Printed on-Chip Perovskite Heterostructure Arrays for Optical Switchable Logic Gates.

The use of optoelectronic devices for high-speed and low-power data transmission and computing has been considered in the next-generation logic circuits. Heterostructures, which can generate and transmit photoresponse signals dealing with different input lights, are highly desirable for optoelectronic logic gates. Here, the printed on-chip perovskite heterostructures are demonstrated to achieve optical-controlled "AND" and "OR" optoelectronic logic gates. Perovskite heterostructures are printed with a high degree of control over composition, site, and crystallization. Different regions of the printed perovskite heterostructures exhibit distinguishable photoresponse to varied wavelengths of input lights, which can be utilized to achieve optical-controlled logic functions. Correspondingly, parallel operations of the two logic gates ("AND" and "OR") by way of choosing the output electrodes under the single perovskite heterostructure. Benefiting from the uniform crystallization and strict alignment of the printed perovskite heterostructures, the integrated 3×3 pixels all exhibit 100% logic operation accuracy. Finally, optical-controlled logic gates responding to multi-wavelength light can be printed on the predesigned micro-electrodes as the on-chip integrated circuits. This printing strategy allows for integrating heterostructure-based optical and electronic devices from a unit-scale device to a system-scale device. This article is protected by copyright. All rights reserved.

Pituitary Suppression with Gonadotropin-Releasing Hormone Agonist Prior to Artificial Endometrial Preparation in Frozen-Thawed Embryo Transfer Cycles: A Systematic Review and Meta-Analysis of Different Protocols and Infertile Populations.

This study investigates the effect of GnRHa pretreatment on pregnancy outcomes in artificial endometrial preparation for frozen-thawed embryo transfer (AC-FET) cycles. A systematic review of English language studies published before 1 September 2022, was conducted, excluding conference papers and preprints. Forty-one studies involving 43,021 participants were analyzed using meta-analysis, with a sensitivity analysis ensuring result robustness. The study found that GnRHa pretreatment generally improved the clinical pregnancy rate (CPR), implantation rate (IR), and live birth rate (LBR). However, discrepancies existed between randomized controlled trials (RCTs) and observational studies; RCTs showed no significant differences in outcomes for GnRHa-treated cycles. Depot GnRHa protocols outperformed daily regimens in LBR. Extended GnRHa pretreatment (two to five cycles) significantly improved CPR and IR compared to shorter treatment. Women with polycystic ovary syndrome (PCOS) saw substantial benefits from GnRHa pretreatment, including improved CPR and LBR and reduced miscarriage rates. In contrast, no significant benefits were observed in women with regular menstruation. More rigorous research is needed to solidify these findings.

The associations of fatty acids related dietary patterns with overweight and obesity among Chinese children.

BACKGROUND: Childhood overweight and obesity is becoming an emerging face of malnutrition. The aims of this study were to develop fatty acid (FAs) related dietary patterns and explored the associations of FAs related dietary patterns with overweight and obesity among Chinese children. METHODS: An observational study was conducted on 435 children aged 4 to 7 years old in South Central China. Erythrocyte FAs composition was analyzed by gas chromatography-mass spectrometry. Diet was collected by food frequency questionnaires and dietary patterns were evaluated by reduced rank regression. The logistic regression analysis was used to exploring the association of dietary patterns with overweight and obesity. RESULTS: The prevalence of overweight, obesity, and overweight or obesity were 6.52, 4.59, and 11.11% in Chinese children, respectively. Twenty five types of FAs were detected in erythrocyte of children and four FAs related dietary patterns were identified. The dietary pattern positively correlated with n-3 PUFAs, but negatively with SFAs,was characterized by high intake of fish, shrimp, crab and shellfish, leaf-off vegetable, nuts, and tubers, which have a significantly decreased overweight risk (OR = 0.580, 95%CI: 0.375 ∼ 0.895, P = 0.014).The pattern positively strong associated with n-6 PUFAs, but negatively strong with n-3 PUFAs, had high intake of snacks, leaf-off vegetable, fresh beans, and coarse cereals, which have a significantly decreased obesity risk (OR = 0.518, 95%CI: 0.325 ∼ 0.827, P = 0.006). CONCLUSION: Four FAs related dietary patterns were identified. The dietary pattern with high intake of fish, shrimp, crab and shellfish decreased overweight risk by increasing n-3 PUFAs, and decreasing SFAs. The dietary pattern with high intake of plant food, decreased obesity risk by providing an balanced n-6/n-3 PUFAs ratio.

Perovskite-based photodetector for real-time and quantitative monitoring of sports motion.

Reliable monitoring the movement amplitude and dynamics during sports exercise is significant for improving training results and preventing training wound. Here, we present a printed perovskite-based photodetector for real-time and quantitative monitoring of sports motion. The ordered nucleation and growth of perovskite crystals are regulated by the 4-acetamidothiophenol (AMTP) at the interface, which promotes the size of perovskite crystals into the micrometer. Benefiting from the uniformity of the AMTP-regulated MAPbI3, the as-prepared photodetector gives great photocurrent response under indoor light or outdoor light. During the exercise, real-time monitoring sports motion is achieved through detecting the illumination changing of photodetectors attaching on the wrist and ankles. Moreover, twelve kinds of common sports can be quantitatively analyzed with the detection of illumination changing on the photodetector. Such photodetector provides an efficient measurement method of wearable electronics for sports monitoring.

MiR-30a inhibits silica dust-induced epithelial-mesenchymal transition by targeting Snail.

The mechanism of action of MicroRNA-30a(miR-30a) and Snail, a transcription factor, in silica(SiO2) dust-induced pulmonary EMT and secondary pulmonary fibrosis remains elusive. In this study, the cellular EMT model induced by the stimulation of A549 cells with SiO2 was established. A549 cells were transfected with miR-30a mimic and miR-30a inhibitor and the SNAIL gene was silenced to examine the mechanism of miR-30a targeting Snail to regulate silica dust-induced EMT. The results showed that 50 µg/mL SiO2 stained A549 cells for 24 h could induce EMT in A549 cells. Exposure of A549 cells to SiO2 dust decreased miR-30a expression, as well as mRNA and protein expression levels of E-cad. Conversely, SiO2 exposure increased mRNA and protein expression levels of α-SMA, vimentin, and Snail. The miR-30a mimic upregulated mRNA and protein expression levels of E-cadherin in SiO2-induced A549 cells, while downregulating mRNA and protein expression levels of α-SMA, vimentin and Snail. MiR-30a inhibitors have the opposite effect. Silencing the SNAIL gene, followed by SiO2 dust-induced stimulation of A549 cells, could enhance mRNA and protein expression levels of E-cad, whereas those of α-SMA and vimentin were reduced. Altogether, we found that miR-30a directly targeted Snail and inhibited its expression, thereby delaying silica induced pulmonary EMT.

The relationship between dietary patterns and blood mineral concentration among children in Hunan Province of China.

BACKGROUND: Minerals have crucial biological functions in metabolism and are primarily obtained through diet. As a result, various dietary patterns can impact blood mineral levels. The aim of this study was to investigate the correlation between dietary patterns and the concentration of calcium, magnesium, iron, zinc, and copper in the bloodstream. METHODS: Three hundred eighty healthy children (53.7% male) were recruited in a region of Hunan Province in September 2019. We gathered basic information and measured physical proportions, along with completing a food frequency questionnaire (FFQ). Using principal component analysis (PCA), we determined dietary patterns. To analyze mineral levels in the blood, we used flame atomic absorption spectrometry (FAAS). We utilized linear regression models to investigate if certain dietary patterns are related to mineral concentration. RESULTS: Three dietary patterns were identified: 'Vegetables/Nuts,' 'Snacks/Beverages,' and 'Cereal/Beans.' Children from high-income families (annual average income > 50,000 yuan) prefer the 'Vegetables/Nuts' dietary pattern (P = 0.004). In comparison, those from low-income families (annual average income < 20,000 yuan) prefer the 'Snacks/Beverages' dietary pattern (P = 0.03). Following adjustment for age, gender, guardian's identity, education level, and annual household income. We found that an increase in the 'Vegetables/Nuts' pattern score (ß = 0.153, CI: 0.053 ~ 0.253; P = 0.003) and 'Snacks/Beverages' pattern score (ß = 0.103, CI: 0.002 ~ 0.204; P = 0.033) were significantly associated blood copper concentration. CONCLUSIONS: Household income was found to be associated with dietary behavior. Furthermore, higher blood copper concentration was significantly correlated with the 'Vegetables/Nuts' dietary pattern and 'Snacks/Beverages' dietary pattern, but the correlation is extremely low.

At-Home COVID-19 Rapid Antigen Test Down to 0.03 pg mL-1 of Nucleocapsid Protein.

Rapid and ultra-sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for early screening and management of COVID-19. Currently, the real-time reverse transcription polymerase chain reaction (rRT-PCR) is the primary laboratory method for diagnosing SARS-CoV-2. It is not suitable for at-home COVID-19 diagnostic test due to the long operating time, specific equipment, and professional procedures. Here an all-printed photonic crystal (PC) microarray with portable device for at-home COVID-19 rapid antigen test is reported. The fluorescence-enhanced effect of PC amplifies the fluorescence intensity of the labeled probe, achieving detection of nucleocapsid (N-) protein down to 0.03 pg mL-1 . A portable fluorescence intensity measurement instrument gives the result (negative or positive) by the color of the indicator within 5 s after inserting the reacted PC microarray test card. The N protein in inactivated virus samples (with cycle threshold values of 26.6-40.0) can be detected. The PC microarray provides a general and easy-to-use method for the timely monitoring and eventual control of the global coronavirus pandemic.

Macrophage migration inhibitory factor facilitates astrocytic production of the CCL2 chemokine following spinal cord injury.

Spinal cord injury causes accumulation of a large number of leukocytes at the lesion site where they contribute to excessive inflammation. Overproduced chemokines are responsible for the migratory process of the leukocytes, but the regulatory mechanism underlying the production of chemokines from resident cells of the spinal cord has not been fully elucidated. We examined the protein levels of macrophage migration inhibitory factor and chemokine C-C motif chemokine ligand 2 in a spinal cord contusion model at different time points following spinal cord injury. The elevation of macrophage migration inhibitory factor at the lesion site coincided with the increase of chemokine C-C motif chemokine ligand 2 abundance in astrocytes. Stimulation of primary cultured astrocytes with different concentrations of macrophage migration inhibitory factor recombinant protein induced chemokine C-C motif chemokine ligand 2 production from the cells, and the macrophage migration inhibitory factor inhibitor 4-iodo-6-phenylpyrimidine attenuated the stimulatory effect. Further investigation into the underlying mechanism on macrophage migration inhibitory factor-mediated astrocytic production of chemokine C-C motif chemokine ligand 2 revealed that macrophage migration inhibitory factor activated intracellular JNK signaling through binding with CD74 receptor. Administration of the macrophage migration inhibitory factor inhibitor 4-iodo-6-phenylpyrimidine following spinal cord injury resulted in the reduction of chemokine C-C motif chemokine ligand 2-recruited microglia/macrophages at the lesion site and remarkably improved the hindlimb locomotor function of rats. Our results have provided insights into the functions of astrocyte-activated chemokines in the recruitment of leukocytes and may be beneficial to develop interventions targeting chemokine C-C motif chemokine ligand 2 for neuroinflammation after spinal cord injury.

Cervical Secretion Methylation Is Associated with the Pregnancy Outcome of Frozen-Thawed Embryo Transfer.

The causes of implantation failure remain a black box in reproductive medicine. The exact mechanism behind the regulation of endometrial receptivity is still unknown. Epigenetic modifications influence gene expression patterns and may alter the receptivity of human endometrium. Cervical secretions contain endometrial genetic material, which can be used as an indicator of the endometrial condition. This study evaluates the association between the cervical secretion gene methylation profile and pregnancy outcome in a frozen-thawed embryonic transfer (FET) cycle. Cervical secretions were collected from women who entered the FET cycle with a blastocyst transfer (36 pregnant and 36 non-pregnant women). The DNA methylation profiles of six candidate genes selected from the literature review were measured by quantitative methylation-specific PCR (qMSP). Bioinformatic analysis of six selected candidate genes showed significant differences in DNA methylation between receptive and pre-receptive endometrium. All candidate genes showed different degrees of correlation with the pregnancy outcomes in the logistic regression model. A machine learning approach showed that the combination of candidate genes' DNA methylation profiles could differentiate pregnant from non-pregnant samples with an accuracy as high as 86.67% and an AUC of 0.81. This study demonstrated the association between cervical secretion methylation profiles and pregnancy outcomes in an FET cycle and provides a basis for potential clinical application as a non-invasive method for implantation prediction.

Thrombin increases the expression of cholesterol 25-hydroxylase in rat astrocytes after spinal cord injury.

Astrocytes are important cellular centers of cholesterol synthesis and metabolism that help maintain normal physiological function at the organism level. Spinal cord injury results in aberrant cholesterol metabolism by astrocytes and excessive production of oxysterols, which have profound effects on neuropathology. 25-Hydroxycholesterol (25-HC), the main product of the membrane-associated enzyme cholesterol-25-hydroxylase (CH25H), plays important roles in mediating neuroinflammation. However, whether the abnormal astrocyte cholesterol metabolism induced by spinal cord injury contributes to the production of 25-HC, as well as the resulting pathological effects, remain unclear. In the present study, spinal cord injury-induced activation of thrombin was found to increase astrocyte CH25H expression. A protease-activated receptor 1 inhibitor was able to attenuate this effect in vitro and in vivo. In cultured primary astrocytes, thrombin interacted with protease-activated receptor 1, mainly through activation of the mitogen-activated protein kinase/nuclear factor-kappa B signaling pathway. Conditioned culture medium from astrocytes in which ch25h expression had been knocked down by siRNA reduced macrophage migration. Finally, injection of the protease activated receptor 1 inhibitor SCH79797 into rat neural sheaths following spinal cord injury reduced migration of microglia/macrophages to the injured site and largely restored motor function. Our results demonstrate a novel regulatory mechanism for thrombin-regulated cholesterol metabolism in astrocytes that could be used to develop anti-inflammatory drugs to treat patients with spinal cord injury.

D-dopachrome tautomerase drives astroglial inflammation via NF-κB signaling following spinal cord injury.

BACKGROUND: Reactive astrocytes are increasingly recognized as crucial regulators of innate immunity in degenerative or damaged central nervous system (CNS). Many proinflammatory mediators have been shown to drive inflammatory cascades of astrocytes through activation of NF-κB, thereby affecting the functional outcome of the insulted CNS. D-dopachrome tautomerase (D-DT), a newly described cytokine and a close homolog of proinflammatory macrophage migration inhibitory factor (MIF), has been revealed to share receptor and overlapping functional spectrum with MIF, but little is known about its roles in the neuropathological progression of the CNS and relevant regulatory mechanisms. RESULTS: D-DT protein levels were significantly elevated within neurons and astrocytes following SCI. Analysis of transcriptome profile revealed that D-DT was able to activate multiple signal pathways of astrocytes, which converged to NF-κB, a hub regulator governing proinflammatory response. Rat D-DT recombinant protein was efficient in inducing the production of inflammatory cytokines from astrocytes through interaction with CD74 receptor. Activation of mitogen-activated protein kinases (MAPKs) and NF-κB was observed to be essential for the transduction of D-DT signaling. Administration of D-DT specific inhibitor at lesion sites of the cord resulted in significant attenuation of NF-κB activation and reduction of the inflammatory cytokines following SCI, and accordingly improved the recovery of locomotor functions. CONCLUSION: Collectively, D-DT is a novel proinflammatory mediator of astrocytes following SCI. Insights of its cell-specific expression and relevant proinflammatory mechanisms will provide clues for the control of CNS inflammation.

Photosynthesis and growth of Amaranthus tricolor under strontium stress.

Amaranthaceae are effective plants for cleaning soil contaminated by heavy metals and radionuclides. In this paper, Amaranthus tricolor was used to investigate the response of the plant photosynthesis to various concentration of strontium ions (0.2, 0.6, 3 and 6 mM), in order to determine the possibility of A. tricolor to remediate strontium contamination. The results showed that strontium ions (0.2-6 mM) had effect on light energy conversion and utilization in A. tricolor. Low level of strontium (0.2 mM) promoted the energy utilization in A. tricolor, while higher Sr concentration (3 mM or higher) increased the excess light energy in the plants. Under strontium stress of 6 mM, the acceptor side of PSII in A. tricolor leaves was more vulnerable to strontium stress than the donor side. Furthermore, strontium stress led to accumulation of QA- and block in QB downstream of the electron transfer chain in PSII of A. tricolor leaves. The tolerance ability of A. tricolor to strontium and remediation is also reflected in its biomass and strontium content in plants. Strontium at 3 mM or below promoted the growth of A. tricolor, while higher concentration inhibited the plant growth, but without obvious wilting or curling of leaves. The maximal dry weight increased by 36.29% in shoots, and 60.14% in roots when the spiked-strontium concentration reached 0.2 mM. The maximal strontium content achieved 8.75 mg/g dry wt in shoots, and 1.71 mg/g dry wt in roots respectively, when strontium concentration was 6 mM. Transfer factors (TFs: ratio of Sr content in shoots to that in roots) of strontium in A. tricolor ranged from 2.85 to 5.93, while bio-concentration factors (BCFs: ratio of Sr content in shoots to that in solutions) ranged from 22.57 to 49.66. In summary, A. tricolor showed the excellent potential to remediate strontium contamination.

Docosahexaenoic Acid as the Bidirectional Biomarker of Dietary and Metabolic Risk Patterns in Chinese Children: A Comparison with Plasma and Erythrocyte.

Objective: The present study aims to measure docosahexaenoic acid (DHA) in both the plasma and erythrocyte of a child population and compares them with respect to their associations with dietary and metabolic risk patterns. Methods: A cross-sectional study was conducted, and a total of 435 children ages 5−7 years old were recruited. Diet information was collected using a food frequency questionnaire (FFQ). The physical indicators, blood pressure, and glycolipid metabolic indicators were determined. The plasma and erythrocyte DHA were analyzed using a gas chromatography mass spectrometer. Principal component analysis was used to identify dietary and metabolic risk patterns. Multivariate regression analyses were used to investigate the associations of DHA status with dietary and metabolic risk patterns. Results: A significant correlation between plasma and the erythrocyte DHA concentration was found (r = 0.232, p < 0.001). A diversified dietary pattern characterized that a high intake of diversified foods had a positive association with the plasma DHA level (ß = 0.145, 95% CI: 0.045~0.244, p = 0.004). Children of obesity risk patterns with a high weight, pelvis breadth, BMI, upper arm circumference, and chest circumference had lower plasma DHA concentrations (OR = 0.873, 95% CI: 0.786~0.969, p = 0.011). Children with higher plasma and erythrocyte DHA concentrations were adhered to blood lipid risk patterns with high CHOL and LDL-C levels. The plasma DHA (OR = 1.271, 95% CI: 1.142~1.415, p < 0.001) had a stronger association with a blood lipid risk pattern than erythrocyte (OR = 1.043, 95% CI: 1.002~1.086, p = 0.040). Conclusions: The diversified dietary pattern had a higher plasma DHA concentration. Lower levels of plasma DHA were positively associated with obesity in children. DHA in plasma appears to be more strongly associated with blood lipid metabolism than erythrocyte. Plasma DHA may be a more sensitive bidirectional biomarker to evaluate the recently comprehensive diet intake and metabolic risk of children.

Co-fermentation of sewage sludge and lignocellulosic biomass for production of medium-chain fatty acids.

Medium-chain fatty acids (MCFAs) production from sewage sludge and lignocellulosic biomass (fallen leaves and grass) was explored. Co-fermentation of sludge and lignocellulosic biomass significantly accelerated the caproate production and promoted the longer-chain MCFAs formation. Co-fermentation of sludge and grass achieved the highest caproate production of 89.50 mmol C/L, which was 18.04 % and 41.73 % higher than the mono-fermentation of grass and sludge, respectively. Co-fermentation of sludge and leaves produced 63.80 mmol C/L caproate, which was 11.09 % and 1.03 % higher than the mono-fermentation of leaves and sludge, respectively. Microbial analysis showed that co-fermentation enriched CE microbes like genus Clostridium_sensu_stricto_13, Caprocipiproducens, Terrisporpbacter and Praraclostridium, and suppressed the competitive microbes like genus norank_f_Caldilineaceae and Desulfomicrobium. Functional enzymes analysis revealed that co-fermentation of sludge and leaves promoted MCFAs production through strengthening reverse ß oxidation (RBO) pathway, while co-fermentation of sludge and grass stimulated MCFAs production by strengthening fatty acid biosynthesis (FAB) pathway.

Response of Amaranthus tricolor to cesium stress in hydroponic system: Growth, photosynthesis and cesium accumulation.

Remediation of the cesium-contaminated environment is of paramount importance, and phytoremediation is a cost-effective and green technique. In this paper, the response of Amaranthus tricolor to cesium ions in hydroponic solution was investigated at various cesium concentration (0, 0.05, 0.2, 0.4 and 0.6 mM), in terms of the growth weight, height and photosynthesis. The maximal Cs content in stems and leaves of A. tricolor was 13.05 mg/g dry wt under spiked Cs level of 0.4 mM in solution. The maximal transfer factor (TF) and bioconcentration factor (BCF) were 1.87 and 181.25 respectively, when the corresponding Cs content in roots and shoots was 7.04 mg/g and 13.05 mg/g dry wt respectively. TFs are higher than 1 in the conditions of normal plant growth. The growth of A. tricolor was enhanced after the treatment of Cs at low concentrations (0.05 and 0.2 mM), while it was inhibited at 0.4 and 0.6 mM. The leaf number and dry weight of stem, leaf parts and root parts were maximum at the spiked cesium level of 0.2 mM, which significantly increased by 19.19%, 47.56% and 94.56% respectively, compared with the control samples. Under 0.6 mM cesium stress, curl and withering of the leaves occurred, and the plant growth and cesium accumulation dropped to the minimum. Cs at the spiked level of 0.6 mM in solution inhibited the performance of PSII, especially in terms of blockage in electron transfer process beyond QA and restraint of P700 reduction. On contrast, the performance of PSII was enhanced by the spiked Cs at level of 0.2 mM, leading to the growing density of reaction centers per excited cross-section and increasing electron transfer process beyond QA. In summary, A. tricolor has potential for remediating the Cs-contaminated environment.

Nitrate sources and its formation in precipitation during typhoons (In-fa and Chanthu) in multiple cities, East China.

A clear understanding of the factors governing dual isotopes (δ15N-NO3- and δ18O-NO3-) in typhoons is essential for understanding their NO3- sources and its formation mechanisms. In this study, sequential precipitation samples during typhoons, including In-fa and Chanthu, were collected from Ningbo, Hangzhou and Huzhou. The chemical compositions, nitrogen and oxygen isotopes of NO3- and oxygen isotopes of H2O (δ18O-H2O) were measured. The results showed that the δ15N-NO3- and δ18O-NO3- values ranged from -6.3‰ to 6.0‰, and 38.0‰ to 66.5‰, respectively. The lower δ18O-NO3- values (less than 52‰) indicated the importance of peroxy radicals (RO2 or HO2) in NOx oxidation to NO3- formation pathways. By the Monte Carlo simulation of δ18O-NO3- values of typhoons, the calculated oxidation proportions of NO by RO2 (or HO2) during the OH· pathway ranged from 0% to 27% of In-fa and from 0% to 32% of Chanthu, respectively, in the three cities. More NOx emissions from marine microbial processes caused the lower δ15N-NO3- values of typhoons in Ningbo than those in Hangzhou and Huzhou. The variation in δ15N-NO3- values in sequential samples in In-fa reflected the decreased marine sources (lightning) and the increased anthropogenic sources in land (coal combustion and microbial N cycle) from Phrase I to Phrase II and III. Based on the improved Bayesian model with nitrogen isotopic fractionation, the contributions of lightning + biomass burning, coal combustion, mobile sources and the microbial N cycle were 35.7%, 22.5%, 27.1% and 14.7% in In-fa, and 28.3%, 32.3%, 28.0% and 11.4% in Chanthu, respectively, in the three cities, emphasizing the influence of marine NOx sources (lightning). The results highlight the importance of RO2 (or HO2) in NOx oxidation pathways in typhoons and provide valuable insight into the NOx sources of typhoons.

Genome-wide analysis of cervical secretions obtained during embryo transfer reveals the association between deoxyribonucleic acid methylation and pregnancy outcomes.

OBJECTIVE: To study whether the methylation status of cervical secretions can reflect the ability of the endometrium to allow embryo implantation. DESIGN: Case-control study. SETTING: In vitro fertilization centers. PATIENT(S): Women undergoing embryo transfer cycles, in which at least 1 good-quality embryo was transferred. INTERVENTION(S): Collection of cervical secretions during the procedure of embryo transfer. MAIN OUTCOME MEASURE(S): Methylation profiles of cervical secretions in relation to pregnancy outcomes. RESULT(S): Genome-wide methylation profiles differ between cervical secretions from pregnancy and nonpregnancy cycles. Clustering analysis on the basis of the top 2,000 differentially methylated probes of cervical secretions from 28 pregnancy and 29 nonpregnancy cycles correctly categorized 86.0% of the samples in terms of conceptional status, which was verified in selected genes by quantitative methylation-specific polymerase chain reaction and validated in another independent sample set. The combination of selected genes was estimated to predict pregnancy outcomes with a maximal area under the receiver operating characteristic curve of 0.83. CONCLUSION(S): The methylation profiles of cervical secretions were associated with pregnancy outcomes in embryo transfer cycles. Although not clinically useful at present, deoxyribonucleic acid methylation in cervical secretions may shed new light on the less invasive assessment of endometrial receptivity.

Thrombin induces morphological and inflammatory astrocytic responses via activation of PAR1 receptor.

Spinal cord injury (SCI) will result in the significant elevation of thrombin production at lesion site via either breakage of blood-spinal cord barrier or upregulated expression within nerve cells. Thrombin-induced activation of the protease activated receptors (PARs) evokes various pathological effects that deteriorate the functional outcomes of the injured cord. The cellular consequences of thrombin action on the astrocytes, as well as the underlying mechanism are not fully elucidated by far. In the present study, SCI model of rats was established by contusion, and primary astrocytes were isolated for culture from newborn rats. The expression levels of thrombin and PAR1 receptor at lesion sites of the spinal cord were determined. The primary astrocytes cultured in vitro were stimulated with different concentration of thrombin, and the resultant morphological changes, inflammatory astrocytic responses, as well as PAR1-activated signal pathway of astrocytes were accordingly examined using various agonists or antagonists of the receptor. Thrombin was found to reverse astrocytic stellation, promote proliferation but inhibit migration of astrocytes. Furthermore, the serine protease was shown to facilitate inflammatory response of astrocytes through regulation of MAPKs/NFκB pathway. Our results have provided the morphological evidence of astrocytic reactivity in response to thrombin stimulation and its neuroinflammatory effects following SCI, which will be indicative for the fundamental insights of thrombin-induced neuropathology.

The Associations of Erythrocyte Fatty Acids with Whole Blood Mineral Elements in Children.

Background. Minerals play important biological roles in lipid metabolism. The primary aim of this study was to examine the relationships between erythrocyte fatty acids (FAs) levels with whole blood mineral elements concentrations among Chinese children. Methods. A cross-sectional study was conducted. A total of 435 children aged 4−7 years were recruited. Whole blood mineral elements were determined by atomic absorption spectrometry and erythrocyte FAs composition by gas chromatography-mass spectrometer. Results. There were direct correlations between Zn and C18:2n-6 (FDR corrected p = 0.019), total n-6 PUFAs (FDR corrected p = 0.034), and total PUFAs (FDR corrected p = 0.034). Direct correlations were found between whole blood Zn and C18:1n-9 (FDR corrected p = 0.035), C24:1n-9 (FDR corrected p = 0.023), total MUFAs (FDR corrected p = 0.023), and C18:2n-6 (FDR corrected p = 0.048) in the Cu < P50 group. In the Cu ≥ P50 group, Mg was inversely related to most FAs (All FDR corrected p < 0.05). In the Zn < P50 group, Cu was directly related to C24:1n-9, total MUFAs, C20:5n-3, C22:6n-3, total n-3 PUFAs, C20:4n-6, total n-6 PUFAs, total PUFAs, and total FAs (All FDR corrected p < 0.05). Conclusions. Whole blood Cu and Zn levels were directly linked to several FAs levels in the erythrocytes of children. The interactions of Mg, Cu, and Zn with fatty acids may affect FA metabolism, in which Mg influences FA absorption.