Macrogenomic analysis of the effects of aqueous-phase from hydrothermal carbonation of sewage sludge on nitrogen metabolism pathways and associated bacterial communities during composting.

The effects of aqueous phases (AP) formed from hydrothermal carbonation of sewage sludge (with or without rice husk) as moisture regulators of nitrogen metabolism pathways during composting are currently unclear. Macrogenomic analyses revealed that both APs resulted in notably changes in bacterial communities during composting; increased levels of nitrogen assimilation, nitrification, and denitrification metabolic pathways; and decreased levels of nitrogen mineralization metabolic pathways. Genes associated with nitrogen assimilation and mineralization accounted for 34-41% and 32-40% of the annotated reads related to nitrogen cycling during composting, respectively, representing them as the most abundant nitrogen metabolism processes. The gudB and norB were identified as key genes for nitrogen mineralization and nitrous oxide emission, respectively. This research offers a better understanding of the effects of additional nitrogen sources on nitrogen metabolism pathways during composting.

Cell therapy in end-stage liver disease: replace and remodel.

Liver disease is prevalent worldwide. When it reaches the end stage, mortality rises to 50% or more. Although liver transplantation has emerged as the most efficient treatment for end-stage liver disease, its application has been limited by the scarcity of donor livers. The lack of acceptable donor organs implies that patients are at high risk while waiting for suitable livers. In this scenario, cell therapy has emerged as a promising treatment approach. Most of the time, transplanted cells can replace host hepatocytes and remodel the hepatic microenvironment. For instance, hepatocytes derived from donor livers or stem cells colonize and proliferate in the liver, can replace host hepatocytes, and restore liver function. Other cellular therapy candidates, such as macrophages and mesenchymal stem cells, can remodel the hepatic microenvironment, thereby repairing the damaged liver. In recent years, cell therapy has transitioned from animal research to early human studies. In this review, we will discuss cell therapy in end-stage liver disease treatment, especially focusing on various cell types utilized for cell transplantation, and elucidate the processes involved. Furthermore, we will also summarize the practical obstacles of cell therapy and offer potential solutions.

Effect of hydrochar on antibiotic-resistance genes and relevant mechanisms during chicken manure composting.

The reduction of enhanced antibiotic resistance genes (ARGs) in compost is important to mitigate the risk of ARG transmission in agricultural production. Hydrochar is used in many applications as a functional carbon material with adsorption and catalytic properties. This study investigated the effects of hydrochar addition on bacterial communities, mobile genetic elements (MGEs), and ARGs in chicken manure composting. The addition of 2%, 5%, and 10% hydrochar (dry weight) reduced the total numbers of target ARGs and MGEs in the compost products by 40.13-55.33% and 23.63-37.23%, respectively. Hydrochar changed the succession of the bacterial population during composting, lowering the abundance of potential pathogens and promoting microbial activity in amino acid and carbohydrate metabolism. A significant possible microbial host for ARGs was found to be Firmicutes. Hydrochar was found to affect the host microorganisms and MGEs directly by altering environmental factors that indirectly impacted the ARG profiles, as shown by partial least squares pathway modeling analysis. In conclusion, the addition of hydrochar to compost is a simple and effective method to promote the removal of ARGs.

Energy and nutrient recovery by spent mushroom substrate-assisted hydrothermal carbonization of sewage sludge.

Hydrothermal carbonization (HTC) has been recognized as a promising sewage sludge (SS) treatment technology for effective pathogen elimination, bioenergy recovery, organic contaminant destruction and volume reduction. However, the solid product (hydrochar) of SS after HTC as fuel has the problems of high ash content, high nitrogen content and low calorific value. The aqueous phase (AP) produced is still considered a burden and has become a bottleneck in the development of HTC. In this study, co-HTC of SS with spent mushroom substrate (SMS) is conducted, and the fuel properties of hydrochar and the quality of the AP as a liquid fertilizer are investigated. In comparison with hydrochar of single SS, the energy yield and higher heating value of the hydrochar from co-HTC were significantly increased by 12.1-44.8 % and 33.2-137.8 %, respectively, reaching their maximum of 72.75 % and14.98 MJ/kg, respectively. Co-HTC can improve safe handling, storage and transportation, and combustion performance of hydrochar. Furthermore, the AP of co-HTC could significantly increase the biomass of pakchoi, which was 140.9 % and 90.7 % of AP from single SS and Hoagland nutrition solution (represents commercial fertilizer), respectively. The AP of co-HTC as fertilizer can recover 62.03-64.65 % nitrogen from SS and SMS. These findings suggest that co-HTC of SMS with SS is a promising technology for the conversion SS into renewable resource products for fuels and N-rich liquid fertilizer while also significantly improving fuel and fertilizer quality.

Nitrogen loss, nitrogen functional genes, and humification as affected by hydrochar addition during chicken manure composting.

This study investigated the effect of hydrochar addition on nitrogen (N) transformation, N functional genes, and humification during chicken manure composting. The addition of 10 % hydrochar reduced cumulative ammonia (NH3) and nitrous oxide emissions by 55.24 % and 45.30 %, respectively, and N losses by 32.07 %. Further, it increased the relative abundance of amoA while decreasing that of nirK, nirS, and nosZ in compost. Hydrochar reduces NH3 emissions during composting owing to its acid-carbon properties that lower the pH of the composting pile and promote ammonia oxidation. Moreover, hydrochar addition enhances the humification of the composting pile and significantly increases the content of humic substances. Moreover, after hydrochar addition, the germination index of the compost product reached >80 % 10 days earlier. The results demonstrate that hydrochar is a suitable composting additive for reducing N loss and shortening the composting time.

Emerging Diamond Quantum Sensing in Bio-Membranes.

Bio-membranes exhibit complex but unique mechanical properties as communicative regulators in various physiological and pathological processes. Exposed to a dynamic micro-environment, bio-membranes can be seen as an intricate and delicate system. The systematical modeling and detection of their local physical properties are often difficult to achieve, both quantitatively and precisely. The recent emerging diamonds hosting quantum defects (i.e., nitrogen-vacancy (NV) center) demonstrate intriguing optical and spin properties, together with their outstanding photostability and biocompatibility, rendering them ideal candidates for biological applications. Notably, the extraordinary spin-based sensing enable the measurements of localized nanoscale physical quantities such as magnetic fields, electrical fields, temperature, and strain. These nanoscale signals can be optically read out precisely by simple optical microscopy systems. Given these exclusive properties, NV-center-based quantum sensors can be widely applied in exploring bio-membrane-related features and the communicative chemical reaction processes. This review mainly focuses on NV-based quantum sensing in bio-membrane fields. The attempts of applying NV-based quantum sensors in bio-membranes to investigate diverse physical and chemical events such as membrane elasticity, phase change, nanoscale bio-physical signals, and free radical formation are fully overviewed. We also discuss the challenges and future directions of this novel technology to be utilized in bio-membranes.

Fabrication of Functional Polycatechol Nanoparticles.

While low-dimensional (1D and 2D) polycatechol materials have been widely described for a range of biomedical and surface engineering applications, very few examples have been explored that focus on the construction of functional polycatechol nanoparticles. Herein, we report the facile fabrication of a series of polycatechol nanoparticles via a general and robust strategy based on the one-step oxidation reaction. IO3--induced catechol redox chemistry could facilitate the precise size control of the resulting nanoparticles and also allow the successful transfer and amplification of microscopic monomer function into macroscopic polycatechol material properties. The ease, facileness, and controllability of such a one-step fabrication process could highly promote the development of polycatechol nanomaterials for various applications.

Foe to Friend: Supramolecular Nanomedicines Consisting of Natural Polyphenols and Bortezomib.

Bortezomib (BTZ) is a first-in-class boronate proteasome inhibitor used for cancer therapy, but its therapeutic efficacy is usually inhibited by dietary polyphenols due to boronate-catechol complexation. Benefiting from such dynamic covalent chemistry, herein we describe a novel class of supramolecular nanomedicines by rationally converting natural polyphenols from foe to friend through polyphenol-mediated BTZ assembly strategy. The simple conjugation of BTZ to catechol-containing natural polyphenols via boronate ester bond allows the facile formation of dynamic drug amphiphiles, with pH-dependent assembly/disassembly behaviors under different physiological conditions. Ferric ion was also incorporated into the supramolecular system via metal-phenolic coordination interaction to both introduce bioimaging function and facilitate stability of the supramolecular nanomedicines. Our investigation revealed that the supramolecular nanomedicine consisting of natural polyphenol, BTZ and ferric ion dramatically induced apoptosis on cancer cells and suppressed tumor growth in both subcutaneous and bone tumor models with limited adverse effects. Such natural polyphenol-mediated small drug assembly strategy enables the robust fabrication of supramolecular nanomedicines for efficient delivery and controlled release of BTZ in targeted tumor sites, which could be further employed in other types of boronic acid-containing supramolecular therapeutics toward a wide range of diseases.

Optical Properties of Oilfield Wastewater and its Application in Measuring Oil Content.

Optical properties of oilfield wastewater play an important role in the on-line measurement of oil content. As an important parameter of optical properties, absorption coefficient is usually obtained by indirectly modeling transmittance spectra. In this work, transmittance spectra of oilfield wastewater in the wavelength range of 190-900 nm at normal incidence were measured by TU-1900 double beam ultraviolet visible spectrophotometer. The absorption coefficient of oilfield wastewater was obtained by a double thickness method, and the relationship between the oil content in oilfield wastewater and its absorption coefficient was studied. The results show that the transmittance spectra of oilfield wastewater decrease with the increase of oil content. The oil content of oilfield wastewater is found to correlate negatively with its transmittance. The oil content of oilfield wastewater and its absorption coefficient have a good fitting effect at 234 nm, and the fitting error of three order polynomial fitting is minimal, with a range of 0.02-5.39%, and the fitting accuracy is 0.9940.