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1.
Elife ; 62017 05 05.
Artigo em Inglês | MEDLINE | ID: mdl-28475038

RESUMO

Investigations into stem cell-fueled renewal of an organ benefit from an inventory of cell type-specific markers and a deep understanding of the cellular diversity within stem cell niches. Using the adult mouse incisor as a model for a continuously renewing organ, we performed an unbiased analysis of gene co-expression relationships to identify modules of co-expressed genes that represent differentiated cells, transit-amplifying cells, and residents of stem cell niches. Through in vivo lineage tracing, we demonstrated the power of this approach by showing that co-expression module members Lrig1 and Igfbp5 define populations of incisor epithelial and mesenchymal stem cells. We further discovered that two adjacent mesenchymal tissues, the periodontium and dental pulp, are maintained by distinct pools of stem cells. These findings reveal novel mechanisms of incisor renewal and illustrate how gene co-expression analysis of intact biological systems can provide insights into the transcriptional basis of cellular identity.


Assuntos
Perfilação da Expressão Gênica , Incisivo/citologia , Células-Tronco/fisiologia , Animais , Biomarcadores/análise , Proteínas de Transporte/análise , Linhagem da Célula , Glicoproteínas de Membrana/análise , Camundongos , Proteínas do Tecido Nervoso/análise
2.
Artigo em Inglês | MEDLINE | ID: mdl-18442730

RESUMO

OBJECTIVE: This study was designed to compare mesenchymal stem cell (MSC)-based alveolar bone regeneration in biphasic bone substitutes and natural bone mineral in a canine full-thickness alveolar defect model. MATERIALS AND METHODS: MSCs were isolated from bone marrow aspirates and culture expanded through 3 successive subcultures. The bone differentiation potential of third passage cells was evaluated and confirmed in vitro before cells were used in the transplantation experiment. Undifferentiated cells were then incubated with 3 x 3 x 3 mm(3) hydroxyapatite/beta-tricalcium phosphate (HA/TCP) matrices (Kasios, Lanauguet, France) and 1- to 2-mm Bio-Oss spongiosa (Geistlich Biomaterials, Osteohealth, Switzerland), which is a natural bovine bone mineral (NBM). Kasios/cell, Kasios alone, Bio-Oss/cell, and Bio-Oss alone were implanted in masseter muscle and 4 cylindrical (10-mm diameter) through-and-through bilateral mandibular body defects in 4 mongrel dogs. Histomorphometric analysis was performed 6 weeks after insertion of the scaffold loaded with MSCs. RESULTS: H&E staining of the decalcified scaffold and scanning electron microscopy demonstrated large MSC coverage of the HA/TCP and Bio-Oss. Cell-loaded Kasios matrices showed the greatest amount of the bone regeneration among the groups in both the muscle (29.11%) and the bone specimens (65.78%). Cell-free biphasic scaffold revealed 44.9% bone fill in bone defects and 23.55% in muscle specimen, and Bio-Oss alone matrices had the least amount of new bone formation: 36.84% and 24.16% in bone and muscle specimens respectively. Kasios loaded with MSCs demonstrated more bone regeneration than Bio-Oss/cell but there was no significant statistical difference (P > .05). CONCLUSIONS: New biphasic synthetic bone substitutes may offer better conditions for bone regeneration than traditional bone substitute in combination with MSCs. They remained in the defect and contributed to bone regeneration.


Assuntos
Transplante de Medula Óssea/métodos , Regeneração Óssea , Substitutos Ósseos , Transplante de Células-Tronco Mesenquimais/métodos , Alicerces Teciduais , Animais , Transplante Ósseo , Fosfatos de Cálcio , Técnicas de Cultura de Células , Diferenciação Celular , Células Cultivadas , Técnica de Descalcificação , Cães , Durapatita , Mandíbula/cirurgia , Minerais , Osteoblastos/fisiologia
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