A Multi-Stage Planning Method for Distribution Networks Based on ARIMA with Error Gradient Sampling for Source-Load Prediction.

As the scale of distributed renewable energy represented by wind power and photovoltaic continues to expand and load demand gradually changes, the future evolution of the smart distribution network will be directly driven by both distributed generation and user demand. The smart distribution network contains a wide range of flexible resources, and its flexibility and uncertainty will bring great challenges to grid data acquisition and control feedback. To adapt to the precise control and feedback of smart distribution network access equipment under the high proportion of new energy access and to ensure the safe operation of the system, it is urgent to accelerate the study of the evolution of the future distribution grid based on the existing distribution grid. Hence, a multi-stage planning method for distribution networks based on source-load prediction is proposed in this paper. Firstly, a distribution network source-load prediction method based on the autoregressive integrated moving average model (ARIMA) and error gradient sampling is proposed, using ARIMA to predict the scale of source-load development and error gradient sampling based on the generation of source-load scenarios with error intervals. K-means is further used for scenario reduction, to explore multiple operating scenarios of China's distribution network source-load, and the unit's output forecast interval and load demand from 2021 to 2030 for typical regions are derived using rolling forecasts by combining the unit's output, end-demand and clean energy share over the years. Secondly, the planning model of distribution grid evolution in different stages is constructed to analyze the future evolution form of the distribution grid considering the distribution network's load cross-section, respectively, and to provide a development path reference for the future construction of distribution grid form in China.

Biguanide MC001, a Dual Inhibitor of OXPHOS and Glycolysis, Shows Enhanced Antitumor Activity Without Increasing Lactate Production.

Metformin and other biguanides represent a new class of inhibitors of mitochondrial complex I that show promising antitumor effects. However, stronger inhibition of mitochondrial complex I is generally associated with upregulation of glycolysis and higher risk of lactic acidosis. Herein we report a novel biguanide derivative, N-cystaminylbiguanide (MC001), which was found to inhibit mitochondrial complex I with higher potency while inducing lactate production to a similar degree as metformin.Furthermore, MC001 was found to efficiently inhibit a panel of colorectal cancer (CRC) cells in vitro and to suppress tumor growth in a HCT116 xenograft nude mouse model, while not enhancing lactate production relative to metformin, exhibiting a superior safety profile to other potent biguanides such as phenformin. Mechanistically, MC001 efficiently inhibits mitochondrial complex I, activates AMPK, and represses mTOR, leading to cell-cycle arrest and apoptosis. Notably, MC001 inhibits both oxidative phosphorylation (OXPHOS) and glycolysis. We therefore propose that MC001 warrants further investigation in cancer treatment.

Targeting Extracellular Cyclophilin A via an Albumin-Binding Cyclosporine A Analogue.

An albumin-binding CsA analogue 4MCsA was achieved by attachment of a thiol-reactive maleimide group at the side-chain of P4 position of CsA derivative. 4MCsA was semi-synthesized from CsA, and the cell-impermeability of albumin-4MCsA was detected by mass spectrometry and a competitive flow cytometry. 4MCsA exhibits inhibition of chemotaxis activity and inflammation by targeting extracellular CypA without immunosuppressive effect and cellular toxicity. These combined results suggested that 4MCsA can be restricted extracellularly through covalently binding to Cys34 of albumin with its maleimide group, and regulate the functions of cyclophilin A extracellularly.

Genome reanalysis to decipher resistome, virulome, and attenuated characters of attenuated Streptococcus agalactiae strain HZAUSC001.

Streptococcus agalactiae is a serious pathogen causing severe anthropozoonosis in a broad range of hosts, from aquatic animals to mammals, including humans. S. agalactiae HZAUSC001 was isolated from a moribund tilapia fish exhibiting classic clinical symptoms of streptococcosis in Zhanjiang, Guangdong, China. And it was identified as the etiological factor resulting in fish disease, but was notable because it exhibited attenuated virulence. Here, the genome of S. agalactiae HZAUSC001 was re-analyzed; we assessed the resistome and virulome and deciphered the attenuated characters of HZAUSC001. The S. agalactiae HZAUSC001 genome was assembled into one chromosome with a GC-content of 35.37% and 1972 predicted open reading frames (ORFs). Phylogenetic analysis indicated that it is evolutionarily similar to piscine GBS strains GD201008-001 and ZQ0910. After re-analyzing the published genomic sequence of HZAUSC001, we identified 38 virulence factor genes and one antibiotic-resistance gene. Note that three previously noted virulence genes, bca (C protein alpha-antigen), cpbA (choline-binding protein A) and esp (enterococcal surface protein), were absent in the virulence-attenuated strain S. agalactiae HZAUSC001 but present in the highly virulent strain S. agalactiae GD201008-001. We speculate that the absence of these three virulence genes may be associated with the attenuated traits of the HZAUSC001 strain. Collectively, our study supports that HZAUSC001 may be an excellent candidate for development of an attenuated vaccine, and our results contribute to further understanding of GBS epidemiology and surveillance targets.

Comparative transcriptome profiling reveals a mechanism of Streptococcus agalactiae resistance to florfenicol.

Florfenicol is widely used to control diseases in aquatic animals, and is used extensively to treat streptococcosis-caused by Streptococcus agalactiae-in the commercially important fish tilapia. There are known issues with the development of florfenicol resistance in Streptococcus agalactiae, but the underlying resistance mechanisms are not clear, a situation currently preventing optimal deployment of antibiotics. Here, we examined the induction of resistance by successively increasing the concentrations of florfenicol, and then used RNA-sequencing (RNA-Seq) to characterize changes in the transcriptomes of a florfenicol-resistant strain (H51-R) and a florfenicol-sensitive strain (H51-S). We obtained a total of 18,418,068 sequence reads in H51-R and 16,070,122 sequence reads in H51-S, from which a total of 1940 unigenes were assembled. In total, 376 unigenes were found to be differently expressed genes (DEGs). After florfenicol treatment, 181 genes were upregulated and 195 genes were downregulated. GO functional analysis of the DEGs indicated that the most strongly enriched GO terms included metabolic process (152 genes), catalytic activity (146), and binding (133), with terms including membrane, membrane part, and transporter activity also showing enrichment. KEGG pathway enrichment analysis highlighted that ribosomes were prominently involved in the transcriptional changes associated with florfenicol resistance. This study demonstrates that florfenicol treatment affects multiple biological functions of Streptococcus agalactiae, suggests that florfenicol resistance in Streptococcus agalactiae is closely related to the reduction of intracellular drug accumulation caused by ATP-binding cassette (ABC) transporters, and highlights the potential involvement of altered ribosomal function in florfenicol resistance.

Structure and function analysis of various brain subregions and pituitary in grass carp (Ctenopharyngodon idellus).

It has been generally acknowledged that environment could alter the morphology and functional differentiation of vertebrate brain. However, as the largest group of all vertebrates, studies about the structures and functions of various brain subregions in teleost are still scarce. In this study, using grass carp as a model, histology method and RNA-sequencing were recruited to examine the microstructure and transcript levels among different brain subregions and pituitary. Histological results showed that the grass carp brain was composed of six parts, including olfactory bulb, telencephalon, hypothalamus, optic tectum, cerebellum, and medulla oblongata. In addition, compared to elasmobranchs and non-teleost bony ray-finned fishes, grass carp lost the hypothalamo-hypophyseal portal system, instead the hypophysiotropic neurons were directly terminated in the pituitary cells. At the transcriptomic level, our results suggested that the olfactory bulb might be related to reproduction and immune function. The telencephalon was deemed to be involved in the regulation of appetite and reproduction. The optic tectum might play important roles in the vision system and feeding. The hypothalamus could regulate feeding, and reproduction process. The medulla oblongata was related with the auditory system. The pituitary seemed to play pivotal roles in energy metabolism, organ development and reproduction. Finally, the correlation analysis suggested that the hypothalamus and the telencephalon were highly related, and close anatomical connection and overlapping functions suggested that the telencephalon and hypothalamus might be the regulation center of feeding and reproduction among teleost brain. This study provided a global view of the microstructures and specific functions of various brain subregions and pituitary in teleost. These results will be very helpful for further study in the neuroendocrinology regulation of growth and reproduction in teleost brain-pituitary axis.

The pathogenic and antimicrobial characteristics of an emerging Streptococcus agalactiae serotype IX in Tilapia.

Streptococcus agalactiae (S. agalactiae, GBS) infection has caused significant economic loss in the tilapia aquaculture, which is one of the most important commercial fish worldwide. Among the 10 serotypes of GBS, serotypes Ia, Ib, II and III were epidemic in tilapia while serotype IX has never been found in tilapia before. In this study, 80 strains isolated from moribund tilapia in China were identified as GBS. All the isolates have been classified as serotype III or serotype IX of GBS. Unexpectedly, the serotype IX has never been reported in fish, but it was epidemic in mammals. Antimicrobial resistance results showed that serotype IX but not III was resistant to streptomycin and erythromycin. Artificial infection results showed that both serotypes could cause serious pathological injuries in the infected tissues of tilapia. Furthermore, serotype IX instead of serotype III, mainly infected the brain of tilapia. The results will shed a new light on the epidemic and pathogenicity of GBS, and will pave a new way for the prevention of Streptococcosis in tilapia.

A standing wave ultrasonic stepping motor using open-loop control system.

We propose a standing wave ultrasonic stepping motor with blades and grooves for positioning. The step-by-step movements are achieved by applying the square wave driving voltage and the high-voltage pulse in turn. The prototype with a diameter of 30mm, a height of 10mm is experimentally characterized. The motor is superior to those previously reported due to the positioning mechanism for eliminating displacement cumulative error when using an open-loop control system. Also, the operating mode and the metal-to-metal friction drive ensure a stall torque of 0.055Nm under a relatively low operating voltage of 100Vpp at a resonance frequency of 74kHz.

GapA, a potential vaccine candidate antigen against Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus).

Streptococcosis due to the bacterium Streptococcus agalactiae (S. agalactiae) has resulted in enormous economic losses in aquaculture worldwide, especially in the tilapia culture industry. Previously, there were limited vaccines that could be employed against streptococcosis in tilapia. This study aimed to develop a vaccine candidate using the glyceraldehyde-phosphate dehydrogenase protein (GapA) of S. agalactiae encoded by the gapA gene. Tilapia were intraperitoneally injected with PBS, PBS + Freund's adjuvant, PBS + Montanide's adjuvant, GapA + Freund's adjuvant, GapA + Montanide's adjuvant, killed S. agalactiae whole cells (WC)+Freund's adjuvant, or killed S. agalactiae whole cells (WC)+ Montanide's adjuvant. They were then challenged with S. agalactiae, and the relative percentage survival (RPS) was monitored 14 days after the challenge. The highest RPSs were observed in the WC groups, with 76.7% in WC + Freund's adjuvant and 74.4% in WC + Montanide's adjuvant groups; these were followed by the GapA groups, with 63.3% in GapA + Freund's adjuvant and 45.6% in GapA + Montanide's adjuvant groups. The RPS of the PBS group was 0%, and those of PBS + Freund's adjuvant and PBS + Montanide's adjuvant groups were 6.7% and 3.3%, respectively. Additionally, the IgM antibody responses elicited in GapA groups and WC groups were significantly higher than those in PBS groups. Furthermore, the expressions of cytokine (IL-1ß and TNF-α) mRNAs in the GapA groups and WC groups were significantly higher than those in the PBS groups. Taken together, these results reveal that the GapA protein is a promising vaccine candidate that could be used to prevent streptococcosis in tilapia.

Draft Genome Sequence of an Attenuated Streptococcus agalactiae Strain Isolated from the Gut of a Nile Tilapia (Oreochromis niloticus).

Streptococcus agalactiae is a pathogen that causes severe anthropozoonosis within a broad range of hosts from aquatic animals to mammals, including human beings. Here, we describe the draft genome of S. agalactiae HZAUSC001, a low-virulent strain isolated from the gut of a moribund tilapia (Oreochromis niloticus) in China.

Driving frequency optimization of a piezoelectric transducer and the power supply development.

Piezoelectric transducers are commonly operated at their resonance frequency. However, from a power dissipation standpoint, this is not the ideal driving frequency. In this paper, an optimized driving frequency in between the resonance and antiresonance frequencies is proposed for the piezo-transducer. First, the optimum driving frequency is characterized using a constant vibration velocity measurement method. The actual input power reveals the lowest power dissipation frequency between the resonance and the antiresonance frequencies, where the transducer behaves inductive. The electrical parameters of the transducer are then determined by an equivalent circuit formulation, which is useful for the electrical circuit analysis of the driver design. A Class E resonant inverter is used to design a capacitive output impedance driver at the optimized frequency by utilizing a series capacitor. Compared with the traditional resonance drive, driving at the optimized frequency reduces the required power by approximately half according to the measurements performed.