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1.
Adv Mater ; 36(21): e2313134, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38331419

RESUMO

The barrier structure is designed to enhance the operating temperature of the infrared detector, thereby improving the efficiency of collecting photogenerated carriers and reducing dark current generation, without suppressing the photocurrent. However, the development of barrier detectors using conventional materials is limited due to the strict requirements for lattice and band matching. In this study, a high-performance unipolar barrier detector is designed utilizing a black arsenic phosphorus/molybdenum disulfide/black phosphorus van der Waals heterojunction. The device exhibits a broad response bandwidth ranging from visible light to mid-wave infrared (520 nm to 4.6 µm), with a blackbody detectivity of 2.7 × 1010 cmHz-1/2 W-1 in the mid-wave infrared range at room temperature. Moreover, the optical absorption anisotropy of black arsenic phosphorus enables polarization resolution detection, achieving a polarization extinction ratio of 35.5 at 4.6 µm. Mid-wave infrared imaging of the device is successfully demonstrated at room temperature, highlighting the significant potential of barrier devices based on van der Waals heterojunctions in mid-wave infrared detection.

2.
J Chem Theory Comput ; 20(5): 1811-1820, 2024 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-38320113

RESUMO

Polar molecules are a promising platform for achieving scalable quantum information processing because of their long-range electric dipole-dipole interactions. Here, we take the coupled ultracold CaF molecules in an external electric field with gradient as qubits and concentrate on the creation of intermolecular entanglement with the method of deep reinforcement learning (RL). After sufficient training episodes, the educated RL agents can discover optimal time-dependent control fields that steer the molecular systems from separate states to two-qubit and three-qubit entangled states with high fidelities. We analyze the fidelities and the negativities (characterizing entanglement) of the generated states as a function of training episodes. Moreover, we present the population dynamics of the molecular systems under the influence of control fields discovered by the agents. Compared with the schemes for creating molecular entangled states based on optimal control theory, some conditions (e.g., molecular spacing and electric field gradient) adopted in this work are more feasible in the experiment. Our results demonstrate the potential of machine learning to effectively solve quantum control problems in polar molecular systems.

3.
Adv Sci (Weinh) ; 11(12): e2307396, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38225755

RESUMO

The utilization of hot carriers as a means to surpass the Shockley-Queasier limit represents a promising strategy for advancing highly efficient photovoltaic devices. Quantum dots, owing to their discrete energy states and limited multi-phonon cooling process, are regarded as one of the most promising materials. However, in practical implementations, the presence of numerous defects and discontinuities in colloidal quantum dot (CQD) films significantly curtails the transport distance of hot carriers. In this study, the harnessing of excess energies from hot-carriers is successfully demonstrated and a world-record carrier diffusion length of 15 µm is observed for the first time in colloidal systems, surpassing existing hot-carrier materials by more than tenfold. The observed phenomenon is attributed to the specifically designed honeycomb-like topological structures in a HgTe CQD superlattice, with its long-range periodicity confirmed by High-Resolution Transmission Electron Microscopy(HR-TEM), Selected Area Electron Diffraction(SAED) patterns, and low-angle X-ray diffraction (XRD). In such a superlattice, nonlocal hot carrier transport is supported by three unique physical properties: the wavelength-independent responsivity, linear output characteristics and microsecond fast photoresponse. These findings underscore the potential of HgTe CQD superlattices as a feasible approach for efficient hot carrier collection, thereby paving the way for practical applications in highly sensitive photodetection and solar energy harvesting.

4.
Adv Sci (Weinh) ; 11(6): e2307169, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38044286

RESUMO

The realization of a controllable transparent conducting system with selective light transparency is crucial for exploring many of the most intriguing effects in top-illuminated optoelectronic devices. However, the performance is limited by insufficient electrical conductivity, low work function, and vulnerable interface of traditional transparent conducting materials, such as tin-doped indium oxide. Here, it is reported that two-dimensional (2D) titanium carbide (Ti3 C2 Tx ) MXene film acts as an efficient transparent conducting electrode for the lead sulfide (PbS) colloidal quantum dots (CQDs) photodiode with controllable near infrared transmittance. The solution-processed interface engineering of MXene and PbS layers remarkably reduces the interface defects of MXene/PbS CQDs and the carrier concentration in the PbS layer. The stable Ti3 C2 Tx /PbS CQDs photodiodes give rise to a high specific detectivity of 5.51 × 1012  cm W-1  Hz1/2 , a large dynamic response range of 140 dB, and a large bandwidth of 0.76 MHz at 940 nm in the self-powered state, ranking among the most exceptional in terms of comprehensive performance among reported PbS CQDs photodiodes. In contrast with the traditional photodiode technologies, this efficient and stable approach opens a new horizon to construct widely used infrared photodiodes with CQDs and MXenes.

5.
J Colloid Interface Sci ; 638: 709-718, 2023 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-36780851

RESUMO

Among many supercapacitor electrode materials, carbon materials are widely used due to their large specific surface area, good electrical conductivity and high economic efficiency. However, carbon-based supercapacitors face the challenges of low energy density and limited operating environment. This work reports a facile self-assembled method to prepare three-dimensional carbon nanotubes/reduced graphene oxide (CNTs/rGO) aerogel material, which was applied as both positive and negative electrodes in a symmetric superacapacitor. The fabricated supercapacitor exhibited prominent capacitive performance not only at room temperature, but also at extreme temperatures (-20 âˆ¼ 80 °C). The specific capacitances of the symmetric supercapacitors based on CNTs/rGO at a weight ratio of 2:5 respectively reached 107.8 and 128.2 F g-1 at 25 °C and 80 °C with KOH as the electrolyte, and 80.0 and 144.6 F g-1 at -20 °C and 60 °C with deep eutectic solvent as the electrolyte. Notably, the capacitance retention and coulombic efficiency of the assembled supercapacitors remained almost unchanged after 20,000 cycles of charge/discharge test over a wide temperature range. The work uncovered a possibility for the development of high-performance supercapacitors flexibly operated at extreme temperatures.

6.
Bioresour Technol ; 372: 128654, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36682475

RESUMO

Based on the semi-continuous anaerobic co-digestion (AcoD) reactor, the effects of biochar addition on the internal environmental changes and gas production characteristics were studied under the condition of biogas slurry recirculation. The results showed that the addition of biochar enhanced the degradation and metabolic pathways of acetate and propionate, thereby reducing the concentrations of volatile fatty acids (VFAs), total ammonia and chemical oxygen demand by 55 %, 41 % and 61 %, respectively. The buffer system formed by the combination of NH4+ and VFAs of C2-C5 was also enhanced, thereby improving the stability of the system. The addition of biochar effectively increased the relative abundance of Bacteroidetes, Chloroflexi, Spirochaetota and Synergistota, and enhanced three methanogenic metabolic pathways. This study provides scientific support for the application of biochar to solve the system inhibition in mixed substrate semi-continuous AcoD process and provides technical support for the stable operation of biogas project.


Assuntos
Biocombustíveis , Esterco , Animais , Suínos , Anaerobiose , Zea mays/metabolismo , Reatores Biológicos , Metano/metabolismo , Ácidos Graxos Voláteis/metabolismo , Digestão
7.
J Virol ; 96(24): e0149222, 2022 12 21.
Artigo em Inglês | MEDLINE | ID: mdl-36468862

RESUMO

Bovine viral diarrhea virus (BVDV) is the etiologic agent of bovine viral diarrhea-mucosal disease, one of the most important viral diseases of cattle, leading to numerous losses to the cattle rearing industry worldwide. The pathogenicity of BVDV is extremely complex, and many underlying mechanisms involved in BVDV-host interactions are poorly understood, especially how BVDV utilizes host metabolism pathway for efficient viral replication and spread. In our previous study, using an integrative analysis of transcriptomics and proteomics, we found that DHCR24 (3ß-hydroxysteroid-Δ24 reductase), a key enzyme in regulating cholesterol synthesis, was significantly upregulated at both gene and protein levels in the BVDV-infected bovine cells, indicating that cholesterol is important for BVDV replication. In the present study, the effects of DHCR24-mediated cholesterol synthesis on BVDV replication was explored. Our results showed that overexpression of the DHCR24 effectively promoted cholesterol synthesis, as well as BVDV replication, while acute cholesterol depletion in the bovine cells by treating cells with methyl-ß-cyclodextrin (MßCD) obviously inhibited BVDV replication. In addition, knockdown of DHCR24 (gene silencing with siRNA targeting DHCR24, siDHCR24) or chemical inhibition (treating bovine cells with U18666A, an inhibitor of DHCR24 activity and cholesterol synthesis) significantly suppressed BVDV replication, whereas supplementation with exogenous cholesterol to the siDHCR24-transfected or U18666A-treated bovine cells remarkably restored viral replication. We further confirmed that BVDV nonstructural protein NS5A contributed to the augmentation of DHCR24 expression. Conclusively, augmentation of the DHCR24 induced by BVDV infection plays an important role in BVDV replication via promoting cholesterol production. IMPORTANCE Bovine viral diarrhea virus (BVDV), an important pathogen of cattle, is the causative agent of bovine viral diarrhea-mucosal disease, which causes extensive economic losses in both cow- and beef-rearing industry worldwide. The molecular interactions between BVDV and its host are extremely complex. In our previous study, we found that an essential host factor 3ß-hydroxysteroid-δ24 reductase (DHCR24), a key enzyme involved in cholesterol synthesis, was significantly upregulated at both gene and protein levels in BVDV-infected bovine cells. Here, we experimentally explored the function of the DHCR24-mediated cholesterol synthesis in regulating BVDV replication. We elucidated that the augmentation of the DHCR24 induced by BVDV infection played a significant role in viral replication via promoting cholesterol synthesis. Our data provide evidence that BVDV utilizes a host metabolism pathway to facilitate its replication and spread.


Assuntos
Doença das Mucosas por Vírus da Diarreia Viral Bovina , Colesterol , Vírus da Diarreia Viral Bovina , Oxirredutases atuantes sobre Doadores de Grupo CH-CH , Replicação Viral , Animais , Bovinos , Colesterol/biossíntese , Vírus da Diarreia Viral Bovina/genética , Vírus da Diarreia Viral Bovina/fisiologia , Oxirredutases atuantes sobre Doadores de Grupo CH-CH/genética , Células Cultivadas
9.
Nat Commun ; 13(1): 3198, 2022 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-35680950

RESUMO

With the further miniaturization and integration of multi-dimensional optical information detection devices, polarization-sensitive photodetectors based on anisotropic low-dimension materials have attractive potential applications. However, the performance of these devices is restricted by intrinsic property of materials leading to a small polarization ratio of the detectors. Here, we construct a black phosphorus (BP) homojunction photodetector defined by ferroelectric domains with ultra-sensitive polarization photoresponse. With the modulation of ferroelectric field, the BP exhibits anisotropic dispersion changes, leading an increased photothermalelectric (PTE) current in the armchair (AC) direction. Moreover, the PN junction can promote the PTE current and accelerate carrier separation. As a result, the BP photodetector demonstrates an ultrahigh polarization ratio (PR) of 288 at 1450 nm incident light, a large photoresponsivity of 1.06 A/W, and a high detectivity of 1.27 × 1011 cmHz1/2W-1 at room temperature. This work reveals the great potential of BP in future polarized light detection.

10.
Sci Adv ; 8(19): eabn1811, 2022 05 13.
Artigo em Inglês | MEDLINE | ID: mdl-35544556

RESUMO

New-generation infrared detectors call for higher operation temperature and polarization sensitivity. For traditional HgCdTe infrared detectors, the additional polarization optics and cryogenic cooling are necessary to achieve high-performance infrared polarization detection, while they can complicate this system and limit the integration. Here, a mixed-dimensional HgCdTe/black phosphorous van der Waals heterojunction photodiode is proposed for polarization-sensitive midwave infrared photodetection. Benefiting from van der Waals integration, type III broken-gap band alignment heterojunctions are achieved. Anisotropy optical properties of black phosphorous bring polarization sensitivity from visible light to midwave infrared without external optics. Our devices show an outstanding performance at room temperature without applied bias, with peak blackbody detectivity as high as 7.93 × 1010 cm Hz1/2 W-1 and average blackbody detectivity over 2.1 × 1010 cm Hz1/2 W-1 in midwave infrared region. This strategy offers a possible practical solution for next-generation infrared detector with high operation temperature, high performance, and multi-information acquisition.

11.
RSC Adv ; 12(8): 4939-4945, 2022 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-35425495

RESUMO

Flexible optoelectronic devices have numerous applications in personal wearable devices, bionic detectors, and other systems. There is an urgent need for functional materials with appealing electrical and optoelectronic properties, stretchable electrodes with outstanding mechanical flexibility, and gate medium with flexibility and low power consumption. Two-dimensional transition metal dichalcogenides (TMDCs), a novel kind of widely studied optoelectrical material, have good flexibility for their ultrathin nature. P(VDF-TrFE) is a kind of organic material with good flexibility which has been proved to be a well-performing ferroelectric gate material for photodetectors. Herein, we directly fabricated a well-performing photodetector based on ReS2 and P(VDF-TrFE) on a flexible substrate. The device achieved a high responsivity of 11.3 A W-1 and a high detectivity of 1.7 × 1010 Jones from visible to near-infrared. Moreover, with strain modulation, the device's responsivity improved 2.6 times, while the detectivity improved 1.8 times. This research provides a prospect of flexible photodetectors in the near-infrared wavelength.

12.
Front Immunol ; 13: 862828, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35371109

RESUMO

Bovine viral diarrhea virus (BVDV) is the causative agent of bovine viral diarrhea-mucosal disease (BVD-MD), an important viral disease in cattle that is responsible for extensive economic losses to the cattle industry worldwide. Currently, several underlying mechanisms involved in viral replication, pathogenesis, and evading host innate immunity of BVDV remain to be elucidated, particularly during the early stage of virus infection. To further explore the mechanisms of BVDV-host interactions, the transcriptomics and proteomics profiles of BVDV-infected MDBK cells were sequenced using RNA-seq and iTRAQ techniques, respectively, and followed by an integrative analysis. Compared with mock-infected MDBK cells, a total of 665 differentially expressed genes (DEGs) (391 down-regulated, 274 up-regulated) and 725 differentially expressed proteins (DEPs) (461 down-regulated, 264 up-regulated) were identified. Among these, several DEGs and DEPs were further verified using quantitative RT-PCR and western blot. Following gene ontology (GO) annotation and KEGG enrichment analysis, we determined that these DEGs and DEPs were significantly enriched in multiple important cellular signaling pathways including NOD-like receptor, Toll-like receptor, TNF, NF-κB, MAPK, cAMP, lysosome, protein processing in endoplasmic reticulum, lipid metabolism, and apoptosis signaling pathways. Significantly, the down-regulated DEGs and DEPs were predominantly associated with apoptosis-regulated elements, inflammatory factors, and antiviral elements that were involved in innate immunity, thus, indicating that BVDV could inhibit apoptosis and the expression of host antiviral genes to facilitate viral replication. Meanwhile, up-regulated DEGs and DEPs were primarily involved in metabolism and autophagy signaling pathways, indicating that BVDV could utilize the host metabolic resources and cell autophagy to promote replication. However, the potential mechanisms BVDV-host interactions required further experimental validation. Our data provide an overview of changes in transcriptomics and proteomics profiles of BVDV-infected MDBK cells, thus, providing an important basis for further exploring the mechanisms of BVDV-host interactions.


Assuntos
Vírus da Diarreia Viral Bovina , Transcriptoma , Animais , Antivirais , Bovinos , Diarreia , Vírus da Diarreia Viral Bovina/genética , Interações entre Hospedeiro e Microrganismos , Proteômica
13.
J Colloid Interface Sci ; 611: 599-608, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-34973656

RESUMO

The development of efficient and robust non-precious electrocatalysts for water oxidation at a mild condition is extremely desirable for industrial water splitting. Herein we developed a facile solvothermal strategy to synthesize cobalt metal organic frameworks (Co-MOFs) with sheet-like structure, which showed highly promising performance for electrocatalytic oxygen evolution. The best Co-MOF sample afforded an ultra-high oxygen evolution current density of 63.4 mA cm-2 at 1.75 V in 1 M KOH with a catalyst loading of only 0.21 mg cm-2. Notably, its electrochemical performance remained unchanged after 10,000 cyclic voltammograms indicating very promising long-term stability. Detailed study of the mechanism of the oxygen evolution by density functional theory (DFT) indicated that the strong π-conjugation formed between the central cobalt ion and adjacent aromatic rings favored the high electrocatalytic performance. The solvothermally synthesized MOFs proposed in this paper are expected to inspire the rational design of high-performance electrocatalysts for water oxidation with atomic and molecular level structural control and the exploration of structure-performance relationships to understand the electrocatalytic origin.

14.
J Virol ; 95(12)2021 05 24.
Artigo em Inglês | MEDLINE | ID: mdl-33827944

RESUMO

Koi herpesvirus (KHV) is highly contagious and lethal to cyprinid fish, causing significant economic losses to the carp aquaculture industry, particularly to koi carp breeders. Vaccines delivered through intramuscular needle injection or gene gun are not suitable for mass vaccination of carp. So, the development of cost-effective oral vaccines that are easily applicable at a farm level is highly desirable. In this study, we utilized chitosan-alginate capsules as an oral delivery system for a live probiotic (Lactobacillus rhamnosus) vaccine, pYG-KHV-ORF81/LR CIQ249, expressing KHV ORF81 protein. The tolerance of the encapsulated recombinant Lactobacillus to various digestive environments and the ability of the probiotic strain to colonize the intestine of carp was tested. The immunogenicity and the protective efficacy of the encapsulated probiotic vaccine was evaluated by determining IgM levels, lymphocyte proliferation, expression of immune-related genes, and viral challenge to vaccinated fish. It was clear that the chitosan-alginate capsules protected the probiotic vaccine effectively against extreme digestive environments, and a significant level (P < 0.01) of antigen-specific IgM with KHV-neutralizing activity was detected, which provided a protection rate of ca. 85% for koi carp against KHV challenge. The strategy of using chitosan-alginate capsules to deliver probiotic vaccines is easily applicable for mass oral vaccination of fish.IMPORTANCE An oral probiotic vaccine, pYG-KHV-ORF81/LR CIQ249, encapsulated by chitosan-alginate capsules as an oral delivery system was developed for koi carp against koi herpesvirus (KHV) infection. This encapsulated probiotic vaccine can be protected from various digestive environments and maintain effectively high viability, showing a good tolerance to digestive environments. This encapsulated probiotic vaccine has a good immunogenicity in koi carp via oral vaccination, and a significant level of antigen-specific IgM was effectively induced after oral vaccination, displaying effective KHV-neutralizing activity. This encapsulated probiotic vaccine can provide effective protection for koi carp against KHV challenge, which is handling-stress free for the fish, cost effective, and suitable for the mass oral vaccination of koi carp at a farm level, suggesting a promising vaccine strategy for fish.


Assuntos
Carpas , Doenças dos Peixes/prevenção & controle , Infecções por Herpesviridae/veterinária , Herpesviridae/imunologia , Vacinas contra Herpesvirus/administração & dosagem , Probióticos , Proteínas Virais/imunologia , Administração Oral , Alginatos , Animais , Anticorpos Neutralizantes/sangue , Anticorpos Antivirais/sangue , Antígenos Virais/imunologia , Cápsulas , Proliferação de Células , Quitosana , Infecções por Herpesviridae/prevenção & controle , Vacinas contra Herpesvirus/imunologia , Imunogenicidade da Vacina , Imunoglobulina M/sangue , Lacticaseibacillus rhamnosus , Linfócitos/fisiologia , Vacinação em Massa/veterinária , Proteínas Recombinantes de Fusão , Baço/imunologia , Baço/metabolismo , Vacinas Sintéticas/administração & dosagem , Proteínas Virais/genética
15.
Arch Virol ; 166(3): 831-840, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33486631

RESUMO

Ovine pulmonary adenomatosis (OPA) is caused by jaagsiekte sheep retrovirus (JSRV) and is a chronic, progressive, and infectious neoplastic lung disease in sheep, which causes significant economic losses to the sheep industry. Neither a vaccine nor serological diagnostic methods to detect OPA are available. We performed a JSRV infection survey in sheep using blood samples (n = 1,372) collected in the three northeastern provinces of China (i.e., Inner Mongolia, Heilongjiang, and Jilin) to determine JSRV infection status in sheep herds using a real-time PCR assay targeting the gag gene of JSRV. The ovine endogenous retrovirus sequence was successfully amplified in all sheep samples tested (296 from the Inner Mongolia Autonomous Region, 255 from Jilin province, and 821 from Heilongjiang province). Subsequently, we attempted to distinguish exogenous JSRV (exJSRV) and endogenous JSRV (enJSRV) infections in these JSRV-positive samples using a combination assay that identifies a ScaI restriction site in an amplified 229-bp fragment of the gag gene of JSRV and a "LHMKYXXM" motif in the cytoplasmic tail region of the JSRV envelope protein. The ScaI restriction site is present in all known oncogenic JSRVs but absent in ovine endogenous retroviruses, while the "LHMKYXXM" motif is in all known exJSRVs but not in enJSRVs. Interestingly, one JSRV strain (HH13) from Heilongjiang province contained the "LHMKYXXM" motif but not the ScaI enzyme site. Phylogenetic analysis showed that strain HH13 was closely related to strain enJSRV-21 reported in the USA, indicating that HH13 could be an exogenous virus. Our results provide valuable information for further research on the genetic evolution and pathogenesis of JSRV.


Assuntos
Retrovirus Endógenos/genética , Produtos do Gene env/genética , Retrovirus Jaagsiekte de Ovinos/genética , Adenomatose Pulmonar Ovina/epidemiologia , Adenomatose Pulmonar Ovina/patologia , Motivos de Aminoácidos/genética , Animais , Sequência de Bases , China/epidemiologia , DNA Viral/análise , Desoxirribonucleases de Sítio Específico do Tipo II/metabolismo , Evolução Molecular , Genoma Viral/genética , Retrovirus Jaagsiekte de Ovinos/classificação , Filogenia , Reação em Cadeia da Polimerase em Tempo Real , Mapeamento por Restrição , Homologia de Sequência do Ácido Nucleico , Ovinos
16.
Microb Cell Fact ; 19(1): 186, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-33004035

RESUMO

BACKGROUND: Bovine viral diarrhea virus (BVDV) is one of the main causes of infectious diseases in cattle and causes large financial losses to the cattle industry worldwide. In this study, Lactobacillus casei strain W56 (Lc W56) was used as antigen deliver carrier to construct a recombinant Lactobacillus vaccine pPG-E2-ctxB/Lc W56 constitutively expressing BVDV E2 protein fused with cholera toxin B subunit (ctxB) as an adjuvant, and its immunogenicity against BVDV infection in mice model by oral route was explored. RESULTS: Our results suggested that pPG-E2-ctxB/Lc W56 can effectively activate dendritic cells (DCs) in the Peyer's patches, up-regulate the expression of Bcl-6, and promote T-follicular helper (Tfh) cells differentiation, as well as enhance B lymphocyte proliferation and promote them differentiate into specific IgA-secreting plasma cells, secreting anti-E2 mucosal sIgA antibody with BVDV-neutralizing activity. Moreover, significant levels (p < 0.01) of BVDV-neutralizing antigen-specific serum antibodies were induced in the pPG-E2-ctxB/LC W56 group post-vaccination. The recombinant Lactobacillus vaccine can induce cellular immune responses, and significant levels (p < 0.01) of Th1-associated cytokines (IL-2, IL-12, and IFN-γ), Th2-associated cytokines (IL-4, IL-10) and Th17-associated cytokine (IL-17) were determined in the serum of vaccinated mice. Significantly, the recombinant Lactobacillus vaccine provides immune protection against BVDV infection, which can be cleared effectively by the vaccine post-challenge in orally vaccinated animals. CONCLUSIONS: The genetically engineered Lactobacillus vaccine constructed in this study is immunogenic in mice and can induce mucosal, humoral, and cellular immune responses, providing effective anti-BVDV immune protection. It thus represents a promising strategy for vaccine development against BVDV.


Assuntos
Doença das Mucosas por Vírus da Diarreia Viral Bovina/prevenção & controle , Toxina da Cólera/imunologia , Vírus da Diarreia Viral Bovina/imunologia , Lacticaseibacillus casei/imunologia , Proteínas do Envelope Viral/imunologia , Vacinas Virais/imunologia , Adjuvantes Imunológicos , Administração Oral , Animais , Anticorpos Neutralizantes/sangue , Anticorpos Antivirais/sangue , Formação de Anticorpos/imunologia , Doença das Mucosas por Vírus da Diarreia Viral Bovina/patologia , Bovinos , Citocinas/imunologia , Trato Gastrointestinal/patologia , Trato Gastrointestinal/virologia , Imunidade Celular , Lacticaseibacillus casei/genética , Camundongos , Camundongos Endogâmicos BALB C , Proteínas Recombinantes de Fusão/imunologia , Organismos Livres de Patógenos Específicos , Vacinas Sintéticas/imunologia , Carga Viral
17.
Nanoscale ; 12(17): 9628-9639, 2020 May 07.
Artigo em Inglês | MEDLINE | ID: mdl-32319453

RESUMO

The commercialization of metal-air batteries requires efficient, low-cost, and stable bifunctional electrocatalysts for reversible electrocatalysis of the oxygen reduction reaction (ORR) and the oxygen evolution reaction (OER). The modification of natural coal by heteroatoms such as N and S, or metal oxide species, has been demonstrated to form very promising electrocatalysts for the ORR and OER. However, it remains elusive and underexplored as to how the impurity elements in coal may impact the electrocatalytic properties of coal-derived catalysts. Herein, we explore the influence of the presence of various trace metals that are notable impurities in coal, including Al, Si, Ca, K, Fe, Mg, Co, Mn, Ni, and Cu, on the electrochemical performance of the prepared catalysts. The constructed Zn-air batteries are further shown to be able to power green LED lights for more than 80 h. The charge-discharge polarization curves exhibited excellent and durable rechargeability over 500 (ca. 84 h) continuous cycles. The promotional effect of the trace elements is believed to accrue from a combination of electronic structure modification of the active sites, enhancement of the active site density, and formation of a conductive 3-dimensional hierarchical network of carbon nanotubes.

18.
Vaccines (Basel) ; 8(1)2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31936328

RESUMO

Clostridium perfringens is an opportunistic pathogen. Its main virulence factors are exotoxins, which are the etiological agents of enteritis necroticans and enterotoxemia caused in livestock (cattle, sheep, and rabbits). Here, we demonstrated effective immune protection for rabbits against α, ß, and ε exotoxins of C. perfringens provided by an oral tetravalent bait probiotic vaccine delivering α, ε, ß1, and ß2 toxoids of C. perfringens. Results showed that the recombinant probiotic had good segregational stability and good colonization ability in the rabbit intestinal tract. Oral administration of the probiotic vaccine can effectively elicit significant levels of antigen-specific mucosa sIgA and sera IgG antibodies with exotoxin-neutralizing activity. Additionally, oral immunization with the probiotic vaccine effectively promoted lymphoproliferation and Th1/Th2-associated cytokine production. The protection rate of immunized rabbits with the probiotic vaccine was 80% after challenging rabbits with a combination of C. perfringens (toxinotypes A, C, and D) and exotoxin mixture, which was better than the 60% provided by a commercial inactivated C. perfringens A, C, and D trivalent vaccine. Moreover, obvious histopathological changes were observed in the intestinal tissues of rabbits in the commercial vaccine and PBS groups. The bait probiotic vaccine can provide effective protection against C. perfringens exotoxins, suggesting a promising C. perfringens vaccination strategy.

19.
Waste Manag ; 92: 59-67, 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-31160027

RESUMO

Insight to nitrogen transformation and cycling during composting is vital in developing management strategies that improve nitrogen content and quality of the end product. In this study, a positive ventilation device was constructed and used to elucidate nitrogen transformation and microbial community structures during the composting of cow manure and rice straw. Bacterial community successions were analyzed during the composting process by examining the change in their structural dynamics using high-throughput sequencing technique. The results revealed that dominant phyla, included Acidobacter, Proteobacteria, Firmicutes, Bacteroidetes, Chloroflexi, and Actinobacteria. Furthermore, a positive strong correlation was observed between the key bacterial communities and nitrogen transformation. Analyses of functional genera, Spearman correlation and Path showed that Thermomonospora_curvata_DSM_43183 followed by Luteimonas and Simiduia, Brevundimonas and Tamlana, Pseudomonas followed by Brevundimonas and Flavobacterium were the key bacterial communities affecting NH4+-N, NO3--N, and NO2--N transformation, respectively. Thauera followed by Pseudomonas_putida_NBRC_14164 played a dominant role in N2O transformation.


Assuntos
Compostagem , Microbiota , Animais , Bovinos , Feminino , Sequenciamento de Nucleotídeos em Larga Escala , Esterco , Nitrogênio , Solo
20.
Appl Opt ; 58(7): 1733-1738, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30874208

RESUMO

A passively mode-locked Er-doped fiber laser with a fundamental repetition rate of 2.68 GHz is reported. The oscillator operating at a central wavelength of 1558.35 nm has a compact, robust structure and low-noise performance. The timing jitter integrated from 30 MHz down to 300 Hz is 82.5 fs, and the timing jitter performance is analyzed based on the theory model. The amplification and compression of the high repetition rate optical pulses are also investigated. After a three-stage amplifier, the average power is boosted to 430 mW. Meanwhile, based on the nonlinear self-phase modulation effect, the spectral bandwidth is broadened from 7.56 to 19.2 nm, and the corresponding pulse width is compressed to 244 fs.

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