Transcriptome Analysis of Plasmodium falciparum Isolates From Benin Reveals Specific Gene Expression Associated With Cerebral Malaria.

Cerebral malaria (CM) is the severest form of Plasmodium falciparum infection. Children under 5 years old are those most vulnerable to CM, and they consequently have the highest risk of malaria-related death. Parasite-associated factors leading to CM are not yet fully elucidated. We therefore sought to characterize the gene expression profile associated with CM, using RNA sequencing data from 15 CM and 15 uncomplicated malaria isolates from Benin. Cerebral malaria parasites displayed reduced circulation times, possibly related to higher cytoadherence capacity. Consistent with the latter, we detected increased var genes abundance in CM isolates. Differential expression analyses showed that distinct transcriptome profiles are signatures of malaria severity. Genes involved in adhesion, excluding variant surface antigens, were dysregulated, supporting the idea of increased cytoadhesion capacity of CM parasites. Finally, we found dysregulated expression of genes in the entry into host pathway that may reflect greater erythrocyte invasion capacity of CM parasites.

[Depression, anxiety and coping strategies in adult patients with cystic fibrosis]. / Depression, anxiété et stratégies de "coping" chez les adultes atteints de mucoviscidose.

The considerable progress that has been made in the treatment of cystic fibrosis has significantly increased life expectancy. The multi-disciplinary approach is now considered to be a priority by centers providing care for adults with cystic fibrosis. The aim of this study was to investigate three psychological morbidities that are commonly present with this disease: depression, anxiety and poor coping mechanisms for stress. Sixteen subjects (aged 19 to 37 years) participated in this study and were divided in three groups according to the severity of their pulmonary disease. Assessments of depression and anxiety were made from two perspectives: 1) the subject's own perception of his/her depressive and anxious state; 2) a psychologist's assessment of the patients depressive and anxious state. Then, reactions to stress were explored by analyzing "coping" strategies employed. Results indicate that patients underestimated the level of their depressive and anxious state when compared to the psychologists assessment. The majority of them exhibited "coping" strategies based on emotional responses. Levels of anxiety and depression did not show any relationship with the severity of pulmonary disease suggesting that the major determinant of emotional well-being lies with the individual's own perception of their state and of the support available to them.