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1.
Sensors (Basel) ; 20(9)2020 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-32392724

RESUMO

This paper describes a novel sounding system for which the functions of the medium frequency (MF) radar and the ionosonde are integrated on the same hardware platform and antenna structure, namely the middle atmosphere-ionosphere (MAI) system. Unlike the common MF radar, MAI system adopts the pseudo-random (PRN) phase-coded modulation technology, which breaks the limitation of the traditional monopulse mode. Through the pulse compression, only a small peak power is needed to achieve the signal-to-noise ratio (SNR) requirement. The excellent anti-jamming performance is also very suitable for the ionospheric sounding. One transmitting and six receiving modes are adopted for the MF sounding. While neglecting the structure of the T/R switches, the coupling interference between the transmitter and the receiver may also be avoided. Moreover, by employing a miniaturized antenna array composed of progressive-wave antennas for the MF receiving and ionospheric sounding, the MAI system takes account of the requirements of the inversion algorithms of MF radar and the large bandwidth need for the ionospheric sounding concurrently. Such an antenna structure can also greatly simplify the system structure and minimize the difficulty of deployment. The experiments verified the availability of the system scheme and its engineering application significance. Through further analysis of the sounding data, the wind field of the mesosphere, the electron density of D layer and electron density profile from layers E to F were obtained at the identical location. The capability of MAI system can play an important role in studying the interaction and coupling mechanism between the mesosphere and ionosphere.

2.
Sensors (Basel) ; 19(4)2019 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-30769811

RESUMO

In this paper, complete complementary code (CCC) sequences are applied to a High Frequency (HF) ionospheric sounding network. Ionosondes distributed at multiple locations use the mutually orthogonal CCC sequences to conduct vertical soundings synchronously. At the same time, thanks to the omnidirectional antennas, every station can receive the oblique echoes transmitted from the others. Due to the orthogonality between the code sequences, both vertical and oblique ionograms can be simultaneously obtained and completely separated. Through this method, the sounding efficiency can be enhanced, and the inversion difficulty can be reduced. Further, by using the data assimilation method, vertical and oblique sounding results can be combined to obtain a wide range of regional ionospheric characteristics. To verify the performance of this kind of sounding network, validation experiments are implemented to demonstrate that vertical and oblique ionograms can be obtained independently at the same time and inverted separately and that the maps of foF2 parameters obtained by using the data assimilation method provide more details than single vertical or oblique sounding.

3.
Bioelectrochemistry ; 81(2): 59-64, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21458390

RESUMO

A novel electrochemical immunosensor built on three dimensional carbon nanotube-conducting polymer (CNT-CP) network is reported for detection of Hepatitis B surface antigen (HBsAg) in human serum. The CNT-CP network is prepared by drop-drying of CNT solution on glassy carbon electrode, followed by electrochemical polymerization of poly (pyrrole propionic acid) (pPPA) film to crosslink and stabilize the CNTs, wherein the CNTs form the backbone of the network, and offer great specific surface areas for antibody attachment, and confer good conductivity for electrochemical detection, while the conducting film integrates the carbon nanotubes into a stable network due to its self-limiting growth behavior and provides abundant carboxyl groups for covalent immobilization of probe proteins. As a unique matrix, the CNT-CP network enables sensitive electrochemical detection of HBsAg biomarker by using alkaline phosphatase (ALP)-conjugated secondary antibodies under sandwich format coupling with the ALP substrate solution, p-aminophenyl phosphate (PAPP), reaching a detection limit of 0.01ng/mL with a dynamic range of 5 orders of magnitude.


Assuntos
Técnicas Eletroquímicas/métodos , Antígenos de Superfície da Hepatite B/sangue , Imunoensaio/métodos , Nanotubos de Carbono/química , Polímeros/química , Fosfatase Alcalina/química , Anticorpos/química , Anticorpos/imunologia , Técnicas Biossensoriais/métodos , Eletrodos , Enzimas Imobilizadas/química , Antígenos de Superfície da Hepatite B/imunologia , Humanos , Nanotubos de Carbono/ultraestrutura , Polimerização , Sensibilidade e Especificidade , Soro/química
4.
J Biomed Opt ; 13(1): 014019, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18315377

RESUMO

The safety of tooth bleaching, which is based upon hydrogen peroxide (HP) as the active agent, has been questioned. Our aim was to investigate the effects of 30% HP on human tooth enamel. The specimens were divided randomly into three groups and treated with distilled water, HCl, and HP, respectively. Raman scattering and laser-induced fluorescence of enamel were determined before and after treatment. Microhardness testing and scanning electron microscopy were also used. The results of Raman scattering showed that the Raman relative intensity of enamel changed significantly after HP and HCl treatment. These findings were consistent with the results of microhardness testing and morphological observations. In addition, a small band at 876 cm(-1) due to O-O stretching of HP became pronounced during HP treatment, which provided direct evidence that HP has the ability to penetrate enamel. Meanwhile, the results of laser-induced fluorescence revealed that HP caused the greatest fluorescence reduction. This suggested that the organic matter in enamel might be greatly affected by HP, which was also supported by the results of microhardness. It can be concluded, therefore, that the 30% HP may have adverse effects on the mineral and the organic matter of human tooth enamel.


Assuntos
Dente Pré-Molar/fisiologia , Calcificação Fisiológica/efeitos dos fármacos , Esmalte Dentário/fisiologia , Peróxido de Hidrogênio/administração & dosagem , Medições Luminescentes/métodos , Análise Espectral Raman/métodos , Clareamento Dental/métodos , Dente Pré-Molar/efeitos dos fármacos , Calcificação Fisiológica/fisiologia , Esmalte Dentário/efeitos dos fármacos , Dureza/efeitos dos fármacos , Humanos , Peróxido de Hidrogênio/efeitos adversos , Lasers , Clareamento Dental/efeitos adversos
5.
Artigo em Inglês | MEDLINE | ID: mdl-17567528

RESUMO

Thirty-two samples from the human gastric mucosa tissue, including 13 normal and 19 malignant tissue samples were measured by confocal Raman microspectroscopy. The low signal-to-background ratio spectra from human gastric mucosa tissues were obtained by this technique without any sample preparation. Raman spectral interferences include a broad featureless sloping background due to fluorescence and noise. They mask most Raman spectral feature and lead to problems with precision and quantitation of the original spectral information. A preprocessed algorithm based on wavelet analysis was used to reduce noise and eliminate background/baseline of Raman spectra. Comparing preprocessed spectra of malignant gastric mucosa tissues with those of counterpart normal ones, there were obvious spectral changes, including intensity increase at approximately 1156cm(-1) and intensity decrease at approximately 1587cm(-1). The quantitative criterion based upon the intensity ratio of the approximately 1156 and approximately 1587cm(-1) was extracted for classification of the normal and malignant gastric mucosa tissue samples. This could result in a new diagnostic method, which would assist the early diagnosis of gastric cancer.


Assuntos
Mucosa Gástrica/química , Análise Espectral Raman/métodos , Neoplasias Gástricas/química , Neoplasias Gástricas/classificação , Mucosa Gástrica/patologia , Humanos , Neoplasias Gástricas/patologia
6.
Spectrochim Acta A Mol Biomol Spectrosc ; 62(1-3): 16-21, 2005 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-16257687

RESUMO

A novel method of curve fitting based on Gaussian function, which is used to resolve the overlapped peaks, is presented in this paper. The resolution of several kinds of overlapped peaks with noise simulated by computer has been performed and discussed in details. This method has been used for resolving of the UV-vis overlapped spectrum. The results indicate that the proposed algorithm can been used to resolve overlapped spectra effectively and satisfactorily.


Assuntos
Algoritmos , Modelos Moleculares , Análise Espectral/métodos , Alcanos/química , Compostos Azo/química , Simulação por Computador , Espectrofotometria , Espectrofotometria Ultravioleta
7.
Guang Pu Xue Yu Guang Pu Fen Xi ; 25(6): 808-10, 2005 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-16201345

RESUMO

In this paper, the formation of UV absorption spectrum from difference in temperature (UVASDT) is discussed. Broadening of spectral lines might be one of the reasons for the formation of UVASDT. The effect of temperature on the broadening of spectral lines is analyzed. The Doppler SDT function is deduced, and the SDT of C60 and progesterone can be explained by it. It is indicated that the Doppler effect might be the primary reason for the formation of UVASDT of this kind of substance.


Assuntos
Efeito Doppler , Espectrofotometria Ultravioleta/métodos , Temperatura , Algoritmos , Modelos Químicos , Progesterona/química
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