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1.
J Biomed Opt ; 29(7): 076003, 2024 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-38989529

RESUMO

Significance: Tissues' biomechanical properties, such as elasticity, are related to tissue health. Optical coherence elastography produces images of tissues based on their elasticity, but its performance is constrained by the laser power used, working distance, and excitation methods. Aim: We develop a new method to reconstruct the elasticity contrast image over a long working distance, with only low-intensity illumination, and by non-contact acoustic wave excitation. Approach: We combine single-photon vibrometry and quantum parametric mode sorting (QPMS) to measure the oscillating backscattered signals at a single-photon level and derive the phantoms' relative elasticity. Results: We test our system on tissue-mimicking phantoms consisting of contrast sections with different concentrations and thus stiffness. Our results show that as the driving acoustic frequency is swept, the phantoms' vibrational responses are mapped onto the photon-counting histograms from which their mechanical properties-including elasticity-can be derived. Through lateral and longitudinal laser scanning at a fixed frequency, a contrast image based on samples' elasticity can be reliably reconstructed upon photon level signals. Conclusions: We demonstrated the reliability of QPMS-based elasticity contrast imaging of agar phantoms in a long working distance, low-intensity environment. This technique has the potential for in-depth images of real biological tissue and provides a new approach to elastography research and applications.


Assuntos
Técnicas de Imagem por Elasticidade , Imagens de Fantasmas , Fótons , Técnicas de Imagem por Elasticidade/métodos , Técnicas de Imagem por Elasticidade/instrumentação , Processamento de Imagem Assistida por Computador/métodos , Elasticidade , Reprodutibilidade dos Testes
2.
Nat Food ; 5(6): 480-490, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38872016

RESUMO

Human milk oligosaccharides (HMOs) are a diverse class of carbohydrates which support the health and development of infants. The vast health benefits of HMOs have made them a commercial target for microbial production; however, producing the approximately 200 structurally diverse HMOs at scale has proved difficult. Here we produce a diversity of HMOs by leveraging the robust carbohydrate anabolism of plants. This diversity includes high-value and complex HMOs, such as lacto-N-fucopentaose I. HMOs produced in transgenic plants provided strong bifidogenic properties, indicating their ability to serve as a prebiotic supplement with potential applications in adult and infant health. Technoeconomic analyses demonstrate that producing HMOs in plants provides a path to the large-scale production of specific HMOs at lower prices than microbial production platforms. Our work demonstrates the promise in leveraging plants for the low-cost and sustainable production of HMOs.


Assuntos
Leite Humano , Oligossacarídeos , Plantas Geneticamente Modificadas , Oligossacarídeos/metabolismo , Humanos , Leite Humano/metabolismo , Leite Humano/química , Plantas Geneticamente Modificadas/genética , Prebióticos , Fotossíntese
3.
Opt Express ; 32(10): 16657-16669, 2024 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-38858866

RESUMO

We demonstrate a single-photon counting Raman spectroscope and benchmark it against conventional and surface-enhanced Raman spectroscopy. For direct comparison without ambiguity, we use the same solutions of Rhodamine 6G and a common optical setup with either a spectrometer or an acousto-optic tunable filter, whereas the surface enhancement is realized with immobilized Ag nanoparticles. Our results find that the single photon counting significantly elevates the detection sensitivity by up to eight orders of magnitude, arriving at a comparable level of surface-enhanced Raman spectroscopy. Another significant advantage is with the time-resolving measurement, where we demonstrate time-gated and time-correlated single-photon counting with sub-nanosecond resolution. It offers insights into the samples' transient responses and enables the isolation of Raman scattering from fluorescence signals.

4.
Opt Lett ; 49(12): 3388-3391, 2024 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-38875627

RESUMO

We demonstrate an invertible all-optical gate on chip, with the roles of control and signal switchable by slightly adjusting their relative arrival time at the gate. It is based on the quantum Zeno blockade (QZB) driven by sum-frequency generation (SFG) in a periodically poled lithium niobate microring resonator. For two nearly identical nanosecond pulses, the later arriving pulse is modulated by the earlier arriving one, resulting in 2.4 and 3.9 power extinction between the two, respectively, when their peak powers are 1 mW and 2 mW, respectively. Our results, while to be improved and enriched, herald a new, to the best of our knowledge, paradigm of logical gates and circuits for exotic applications.

5.
Opt Lett ; 49(9): 2449-2452, 2024 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-38691741

RESUMO

Broadband frequency comb generation through cascaded quadratic nonlinearity remains experimentally untapped in free-space cavities with bulk χ(2) materials mainly due to the high threshold power and restricted ability of dispersion engineering. Thin-film lithium niobate (LN) is a good platform for nonlinear optics due to the tight mode confinement in a nano-dimensional waveguide, the ease of dispersion engineering, large quadratic nonlinearities, and flexible phase matching via periodic poling. Here we demonstrate broadband frequency comb generation through dispersion engineering in a thin-film LN microresonator. Bandwidths of 150 nm (80 nm) and 25 nm (12 nm) for center wavelengths at 1560 and 780 nm are achieved, respectively, in a cavity-enhanced second-harmonic generation (doubly resonant optical parametric oscillator). Our demonstration paves the way for pure quadratic soliton generation, which is a great complement to dissipative Kerr soliton frequency combs for extended interesting nonlinear applications.

6.
Medicine (Baltimore) ; 103(21): e37388, 2024 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-38788037

RESUMO

To investigate the effects and molecular mechanisms of wedelolactone (WEL) on high glucose-induced injury of human retinal vascular endothelial cells (HRECs). The cell injury model was established by incubating HRECs with 30 mmol/L glucose for 24 hour. HRECs were divided into control (Con) group, high glucose (HG) group, HG + WEL-low dose (L) group, HG + WEL-medium dose (M), HG + WEL-high dose (H) group, HG + miR-NC group, HG + miR-190 group, HG + WEL + antimiR-NC group, HG + WEL + antimiR-190 group. The kit detects cellular reactive oxygen species (ROS), superoxide dismutase (SOD), and malondialdehyde (MDA) content; cell apoptosis was analyzed by flow cytometry; miR-190 expression was detected by real-time quantitative PCR (RT-qPCR). Compared with Con group, the levels of ROS and MDA in the HG group were significantly increased (P < .01), the SOD activity and the expression of miR-190 expression were significantly decreased (P < .05), and the apoptosis rate was significantly increased (P < .01). Compared with HG group, the levels of ROS and MDA in HG + WEL-L group, HG + WEL-M group and HG + WEL-H group were significantly decreased (P < .05), SOD activity and miR-190 expression were significantly increased (P < .05), and apoptosis rate was significantly reduced (P < .05). Compared with the HG + miR-NC group, the levels of ROS and MDA in HG + miR-190 group were significantly reduced (P < .01), SOD activity was significantly increased (P < .01), and apoptosis rate was significantly reduced (P < .05). Compared with the HG + WEL + antimiR-NC group, the ROS level and MDA content in the HG + WEL + antimiR-190 group were significantly increased (P < .05), SOD activity was significantly decreased (P < .05), and apoptosis rate was significantly increased (P < .05). Wedelolactone can attenuate high glucose-induced HRECs apoptosis and oxidative stress by up-regulating miR-190 expression.


Assuntos
Apoptose , Cumarínicos , Células Endoteliais , Glucose , MicroRNAs , Espécies Reativas de Oxigênio , Humanos , MicroRNAs/metabolismo , Apoptose/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/metabolismo , Cumarínicos/farmacologia , Superóxido Dismutase/metabolismo , Malondialdeído/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Vasos Retinianos/efeitos dos fármacos , Vasos Retinianos/metabolismo , Células Cultivadas
7.
J Nanobiotechnology ; 22(1): 256, 2024 May 16.
Artigo em Inglês | MEDLINE | ID: mdl-38755613

RESUMO

BACKGROUND: Gastric cancer represents a highly lethal malignancy with an elevated mortality rate among cancer patients, coupled with a suboptimal postoperative survival prognosis. Nectin-4, an overexpressed oncological target for various cancers, has been exploited to create antibody-drug conjugates (ADCs) to treat solid tumors. However, there is limited research on Nectin-4 ADCs specifically for gastric cancer, and conventional immunoglobulin G (IgG)-based ADCs frequently encounter binding site barriers. Based on the excellent tumor penetration capabilities inherent in nanobodies (Nbs), we developed Nectin-4-targeting Nb drug conjugates (NDCs) for the treatment of gastric cancer. RESULTS: An immunized phage display library was established and employed for the selection of Nectin-4-specific Nbs using phage display technology. Subsequently, these Nbs were engineered into homodimers to enhance Nb affinity. To prolong in vivo half-life and reduce immunogenicity, we fused an Nb targeting human serum albumin (HSA), resulting in the development of trivalent humanized Nbs. Further, we site-specifically conjugated a monomethyl auristatin E (MMAE) at the C-terminus of the trivalent Nbs, creating Nectin-4 NDC (huNb26/Nb26-Nbh-MMAE) with a drug-to-antibody ratio (DAR) of 1. Nectin-4 NDC demonstrated excellent in vitro cell-binding activities and cytotoxic efficacy against cells with high Nectin-4 expression. Subsequent administration of Nectin-4 NDC to mice bearing NCI-N87 human gastric cancer xenografts demonstrated rapid tissue penetration and high tumor uptake through in vivo imaging. Moreover, Nectin-4 NDC exhibited noteworthy dose-dependent anti-tumor efficacy in in vivo studies. CONCLUSION: We have engineered a Nectin-4 NDC with elevated affinity and effective tumor uptake, further establishing its potential as a therapeutic agent for gastric cancer.


Assuntos
Antineoplásicos , Moléculas de Adesão Celular , Imunoconjugados , Camundongos Nus , Anticorpos de Domínio Único , Neoplasias Gástricas , Neoplasias Gástricas/tratamento farmacológico , Humanos , Animais , Anticorpos de Domínio Único/química , Anticorpos de Domínio Único/farmacologia , Moléculas de Adesão Celular/metabolismo , Linhagem Celular Tumoral , Camundongos , Imunoconjugados/química , Imunoconjugados/farmacologia , Imunoconjugados/farmacocinética , Antineoplásicos/farmacologia , Antineoplásicos/química , Camundongos Endogâmicos BALB C , Feminino , Ensaios Antitumorais Modelo de Xenoenxerto , Oligopeptídeos/química , Oligopeptídeos/farmacologia , Nectinas
8.
Crit Rev Biotechnol ; : 1-17, 2024 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-38710624

RESUMO

Constipation is a common gastrointestinal condition, which may occur at any age and affects countless people. The search for new treatments for constipation is ongoing as current drug treatments fail to provide fully satisfactory results. In recent years, probiotics have attracted much attention because of their demonstrated therapeutic efficacy and fewer side effects than pharmaceutical products. Many studies attempted to answer the question of how probiotics can alleviate constipation. It has been shown that different probiotic strains can alleviate constipation by different mechanisms. The mechanisms on probiotics in relieving constipation were associated with various aspects, including regulation of the gut microbiota composition, the level of short-chain fatty acids, aquaporin expression levels, neurotransmitters and hormone levels, inflammation, the intestinal environmental metabolic status, neurotrophic factor levels and the body's antioxidant levels. This paper summarizes the perception of the mechanisms on probiotics in relieving constipation and provides some suggestions on new research directions.

9.
Comput Biol Med ; 173: 108381, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38569237

RESUMO

Multimodal medical image fusion (MMIF) technology plays a crucial role in medical diagnosis and treatment by integrating different images to obtain fusion images with comprehensive information. Deep learning-based fusion methods have demonstrated superior performance, but some of them still encounter challenges such as imbalanced retention of color and texture information and low fusion efficiency. To alleviate the above issues, this paper presents a real-time MMIF method, called a lightweight residual fusion network. First, a feature extraction framework with three branches is designed. Two independent branches are used to fully extract brightness and texture information. The fusion branch enables different modal information to be interactively fused at a shallow level, thereby better retaining brightness and texture information. Furthermore, a lightweight residual unit is designed to replace the conventional residual convolution in the model, thereby improving the fusion efficiency and reducing the overall model size by approximately 5 times. Finally, considering that the high-frequency image decomposed by the wavelet transform contains abundant edge and texture information, an adaptive strategy is proposed for assigning weights to the loss function based on the information content in the high-frequency image. This strategy effectively guides the model toward preserving intricate details. The experimental results on MRI and functional images demonstrate that the proposed method exhibits superior fusion performance and efficiency compared to alternative approaches. The code of LRFNet is available at https://github.com/HeDan-11/LRFNet.


Assuntos
Processamento de Imagem Assistida por Computador , Análise de Ondaletas
10.
Sci Rep ; 14(1): 3473, 2024 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-38347077

RESUMO

High-power inductors are fundamental components in high-power DC-DC converters, with their performance being a crucial metric of converter efficiency. This paper presents an in-depth analysis of a novel calculation method for the air gap length in such inductors. Taking into account the effects of air gap diffusion and the winding magnetic field, an expression for the air gap diffusion radius is derived, focusing on a distributed air gap structure. Furthermore, models for calculating the air gap and winding reluctance are developed, grounded in electromagnetic field theory. An equivalent magnetic circuit model, formulated based on Kirchhoff's second law, facilitates the proposed method for air gap length calculation. This study also involves the development of 3D models for both discrete and decoupled integrated inductors. The comparison between simulation outcomes and calculated air gap lengths indicates a maximum error of less than 8%, with the minimum error being as low as - 0.79%. Compared with traditional methods, the calculation method proposed in this paper has significant advantages. Additionally, the discrepancy between calculated values and experimental measurements is found to be 1.11%. These results validate the accuracy and applicability of the theoretical analysis and calculation method, underscoring their significance in the design and optimization of high-power DC-DC converters.

11.
Environ Sci Pollut Res Int ; 31(10): 15209-15222, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38289558

RESUMO

Utilizing cost-effective corn cob, zinc chloride-modified biochar was synthesized through one-step method for benzene adsorption from air. Study on impregnation ratio impact showed optimal benzene adsorption at ZnCl2:CC ratio of 1.5:1, with capacity reaching 170.53 mg g-1. Characterization using BET, SEM, FTIR, and XPS was conducted. BET results indicated specific surface area of Zn1.5BC at 1260.63 m2 g-1 and maximum pore volume of 0.546 m3 g-1. SEM analysis revealed microporous-mesoporous structure in Zn1.5BC, marking significant improvement over original biomass. DFT pore size distribution and FTIR analysis suggested post-modification dehydration and elimination reactions, leading to volatile compound release, functional group reduction, and pore widening. XPS analysis showed decrease in O = C-OH content with increased impregnation ratio, enhancing biochar's π-π electron diffusion for benzene. Langmuir isotherm and pseudo-second-order kinetic models effectively described experimental data, indicating multilayer benzene adsorption on biochar controlled by complex physicochemical adsorption and pore diffusion. Adsorption condition assessment, including adsorption temperature (20-120 â„ƒ) and benzene concentration in inlet phase (159.73-383.36 mg L-1), was performed. Yoon-Nelson model fitting indicated adsorption site loss at higher temperatures and reduced capture ability due to increased adsorbate molecule kinetic energy. Higher adsorbate concentrations aided adsorption molecule diffusion to biochar surface and internal pores, increasing adsorption rate and shortening equilibrium time. Overall, zinc chloride-modified biochar facilitates benzene adsorption through pore filling and π-π interactions, with pore filling as primary mechanism. Produced biochar shows excellent regeneration properties and reusability.


Assuntos
Benzeno , Cloretos , Poluentes Químicos da Água , Compostos de Zinco , Zea mays , Adsorção , Poluentes Químicos da Água/química , Carvão Vegetal/química , Cinética
12.
Genes Dis ; 11(3): 100986, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38292181

RESUMO

Osteoarthritis and psoriasis arthritis are two degenerative forms of arthritis that share similar yet also different manifestations at the histological, cellular, and clinical levels. Rheumatologists have marked them as two entirely distinct arthropathies. Given recent discoveries in disease initiation and progression, potential mechanisms, cellular signaling pathways, and ongoing clinical therapeutics, there are now more opportunities for discovering osteoarthritis drugs. This review summarized the osteoarthritis and psoriasis arthritis signaling pathways, crosstalk between BMP, WNT, TGF-ß, VEGF, TLR, and FGF signaling pathways, biomarkers, and anatomical pathologies. Through bench research, we demonstrated that regenerative medicine is a promising alternative for treating osteoarthritis by highlighting significant scientific discoveries on entheses, multiple signaling blockers, and novel molecules such as immunoglobulin new antigen receptors targeted for potential drug evaluation. Furthermore, we offered valuable therapeutic approaches with a multidisciplinary strategy to treat patients with osteoarthritis or psoriasis arthritis in the coming future in the clinic.

13.
Small Methods ; 8(3): e2300793, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38009512

RESUMO

The high-efficient and low-cost oxygen evolution reaction (OER) is decisive for applications of oxide catalysts in metal-air batteries, electrolytic cells, and energy-storage technologies. Delicate regulations of active surface and catalytic reaction pathway of oxide materials principally determine thermodynamic energy barrier and kinetic rate during catalytic reactions, and thus have crucial impacts on OER performance. Herein, a synergistic modulation of catalytically active surface and reaction pathway through facile topotactic transformations switching from perovskite (PV) LaNiO3.0 film to infinite-layer (IL) LaNiO2.0 film is demonstrated, which absolutely contributes to improving OER performance. The square-planar NiO4 coordination of IL-LaNiO2.0 brings about more electrochemically active metal (Ni+ ) sites on the film surface. Meanwhile, the oxygen-deficient driven PV- IL topotactic transformations lead to a reaction pathway converted from absorbate evolution mechanism to lattice-oxygen-mediated mechanism (LOM). The non-concerted proton-electron transfer of LOM pathway, evidenced by the pH-dependent OER kinetics, further boosts the OER activity of IL-LaNiO2.0 films. These findings will advance the in-depth understanding of catalytic mechanisms and open new possibilities for developing highly active perovskite-derived oxide catalysts.

14.
J Pediatr Nurs ; 75: 99-107, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38134732

RESUMO

PURPOSE: To explore the lived experiences of the caregivers of children with developmental delay (DD) on the remote island of Kinmen, Taiwan. DESIGN AND METHODS: This descriptive phenomenological study included 14 caregivers of 16 children with DD recruited from Kinmen, Taiwan. Data were collected from September 2020 to February 2021 with face-to-face semi-structured interviews, which were transcribed verbatim and analyzed using thematic analysis. RESULTS: Twelve caregivers were mothers; there was one father and one grandmother. Their mean age was 37.93 ± 5.17 years. The mean age of the children was 3.90 ± 1.49 years; 11 were male (69%). As we began our study, the COVID-19 pandemic occurred, which influenced two of the three themes: (1) focusing on their child's delayed development, which involved seeking a diagnosis and blaming themselves; (2) facing barriers to caring a child with DD on a remote island, which included ostracization of their child and limited off-island medical resources, especially during COVID-19; and (3) maintaining a healthy life balance, which involved protecting their child from physical or spiritual harm, including exposure to COVID-19, and reflecting on their strengths. CONCLUSIONS: Our findings highlight the challenges of caring for a child with DD in the Kinmen islands of Taiwan, and the impact of the COVID-19 pandemic. PRACTICAL IMPLICATIONS: Healthcare professionals are encouraged to provide caregivers of children with DD living in remote, island communities an opportunity to share their caregiving experiences and provide strategies for obtaining medical care, which could help reduce caregiver burden.


Assuntos
COVID-19 , Cuidadores , Criança , Feminino , Humanos , Masculino , Adulto , Pré-Escolar , Estresse Psicológico , Pandemias , Mães , COVID-19/epidemiologia , Pesquisa Qualitativa
15.
Nanomaterials (Basel) ; 13(24)2023 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-38132996

RESUMO

The detection and feedback of displacement and velocity significantly impact the control accuracy of the linear feed system. In this study, we propose a flexible and self-powered displacement sensor based on the triboelectric effect, designed for seamless integration into linear feed systems. The displacement sensor comprises two parts, the mover and stator, operating in a sliding mode. This sensor can precisely detect the displacement of the linear feed system with a large detection range. Additionally, the sensor is capable of real-time velocity detection of linear feed systems, with an error rate below 0.5%. It also offers advantages, such as excellent flexibility, compact size, stability, easy fabrication, and seamless integration, with linear feed systems. These results highlight the potential of the self-powered displacement sensor for various applications in linear feed systems.

16.
Ecotoxicol Environ Saf ; 267: 115666, 2023 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-37944465

RESUMO

BACKGROUND: Exposure to inhalable environmental particulate matter with a diameter of 2.5 µm or smaller (PM2.5) is associated with decreased or impaired kidney function, but the underlying biological mechanisms are not fully understood. Gut microbiota is an emerging key player in the homeostasis regulation of the gut-kidney axis. Few studies have investigated its role in PM2.5 exposure-induced gut-kidney axis homeostasis abnormalities. METHODS: In this study, a versatile aerosol concentration enrichment system for medium- to long-term whole-body exposure was used to expose Sprague-Dawley rats to filtered air (FA) or concentrated ambient PM2.5 for 12 weeks. A correlation analysis of renal impairment and the intestinal microbiome was performed. RESULTS: The urine flow rate calculation and renal function analysis showed that PM2.5 exposure significantly impaired renal function and increased the urine flow rate. The fecal microbiota analysis showed that renal impairment and increased urine flow rates were consistent with the reduced estimates of the fecal bacteria Chao1, observed-species, Shannon, and Simpson (richness and diversity indices). Pearson's correlation analysis showed that the estimated bacterial richness and diversity were correlated with the urine flow rate and renal function. The linear discriminant analysis effect size (LEfSe) analysis revealed differences between animals exposed to PM2.5 and FA in 25 bacterial groups. Further correlation of a single bacterial taxon with the urine flow rate and renal function showed that the relative abundances of 30, 29, 21, and 50 distinct bacterial groups were significantly correlated with the urine flow rate, estimated glomerular filtration rate (eGFR), serum cystatin C (CysC), and beta-2 microglobulin (ß2-MG), respectively. CONCLUSION: Subchronic exposure to PM2.5 can cause intestinal ecological disorders, which may, in turn, lead to decreased kidney function or the development of impaired kidney function.


Assuntos
Poluentes Atmosféricos , Microbioma Gastrointestinal , Ratos , Animais , Ratos Sprague-Dawley , Material Particulado/toxicidade , Material Particulado/análise , Rim/fisiologia , Rim/química , Taxa de Filtração Glomerular , Poluentes Atmosféricos/análise , Exposição Ambiental/análise
17.
J Nanobiotechnology ; 21(1): 410, 2023 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-37932752

RESUMO

BACKGROUND: Pancreatic cancer is a highly aggressive malignancy with limited treatment options and a poor prognosis. Trophoblast cell surface antigen 2 (TROP2), a cell surface antigen overexpressed in the tumors of more than half of pancreatic cancer patients, has been identified as a potential target for antibody-drug conjugates (ADCs). Almost all reported TROP2-targeted ADCs are of the IgG type and have been poorly studied in pancreatic cancer. Here, we aimed to develop a novel nanobody-drug conjugate (NDC) targeting TROP2 for the treatment of pancreatic cancer. RESULTS: In this study, we developed a novel TROP2-targeted NDC, HuNbTROP2-HSA-MMAE, for the treatment of TROP2-positive pancreatic cancer. HuNbTROP2-HSA-MMAE is characterized by the use of nanobodies against TROP2 and human serum albumin (HSA) and has a drug-antibody ratio of 1. HuNbTROP2-HSA-MMAE exhibited specific binding to TROP2 and was internalized into tumor cells with high endocytosis efficiency within 5 h, followed by intracellular translocation to lysosomes and release of MMAE to induce cell apoptosis in TROP2-positive pancreatic cancer cells through the caspase-3/9 pathway. In a xenograft model of pancreatic cancer, doses of 0.2 mg/kg and 1 mg/kg HuNbTROP2-HSA-MMAE demonstrated significant antitumor effects, and a dose of 5 mg/kg even eradicated the tumor. CONCLUSION: HuNbTROP2-HSA-MMAE has desirable affinity, internalization efficiency and antitumor activity. It holds significant promise as a potential therapeutic option for the treatment of TROP2-positive pancreatic cancer.


Assuntos
Imunoconjugados , Neoplasias Pancreáticas , Humanos , Antígenos de Superfície , Linhagem Celular Tumoral , Imunoconjugados/química , Neoplasias Pancreáticas/patologia , Ensaios Antitumorais Modelo de Xenoenxerto , Animais , Neoplasias Pancreáticas
18.
bioRxiv ; 2023 Sep 20.
Artigo em Inglês | MEDLINE | ID: mdl-37786679

RESUMO

Human milk oligosaccharides (HMOs) are a diverse class of carbohydrates that aid in the health and development of infants. The vast health benefits of HMOs have made them a commercial target for microbial production; however, producing the ∼130 structurally diverse HMOs at scale has proven difficult. Here, we produce a vast diversity of HMOs by leveraging the robust carbohydrate anabolism of plants. This diversity includes high value HMOs, such as lacto-N-fucopentaose I, that have not yet been commercially produced using state-of-the-art microbial fermentative processes. HMOs produced in transgenic plants provided strong bifidogenic properties, indicating their ability to serve as a prebiotic supplement. Technoeconomic analyses demonstrate that producing HMOs in plants provides a path to the large-scale production of specific HMOs at lower prices than microbial production platforms. Our work demonstrates the promise in leveraging plants for the cheap and sustainable production of HMOs.

19.
Environ Res ; 238(Pt 2): 117265, 2023 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-37775009

RESUMO

Thermal catalytic degradation of formaldehyde (HCHO) over manganese-based catalysts is garnering significant attention. In this study, both theoretical simulations and experimental methods were employed to elucidate the primary reaction pathways of HCHO on the MnO2(110) surface. Specifically, the effects of doping MnO2 with elements such as Fe, Ce, Ni, Co, and Cu on the HCHO oxidation properties were evaluated. Advanced characterization techniques, including X-ray diffraction (XRD), scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), and X-ray photoelectron spectroscopy (XPS), were employed to discern the physical properties and chemical states of the active components on the catalyst surface. The comprehensive oxidation pathway of HCHO on the MnO2(110) surface includes O2 adsorption and dissociation, HCHO adsorption and dehydrogenation, CO2 desorption, H2O formation and desorption, oxygen vacancy supplementation, and other elementary reactions. The pivotal rate-determining step was identified as the hydrogen migration process, characterized by an energy barrier of 234.19 kJ mol-1. Notably, HCHOO and *CHOO emerged as crucial intermediates during the reaction. Among the doped catalysts, Fe-doped MnO2 outperformed its counterparts doped with Ce, Ni, Co, and Cu. The optimal degradation rate and selectivity were achieved at a molar ratio of Fe: Mn = 0.1. The superior performance of the Fe-doped MnO2 can be ascribed to its large specific surface area, conducive pore structure for HCHO molecular transport, rich surface-adsorbed oxygen species, and a significant presence of oxygen vacancies.


Assuntos
Manganês , Óxidos , Óxidos/química , Compostos de Manganês/química , Oxigênio/química , Catálise , Formaldeído/química
20.
Crit Rev Food Sci Nutr ; : 1-21, 2023 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-37691446

RESUMO

Spectroscopic techniques coupled with chemometric approaches have been widely used for quality evaluation of agricultural and food (agri-food) products due to the nondestructive, simple, fast, and easy characters. However, these techniques face the issues or challenges of relatively weak robustness, generalizability, and applicability in modeling and prediction because they measure the aggregate amount of light interaction with tissues, resulting in the combined effect of absorption and scattering of photons. Optical property measurement could separate absorption from scattering, providing new insights into more reliable prediction performance in quality evaluation, which is attracting increasing attention. In this review, a brief overview of the currently popular measurement techniques, in terms of light transfer principles and data analysis algorithms, is first presented. Then, the emphases are put on the recent advances of these techniques for measuring optical properties of agri-food products since 2000. Corresponding applications on qualitative and quantitative analyses of quality evaluation, as well as light transfer simulations within tissues, were reviewed. Furthermore, the leading groups working on optical property measurement worldwide are highlighted, which is the first summary to the best of our knowledge. Finally, challenges for optical property measurement are discussed, and some viewpoints on future research directions are also given.


HighlightsEmerging techniques for measuring optical properties are briefly introducedQualitative analyses of maturity evaluation and defect detection are reviewedQuantitative analyses of attribute prediction and microstructure estimation are presentedLight transfer simulations based on optical properties are comprehensively discussedLeading groups are summarized for the first time, to the best of our knowledgeChallenges and prospects for optical property measurement are given.

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