Design and test of rack-integrated cavity combiner for China initiative accelerator driven system project.

The next-generation 650 MHz solid state power amplifier designed by the Institute of Modern Physics will utilize 24 modules with an output power of 60 kW. The outputs of each of the 12 modules will be combined using a 12-in-1 rectangular cavity combiner integrated into the rack. This cavity combiner, requiring only a single stage to combine power, is characterized by a minimal power loss and a high combining efficiency. The input couplers of the combiner are adjustable to change the number of combination channels. In the event of one amplifier module failure, the corresponding port can be adjusted to decouple, transforming the combiner to an (N-1)-channel combiner with a combining efficiency decay of 0.2%. The prototype of the combiner has been fabricated and tested with a small signal. The combining efficiency is 98.5%. In this paper, we will validate the feasibility of the combiner from the design, simulation, and experiment.

Arbuscular mycorrhizal fungi improve selenium uptake by modulating root transcriptome of rice (Oryza sativa L.).

Although selenium (Se) is an essential trace element in humans, the intake of Se from food is still generally inadequate throughout the world. Inoculation with arbuscular mycorrhizal fungi (AMF) improves the uptake of Se in rice (Oryza sativa L.). However, the mechanism by which AMF improves the uptake of Se in rice at the transcriptome level is unknown. Only a few studies have evaluated the effects of uptake of other elements in rice under the combined effects of Se and AMF. In this study, Se combined with the AMF Funneliformis mosseae (Fm) increased the biomass and Se concentration of rice plants, altered the pattern of ionomics of the rice roots and shoots, and reduced the antagonistic uptake of Se with nickel, molybdenum, phosphorus, and copper compared with the treatment of Se alone, indicating that Fm can enhance the effect of fertilizers rich in Se. Furthermore, a weighted gene co-expression network analysis (WGCNA) showed that the hub genes in modules significantly associated with the genes that contained Se and were related to protein phosphorylation, protein serine/threonine kinase activity, membrane translocation, and metal ion binding, suggesting that the uptake of Se by the rice roots may be associated with these genes when Fm and Se act in concert. This study provides a reference for the further exploration of genes related to Se uptake in rice under Fm treatment.

Relative bioavailability of selenium in rice using a rat model and its application to human health risk assessment.

Deficiencies of selenium (Se), a necessary microelement for humans, can be remedied by appropriately supplying Se-enriched rice. However, overconsumption of Se-enriched rice poses a potential risk. To accurately assess Se human health risks associated with Se-enriched rice consumption, we developed a rat in vivo model to systematically explore the relative bioavailability of Se (Se-RBA) from Se-enriched rice from a wide geographic range. Se concentrations were in the range of 0.06 ± 0.05 to 0.15 ± 0.15 mg kg-1, averaging 0.12 ± 0.11 mg kg-1, in 196 rice samples from 21 Chinese provinces, and selenomethionine (SeMet) was the dominant Se fraction (58.0-96.5%). The Se-RBA of Se-enriched rice calculated from urine ranged from 34.86% to 102.29%, averaging 62.27% (n = 12), and was positively correlated with the proportion of SeMet in rice (p < 0.05, R2 = 0.51). Furthermore, the Se intake calculated based on the Se-RBA indicated that the Se intake of consumers of Se-enriched rice was far less than the tolerable upper intake level. Thus, the limits established by law assume overestimates of the actual nutritional value of the Se content in Se-enriched rice, and it is important to consider Se bioavailability. The current study offers suggestions for future research and provides methods to reduce the uncertainty in estimating the health risks associated with Se intake from rice.

Bi nanoparticles confined in N,S co-doped carbon nanoribbons with excellent rate performance for sodium-ion batteries.

Bismuth (Bi) has emerged as a promising candidate for sodium-ion battery anodes because of its unique layered crystal structure, superior volumetric capacity, and high theoretical gravimetric capacity. However, the large volume expansion and severe aggregation of Bi during the alloying/dealloying reactions are extremely detrimental to cycling stability, which seriously hinders its practical application. To overcome these issues, we propose an effective synthesis of composite materials, encapsulating Bi nanoparticles in N,S co-doped carbon nanoribbons and composites with carbon nanotubes (N,S-C@Bi/CNT), using Bi2S3 nanobelts as templates. The uniform distribution of Bi nanoparticles and the structure of carbon nanoribbons can reduce the diffusion path of ions/electrons, efficiently buffer the large volume change and prevent Bi from aggregating during cycles. As expected, the N,S-C@Bi/CNT electrode shows superior sodium storage performance in half cells, including a high specific capacity (345.3 mA h g-1 at 1.0 A g-1), long cycling stability (1000 cycles), and superior rate capability (336.0 mA h g-1 at 10.0 A g-1).

Effect of Foliar Spraying of Gibberellins and Brassinolide on Cadmium Accumulation in Rice.

Cadmium (Cd) is one of the heavy metals that contaminate rice cultivation, and reducing Cd contamination in rice through agronomic measures is a hot research topic. In this study, foliar sprays of gibberellins (GA) and brassinolide (BR) were applied to rice under Cd stress in hydroponic and pot experiments. After foliar spraying of GR and BR, the biomass of rice plants grown in either hydroponics or soil culture was significantly higher or even exceeded that in the absence of Cd stress. In addition, photosynthetic parameters (maximum fluorescence values), root length and root surface area, and CAT, SOD and POD activities were significantly improved. The MDA content decreased in the shoots, suggesting that the application of GR and BA may have enhanced photosynthesis and antioxidant function to alleviate Cd stress. Furthermore, the BR and GA treatments decreased the Cd content of rice roots, shoots and grains as well as the Cd transfer coefficient. Cd chemical morphology analysis of rice roots and shoots showed that the proportion of soluble Cd (Ethanol-Cd and Water-Cd) decreased, whereas the proportion of NaCl-Cd increased. Analysis of the subcellular distribution of Cd in rice roots and above ground showed that the proportion of Cd in the cell wall increased after foliar spraying of GA and BR. The results indicate that after foliar application of GA and BR, more of the Cd in rice was transformed into immobile forms and was fixed in the cell wall, thus reducing the amount in the seeds. In summary, foliar sprays of GA and BR can reduce the toxic effects of Cd on rice plants and reduce the Cd content in rice grains, with GA being more effective.

ExtHT: A hybrid tracing method for cyber-attacks in power industrial control systems.

Tracing the sources of cyber-attacks in Power Industrial Control Systems (PICS) can help the defense systems to block the attacks, and support the decision of the grid control policies. However, there has been no work on the cyber-attack source traceback for PICS, and the methods for the Internet are not suitable for PICS in terms of fineness, real-time performance, and supporting communication protocols. Therefore, a method for tracing cyber-attacks in PICS is proposed. First, the communication network architecture of PICS and the cyber security threats to PICS are analyzed. Then, an extended hybrid tracing method (ExtHT) based on packet marking and packet logging is proposed. This method involves all the devices working at the data link layer and upper layers to achieve more fine-grained attack tracing. At the same time, taking the costs of attack tracing into consideration, a coarse-grained tracing mode is presented to improve the tracing speed. In addition, a log database optimization scheme is provided to reduce storage costs. To facilitate the application of this method in practice, a cyber-attack source tracing system and its deployment architecture are designed for PICS. Further, the applicability and limitations of ExtHT are analyzed, theory ratiocinations are given to justify our ExtHT, and the performance of our ExtHT is compared with that of existing mainstream methods. Finally, two cyber-attack scenarios against PICS are constructed and the feasibility of ExtHT is verified on them.

Dexmedetomidine Inhibits Inflammation to Alleviate Early Neuronal Injury via TLR4/NF-κB Pathway in Rats with Traumatic Brain Injury.

Purpose - This study aims to explore the potential mechanism of dexmedetomidine in terms of inhibiting inflammation to alleviate early neuronal injury via TLR4/NF-κB pathway in rats with traumatic brain injury. Methods - The model of brain injury was established in rats. After the model was established, the rats were randomly divided into five groups: Sham, Sham + DEX, TBI, TBI + vehicle, and TBI + DEX. Each group included 10 rats. The water content in the brain tissue was measured. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assays were performed on histopathological tissue sections to evaluate neuronal apoptosis. Enzyme-linked immunosorbent assay and PCR were applied to detect the levels of the inflammatory factors, TNF-α, IL-1ß, IL-6, and NF-κB. Results - TBI-challenged rats exhibited significant neuronal apoptosis, which was characterized via the wet-to-dry weight ratio, neurobehavioral functions, TUNEL assay results, and the levels of cleaved caspase-3, Bax upregulation, and Bcl-2, which were attenuated by DEX. Western blot, immunohistochemistry, and PCR results revealed that DEX promoted TLR4 expression and upregulated expression of the TLR4 downstream factors, HO-1 and NQO-1. Furthermore, DEX treatment markedly prevented the downregulation of inflammatory response factors, TNF-α, IL-1ß and NF-κB, and IL-6. Conclusion - Dexmedetomidine is able to inhibit inflammation and attenuate early neuronal injury in rats with acute brain injury, which may act on TLR4/NF-κB pathway.

Stomatal conductance bears no correlation with transpiration rate in wheat during their diurnal variation under high air humidity.

A good understanding of the response of photosynthesis rate (P N) and transpiration rate (Tr) to stomatal alteration during the diurnal variations is important to cumulative photosynthetic production and water loss of crops. Six wheat genotypes were studied for 2 years with pot cultivation in rain-shelter. Among different genotypes, stomatal conductance (g s) was significantly correlated with both P N and Tr. But for each genotype, though g s was significantly correlated with P N regardless of relative air humidity (RH) status and it was also significantly correlated with Tr under lower RH (LRH, 15.4%) and moderate RH (MRH, 28.3%), it was not correlated with Tr under higher RH (HRH, 36.7%) during the diurnal changes. The conditional correlation between g s and Tr of wheat evoked new thinking on the relationships among g s, P N and Tr. Path analysis was further carried out to clarify the correlations of g s with the four atmospheric factors, that of Tr with g s and the four factors and the direct and indirect effects of the factors, during their diurnal dynamic variation. The effects of these factors on g s or Tr were related to RH. All the four factors had a much higher correlation with g s under HRH than that under LRH and MRH. Air temperature (T) had a rather higher direct effect than RH and photosynthetically active radiation (PAR). Also, the other factors had a much higher indirect effect on g s through vapor pressure deficit (VPD) and T. Transpiration rate was highly correlated with g s under LRH and MRH, with g s having a higher direct effect on it. In comparison, Tr was not correlated with g s under HRH but highly correlated with the atmospheric factors, with T, RH, and PAR having a higher indirect effect through VPD.

Dynamic changes in membrane lipid composition of leaves of winter wheat seedlings in response to PEG-induced water stress.

BACKGROUND: Membrane lipid composition associates closely with membrane stability and fluidity under water stress. In this study, lipidomic analyses based on electrospray ionization mass spectrometry (ESI-MS/MS) were carried out to explore dynamic changes of membrane lipids in term of molecular species caused by PEG (Polyethylene glycol-6000)-induced water stress in wheat seedlings. RESULTS: Among the main phospholipids, phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylglycerol (PG) are primary degradation targets, and PC was degraded in the largest degree. Membrane ion leakage dramatically increased later than the significant reduction of these phospholipids, indicating that the loss of membrane integrity lagged behind severe phospholipid degradation. Monogalactosyldiacylglycerol (MGDG) increased firstly and decreased later, while digalactosyldiacylglycerol (DGDG) ratcheted up with stress. DGDG/MGDG increased after stress for 3 days, and unsaturation of DGDG was promoted with stress. Variation trends of galactolipids differed among molecular species. The time when MGDG (34:3), DGDG (34:3) began to decline approached to the time when non-stomatal limitation impaired photosynthesis. While the two predominant molecular species MGDG (36:6) and DGDG (36:6) began to decline later. So we speculated that MGDG (34:3), DGDG (34:3) might be key components in photosynthesis apparatus and participate in photosynthesis directly. While the two predominant molecular species, MGDG (36:6) and DGDG (36:6) might locate in thylakoid lipid bilayer matrix and play roles in stabilizing the membrane. The research provides new insights into the dynamic response of lipid metabolism to PEG-induced water stress. CONCLUSION: In wheat plants under water stress, the major molecular species of PC, PE and PG were degraded, MGDG and DGDG molecular species had differing degradation time courses.

LncRNA MALAT1 is involved in sevoflurane-induced neurotoxicity in developing rats.

OBJECTIVE: The purpose of this study is to uncover the effects of long chain noncoding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) on sevoflurane-induced neurotoxicity in developing rats. METHODS: Sevoflurane neurotoxicity model was established by sevoflurane treatment in 7-day-old Sprague-Dawley rats. The rats were treated with Sevo or MALAT1 small interfering RNA to detect the MALAT1 expression, pathological change, ultrastructure, neuronal apoptosis, expression of apoptosis-related proteins, expression of neurotrophic factors BDNF and NGF, spatial learning and memory function change, as well as neuron cell density of hippocampal tissues. RESULTS: MALAT1 was highly expressed in hippocampus tissues of rats. Downregulation of MALAT1 alleviated the pathological change, improved the ultrastructure, inhibited apoptosis of neuronal cells, declined caspase 3 and Bax while elevated Bcl-2, BDNF and NGF, improved capability of spatial learning and memory, and increased density of hippocampal neurons in hippocampal tissues of sevoflurane-induced rats. CONCLUSION: Suppression of MALAT1 can reduce the apoptosis of hippocampal neurons induced by sevoflurane anesthesia, improve the capability of spatial learning, and memory function and alleviate the loss of hippocampal nerve cells in developing rats. To a certain extent, it plays the role of protecting brain nerve cells.

Downregulation of microRNA-155 stimulates sevoflurane-mediated cardioprotection against myocardial ischemia/reperfusion injury by binding to SIRT1 in mice.

OBJECTIVE: The inhaled sevoflurane has been demonstrated to protect against myocardial ischemia/reperfusion (I/R) injury. However, the relative mechanisms of sevoflurane-mediated cardioprotection remain largely unknown. This study intends to explore the effect of miR-155 on the sevoflurane-mediated cardioprotection by regulating Sirtuin 1 (SIRT1) in mouse models of myocardial I/R. METHODS: Left anterior descending coronary artery ligation was used to induce models of myocardial I/R in mice. The I/R mice were treated with sevoflurane, sevoflurane + mimics negative control (NC) or sevoflurane + miR-155 mimics. The expression of microRNA-155 (miR-155) and SIRT1 was examined by quantitative real-time polymerase chain reaction and Western blot assay. Then cardiac functions and hemodynamic alterations were evaluated. Evans blue-2,3,5-triphenyltetrazolium chloride and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay staining methods were adopted to evaluate infarct size and cardiomyocyte apoptosis, respectively. RESULTS: In the I/R mice, miR-155 was expressed at a high level and SIRT1 at a low level. SIRT1 was confirmed to be a target gene of miR-155. The treatment of sevoflurane could reduce miR-155 expression and increased SIRT1 expression in the myocardial tissues, under which conditions, cardiac functions were promoted, accompanied by reduced infarct size and inhibited cardiomyocyte apoptosis. In response to miR-155 upregulation, the sevoflurane-treated I/R mice showed reduced cardiac functions, and increased infarct size and cardiomyocyte apoptosis. CONCLUSION: The findings obtained in this study provide evidence suggesting that miR-155 targets and negatively regulates SIRT1 expression, a mechanism by which the protection of sevoflurane is inhibited against myocardial I/R in mice.

iTRAQ-Based Quantitative Analysis of Responsive Proteins Under PEG-Induced Drought Stress in Wheat Leaves.

Drought is an important abiotic stress that seriously restricts crop productivity. An understanding of drought tolerance mechanisms offers guidance for cultivar improvement. In order to understand how a well-known wheat genotype Jinmai 47 responds to drought, we adopted the iTRAQ and LC/MS approaches and conducted proteomics analysis of leaves after exposure to 20% of polyethylene glycol-6000 (PEG)-induced stress for 4 days. The study identified 176 differentially expressed proteins (DEPs), with 65 (36.5%) of them being up-regulated, and 111 (63.5%) down-regulated. DEPs, located in cellular membranes and cytosol mainly, were involved in stress and redox regulation (51), carbohydrate and energy metabolism (36), amino acid metabolism (24), and biosynthesis of other secondary metabolites (20) primarily. Under drought stress, TCA cycle related proteins were up-regulated. Antioxidant system, signaling system, and nucleic acid metabolism etc. were relatively weakened. In comparison, the metabolism pathways that function in plasma dehydration protection and protein structure protection were strongly enhanced, as indicated by the improved biosynthesis of 2 osmolytes, sucrose and Proline, and strongly up-regulated protective proteins, LEA proteins and chaperones. SUS4, P5CSs, OAT, Rab protein, and Lea14-A were considered to be important candidate proteins, which deserve to be further investigated.

Comparisons of WUE in twelve genotypes of winter wheat and the relationship between δ 13C and WUE.

Twelve winter wheat (Triticum aestivum) genotypes were examined for differences in grain yield, water use efficiency (WUE), and stable carbon isotope composition (δ13C) in flag leaves. The plants were subjected to rain-fed treatment and supplemental irrigation at the jointing and anthesis stages, during the 2015-2016 and 2016-2017 winter wheat growing seasons. The relationships between δ13C with grain yield and WUE were analyzed under two different water environments. The results indicated that there were significant differences in δ13C, grain yield, and WUE among wheat genotypes both under rain-fed and supplemental irrigation conditions. The δ13C values increased with grain-filling proceeding, the δ13C being lower under supplemental irrigation treatment than that under rain-fed treatment. The relationships between the average of δ13C with grain yield and WUE were significantly positive during three measurement periods (R2 = 0.5785 - 0.8258), whether under rain-fed or irrigation environments. This suggests that δ13C might be associated with the grain yield and WUE in winter wheat under rain-fed and supplemental irrigation conditions in the climate region of the northwest Huang-Huai-Hai Plain of China.

[Effect of electroacupuncture of "Mingmen" (GV 4) on bone morphogenetic protein-2 expression in osteoporosis rats].

OBJECTIVE: To observe the effect of electroacupuncture (EA) stimulation of "Mingmen" (GV 4) on the bone morphogenetic protein-2 (BMP-2) content and biomechanics in osteoporosis rats so as to explore its mechanism underlying improvement of osteoporosis. METHODS: Fifty female SD rats were randomized into sham operation (sham), model, EA-GV 4, EA-non-acupoint (non-acupoint) and estrogen (medication) groups, with 10 rats in each group. Postmenopausal osteoporosis model was established by removing the rats' bilateral ovaries under anesthesia. EA (2 Hz/15 Hz, 1.0 mA) was applied to "Mingmen" (GV 4) or non-acupoint for 20 min, once daily for 30 times, with one day's interval between every two 10 times. Rats of the medication group were lavaged with Pentanoic Acid Estradiol (25 microg/mL, 2 mL/500 g), once every day (the dosage of estradiol was adjusted according to their body weight) continuously for 1 month. Rats of the model and sham groups experienced the fixing and fastening procedures as the other rats in the EA and medication groups. After intervention, the BMP-2 expression level of the femoral bone tissue, and bone biomechanical values were determined by immunohistochemistry and three-point bending tests, respectively. RESULTS: (1) In comparison with the sham operation group, the femoral biochemical maximum load and fracture load values were significantly decreased in the model group (P < 0.05). While compared with the model group, the biochemical maximum load and fracture load values were obviously increased in the EA-GV 4 and medication groups (P < 0.05), but not in the non-acupoint group (P > 0.05). (2) Compared with the sham group, the femoral BMP-2 expression of model group was significantly decreased (P < 0.05), compared with the model group, the expression of BMP-2 of GV 4 and medication groups significantly increased (P < 0.05). CONCLUSION: EA-GV 4 intervention can improve bone biomechanical changes in osteoporosis rats.

[Our considerations about non-acupoint selection for experimental studies in rats].

Researches on the specificity of acupoints need a contrast of non-acupoint. However, there have been no uniform standards for locating the non-acupoint in both human body and animals. In the present paper, the authors make a review about the methods for locating the non-acupoint in rats during acupuncture experiments in recent decade. Till now, four methods were frequently used, i.e., 1) selecting a spot beside the known acupoint at the same level, 2) selecting the mid point between two meridian running courses, 3) choosing a spot at the tail, and 4) choosing a spot below the costal region. The authors hold that of the 4 methods, the last one, i.e., taking the spot below the costal region as the non-acupoint is probably the most reasonable approach, if it is far from the observed acupoint, and on the same or the adjacent spinal nerve segment.

[Discussion on method for locating acupoint "Mingmen" (GV 4) in adult rats].

"Mingmen" (GV 4) is one of the most frequently used acupoints in acupuncture clinic. In recent years, more and more experimental researches have been focusing on GV 4 or acupoint recipe containing GV 4 in rats. Accurate location of GV 4 is probably not only related to fully display its therapeutic effect, but also to help study its underlying mechanisms. However, there has been no unified standard about the accurate location of GV 4 in the adult rat at present. In the present paper, the authors review related literature about GV 4 location in experimental studies in recent 10 years, and put forward a practical method for locating GV 4 in the rat by combining their own experience. That is, GV 4 is taken according to the relative relationship of ilium and spinous process of the lumbar vertebra. In addition, the authors also recommend some matters needing attention in locating GV 4 in rats.

Inhibition of HBV infection by bovine lactoferrin and iron-, zinc-saturated lactoferrin.

In this study, we investigated the effect of bovine lactoferrin (BLf), lactoferrin hydrolysate, or iron-, zinc-saturated lactoferrin on hepatitis B virus (HBV)-infected HepG2 cells. Fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to quantify HBV-DNA copies. BLf, iron- or zinc-saturated lactoferrin significantly inhibited the amplification of HBV-DNA in a dose-dependent manner in HBV-infected HepG2 cells. However, the inhibitive effect of lactoferrin hydrolysate on HBV-DNA copies was insignificant. These findings suggest that BLf inhibits the function of HBV by integrated structure. In conclusion, BLf, iron- or zinc-saturated BLf is one of the candidates for anti-HBV reagents in treatment of patients with hepatitis.

[Study of inhibition effect of zinc-, iron- and manganese-saturated bovine lactoferrin on hepatitis B virus DNA in vitro].

OBJECTIVE: To probe inhibition effect of zinc-saturated (Zn2+ -BLF), iron-saturated (Fe2+ -BLF) and manganese-saturated (Mn2+ -BLF) bovine lactoferrin on hepatitis B virus DNA (HBV-DNA) in vitro. METHODS: Nature HBV was used to infect HepG2 cell, HBV-DNA was measured by fluorescence polymerase chain reaction to detect the effect of Zn2+ -BLF, Fe2+ -BLF and Mn2+ -BLF, the MTT test was used to examine cytotoxic effect of Zn2+ -BLF, Fe2+ -BLF and Mn2+ -BLF. RESULTS: The maximum nontoxic dose (TD0) in HepG2 cell of Zn2+ -BLF, Fe2+ -BLF and Mn2+ -BLF were 1.5,3.0 and 1.5 g/L respective. After HepG2 cell were infected with HBV, every Zn2+ -BLF, Fe2+ -BLF and Mn2+ -BLF test group had effect on inhibiting HBV-DNA to some extent. Compared the inhibition effect of concentration of 1.5 g/ L test group in each term, the inhibition result of Zn2 -BLF was 5.746 +/- 0.114, the inhibition result of Fe2+ -BLF was 6. 446 +/- 0.103, and the inhibition result of Mn2+ -BLF was 5.999 +/- 0.725. CONCLUSION: After HepG2 cell was infected with HBV, HBV-DNA could be inhibited by Zn2+ -BLF, Fe2+ -BLF and Mn2+ -BLF. Zn2+ -BLF has higher inhibition effect than Mn2+ -BLF, and Fe2+ -BLF has the lowest inhibition effect, but the possible mechanism need to be lucubrated.

[Study of inhibition effect of bovine lactoferrin in vitro on hepatitis B surface antigen].

OBJECTIVE: To probe inhibition effect of bovine lactoferrin (BLF) in vitro on hepatitis B surface antigen (HBsAg). METHODS: Nature hepatitis B virus (HBV) was used to infect HepG2 cell, HBsAg in the culture supernatant was measured by ELISA, and MTT test was used to examine cytotoxic effect of BLF. RESULTS: The maximum nontoxic dose (TD0) in HepG2 cell of BLF was 3.0 g/L,50% of toxic dose (TD50) was 11.08 g/L. After HepG2 cell were infected with HBV, every BLF test group had effect on inhibiting HBsAg to some extent. At the highest concentration of 3.0 g/L BLF was most effective, exhibiting 58.34% of inhibition. Mixing BLF with HBV, then HepG2 cell were infected with them, every BLF all test concentrations were not shown to inhibit HBsAg. Adding BLF to HepG2 cell, then HepG2 cell were infected with HBV after washing BLF, test groups were shown to significantly inhibite HBsAg as concentration of over 0.5 g/L BLF, but at the concentration 0.1 g/L of BLF inhibitious of HBsAg were not observed. HBsAg wasn't inhibited by lactoferrin hydrolysate. CONCLUSION: BLF was shown to inhibit HBV infection into HepG2 cell by protecting corresponding cell sites rather than binding to HBV. After HepG2 cell was infected, HBsAg also could be inhibited by BLF and lactoferrin hydrolysate didn't have this function. It was seemed that BLF could have structure's work, but the possible mechanism need to be lucubrated.

[Sero-epidemiologic study on the relationship between Chlamydia pneumonia infection and hypertension in Chinese Mongolian population].

OBJECTIVE: To explore the relationship between Chlamydia pneumonia (CP) infection and hypertension as well as the interaction of traditional risk factors and CP infections on hypertension in Chinese Mongolian population. METHODS: 1430 inhabitants living in Tongliao city, Inner Mongolia were selected as research subjects, including 488 hypertensives and 942 normotensives. Enzyme-linked immunoassay technique was used to test CP IgG antibody in the blood serum and SPSS 13.0 Microsoft was used to analyze the data. RESULTS: The prevalence rates of hypertension were significantly different between CP IgG positive and negative groups, However, the trend was attenuated after adjusting age, sex, drinking, smoking, body mass index (BMI) and dyslipidemia. The CP infection rate was significant different in diastolic blood pressure groups, which was significantly higher in 80-84 mm Hg than that in the lowest DBP group. The interaction of chronic CP infection and other traditional risk factors were associated with hypertension in Chinese Mongolian ethnic groups. The ranking with significant sequence from high to low were BMI, dyslipidemia, alcohol intake, sex and age. CONCLUSION: CP infection was not related to hypertension in Chinese Mongolian ethnic groups but the interaction with other traditional risk factors would increase the risk of developing hypertension.