Effects of sodium lauryl sulfate and postbiotic toothpaste on oral microecology.

The diversity and delicate balance of the oral microbiome contribute to oral health, with its disruption leading to oral and systemic diseases. Toothpaste includes elements like traditional additives such as sodium lauryl sulfate (SLS) as well as novel postbiotics derived from probiotics, which are commonly employed for maintaining oral hygiene and a healthy oral cavity. However, the response of the oral microbiota to these treatments remains poorly understood. In this study, we systematically investigated the impact of SLS, and toothpaste containing postbiotics (hereafter, postbiotic toothpaste) across three systems: biofilms, animal models, and clinical populations. SLS was found to kill bacteria in both preformed biofilms (mature biofilms) and developing biofilms (immature biofilms), and disturbed the microbial community structure by increasing the number of pathogenic bacteria. SLS also destroyed periodontal tissue, promoted alveolar bone resorption, and enhanced the extent of inflammatory response level. The postbiotic toothpaste favored bacterial homeostasis and the normal development of the two types of biofilms in vitro, and attenuated periodontitis and gingivitis in vivo via modulation of oral microecology. Importantly, the postbiotic toothpaste mitigated the adverse effects of SLS when used in combination, both in vitro and in vivo. Overall, the findings of this study describe the impact of toothpaste components on oral microflora and stress the necessity for obtaining a comprehensive understanding of oral microbial ecology by considering multiple aspects.

The impacts of sodium lauroyl sarcosinate in facial cleanser on facial skin microbiome and lipidome.

BACKGROUND: The human skin microbiome and lipidome are essential for skin homeostasis and barrier function, and have become a focus in both dermatological and cosmetic fields. However, the influence of surfactants commonly used in cosmetic products on the skin resident microbiome and lipidome remains poorly characterized. METHODS: We conducted self-control experiments to systematically study the effects of surfactant (sodium lauroyl sarcosinate [SLS]) on facial skin. Wrinkles, pores, porphyrins, and superficial lipids were examined to evaluate the biophysical state of skin. Quantitative real-time PCR was used to detect the numbers of bacteria and fungi. The diversity and structure of prokaryotic and eukaryotic microbiomes were assessed using 16S rDNA and ITS amplicon sequencing, respectively. Moreover, 22 lipids were identified to evaluate lipidome variations. SPSS software was used for statistical analysis. RESULTS: SLS in facial cleanser did not extensively influence skin biophysical parameters, but caused a decrease in porphyrin. After using the SLS-added facial cleanser for 3 weeks, the alpha diversity of the prokaryotic microbial community decreased significantly, while the eukaryotic microbial community showed a continuous downward trend but no statistically significant. A shift in the structure of prokaryotic microbiome was observed as a result of SLS exposure, mainly reflected by the increase in Acinetobacter, Escherichia-Shigella, Streptococcus, and Ralstonia, while the SLS had little effect on the structure of the eukaryotic microbiome. Furthermore, SLS exposure had a great impact on skin lipidome, mainly manifested by the increase of phosphatidylglycerol (PG) and phosphatidylcholine (PC), and the decrease of ceramides. Spearman's correlations analysis showed that Escherichia-Shigella, Pseudomonas, and Acinetobacter are positively correlated with PG and PC; however, the correlation is not statistically significant. CONCLUSION: In this study, we found the SLS in facial cleanser primarily affected lipidome and the prokaryotic microbiome of facial skin. These findings are useful for reminding us to be vigilant about the ingredients in personal care products, even the common ingredients, and designing effective formulations for repairing ecological balance of skin.

Molecular engineering of locked alkyl aryl carbonyl-based thermally activated delayed fluorescence emitters via a cascade C-H activation process.

While diaryl ketones have drawn tremendous attention for the assembly of carbonyl-based thermally activated delayed fluorescence (TADF) emitters, alkyl aryl ketones are almost ignored. In this work, an efficient rhodium-catalyzed cascade C-H activation process of alkyl aryl ketones with phenylboronic acids has been developed for the concise construction of the α,α-dialkyl/aryl phenanthrone skeleton, which unlocks an opportunity to rapidly assemble a library of structurally nontraditional locked alkyl aryl carbonyl-based TADF emitters. Molecular engineering indicates that the introduction of a donor on the A ring enables the emitters to exhibit better TADF properties than those with a donor on the B ring. 2,6-Bis(9,9-dimethylacridin-10(9H)-yl)-10,10-diphenylphenanthren-9(10H)-one (2,6-DMAC-DPPO) with two donors on the A and B rings gives rise to superior organic light-emitting diode (OLED) performance with maximum external quantum efficiency and power efficiency as high as 32.6% and 123.5 lm W-1, respectively.

[Advance of in vitro oral static biofilm model].

Oral static biofilm model is an important tool for in vitro simulation of oral microecological environment, which has become an important method for studying the pathogenesis of various oral diseases and testing the efficacy of various drugs, oral care products and foods due to its low cost, high throughput, good reliability and easy operation. The establishment of oral static biofilm models allows the selection of different devices, inoculum sources, media, substrates and culture conditions according to the purpose of the study, and the evaluation of biofilm growth by various methods such as measuring biomass, metabolic activity, community structure and performing visualization analysis. This paper summarizes the methodological elements reported in recent years for the establishment and evaluation of oral static biofilm models, and analyzes and discusses the applicability of various methods in the hope of contributing to the research and production practice in related fields.

Bleeding Outcomes After Dental Extraction in Patients Under Direct-Acting Oral Anticoagulants vs. Vitamin K Antagonists: A Systematic Review and Meta-Analysis.

Background: The current systematic review aimed to compare bleeding outcomes in dental extraction patients receiving uninterrupted Direct-acting oral anticoagulant (DOAC) or Vitamin K antagonists (VKAs) for various systemic diseases. Methods: PubMed, Embase, ScienceDirect, CENTRAL, and Google Scholar databases were searched for randomized controlled trials, controlled clinical trials, prospective and retrospective cohort studies, and case control studies, conducted on adult patients undergoing dental extraction under uninterrupted DOAC or VKAs therapy and reporting bleeding outcomes. The search was conducted up to March 31, 2021. We pooled data to calculate risk ratios (RR) with 95% confidence intervals (CI) in a random-effects model. Results: Eight studies comparing 539 patients on DOAC therapy and 574 patients on VKAs were included. Meta-analysis indicated a statistically significant lower bleeding risk in patients under DOAC therapy (RR 0.68 95% CI 0.49, 0.95 I2 = 0%). However, on sensitivity analysis, the results were statistically non-significant after exclusion of any of the included studies. On pooled analysis of limited number of studies, we found no statistically significant difference in the risk of bleeding between apixaban (RR 0.85 95% CI 0.45, 1.60 I2 = 0%), rivaroxaban (RR 0.95 95% CI 0.36, 2.48 I2 = 45%), dabigatran (RR 0.49 95% CI 0.19, 1.28 I2 = 5%), edoxaban (RR 0.41 95% CI 0.13, 1.27 I2 = 0%) and VKAs. Conclusion: The results of the first review comparing bleeding outcomes after dental extraction in patients on uninterrupted DOAC or VKA therapy indicates that patients on DOAC may have a reduced risk of hemorrhage. Current evidence is of very low-quality and should be interpreted with caution. Data on individual DOAC is scarce and at this point, the difference in the risk of bleeding between these drugs cannot be elucidated. Further studies with a large sample size shall supplement our conclusion.