Aberrant Expression of miR-100 in Plasma of Patients with Osteoporosis and its Potential Diagnostic Value.

BACKGROUND: Osteoporosis is one of the most commonly diagnosed age-related bone diseases worldwide, and it is also one of the leading causes of fracture. MicroRNAs (miRNAs) are critical molecular regulators that are involved in the bone re-modelling processes, and the circulating miRNAs were stable in the peripheral blood. Thus, to detect the level of miRNAs in plasma of osteoporotic patients may be an efficient, repeatable, and inexpensive method for the early diagnosis and evaluation of the therapeutic efficacy of osteoporosis. The aim of the present study was to investigate the potential diagnostic value of miR-100 in plasma of patients with osteoporosis. METHODS: A total of 120 osteoporotic patients were recruited and 120 healthy individuals were also included as the control group. The plasma of the participants was collected and the RNAs were extracted. The expressions of miR-100 in different clinical samples were examined using the RT-qPCR method. Furthermore, receiver operating characteristics curve (ROC) was drawn to determine the diagnostic value of miR-100 for osteoporosis. Next, the correlation between the plasma levels of miR-100 and T-scores of the patients were evaluated and, finally, the correlation between the plasma level of miR-100 and the expression levels of 25OH-D2 and 25OH-D3 were analyzed. RESULTS: miR-100 was significantly increased in plasma of patients with osteoporosis in comparison with healthy individuals; moreover, results of ROC analysis indicated that plasma level of miR-100 is a sensitive biomarker that could distinguish osteoporosis patients from healthy controls (AUC, 0.8916, 95% confidence interval (CI), 0.8468 to 0.9364). Furthermore, miR-100 was found to be negatively correlated with both vBMD (r = -0.3117, p = 0.0005) and Lumbar Spine L2-L4 T-score in patients with osteoporosis (r = -0.2929, p = 0.012). Finally, the plasma level of miR-100 was negatively correlated with the levels of 25OH-D2 (r = -0.3002, p = 0.0008) and 25OH-D3 (r = -0.3105, p = 0.0006) of the osteoporotic patients. CONCLUSIONS: miR-100 was abnormally increased in the plasma of osteoporotic patients, suggesting that circulating miR-100 could serve as potential biomarker for the diagnosis and treatment osteoporosis.

[Comparison of short-term result between high-flex and conventional posterior-stabilized prosthesis in total knee arthroplasty].

OBJECTIVE: To compare the short-term result between the high-flex (HF) and conventional posterior-stabilized (PS) prosthesis in total knee arthroplasty (TKA). METHODS: From April 2005 to October 2007, 23 cases (27 knees) underwent TKA by HF prosthesis (HF group), and 35 cases (41 knees) underwent TKA by PS prosthesis (PS group). In HF group, there were 2 males (3 knees) and 21 females (24 knees) aged (64.3 +/- 5.6) years, including 20 cases (23 knees) of osteoarthritis and 3 cases (4 knees) of rheumatoid arthritis; body mass index (BMI) was 27.3 +/- 3.9; the course of disease was (5.3 +/- 5.6) years; the Hospital for Special Surgery Scoring System (HSS) score was 58.4 +/- 7.9; the Western Ontario and McMaster universities osteoarthritis index (WOMAC) score was 49.4 +/- 6.9; the maximum knee flex degree was (107.6 +/- 8.3) degrees; and the range of knee motion was (103.5 +/- 7.7) degrees. In PS group, there were 3 males (3 knees) and 32 females (38 knees) aged (65.1 +/- 5.9) years, including 33 cases (39 knees) of osteoarthritis and 2 cases (2 knees) of rheumatoid arthritis; BMI was 27.1 +/- 4.1; the course of disease was (5.1 +/- 4.9) years; HSS score was 60.1 +/- 10.4; WOMAC score was 47.9 +/- 7.2; the maximum knee flex degree was (108.4 +/- 9.7) degrees; and the range of knee motion was (105.9 +/- 11.4) degrees. There were no significant differences in general data between two groups (P > 0.05). RESULTS: All incisions achieved healing by first intention. No complication of ankylosis, blood vessel and nerve injuries, and prosthesis loosening occurred. All patients were followed up for 24-54 months (average 32.8 months). There were no significant differences in the HSS score, WOMAC score, the maximum knee flex degrees, and the range of knee motion at 3, 12, and 24 months after operation between two groups (P > 0.05), but there were significant differences between pre- and post-operation (P < 0.05). Anterior knee pain occurred in 1 case of HF group and 4 cases of PS group after 24 months, the incidence rates were 3.70% in HF group and 9.76% in PS group, showing significant difference (P < 0.05). The X-ray films showed that no lucent zone around prosthesis and no patella baja were observed, and the force line was excellent. CONCLUSION: There is no significant difference in the range of knee motion and clinical scores between the HF prosthesis and the PS prosthesis, but the former's incidence rate of anterior knee pain is lower.