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1.
Science ; 374(6572): 1221-1227, 2021 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-34855475

RESUMO

Increased blood levels of low-density lipoprotein cholesterol (LDL-C) and fibrinogen are independent risk factors for cardiovascular disease. We identified associations between an Amish-enriched missense variant (p.Asn352Ser) in a functional domain of beta-1,4-galactosyltransferase 1 (B4GALT1) and 13.9 milligrams per deciliter lower LDL-C (P = 4.1 × 10­19) and 29 milligrams per deciliter lower plasma fibrinogen (P = 1.3 × 10­5). B4GALT1 gene­based analysis in 544,955 subjects showed an association with decreased coronary artery disease (odds ratio = 0.64, P = 0.006). The mutant protein had 50% lower galactosyltransferase activity compared with the wild-type protein. N-linked glycan profiling of human serum found serine 352 allele to be associated with decreased galactosylation and sialylation of apolipoprotein B100, fibrinogen, immunoglobulin G, and transferrin. B4galt1 353Ser knock-in mice showed decreases in LDL-C and fibrinogen. Our findings suggest that targeted modulation of protein galactosylation may represent a therapeutic approach to decreasing cardiovascular disease.


Assuntos
LDL-Colesterol/sangue , Fibrinogênio/análise , Galactosiltransferases/genética , Mutação de Sentido Incorreto , Animais , Doença da Artéria Coronariana/genética , Doença da Artéria Coronariana/prevenção & controle , Feminino , Galactose/metabolismo , Galactosiltransferases/metabolismo , Técnicas de Introdução de Genes , Técnicas de Silenciamento de Genes , Glicoproteínas/sangue , Glicosilação , Humanos , Fígado/enzimologia , Masculino , Camundongos , Ácido N-Acetilneuramínico/metabolismo , Polissacarídeos/sangue , Sequenciamento Completo do Genoma
2.
PLoS One ; 14(4): e0215911, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31039173

RESUMO

For most complex traits, the majority of SNPs identified through genome-wide association studies (GWAS) reside within noncoding regions that have no known function. However, these regions are enriched for the regulatory enhancers specific to the cells relevant to the specific trait. Indeed, many of the GWAS loci that have been functionally characterized lie within enhancers that regulate expression levels of key genes. In order to identify polymorphisms with potential allele-specific regulatory effects, we developed a bioinformatics pipeline that harnesses epigenetic signatures as well as transcription factor (TF) binding motifs to identify putative enhancers containing a SNP with potential allele-specific TF binding in linkage disequilibrium (LD) with a GWAS-identified SNP. We applied the approach to GWAS findings for blood lipids, revealing 7 putative enhancers harboring associated SNPs, 3 of which lie within the introns of LCAT and ABCA1, genes that play crucial roles in cholesterol biogenesis and lipoprotein metabolism. All 3 enhancers demonstrated allele-specific in vitro regulatory activity in liver-derived cell lines. We demonstrated that these putative enhancers are in close physical proximity to the promoters of their respective genes, in situ, likely through chromatin looping. In addition, the associated alleles altered the likelihood of transcription activator STAT3 binding. Our results demonstrate that through our approach, the LD blocks that contain GWAS signals, often hundreds of kilobases in size with multiple SNPs serving as statistical proxies to the true functional site, can provide an experimentally testable hypothesis for the underlying regulatory mechanism linking genetic variants to complex traits.


Assuntos
Transportador 1 de Cassete de Ligação de ATP/genética , Alelos , HDL-Colesterol/metabolismo , Elementos Facilitadores Genéticos , Fosfatidilcolina-Esterol O-Aciltransferase/genética , Sequência de Bases , Linhagem Celular , Cromatina/metabolismo , Estudo de Associação Genômica Ampla , Humanos , Interleucina-6/genética , Interleucina-6/metabolismo , Fígado/metabolismo , Polimorfismo de Nucleotídeo Único/genética , Regiões Promotoras Genéticas , Ligação Proteica , Elementos de Resposta/genética , Fator de Transcrição STAT3/metabolismo
3.
Circulation ; 138(13): 1343-1355, 2018 09 25.
Artigo em Inglês | MEDLINE | ID: mdl-29593015

RESUMO

BACKGROUND: Elevated levels of low-density lipoprotein cholesterol (LDL-C) are a major risk factor for cardiovascular disease via its contribution to the development and progression of atherosclerotic lesions. Although the genetic basis of LDL-C has been studied extensively, currently known genetic variants account for only ≈20% of the variation in LDL-C levels. METHODS: Through an array-based association analysis in 1102 Amish subjects, we identified a variant strongly associated with LDL-C levels. Using a combination of genetic analyses, zebrafish models, and in vitro experiments, we sought to identify the causal gene driving this association. RESULTS: We identified a founder haplotype associated with a 15 mg/dL increase in LDL-C on chromosome 5. After recombination mapping, the associated region contained 8 candidate genes. Using a zebrafish model to evaluate the relevance of these genes to cholesterol metabolism, we found that expression of the transcribed pseudogene, APOOP1, increased LDL-C and vascular plaque formation. CONCLUSIONS: Based on these data, we propose that APOOP1 regulates levels of LDL-C in humans, thus identifying a novel mechanism of lipid homeostasis.


Assuntos
Amish/genética , Aterosclerose/genética , LDL-Colesterol/sangue , Cromossomos Humanos Par 5 , Dislipidemias/genética , Pseudogenes , Animais , Animais Geneticamente Modificados , Aterosclerose/sangue , Aterosclerose/diagnóstico , Aterosclerose/etnologia , Dislipidemias/sangue , Dislipidemias/diagnóstico , Dislipidemias/etnologia , Efeito Fundador , Estudos de Associação Genética , Predisposição Genética para Doença , Haplótipos , Humanos , Fenótipo , Recombinação Genética , Fatores de Risco , Peixe-Zebra/genética
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