 years with an average of 10.12 years. Males were predominant (10:7). Average operative time was 40 minutes. There were no intraoperative or immediate postoperative complications. Patients were allowed feeding 4-6 hours after operation and discharged the following morning. Patients were asked to come for follow-up at 1 week, 1 month, 3 months, 6 months, and 1 year and thereafter yearly. Follow-up period ranged from 6 months to 5 years. There were no operation-related complications other than minor abdominal pain in 3 patients and port-site pain in 2 patients which resolved on conservative treatments. CONCLUSION: During laparoscopic cholecystectomy, if handling of the main cystic artery is avoided then complications of hemorrhage and bile duct injuries can be minimized.

Serum homocysteine in pre-eclampsia and eclampsia.

Pre-eclampsia and eclampsia are common obstetrical problem causing adverse effects on pregnancy outcome. Large bodies of evidences suggest that hyperhomocysteinemia is a causal factor of pre-eclampsia/eclampsia. This study designed to explore the association between hyperhomocysteinemia and pre-eclampsia/eclampsia, the knowledge of which expected to be used for prevention of pre-eclampsia and eclampsia. In a case-control study serum homocysteine was measured in 136 controls (healthy pregnant), 84 pre-eclamptic and 120 eclamptic pregnant women. Serum homocysteine in patients with pre-eclampsia (9.54 +/- 3.21 micromol/L) and eclampsia (10.57 +/- 3.39 micromol/L) found to be significantly increased compared to controls (6.86 +/- 2.47 micromol/L) (p < 0.001). Between pre-eclampsia and eclampsia, homocysteine found to be raised more in eclampsia compared to pre-eclampsia (p < 0.03). In conclusion, hyperhomocysteinemia is associated with pre-eclampsia as well as eclampsia, but in eclampsia the severity of homocysteine elevation is more compared to that in pre-eclampsia.

HIV transmission in Bangladesh: an analysis of IDU programme coverage.

BACKGROUND: Sentinel Surveillance found HIV had increased to 7% among street-based injection drug users (IDUs) in Central Bangladesh in 2006-2007, indicating the urgent need to increase prevention. In 2004, size estimation of groups vulnerable to HIV was done by an expert committee under the National AIDS/STD Programme, making programme coverage estimates possible for the first time. METHODS: Appropriate multipliers were applied to size information to estimate a total of 20,000-40,000 IDUs throughout the country. Data from 2003 onwards from the major needle-exchange programmes (NEPs) and detoxification services were analysed to estimate programme coverage as the proportion of the total IDU population reached by interventions, and to assess the proportion of safe injections. RESULTS: An estimated 31-61% of the upper and lower national size estimates of IDUs, respectively, were in contact with any HIV prevention effort by 2006, with an increasing trend evident over the years. Of these, 24-49% were enrolled at NEPs, and 8-16% had been through detoxification. Although there was a marked improvement over the years, it appears NEPs only provided 160 days of safe injections for every IDU reached in 2006 in the North and Southeast, and 50 days in the South and Southwest, assuming IDU inject twice daily with new needles. If all IDUs reached by programmes in the North and Southeast injected every day, 44% of injections were covered by new needles, and 14% in the South and Southwest. CONCLUSION: Within the context of an HIV epidemic among some IDU, and high levels of needle-sharing and risky sexual behaviour, the implications of the low level of programme coverage are alarming, and it is clear Bangladesh needs to take action to improve it in order to control the spread of HIV.

Seasonal changes in canopy photosynthesis and respiration, and partitioning of photosynthate, in rice (Oryza sativa L.) grown under free-air CO2 enrichment.

An increase in atmospheric CO(2) concentration ( [CO(2)]) is generally expected to enhance photosynthesis and biomass. Rice plants (Oryza sativa L.) were grown in ambient CO(2) (AMB) or free-air CO(2)-enrichment (FACE), in which the target [CO(2)] was 200 micromol mol(-1) above AMB. (13)CO(2) was fed to the plants at different stages so we could examine the partitioning of photosynthates. Furthermore, canopy photosynthesis and respiration were measured at those stages. The ratio of (13)C content in the whole plant to the amount of fixed (13)C under FACE was similar to that under AMB at the vegetative stage. However, the ratio under FACE was greater than the ratio under AMB at the grain-filling stage. At the vegetative stage, plants grown under FACE had a larger biomass than those grown under AMB owing to enhancement of canopy photosynthesis by the increased [CO(2)]. On the other hand, at the grain-filling stage, CO(2) enrichment promoted the partitioning of photosynthate to ears, and plants grown under FACE had a greater weight of ears. However, enhancement of ear weight by CO(2) enrichment was not as great as that of biomass at the vegetative stage. Plants grown under FACE did not necessarily show higher canopy photosynthetic rates at the grain-filling stage. Therefore, we concluded that the ear weight did not increase as much as biomass at the vegetative stage owing to a loss of the advantage in CO(2) gain during the grain-filling period.