RESUMO
A comparative immunological analysis of the antigenic structure of reference influenza B viruses of 1940-1984 and isolates of 1986-1991 using monospecific antibodies to individual antigenic determinants and monoclonal antibodies to hemagglutinin of B/Oregon/5/80 virus demonstrated a further antigenic drift of influenza B viruses from the reference variants B/Victoria/2/87 and B/Yamagata/16/88. Alongside with circulation of viruses with new antigenic properties, variants were found in the epidemic outbreak of 1991 whose hemagglutinin had common antigenic markers with those of variants of the previous years.
Assuntos
Antígenos Virais/sangue , Vírus da Influenza B/imunologia , Animais , Anticorpos Monoclonais , Variação Antigênica/imunologia , Embrião de Galinha , Ensaio de Imunoadsorção Enzimática , Epitopos/sangue , Testes de Inibição da Hemaglutinação , Hemaglutininas Virais/sangue , Humanos , Vírus da Influenza B/isolamento & purificaçãoRESUMO
Epidemics of influenza in towns of Russia were found to have been caused by influenza type B virus. Strains similar to B/Yamagata/16/88 were first isolated in the country. The study of antigenic and biological properties showed that the epidemic strains could be divided into 2 groups: similar to B/Victoria/2/87 and to B/Yamagata/16/88, that is, representing two branches of the evolutionary tree. In both groups the viruses were heterogeneous both in antigenic and biological properties.
Assuntos
Surtos de Doenças , Vírus da Influenza B/isolamento & purificação , Influenza Humana/microbiologia , Antígenos Virais/análise , Antígenos Virais/classificação , Surtos de Doenças/estatística & dados numéricos , Humanos , Vírus da Influenza B/classificação , Vírus da Influenza B/imunologia , Influenza Humana/epidemiologia , U.R.S.S./epidemiologia , População Urbana/estatística & dados numéricosRESUMO
A comparative study of influenza A (H1N1) virus strains isolated in one epidemic season (1988-1989) in neighbouring countries was carried out. Using polyclonal and monoclonal antibodies, some of the isolates were shown to have different antigenic and biological properties which probably determined the different intensity of the epidemic situation in the USSR and CzSFR in 1988-1989.
Assuntos
Antígenos Virais/sangue , Vírus da Influenza A Subtipo H1N1 , Vírus da Influenza A/imunologia , Tchecoslováquia , Surtos de Doenças , Hemaglutininas Virais/imunologia , Humanos , Vírus da Influenza A/enzimologia , Vírus da Influenza A/isolamento & purificação , Influenza Humana/imunologia , Influenza Humana/microbiologia , Neuraminidase/imunologia , Nucleoproteínas/imunologia , U.R.S.S. , Proteínas Virais/imunologiaRESUMO
Investigations of the antigenic structure and genome of influenza A (H1N1) viruses isolated in the Mongolian People's Republic in 1982, 1983, 1986 and 1987 from children with acute respiratory diseases using monoclonal antibodies and nucleotide sequencing revealed 4 strains identical to the prototype strain A/PR/8/34 (H1N1), variant M. Sinai, and 8 strains closely similar to the epidemic strain A/USSR/90/77 (H1N1) in the antigenic structure and A/Leningrad/54 (H1N1) in the primary structure of HA.
Assuntos
Vírus da Influenza A Subtipo H1N1 , Vírus da Influenza A/imunologia , Antígenos Virais/análise , Sequência de Bases , Criança , Pré-Escolar , Epitopos/análise , Testes de Inibição da Hemaglutinação , Humanos , Lactente , Vírus da Influenza A/enzimologia , Vírus da Influenza A/genética , Vírus da Influenza A/isolamento & purificação , Influenza Humana/microbiologia , Dados de Sequência Molecular , Mongólia , Neuraminidase/análise , Neuraminidase/genéticaRESUMO
Monoclonal antibodies, epidemic strains of influenza A and B viruses, conjugates were studied by fluorescence immunoassay with temporary resolution using equipment of different companies. Differences and specificity of monoclonal antibodies to influenza A and B viruses were demonstrated. The highest sensitivity of the method with the test system used was determined, being 20 ng/ml of viral protein. The method was shown to be useful for influenza virus detection both in solutions containing purified virions and in virus-containing allantoic preparations. It was established that virological studies could be carried out using conjugates and knock-down microplates of the national make alongside with foreign ones.
Assuntos
Vírus da Influenza A/imunologia , Vírus da Influenza B/imunologia , Anticorpos Monoclonais , Anticorpos Antivirais/análise , Especificidade de Anticorpos/imunologia , Estudos de Avaliação como Assunto , Imunofluorescência/instrumentação , Proteínas do Core Viral/imunologiaRESUMO
A comparative study of antigenic and molecular-biological properties of epidemic influenza virus strains isolated in the USSR and CSSR in the autumn-winter, 1986-1987 was carried out and showed that the epidemics in both countries were due to influenza A viruses of H1N1 subtype, antigenically related to the reference A/Taiwan/1/86 and A/Singapore/6/86 strains, which was confirmed by electrophoretic studies. The strains were found to vary in sensitivity to heating and to the inhibitors.
Assuntos
Influenza Humana/epidemiologia , Tchecoslováquia , Eletroforese em Gel de Poliacrilamida , Hemaglutininas Virais/análise , Temperatura Alta , Humanos , Vírus da Influenza A Subtipo H1N1 , Vírus da Influenza A/efeitos dos fármacos , Vírus da Influenza A/imunologia , Vírus da Influenza A/isolamento & purificação , Influenza Humana/imunologia , Neuraminidase/análise , U.R.S.S.RESUMO
The primary structure of hemagglutinin of the members of three main antigenic groups of current influenza A (H3N2) viruses was determined. A phenomenon of "antigenic mimicry" was found to be due to the accumulation of reversions in hemagglutinin structure at late stages of antigenic drift.
Assuntos
Hemaglutininas Virais/genética , Vírus da Influenza A Subtipo H3N2 , Vírus da Influenza A/genética , Sequência de Aminoácidos , Variação Antigênica , Sequência de Bases , Genes Virais , Glicoproteínas de Hemaglutininação de Vírus da Influenza , Hemaglutininas Virais/imunologia , Humanos , Vírus da Influenza A/imunologia , Vírus da Influenza A/isolamento & purificação , Modelos Moleculares , Dados de Sequência Molecular , Mutação , U.R.S.S.RESUMO
The study included 230 strains of influenza A (H3N2) virus isolated in the epidemic of 1985. A high degree of heterogeneity of the virus population was established with polyclonal sera, monoclonal antibodies and the method of kRNA-bRNA hybridization for the determination of the genome composition. Among the strains of one epidemic (1985) three antigenically heterogenous groups of strains were detected similar with reference A/Philippines/2/82, A/Ken/1/84, and A/Mississippi/1/85 strains. It was shown that in the process of antigenic drift in some strains there occurred a reversion of the antigenic determinants similar to those of previously circulating epidemic A/Texas/1/77 strain.
Assuntos
Variação Antigênica , Antígenos Virais/imunologia , Vírus da Influenza A Subtipo H3N2 , Vírus da Influenza A/imunologia , Infecções por Orthomyxoviridae/microbiologia , Animais , Anticorpos Monoclonais , Surtos de Doenças , Eletroforese em Gel de Poliacrilamida , Epitopos/imunologia , Hemaglutininas Virais/imunologia , Hibridização de Ácido Nucleico , Infecções por Orthomyxoviridae/epidemiologia , RatosRESUMO
Influenza A viruses (H3N2) isolated in the USSR, 1986, from children were antigenically similar to the prototype A/Hong Kong/1/68 strain. Alongside with the similarity with the latter virus, the new isolates had a number of distinctive features both in the antigenic specificity of hemagglutinin and in the structure of the polymerase complex proteins. All the foregoing allows to exclude laboratory contamination and suggests the possibility of circulation of such viruses among children.
Assuntos
Antígenos Virais/análise , Vírus da Influenza A Subtipo H3N2 , Vírus da Influenza A/imunologia , Anticorpos Monoclonais , Criança , Surtos de Doenças , Eletroforese em Gel de Poliacrilamida , Epitopos/análise , Hemaglutininas Virais/análise , Humanos , Técnicas Imunológicas , Vírus da Influenza A/enzimologia , Vírus da Influenza A/isolamento & purificação , Influenza Humana/epidemiologia , Influenza Humana/microbiologia , Neuraminidase/análise , Neuraminidase/imunologia , U.R.S.S.RESUMO
The antigenic structure of hemagglutinin of influenza A virus (H3N2) strains isolated in 1985 was studied using a series of monoclonal antibody to A/Dunedin/4/73/A (H3N2) and A/Bangkok/1/79/A (H3N2), and biological and physico-chemical properties of these strains were compared with those of influenza A (H3N2) virus of 1983 and reference A (H3N2) of 1979-1984 (the rate of adsorption on chick erythrocytes and eluting activity, thermostability of hemagglutinin and neuraminidase, sensitivity to nonionic detergents, sensitivity to remantadine, analysis of virion polypeptide composition). A high degree of heterogeneity of the 1985 strain population of influenza A (H3N2) virus was revealed both in the antigenic structure of hemagglutinin and in all the biological and physico-chemical parameters tested. It was suggested that the A/Caen/1/84 strain originated not from A/Philippines/2/82 but directly from A/Texas/1/77.
Assuntos
Antígenos Virais/análise , Vírus da Influenza A/imunologia , Adsorção , Anticorpos Monoclonais/análise , Fenômenos Químicos , Físico-Química , Eletroforese em Gel de Poliacrilamida , Testes de Inibição da Hemaglutinação , Hemaglutininas Virais/análise , Humanos , Vírus da Influenza A/análise , Vírus da Influenza A/efeitos dos fármacos , Vírus da Influenza A/isolamento & purificação , Rimantadina/farmacologia , Proteínas Virais/análiseRESUMO
The antigenic properties of neuraminidase of the epidemic strains of 1983-1985 were studied by means of lectin test and thiobarbituric method using polyclonal sera and monoclonal antibody. Populations of the viruses with respect to antigenic relationships of neuraminidase were shown to be heterogeneous. A considerable portion of the strains occurring in 1985 underwent antigenic drift from the reference A/Philippines/2/82 strain. The epidemic novelty of some strains was due to changes in only one supercapsid protein-neuraminidase.
Assuntos
Antígenos Virais/análise , Epitopos/análise , Vírus da Influenza A Subtipo H3N2 , Vírus da Influenza A/imunologia , Neuraminidase/imunologia , Anticorpos Monoclonais/análise , Variação Antigênica , Surtos de Doenças , Humanos , Técnicas Imunológicas , Vírus da Influenza A/enzimologia , Vírus da Influenza A/isolamento & purificação , Influenza Humana/microbiologia , U.R.S.S.RESUMO
The antigenic structure of neuraminidases of influenza B viruses isolated in 1940-1984 was studied. Lectin test has first been employed for analysis of influenza B virus neuraminidase. The antigenic composition of neuraminidases of these viruses was shown to be different. The strains isolated in the epidemic of 1983-1984 underwent further drift of the neuraminidase component and represent a heterogeneous group in this respect. The use of monospecific antiserum to purified neuraminidase helped specify antigenic relationships of this protein.
Assuntos
Antígenos Virais/análise , Epitopos/análise , Vírus da Influenza B/imunologia , Neuraminidase/imunologia , Surtos de Doenças , Humanos , Técnicas Imunológicas , Vírus da Influenza B/enzimologia , Vírus da Influenza B/isolamento & purificação , Influenza Humana/microbiologia , U.R.S.S.RESUMO
A method for isolation of neuraminidase using pronase has been developed. Purified preparations have been recovered from two influenza B virus strains, B/Mass/3/66 and B/USSR/14/80. Physico-chemical properties of neuraminidases were compared; optimal conditions for the determination of neuraminidase activity of these strains were determined. Strains differences in thermostability, optimal pH, specificity to substrates were established. Neuraminidases, isolated or present in virions, were shown to be identical in enzymatic characteristics but to differ in thermostability.
Assuntos
Vírus da Influenza B/enzimologia , Neuraminidase/análise , Vírion/enzimologia , Fenômenos Químicos , Físico-Química , Concentração de Íons de Hidrogênio , Cinética , Neuraminidase/isolamento & purificação , Neuraminidase/metabolismo , Pronase/metabolismo , Especificidade por Substrato , TemperaturaAssuntos
Capsídeo/biossíntese , Infecções por Orthomyxoviridae/metabolismo , Proteínas do Core Viral/biossíntese , Animais , Capsídeo/análise , Células Cultivadas , Embrião de Galinha , Fibroblastos/metabolismo , Fibroblastos/microbiologia , Vírus da Influenza A , Vírus da Influenza B , Gammainfluenzavirus , Infecções por Orthomyxoviridae/microbiologia , Fenótipo , Proteínas do Core Viral/análiseRESUMO
Analysis of comparative surveillance on influenza carried out in the USSR and the GDR is presented. It was shown that both in the nonepidemic and epidemic seasons the incidence of influenza in the USSR increased considerably earlier than in the GDR. In the nonepidemic season of 1978-1979, strains of different antigenic structure were in circulation in the USSR and the GDR, whereas the epidemic of 1979-1980 was induced by new drift variants of A(H3N2) virus, A/Bangkok/1/79 and A/Bangkok/2/79. The epidemic strains circulating in both countries were dissimilar biologically and antigenically. In the nonepidemic period in the USSR and GDR the circulating A(H1N1) strains were represented not only by drift variants A/USSR/90/77 and A/Brazil/11/78 but also by natural recombinants of A(H1N1), A/USSR/61/79, in which the internal proteins (P, NP, NS, and M) were analogous to those of A(H3N2).
Assuntos
Surtos de Doenças/epidemiologia , Influenza Humana/epidemiologia , Estações do Ano , Antígenos Virais/análise , Variação Genética , Alemanha Oriental , Hemaglutininas Virais/análise , Humanos , Vírus da Influenza A/imunologia , Influenza Humana/imunologia , U.R.S.S.RESUMO
Antigenic relationships of the vaccine A/Victoria/35/72 strain and vaccine candidate A/Khabarovsk/15/76 and recombinant RK-5 strains with hemagglutinins of the epidemic A/Victoria/3/75, A/Leningrad/173/75, and A/Victoria/112/76 strains were studied by radioimmunoassay (RIA) in two test systems. Alongside with other tests RIA is useful and expedient for rapid and accurate quantitative evaluation of the antigenic conformity of influenza virus strains under comparison.
Assuntos
Antígenos Virais/análise , Hemaglutininas Virais/análise , Vírus da Influenza A/imunologia , Vacinas contra Influenza/imunologia , Ligação Competitiva , Radioimunoensaio/métodosRESUMO
Heterogeneity of antibodies against influenza virus hemagglutinin has been confirmed. Various subpopulations of antibodies to the A/Khabarovsk/15/76 hemagglutinin were demonstrated some of which showed greater affinity to MRC-11 hemagglutinin than to the HA of the strain inducing this antibody synthesis. Apparently subpopulations of antibodies with low affinity to their "own" epitopes in the hemagglutinin-immunogen may develop which, however, may show a sufficiently high affinity to these epitopes in hemagglutinins of other strains.