3D-Printing of Hierarchical Porous Copper-Based Metal-Organic-Framework Structures for Efficient Fixed-Bed Catalysts.

Metallic structures with hierarchical open pores that span several orders of magnitude are ideal candidates for various catalyst applications. However, porous metal materials prepared using alloy/dealloy methods still struggle to achieve continuous pore distribution across a broad size range. Herein, we report a printable copper (Cu)/iron (Fe) composite ink that produces a hierarchical porous Cu material with pores spanning over 4 orders of magnitude. The manufacturing process involves four steps: 3D-printing, annealing, dealloying, and reannealing. Because of the unique annealing process, the resulting hierarchical pore surface becomes coated with a layer of Cu-Fe alloy. This feature imparts remarkable catalytic ability and versatile functionality within fixed bed reactors for 4-nitrophenol (4-NP) reduction and Friedländer cyclization. Specifically, for 4-NP reduction, the porous Cu catalyst demonstrates an excellent reaction rate constant (kapp = 86.5 × 10-3 s-1) and a wide adaptability of the substrate (up to 1.26 mM), whilst for Friedländer cyclization, a conversion over 95% within a retention time of only 20 min can be achieved by metal-organic-framework-decorated porous Cu catalyst. The utilization of dual metallic particles as printable inks offers valuable insights for fabricating hierarchical porous metallic structures for applications, such as advanced fixed-bed catalysts.

Efficient Sequential Detection of Two Antibiotics Using a Fiber-Optic Surface Plasmon Resonance Sensor.

Antibiotic residues have become a worldwide public safety issue. It is vital to detect multiple antibiotics simultaneously using sensors. A new and efficient method is proposed for the combined detection of two antibiotics (enrofloxacin (Enro) and ciprofloxacin (Cip)) in milk using surface plasmon resonance (SPR) sensors. Based on the principle of immunosuppression, two antibiotic antigens (for Enro and Cip) were immobilized on an optical fiber surface with conjugates of bovine serum albumin using dopamine (DA) polymerization. Each single antigen was bound to its corresponding antibody to derive standard curves for Enro and Cip. The fiber-optic sensor's sensitivity was 2900 nm/RIU. Detection limits were calculated to be 1.20 ng/mL for Enro and 0.81 ng/mL for Cip. The actual system's recovery rate was obtained by testing Enro and Cip in milk samples; enrofloxacin's and ciprofloxacin's mean recoveries from the milk samples were 96.46-120.46% and 96.74-126.9%, respectively. In addition, several different regeneration solutions were tested to analyze the two target analytes' regeneration ability; NaOH and Gly-HCl solutions were found to have the best regeneration ability.

Galvanic Replacement Synthesis of VOx@EGaIn-PEG Core-Shell Nanohybrids for Peroxidase Mimics.

The development of high-performance biosensors is a key focus in the nanozyme field, but the current limitations in biocompatibility and recyclability hinder their broader applications. Herein, we address these challenges by constructing core-shell nanohybrids with biocompatible poly(ethylene glycol) (PEG) modification using a galvanic replacement reaction between orthovanadate ions and liquid metal (LM) (VOx@EGaIn-PEG). By leveraging the excellent charge transfer properties and the low band gap of the LM surface oxide, the VOx@EGaIn-PEG heterojunction can effectively convert hydrogen peroxide into hydroxyl radicals, demonstrating excellent peroxidase-like activity and stability (Km = 490 µM, vmax = 1.206 µM/s). The unique self-healing characteristics of LM further enable the recovery and regeneration of VOx@EGaIn-PEG nanozymes, thereby significantly reducing the cost of biological detection. Building upon this, we developed a nanozyme colorimetric sensor suitable for biological systems and integrated it with a smartphone to create an efficient quantitative detection platform. This platform allows for the convenient and sensitive detection of glucose in serum samples, exhibiting a good linear relationship in the range of 10-500 µM and a detection limit of 2.35 µM. The remarkable catalytic potential of LM, combined with its biocompatibility and regenerative properties, offers valuable insights for applications in catalysis and biomedical fields.

Shear-Induced Fabrication of Cellulose Nanofibril/Liquid Metal Nanocomposite Films for Flexible Electromagnetic Interference Shielding and Thermal Management.

To address electromagnetic interference (EMI) pollution in modern society, the development of ultrathin, high-performance, and highly stable EMI shielding materials is highly desired. Liquid metal (LM) based conductive materials have received enormous amounts of attention. However, the processing approach of LM/polymer composites represents great challenges due to the high surface tension and cohesive energy of LMs. In this study, we develop a universal one-step fabrication strategy to directly process composites containing LMs and cellulose nanofibrils (CNFs) and successfully fabricate the ultrathin, flexible, and stable EMI shielding films with an average specific EMI shielding efficiency (EMI SE) value of 429 dB/mm and small thickness of only 70 µm in the wide frequency range of 8.2-18 GHz. In addition, the resulting films also exhibit excellent mechanical performance and flexibility, which endow the film with the ability to withstand repeated folding, bending, and folding into complex shapes without producing cracks or fractures. Besides, the resulting films display excellent thermal conductivity with a λ of 4.90 W/(m K) and an α of 3.17 mm2/s. Thus, the presented approach shows great potential in fabricating advanced materials for EMI shielding applications.

Wild species rice OsCERK1DY-mediated arbuscular mycorrhiza symbiosis boosts yield and nutrient use efficiency in rice breeding.

Meeting the ever-increasing food demands of a growing global population while ensuring resource and environmental sustainability presents significant challenges for agriculture worldwide. Arbuscular mycorrhizal symbiosis (AMS) has emerged as a potential solution by increasing the surface area of a plant's root system and enhancing the absorption of phosphorus, nitrogen nutrients, and water. Consequently, there is a longstanding hypothesis that rice varieties exhibiting more efficient AMS could yield higher outputs at reduced input costs, paving the way for the development of Green Super Rice (GSR). Our prior research study identified a variant, OsCERK1DY, derived from Dongxiang wild-type rice, which notably enhanced AMS efficiency in the rice cultivar "ZZ35." This variant represents a promising gene for enhancing yield and nutrient use efficiency in rice breeding. In this study, we conducted a comparative analysis of biomass, crop growth characteristics, yield attributes, and nutrient absorption at varying soil nitrogen levels in the rice cultivar "ZZ35" and its chromosome single-segment substitution line, "GJDN1." In the field, GJDN1 exhibited a higher AM colonization level in its roots compared with ZZ35. Notably, GJDN1 displayed significantly higher effective panicle numbers and seed-setting rates than ZZ35. Moreover, the yield of GJDN1 with 75% nitrogen was 14.27% greater than the maximum yield achieved using ZZ35. At equivalent nitrogen levels, GJDN1 consistently outperformed ZZ35 in chlorophyll (Chl) content, dry matter accumulation, major nutrient element accumulation, N agronomic efficiency (NAE), N recovery efficiency (NRE), and N partial factor productivity (NPFP). The performance of OsCERK1DY overexpression lines corroborated these findings. These results support a model wherein the heightened level of AMS mediated by OsCERK1DY contributes to increased nitrogen, phosphorus, and potassium accumulation. This enhancement in nutrient utilization promotes higher fertilizer efficiency, dry matter accumulation, and ultimately, rice yield. Consequently, the OsCERK1DY gene emerges as a robust candidate for improving yield, reducing fertilizer usage, and facilitating a transition towards greener, lower-carbon agriculture. Supplementary Information: The online version contains supplementary material available at 10.1007/s11032-024-01459-8.

Autoimmune Glial Fibrillary Acidic Protein Astrocytopathy Is Associated with HLA-A*3303 and HLA-DPB1*0501.

We determined the genetic association between specific human leucocyte antigen (HLA) loci and autoimmune glial fibrillary acidic protein (GFAP) astrocytopathy. Our results showed that autoimmune GFAP astrocytopathy was associated with HLA-A*3303 (odds ratio [OR] = 2.02, 95% confidence interval [CI] = 1.32-3.06, p = 0.00072, padj. = 0.046) and HLA-DBP1*0501 (OR = 0.51, 95% CI = 0.36-0.71, p = 0.000048, padj. = 0.0062). Moreover, HLA-A*3303 carriers with the disease had a longer hospital stay (p = 0.0005) than non-carriers. This study for the first time provides evidence for a role of genetic factor in the development of autoimmune GFAP astrocytopathy. ANN NEUROL 2024;95:901-906.

Efficient composite chlorinated ethenes removal using gallic acid to enhance Fe/Ni nanoparticles activated persulfate.

As the representative volatile chlorinated hydrocarbons detected in wastewater, the removal of composite chlorinated ethenes is a major challenge in wastewater treatment. In the present study, an efficient removal system for composite chlorinated ethenes was reported, in which gallic acid was used to enhance the activation of persulfate by Fe/Ni nanoparticles. The influences of gallic acid-Fe/Ni and persulfate concentrations, initial pH value, reaction temperature, inorganic anions, and natural organic matters were evaluated in the composite chlorinated ethenes removal. Our results showed that the gallic acid-Fe/Ni-persulfate system with 9.0 mM of gallic acid-Fe/Ni and 30.0 mM of persulfate yielded about 100% trichloroethylene removal and 97.3%-98.6% perchloroethylene removal in the pH range of 3.0-12.0. Electron paramagnetic resonance analysis and radical quenching experiments indicated that SO4•- and •OH were the predominant radical species under acidic and alkaline conditions. Ultraviolet visible spectroscopy and inductively coupled plasma optical emission spectrometer tests revealed the Fe-gallic acid chelation could regulate the concentration of iron ions and improve the reactivity of gallic acid-Fe/Ni. These results demonstrated that the gallic acid-Fe/Ni-persulfate system was a promising strategy for treating composite chlorinated ethenes-containing wastewater.

Protocol for 3D and 4D printing of highly conductive metallic composite using liquid metal gels.

3D or 4D printing of metal structures requires extreme conditions or a multistage process. Here, we present a protocol for the preparation of highly conductive metallic composites using liquid metal gels at ambient conditions. We describe the steps to prepare ternary gels composed of copper particles, liquid metal, and water. We then detail procedures for 3D or 4D printing gels into highly conductive structures after adding a small amount of rheological modifier (methyl cellulose) using direct ink writing techniques. For complete details on the use and execution of this protocol, please refer to Xing et al. (2023).1.

Tannic acid promotes the activation of persulfate with Fe(ii) for highly efficient trichloroethylene removal.

Trichloroethylene (TCE) is an Environmental Protection Agency (EPA) priority pollutant that is difficult to be removed by some remediation methods. For instance, TCE removal using persulfate (PS) activated by ferrous iron (Fe(ii)) has been tested but is limited by the unstable Fe(ii) concentration and the initial pH of contaminated water samples. Here we reported a new TCE removal system, in which tannic acid (TA) promoted the activation of PS with Fe(ii) (TA-Fe(ii)-PS system). The effect of initial pH, temperature, and concentrations of PS, Fe(ii), TA, inorganic anions and humic acid on TCE removal was investigated. We found that the TA-Fe(ii)-PS system with 80 mg L-1 of TA, 1.5 mM of Fe(ii) and 15 mM of PS yielded about 96.2-99.1% TCE removal in the pH range of 1.5-11.0. Radical quenching experiments were performed to identify active species. Results showed that SO4˙- and ˙OH were primarily responsible for TCE removal in the TA-Fe(ii)-PS system. In the presence of TA, the Fe-TA chelation and the reduction of TA could regulate Fe(ii) concentration and activate persulfate for continuously releasing reactive species under alkaline conditions. Based on the excellent removal performance for TCE, the TA-Fe(ii)-PS system becomes a promising candidate for controlling TCE in groundwater.

A rapid removal of Phaeocystis globosa from seawater by peroxymonosulfate enhanced cellulose nanocrystals coagulation.

Cellulose nanocrystals (CNC) are recognized as promising bio-based flocculants for controlling harmful algal blooms (HABs). Due to the charge shielding effect in seawater and the strong mobility of algae cells, CNC can't effectively remove Phaeocystis globosa from seawater. To solve this problem, peroxymonosulfate (PMS) was used to enhance the coagulation of CNC for rapidly removal of P. globosa. The results showed that 91.7% of Chl-a, 95.2% of OD680, and 97.2% of turbidity of P. globosa were reduced within 3 h with the use of 200 mg L-1 of CNC and 20 mg L-1 of PMS. The removal of P. globosa was consisted of inactivation and flocculation. Notably, electron paramagnetic resonance (EPR) spectrums and quenching experiments revealed that the inactivation of P. globosa was dominated by PMS oxidation and 1O2. Subsequently, CNC entrained inactivated algal cells to settle to the bottom to achieve efficient removal of P. globosa. The content of total organic carbon (TOC) and chemical oxygen demand (COD) decreased significantly, indicating that a low emission risk of algal cell effluent was produced in the CNC-PMS system. In view of the excellent performance on P. globosa removal, we believe that the CNC-PMS system has great potential for HABs treatments.

Efficient removal of chloroform from groundwater using activated percarbonate by cellulose nanofiber-supported Fe/Cu nanocomposites.

Chloroform (CF) is a recalcitrant halogenated methane (HM) that has received widespread attention due to its frequent detection in groundwater and its potential carcinogenic risk. In this study, TEMPO-oxidized cellulose nanofiber-supported iron/copper bimetallic nanoparticles (TOCNF-Fe/Cu), a novel composite catalyst, was synthesized to activate sodium percarbonate (SPC) for the removal of CF from groundwater. The results showed that over 96.3% of CF could be removed in a neutral reaction medium (pH 6.5-9) within 180 min using 0.66 g L-1 of TOCNF (0.32)-Fe/Cu (1) and 1 mM of SPC, which outperforms typical advanced oxidation processes. The reaction mechanism of the TOCNF-Fe/Cu-SPC system for the CF removal was elucidated. As demonstrated through electron paramagnetic resonance and quenching experiments, the TOCNF-Fe/Cu-SPC system was found to include •OH and O2•-, where the latter played a dominant role in the CF removal. DFT calculations indicated that TOCNF improved the electron transport capability of Fe/Cu and reduced the transition state energy. The Fe species on the surface of TOCNF-Fe/Cu were identified as the primary active sites for SPC activation, whereas the Cu species were beneficial to the regeneration of the Fe species. Additionally, TOCNF-Fe/Cu was found to have good recyclability and stability. The feasibility of the TOCNF-Fe/Cu-SPC system was further confirmed by applying it for the efficient removal of composite HMs from actually contaminated groundwater. Overall, the TOCNF-Fe/Cu-SPC system is an attractive candidate for the treatment of HM-contaminated groundwater.

MG1 interacts with a protease inhibitor and confers resistance to rice root-knot nematode.

The rice root-knot nematode (Meloidogyne graminicola) is one of the most destructive pests threatening rice (Oryza sativa L.) production in Asia; however, no rice resistance genes have been cloned. Here, we demonstrate that M. GRAMINICOLA-RESISTANCE GENE 1 (MG1), an R gene highly expressed at the site of nematode invasion, determines resistance against the nematode in several rice varieties. Introgressing MG1 into susceptible varieties increases resistance comparable to resistant varieties, for which the leucine-rich repeat domain is critical for recognizing root-knot nematode invasion. We also report transcriptome and cytological changes that are correlated with a rapid and robust response during the incompatible interaction that occurs in resistant rice upon nematode invasion. Furthermore, we identified a putative protease inhibitor that directly interacts with MG1 during MG1-mediated resistance. Our findings provide insight into the molecular basis of nematode resistance as well as valuable resources for developing rice varieties with improved nematode resistance.

Genome editing of a rice CDP-DAG synthase confers multipathogen resistance.

The discovery and application of genome editing introduced a new era of plant breeding by giving researchers efficient tools for the precise engineering of crop genomes1. Here we demonstrate the power of genome editing for engineering broad-spectrum disease resistance in rice (Oryza sativa). We first isolated a lesion mimic mutant (LMM) from a mutagenized rice population. We then demonstrated that a 29-base-pair deletion in a gene we named RESISTANCE TO BLAST1 (RBL1) caused broad-spectrum disease resistance and showed that this mutation caused an approximately 20-fold reduction in yield. RBL1 encodes a cytidine diphosphate diacylglycerol synthase that is required for phospholipid biosynthesis2. Mutation of RBL1 results in reduced levels of phosphatidylinositol and its derivative phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). In rice, PtdIns(4,5)P2 is enriched in cellular structures that are specifically associated with effector secretion and fungal infection, suggesting that it has a role as a disease-susceptibility factor3. By using targeted genome editing, we obtained an allele of RBL1, named RBL1Δ12, which confers broad-spectrum disease resistance but does not decrease yield in a model rice variety, as assessed in small-scale field trials. Our study has demonstrated the benefits of editing an LMM gene, a strategy relevant to diverse LMM genes and crops.

Nanocellulose-based hydrogels for drug delivery.

Hydrogels, as a class of three-dimensional (3D) polymer networks, are important candidates for drug delivery owing to their high porosity and hydrophilicity. Generally, clinical applications put forward various requirements for drug delivery systems (DDSs), such as low toxic side effects, high biocompatibility, targeting, controllable release, and high drug loading. In recent years, nanocellulose, including cellulose nanocrystals (CNCs) and cellulose nanofibrils (CNFs), has emerged as a promising material for hydrogel-based DDSs. This is due to its high surface area, abundant surface hydroxyl groups that can be easily chemically modified for multifunctionalization, natural origin leading to high biocompatibility and degradability, etc. This review provides a comprehensive overview of the preparation methods for hydrogels based on CNCs/CNFs for use in drug delivery systems, including physical crosslinking and chemical crosslinking. Additionally, various carrier forms such as hydrogel particles, hydrogel films, injectable hydrogels, and sprayable hydrogels are discussed. Key drug delivery parameters including loading and release efficiency as well as responses to different stimuli are also examined in detail. Finally, in view of the subdivision of drug delivery, the opportunities and challenges of nano cellulose based hydrogels were proposed from the perspective of application, and the potential research directions were pointed out.

Spray-Drying Hydrophobic Cellulose Nanocrystal Coatings with Degradable Biocide Release for Marine Antifouling.

With increasing awareness about the ecological environment, increased attention has been paid to the application of eco-friendly materials in the field of marine antifouling. In this work, a novel coating having good mechanical strength and static marine antifouling characteristics was fabricated using cellulose nanocrystals (CNCs) as the skeleton material, with in situ growth of SiO2 as the basic superhydrophobic material and introducing hexadecyl trimethyl ammonium bromide (CTAB) and 4-bromo2-(4-chlorophenyl)-5-(trifluoromethyl)-1H-pyrrole-3-carbonitrile (Econea) into the coating. Due to the high strength and rod structure of CNCs, the coating maintained super-hydrophobicity after 50 cycles of abrasion tests. Moreover, the addition of CTAB during the synthesis of SiO2 led to the hydrolysis and polycondensation of tetraethyl orthosilicate at the micellar interface. Econea was fully mixed with SiO2 nanoparticles, thus slowing down the rate of release of Econea. Meanwhile, the adhesion between the coating and the substrate reached 1.9 MPa, which can meet the application requirements for marine environments. The bioassay using bacteria (Escherichia coli) and diatoms (Nitzschia closterium) showed that the rate of inhibition of the coating on bacteria and diatoms could reach 99 and 90%, respectively, after immersion in artificial seawater for 28 days. This research provides a facile and promising fabricating solution of an eco-friendly CNC-based coating having strong antifouling characteristics suitable for marine environments.

Anisotropic cellulose nanocrystalline sponge sheets with ultrahigh water fluxes and oil/water selectivity.

Oily sewage caused by oil spill accidents has become a severe problem in the last decades. Hence, two-dimensional sheet-like filter materials for oil/water separation have received widespread attention. Porous sponge materials were developed using cellulose nanocrystals (CNCs) as raw materials. They are environmentally friendly and easy to prepare, with high flux and separation efficiency. The 1,2,3,4-butane tetracarboxylic acid cross-linked anisotropic cellulose nanocrystalline sponge sheet (B-CNC) exhibited ultrahigh water fluxes driven by gravity alone, depending on the aligned structure of channels and the rigidity of CNCs. Meanwhile, the sponge gained superhydrophilic/underwater superhydrophobic wettability with an underwater oil contact angle of up to 165.7° due to its ordered micro/nanoscale structure. B-CNC sheets displayed high oil/water selectivity without additional material doping or chemical modification. For oil/water mixtures, high separation fluxes of approximately 100,000 L·m-2·h-1 and separation efficiencies of up to 99.99 % were obtained. For a Tween 80-stabilized toluene-in-water emulsion, the flux reached >50,000 L·m-2·h-1, and the separation efficiency was above 99.7 %. B-CNC sponge sheets showed significantly higher fluxes and separation efficiencies than other bio-based two-dimensional materials. This research provides a facile and straightforward fabrication method of environmental-friendly B-CNC sponges for rapid, selective oil/water separation.

Back-to-Back Comparison of Third-Generation Sequencing and Next-Generation Sequencing in Carrier Screening of Thalassemia.

CONTEXT.­: Recently, new technologies, such as next-generation sequencing and third-generation sequencing, have been used in carrier screening of thalassemia. However, there is no direct comparison between the 2 methods in carrier screening of thalassemia. OBJECTIVE.­: To compare the clinical performance of third-generation sequencing with next-generation sequencing in carrier screening of thalassemia. DESIGN.­: Next-generation sequencing and third-generation sequencing were simultaneously conducted for 1122 individuals in Hainan Province. RESULTS.­: Among 1122 genetic results, 1105 (98.48%) were concordant and 17 (1.52%) were discordant between the 2 methods. Among the 17 discordant results, 4 were common thalassemia variants, 9 were rare thalassemia variants, and 4 were variations with unknown pathogenicity. Sanger sequencing and polymerase chain reaction for discordant samples confirmed all the results of third-generation sequencing. Among the 685 individuals with common and rare thalassemia variants detected by third-generation sequencing, 512 (74.74%) were carriers of α-thalassemia, 110 (16.06%) were carriers of ß-thalassemia, and 63 (9.20%) had coinheritance of α-thalassemia and ß-thalassemia. Three thalassemia variants were reported for the first time in Hainan Province, including -THAI, -α2.4, and ααααanti3.7. Eleven variants with potential pathogenicity were identified in 36 patients with positive hemoglobin test results. Among 52 individuals with negative hemoglobin test results, 17 were identified with thalassemia variants. In total, third-generation sequencing and next-generation sequencing correctly detected 763 and 746 individuals with variants, respectively. Third-generation sequencing yielded a 2.28% (17 of 746) increment compared with next-generation sequencing. CONCLUSIONS.­: Third-generation sequencing was demonstrated to be a more accurate and reliable approach in carrier screening of thalassemia compared with next-generation sequencing.

Single-cell profiling of the microenvironment in decidual tissue from women with missed abortions.

OBJECTIVE: To define the decidual microenvironment in euploid and aneuploid missed abortions and elective termination of pregnancies. DESIGN: Prospective, multicenter, observational study. SETTING: Tertiary hospital and descriptive analysis of transcriptomic data. PATIENT(S): A total of 34 patients experienced abortions, including 6 women who underwent elective terminations of pregnancy of unplanned pregnancies and 28 cases with missed abortions. All patients underwent their operations from Sep, 2021 to Sep, 2022. INTERVENTION(S): All women underwent villous copy number variation sequencing. Meanwhile, single-cell RNA sequencing were performed in the decidual tissues of 16 women, and reverse transcription quantitative polymerase chain reaction were performed in the decidual tissues of 18 women. MAIN OUTCOME MEASURE(S): Single-cell RNA sequencing was used to explore the changes in the microenvironment of decidual tissues in abortions. RESULT(S): Single-cell RNA sequencing indicated that the microenvironment of the decidual tissue of the missed-abortion group was altered, and that the stromal cells (SCs), natural killer cells, macrophages, and epithelial cells all reflected functional imbalances compared with the elective terminations of pregnancy group. We also noted a correlation between the proportion of senescent SCs and chromosomal abnormalities in missed-abortion embryos. The proportion of senescent decidual SCs in the decidual tissue of missed-abortion patients with common chromosomal abnormalities of the fetus was higher, and this was not conducive to fetal growth and was closely related to missed abortion. In addition, we ascertained that the strength of the HLA-KIR interaction between NK1 and NK2 subsets and non-senescent stromal cell subsets in the missed abortion decidual tissues was weakened, potentially playing a role in the occurrence of missed abortion. CONCLUSION(S): The decidualization of SCs in the missed-abortion decidual tissues was impaired, the clearance of senescent SCs by NK cells was weakened, the killing toxicity of non-senescent SCs was enhanced, macrophages were insufficiently resident at the maternal-fetal interface, and epithelial cell differentiation was unbalanced-all creating a maternal microenvironment that was not conducive to fetal growth. We posit that interfering with the expression of dysregulated genes in the missed-abortion decidual tissues and reversing the maternal microenvironment might constitute an effective means toward improving the clinical outcome of missed abortions. Intriguingly, we observed a correlation between stromal cell senescence and embryonic chromosomal abnormalities. Thus, we hypothesize that the DIO2 marker of senescent SCs can be used as a risk indicator for the occurrence of missed miscarriages with chromosomal abnormalities of the embryos, and that it can be applied to guide the clinical diagnosis and treatment of recurrent abortion. CLINICAL TRIAL REGISTRATION NUMBER: NCT04425317.

Efficient removal of Phaeocystis globosa from seawater with the persulfate activation by arbutin-modified cellulose nanocrystals.

Harmful algal blooms (HABs) from seawater have a severe threat to human health, aquaculture, and coastal nuclear power safety. Thus, it is highly desirable to explore environmentally friendly, efficient, and economic methods for controlling HABs. Herein, the arbutin-modified cellulose nanocrystals (AT-CNC) activated persulfate (PS), as a novel heterogeneous Fenton-like process, was proposed to remove Phaeocystis globosa (P. globosa) from seawater. The AT-CNC was synthesized via the surface modification of AT on CNC. The effects of AT dosage, CNC dosage, and PS dosage on the removal performance of P. globosa were investigated. With the addition of 530 mg/L AT-CNC (6 wt% AT/CNC of AT loading) and 120 mg/L PS, the removal percentage of chlorophyll a (Rc), optical density at 680 nm (Ro) and turbidity (Rt) reached 97.7%, 91.9% and 85.2% at 24 h. According to electron paramagnetic resonance (EPR) spectra and radical quenching tests, the predominant free radicals inactivating P. globosa were hydroxyl radicals (•OH). Additionally, the flocculation of the inactivated algae cells by AT-CNC was also critical for removing P. globosa. Moreover, a positive environmental impact was achieved in the AT-CNC-PS system due to the reduction of nitrogen, phosphorus and organic carbon contents. Based on the excellent removal performance for P. globosa, we believe that the AT-CNC activated persulfate is a promising option for HABs control.

High accuracy of single-molecule real-time sequencing in detecting a rare α-globin fusion gene in carrier screening population.

INTRODUCTION: The α-globin fusion gene between the HBA2 and HBAP1 genes becomes clinically important in thalassemia screening because this fusion gene can cause severe hemoglobin (Hb) H disease when combining with α0 -thalassemia (α0 -thal). Due to its uncommon rearrangement in the α gene cluster without dosage changes, this fusion gene is undetectable by common molecular testing approaches used for α-thal diagnosis. METHODS: In this study, we used the single-molecule real-time (SMRT) sequencing technique to detect this fusion gene in 23 carriers identified by next-generation sequencing (NGS) among 16,504 screened individuals. Five primers for α and ß thalassemia were utilized. RESULTS: According to the NGS results, the 23 carriers include 14 pure heterozygotes, eight compound heterozygotes with common α-thal alleles, and one homozygote. By using SMRT, the fusion mutant was successfully detected in all 23 carriers. Furthermore, SMRT corrected the diagnosis in two "pure" heterozygotes: one was compound heterozygote with anti-3.7 triplication, and the other was homozygote. CONCLUSION: Our results indicate that SMRT is a superior method compared to NGS in detecting the α fusion gene, attributing to its efficient, accurate, and one-step properties.