Urinary SPP1 has potential as a non-invasive diagnostic marker for focal segmental glomerulosclerosis.

Focal segmental glomerulosclerosis (FSGS) is a type of chronic glomerular nephropathy showing characteristic glomerular sclerosis, diagnosed by kidney biopsy. However, it is difficult and expensive to monitor disease progression with repeated renal biopsy in clinical practice, and thus here we explored the feasibility of urine biomarkers as non-invasive diagnostic tools. We downloaded scRNA-seq datasets of 20 urine cell samples and 3 kidney tissues and obtained two gene lists encoding extracellular proteins for bioinformatic analysis; in addition, we identified key EP-Genes by immunohistochemical staining and performed bulk RNA sequencing with 12 urine samples. We report that urine cells and kidney cells were correlated. A total of 64 EP-Genes were acquired by intersecting genes of distal tubular cluster with extracellular proteins. Function enrichment analysis showed that EP-Genes might be involved in the immune response and extracellular components. Six key EP-Genes were identified and correlated with renal function. IMC showed that key EP-Genes were located mainly in tubules. Cross verification and examination of a urine RNAseq dataset showed that SPP1 had diagnostic potential for FSGS. The presence of urine SPP1 was primarily associated with macrophage infiltration in kidney, and the pathogenesis of FSGS may be related to innate immunity. Urinary cells seemed to be strongly similar to kidney cells. In summary, SPP1 levels reflect renal function and may have potential as a biomarker for non-invasive diagnosis of FSGS.

Infant food production environments: A potential reservoir for vancomycin-resistant enterococci non-nosocomial infections.

Enterococcus has been considered one of the most important nosocomial pathogens for human infections, and the hospital environment is an important reservoir for vancomycin-resistant enterococci (VRE) that leads to antimicrobial therapeutic failure. However, infant foods and their production environments could pose risks for the immature population, while this question remains unaddressed. This study conducted an extensive and thorough Enterococcus isolation, VRE risk assessment of the Chinese infant food production chains and additional online-marketing infant foods, including powdered infant formula (PIF) and infant complementary food (ICF). To investigate the prevalence of Enterococcus along infant food chains and commodities, a total of 482 strains of Enterococcus, including E. faecium (n = 363), E. faecalis (n = 84), E. casseliflavus (n = 13), E. mundtii (n = 12), E. gallinarum (n = 4), E. hirae (n = 4), and E. durans (n = 2) were recovered from 459 samples collected from infant food production chains (71/254) and food commodities (67/205). A decreasing trend for Enterococcus detection rate was found in the PIF production chain (PIF-PC), particularly during the preparation of the PIF base powder (From 100 % in raw milk to 8.70 % in end products), while an increasing trend was observed in the ICF production chain (ICF-PC) mainly during the initial processing of farm crops and the further processing of the product (20 % at farm crops increasing to 76.92 % at end products). The result indicated that the PIF-PC process effectively reduced Enterococcus contamination, while the ICF-PC showed the opposite trend. Importantly, eleven VRE isolates were recovered from the infant food production chain, including seven E. casseliflavus isolates carrying vanC2/C3 and four E. gallinarum isolates carrying vanC1. Ten VRE isolates were from food production environments. Collectively, our study demonstrated that infant food production environments represent potential reservoirs for VRE non-nosocomial infections in vulnerable populations.

Urinary PART1 and PLA2R1 Could Potentially Serve as Diagnostic Markers for Diabetic Kidney Disease Patients.

Background: Diabetic kidney disease (DKD) is a chronic renal disease which could eventually develop into renal failure. Though albuminuria and estimated glomerular filtration rate (eGFR) are helpful for the diagnosis of DKD, the lack of specific biomarkers reduces the efficiency of therapeutic interventions. Methods: Based on bulk-seq of 56 urine samples collected at different time points (including 11 acquired from DKD patients and 11 from healthy controls), in corporation of scRNA-seq data of urine samples and snRNA-seq data of renal punctures from DKD patients (retrieved from NCBI GEO Omnibus), urine-kidney specific genes were identified by Multiple Biological Information methods. Results: Forty urine-kidney specific genes/differentially expressed genes (DEGs) were identified to be highly related to kidney injury and proteinuria for the DKD patients. Most of these genes participate in regulating glucagon and apoptosis, among which, urinary PART1 (mainly derived from distal tubular cells) and PLA2R1 (podocyte cell surface marker) could be used together for the early diagnosis of DKD. Moreover, urinary PART1 was significantly associated with multiple clinical indicators, and remained stable over time in urine. Conclusion: Urinary PART1 and PLA2R1 could be shed lights on the discovery and development of non-invasive diagnostic method for DKD, especially in early stages.

Sucrose Facilitates Rhizome Development of Perennial Rice (Oryza longistaminata).

Compared with annual crops, perennial crops with longer growing seasons and deeper root systems can fix more sunlight energy, and have advantages in reducing soil erosion and saving water, fertilizer and pesticide inputs. Rice is one of the most important food crops in the world. Perennial rice can be of great significance for protecting the ecological environment and coping with the shortage of young farmers due to urbanization. Oryza longistaminata (OL) is a rhizomatous wild rice with an AA genome and has strong biotic and abiotic resistances. The AA genome makes OL easy to cross with cultivated rice, thus making it an ideal donor material for perennial rice breeding. Sucrose plays an important role in the development and growth of plants. In this study, OL seedlings were cultured in medium with different concentrations of sucrose, and it was found that sucrose of appropriate concentrations can promote the sprout of basal axillary buds and the subsequent development of rhizomes. In order to explore the molecular mechanism, comparative transcriptome analysis was carried out with OL cultured under two concentrations of sucrose, 20 g/L and 100 g/L, respectively. The results showed that the boost of sucrose to rhizome elongation may be due to the glucose and fructose, hydrolyzed from the absorbed sucrose by vacuolar acid invertase. In addition, the consequent increased osmotic pressure of the cells would promote water absorption, which is benefit for the cell elongation, eventually causing the rhizome elongation. These results may provide a reference for elucidating the regulatory mechanism of sucrose on the rhizome development of OL.

Inhibiting pannexin-1 alleviates sepsis-induced acute kidney injury via decreasing NLRP3 inflammasome activation and cell apoptosis.

AIMS: Sepsis-induced acute kidney injury (SI-AKI) is the fifth most common cause of hospital-acquired acute kidney injury. Pannexin1 (Panx1) triggers inflammation and apoptosis which act as crucial factors in the pathogenesis of SI-AKI. We aimed to investigate the expression of Panx1 and its role on the inflammation and apoptosis in SI-AKI. MATERIALS AND METHODS: SI-AKI model was established by lipopolysaccharide (LPS) injection in mice and LPS-treated HK-2 cells in vitro. Panx1 was inhibited by pretreating with carbenoxolone (CBX) or small interfering RNA in vivo and vitro, respectively. The expression of Panx1 was determined by qPCR, western blot and immunohistochemistry (IHC). Kidney damage was evaluated by kidney function, histopathological examination and AKI biomarkers. Inflammatory cytokines were detected by qPCR and ELISA. Apoptosis was detected by TUNEL staining and the expression of apoptosis-related proteins. The activation of nucleotide-binding domain-like receptor protein 3 (NLRP3) inflammasome was measured by western blot. KEY FINDINGS: Panx1 increased in LPS-induced SI-AKI mice and HK-2 cells, as well as in SI-AKI patients. CBX alleviated the renal function and pathological damage, as well as decreased the mRNA of kidney injury molecule (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL). Inhibiting Panx1 decreased the production of IL-1ß, IL-6 and TNF-α, as well as tubular cell apoptosis in SI-AKI. Inhibiting Panx1 suppressed inflammatory cytokines and apoptosis via inhibiting NLRP3 inflammasome activation and regulating apoptotic protein Bax and Bcl2 expression, respectively. SIGNIFICANCE: These observations suggest that pharmacological inhibition of Panx1 might be a potential approach in the clinical therapy of SI-AKI.

Asynchronous RTK Method for Detecting the Stability of the Reference Station in GNSS Deformation Monitoring.

The real-time kinematic (RTK) positioning technique of global navigation satellite systems (GNSS) has been widely used for deformation monitoring in the past several decades. The RTK technique can provide relative displacements in a local reference frame defined by a highly stable reference station. However, the traditional RTK solution does not account for reference stations that experience displacement. This presents a challenge for establishing a near real-time GNSS monitoring system, as since the displacement of a reference station can be easily misinterpreted as a sign of rapid movement at the monitoring station. In this study, based on the reference observations in different time domains, asynchronous and synchronous RTK are proposed and applied together to address this issue, providing more reliable displacement information. Using the asynchronously generated time difference of a reference frame, the proposed approach can detect whether a measured displacement has occurred in the reference or the monitoring station in the current epoch. This allows for the separation of reference station movements from monitoring station movements. The results based on both simulated and landslide monitoring data demonstrate that the proposed method can provide reliable displacement determinations, which are critical in deformation monitoring applications, such as the early warning of landslides.

Characteristics and Performance Evaluation of QZSS Onboard Satellite Clocks.

In the Global Navigation Satellite System (GNSS) community, the Quasi-Zenith Satellite System (QZSS) is an augmentation system for users in the Asia-Pacific region. However, the characteristics and performance of four QZSS satellite clocks in a long-term scale are unknown at present. However, it is crucial to the positioning, navigation and timing (PNT) services of users, especially in Asia-Pacific region. In this study, the characteristics and performance variation of four QZSS satellite clocks, which including the phase, frequency, frequency drift, fitting residuals, frequency accuracy, periodic terms, frequency stability and short-term clock prediction, are revealed in detail for the first time based on the precise satellite clock offset products of nearly 1000 days. The important contributions are as follows: (1) It is detected that the times of phase and frequency jump are 2.25 and 1.5 for every QZSS satellite clock in one year. The magnitude of the frequency drift is about 10-18. The periodic oscillation of frequency drift of J01 and J02 satellite clocks is found. The clock offset model precision of QZSS is 0.33 ns. (2) The two main periods of QZSS satellite clock are 24 and 12 hours, which is the influence of the satellite orbit; (3) The frequency stability of 100, 1000 and 10,000 s are 1.98 × 10-13, 6.59 × 10-14 and 5.39 × 10-14 for QZSS satellite clock, respectively. The visible "bump" is found at about 400 s for J02 and J03 satellite clocks. The short-term clock prediction accuracy of is 0.12 ns. This study provides a reference for the state monitoring and performance variation of the QZSS satellite clock.

A New Asynchronous RTK Method to Mitigate Base Station Observation Outages.

Real-time kinematic (RTK) positioning is a satellite navigation technique that is widely used to enhance the precision of position data obtained from global navigation satellite systems (GNSS). This technique can reduce or eliminate significant correlation errors via the enhancement of the base station observation data. However, observations received by the base station are often interrupted, delayed, and/or discontinuous, and in the absence of base station observation data the corresponding positioning accuracy of a rover declines rapidly. With the strategies proposed till date, the positioning accuracy can only be maintained at the centimeter-level for a short span of time, no more than three min. To address this, a novel asynchronous RTK method (that addresses asynchronous errors) that can bridge significant gaps in the observations at the base station is proposed. First, satellite clock and orbital errors are eliminated using the products of the final precise ephemeris during post-processing or the ultra-rapid precise ephemeris during real-time processing. Then the tropospheric error is corrected using the Saastamoinen model and the asynchronous ionospheric delay is corrected using the carrier phase measurements from the rover receiver. Finally, a straightforward first-degree polynomial function is used to predict the residual asynchronous error. Experimental results demonstrate that the proposed approach can achieve centimeter-level accuracy for as long as 15 min during interruptions in both real-time and post-processing scenarios, and that the accuracy of the real-time scheme can be maintained for 15 min even when a large systematic error is projected in the U direction.

Reliable Indoor Pseudolite Positioning Based on a Robust Estimation and Partial Ambiguity Resolution Method.

The unscented Kalman filter (UKF) can effectively reduce the linearized model error and the dependence on initial coordinate values for indoor pseudolite (PL) positioning unlike the extended Kalman filter (EKF). However, PL observations are prone to various abnormalities because the indoor environment is usually complex. Standard UKF (SUKF) lacks resistance to frequent abnormal observations. This inadequacy brings difficulty in guaranteeing the accuracy and reliability of indoor PL positioning, especially for phase-based high-precision positioning. In this type of positioning, the ambiguity resolution (AR) will be difficult to achieve in the presence of abnormal observations. In this study, a robust UKF (RUKF) and partial AR (PAR) algorithm are introduced and applied in indoor PL positioning. First, the UKF is used for parameter estimation. Then, the anomaly recognition statistics and optimal ambiguity subset of PAR are constructed on the basis of the posterior residuals. The IGGIII scheme is adopted to weaken the influence of abnormal observation, and the PAR strategy is conducted in case of failure of the conventional PL-AR. The superiority of our proposed algorithm is validated using the measured indoor PL data for code-based differential PL (DPL) and phase-based real-time kinematic (RTK) positioning modes. Numerical results indicate that the positioning accuracy of RUKF-based indoor DPL is higher with a decimeter-level improvement compared that of the SUKF, especially in the presence of large gross errors. In terms of high-precision RTK positioning, RUKF can correctly identify centimeter-level anomalous observations and obtain a corresponding positioning accuracy improvement compared with the SUKF. When relatively large gross errors exist, the conventional method cannot easily realize PL-AR. By contrast, the combination of RUKF and the PAR algorithm can achieve PL-AR for the selected ambiguity subset successfully and can improve the positioning accuracy and reliability significantly. In summary, our proposed algorithm has certain resistance ability for abnormal observations. The indoor PL positioning of this algorithm outperforms that of the conventional method. Thus, the algorithm has some practical application value, especially for kinematic positioning.

A Method to Determine BeiDou GEO/IGSO Orbital Maneuver Time Periods.

Because there are different types of BeiDou constellations with participating geostationary orbit (GEO) and inclined geosynchronous orbit (IGSO) satellites, the maneuvering frequency of BeiDou satellites is higher than that of other navigation systems. The satellite orbital maneuvers lead to orbital parameter failure for several hours from broadcast ephemeris. Due to the missing initial orbit, the maneuvering thrust, and the period of orbital maneuvering, the orbit products of maneuvering satellites cannot be provided by the International Global Navigation Satellite System (GNSS) Service (IGS) and International GNSS Monitoring and Assessment System (iGMAS). In addition, the period of unhealthy status and the orbital parameters of maneuvering satellites in broadcast ephemeris are unreliable, making the detection of orbital maneuver periods more difficult. Here, we develop a method to detect orbital maneuver periods involving two key steps. The first step is orbit prediction of maneuvering satellites based on precise orbit products. The second step is time period detection of orbit maneuvering. The start time detection factor is calculated by backward prediction orbit and pseudo-range observations, and the end time detection factor is calculated by forward prediction orbit and pseudo-range observations. Data of stations from the Multi-GNSS Experiment (MGEX) and iGMAS were analyzed. The results show that the period of orbit maneuvering could be detected accurately for BeiDou GEO and IGSO satellites. In addition, the orbital maneuver period of other GNSS medium Earth orbit (MEO) satellites could also be determined by this method. The results of period detection for orbit maneuvering provide important reference information for precision orbit and clock offset determination during satellite maneuvers.

A New Azimuth-Dependent Elevation Weight (ADEW) Model for Real-Time Deformation Monitoring in Complex Environment by Multi-GNSS.

Global navigation satellite systems (GNSS) have provided an excellent way to monitor micro-deformation in real-time. However, at local sites where landslides frequently occur, the environment can include complex surroundings with mountains, dense vegetation, and human settlements, which can severely degrade the accuracy of positioning with the GNSS technique. In this study, we propose an azimuth-dependent elevation weight (ADEW) model using an azimuth-dependent elevation mask (ADEM) to reduce the effects of multipath errors and improve the accuracy of real-time deformation monitoring in such environments. We developed an adaptive fixed-elevation mask to serve as the outlier of low precision observations at lower elevations for the ADEM, and then, we applied the weighted phase observations into the mitigation process for the effects of multipath errors. The real numerical results indicate that the ADEM model performs better than the conventional model, and the average improvements were 18.91% and 34.93% in the horizontal and vertical direction, respectively. The ADEW model further improved upon the ADEM model results by an additional 21.9% and 29.8% in the horizontal and vertical direction, respectively. Therefore, we propose that the ADEW model can significantly mitigate the effects of multipath errors and improve the accuracy of micro-deformation monitoring via GNSS receivers.

An Optimized Method to Detect BDS Satellites' Orbit Maneuvering and Anomalies in Real-Time.

The orbital maneuvers of Global Navigation Satellite System (GNSS) Constellations will decrease the performance and accuracy of positioning, navigation, and timing (PNT). Because satellites in the Chinese BeiDou Navigation Satellite System (BDS) are in Geostationary Orbit (GEO) and Inclined Geosynchronous Orbit (IGSO), maneuvers occur more frequently. Also, the precise start moment of the BDS satellites' orbit maneuvering cannot be obtained by common users. This paper presented an improved real-time detecting method for BDS satellites' orbit maneuvering and anomalies with higher timeliness and higher accuracy. The main contributions to this improvement are as follows: (1) instead of the previous two-steps method, a new one-step method with higher accuracy is proposed to determine the start moment and the pseudo random noise code (PRN) of the satellite orbit maneuvering in that time; (2) BDS Medium Earth Orbit (MEO) orbital maneuvers are firstly detected according to the proposed selection strategy for the stations; and (3) the classified non-maneuvering anomalies are detected by a new median robust method using the weak anomaly detection factor and the strong anomaly detection factor. The data from the Multi-GNSS Experiment (MGEX) in 2017 was used for experimental analysis. The experimental results and analysis showed that the start moment of orbital maneuvers and the period of non-maneuver anomalies can be determined more accurately in real-time. When orbital maneuvers and anomalies occur, the proposed method improved the data utilization for 91 and 95 min in 2017.

A Real-Time Robust Method to Detect BeiDou GEO/IGSO Orbital Maneuvers.

The frequent maneuvering of BeiDou Geostationary Orbit (GEO) and Inclined Geosynchronous Orbit (IGSO) satellites affects the availability of real-time orbit, and decreases the accuracy and performance of positioning, navigation and time (PNT) services. BeiDou satellite maneuver information cannot be obtained by common users. BeiDou broadcast ephemeris is the only indicator of the health status of satellites, which are broadcast on an hourly basis, easily leading to ineffective observations. Sometimes, identification errors of satellite abnormity also appear in the broadcast ephemeris. This study presents a real-time robust detection method for a satellite orbital maneuver with high frequency and high reliability. By using the broadcast ephemeris and pseudo-range observations, the time discrimination factor and the satellite identification factor were defined and used for the real-time detection of start time and the pseudo-random noise code (PRN) of satellites was used for orbital maneuvers. Data from a Multi-GNSS Experiment (MGEX) was collected and analyzed. The results show that the start time and the PRN of the satellite orbital maneuver could be detected accurately in real time. In addition, abnormal start times and satellite abnormities caused by non-maneuver factors also could be detected using the proposed method. The new method not only improves the utilization of observations for users with the data effective for about 92 min, but also promotes the reliability of real-time PNT services.