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1.
Harmful Algae ; 133: 102599, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38485445

RESUMO

Cyanobacterial blooms present substantial challenges to managers and threaten ecological and public health. Although the majority of cyanobacterial bloom research and management focuses on factors that control bloom initiation, duration, toxicity, and geographical extent, relatively little research focuses on the role of loss processes in blooms and how these processes are regulated. Here, we define a loss process in terms of population dynamics as any process that removes cells from a population, thereby decelerating or reducing the development and extent of blooms. We review abiotic (e.g., hydraulic flushing and oxidative stress/UV light) and biotic factors (e.g., allelopathic compounds, infections, grazing, and resting cells/programmed cell death) known to govern bloom loss. We found that the dominant loss processes depend on several system specific factors including cyanobacterial genera-specific traits, in situ physicochemical conditions, and the microbial, phytoplankton, and consumer community composition. We also address loss processes in the context of bloom management and discuss perspectives and challenges in predicting how a changing climate may directly and indirectly affect loss processes on blooms. A deeper understanding of bloom loss processes and their underlying mechanisms may help to mitigate the negative consequences of cyanobacterial blooms and improve current management strategies.


Assuntos
Cianobactérias , Proliferação Nociva de Algas , Cianobactérias/fisiologia
2.
FEMS Microbiol Rev ; 46(6)2022 11 02.
Artigo em Inglês | MEDLINE | ID: mdl-35749580

RESUMO

David Schindler and his colleagues pioneered studies in the 1970s on the role of phosphorus in stimulating cyanobacterial blooms in North American lakes. Our understanding of the nuances of phosphorus utilization by cyanobacteria has evolved since that time. We review the phosphorus utilization strategies used by cyanobacteria, such as use of organic forms, alternation between passive and active uptake, and luxury storage. While many aspects of physiological responses to phosphorus of cyanobacteria have been measured, our understanding of the critical processes that drive species diversity, adaptation and competition remains limited. We identify persistent critical knowledge gaps, particularly on the adaptation of cyanobacteria to low nutrient concentrations. We propose that traditional discipline-specific studies be adapted and expanded to encompass innovative new methodologies and take advantage of interdisciplinary opportunities among physiologists, molecular biologists, and modellers, to advance our understanding and prediction of toxic cyanobacteria, and ultimately to mitigate the occurrence of blooms.


Assuntos
Cianobactérias , Lagos , Lagos/microbiologia , Eutrofização , Cianobactérias/fisiologia , Fósforo , Nitrogênio
3.
ISME Commun ; 2(1): 93, 2022 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-37938757

RESUMO

Chytrids are important drivers of aquatic ecosystems as phytoplankton parasites. The interaction between these parasites and their hosts are shaped by abiotic factors such as temperature and light. Here, we performed a full-factorial experiment to study how temperature and light interact to affect the dynamics of the bloom-forming toxic cyanobacterium Planktothrix rubescens and its chytrid parasite. We used a dynamic host-parasite model to explore how temperature and light affect long term dynamics. At low temperatures, chytrids do not survive. Higher light and temperature levels stimulated both phytoplankton and chytrid growth, with complex effects on their dynamics. Model exploration indicates that increasing temperature and light shifts equilibrium outcomes from P. rubescens persisting alone to stable coexistence and then to limit cycles. This provides an alternative biological explanation for why P. rubescens is mainly found in the relatively cold and dark lake metalimnion - it may enable avoidance of its parasite. Our study emphasizes the importance of investigating how abiotic factors interact with biotic interactions to drive complex outcomes.

4.
Glob Chang Biol ; 26(5): 2756-2784, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32133744

RESUMO

In many regions across the globe, extreme weather events such as storms have increased in frequency, intensity, and duration due to climate change. Ecological theory predicts that such extreme events should have large impacts on ecosystem structure and function. High winds and precipitation associated with storms can affect lakes via short-term runoff events from watersheds and physical mixing of the water column. In addition, lakes connected to rivers and streams will also experience flushing due to high flow rates. Although we have a well-developed understanding of how wind and precipitation events can alter lake physical processes and some aspects of biogeochemical cycling, our mechanistic understanding of the emergent responses of phytoplankton communities is poor. Here we provide a comprehensive synthesis that identifies how storms interact with lake and watershed attributes and their antecedent conditions to generate changes in lake physical and chemical environments. Such changes can restructure phytoplankton communities and their dynamics, as well as result in altered ecological function (e.g., carbon, nutrient and energy cycling) in the short- and long-term. We summarize the current understanding of storm-induced phytoplankton dynamics, identify knowledge gaps with a systematic review of the literature, and suggest future research directions across a gradient of lake types and environmental conditions.


Assuntos
Lagos , Fitoplâncton , Mudança Climática , Ecossistema , Rios
5.
ISME J ; 13(5): 1133-1143, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30607028

RESUMO

Here we aim to incorporate trait-based information into the modern coexistence framework that comprises a balance between stabilizing (niche-based) and equalizing (fitness) mechanisms among interacting species. Taking the modern coexistence framework as our basis, we experimentally tested the effect of size differences among species on coexistence by using fifteen unique pairs of resident vs. invading cyanobacteria, resulting in thirty unique invasibility tests. The cyanobacteria covered two orders of magnitude differences in size. We found that both niche and fitness differences increased with size differences. Niche differences increased faster with size differences than relative fitness differences and whereas coexisting pairs showed larger size differences than non-coexisting pairs, ultimately species coexistence could not be predicted on basis of size differences only. Our findings suggest that size is more than a key trait controlling physiological and population-level aspects of phytoplankton, it is also relevant for community-level phenomena such as niche and fitness differences which influence coexistence and biodiversity.


Assuntos
Cianobactérias/fisiologia , Fitoplâncton/fisiologia , Biodiversidade , Cianobactérias/citologia , Ecossistema , Aptidão Genética , Fenótipo , Fitoplâncton/citologia
6.
Nat Rev Microbiol ; 16(8): 471-483, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29946124

RESUMO

Cyanobacteria can form dense and sometimes toxic blooms in freshwater and marine environments, which threaten ecosystem functioning and degrade water quality for recreation, drinking water, fisheries and human health. Here, we review evidence indicating that cyanobacterial blooms are increasing in frequency, magnitude and duration globally. We highlight species traits and environmental conditions that enable cyanobacteria to thrive and explain why eutrophication and climate change catalyse the global expansion of cyanobacterial blooms. Finally, we discuss management strategies, including nutrient load reductions, changes in hydrodynamics and chemical and biological controls, that can help to prevent or mitigate the proliferation of cyanobacterial blooms.


Assuntos
Mudança Climática , Cianobactérias/fisiologia , Ecossistema , Eutrofização , Água Doce/microbiologia , Água do Mar/microbiologia
7.
Environ Microbiol ; 19(10): 3802-3822, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-28618196

RESUMO

Chytridiomycota, often referred to as chytrids, can be virulent parasites with the potential to inflict mass mortalities on hosts, causing e.g. changes in phytoplankton size distributions and succession, and the delay or suppression of bloom events. Molecular environmental surveys have revealed an unexpectedly large diversity of chytrids across a wide range of aquatic ecosystems worldwide. As a result, scientific interest towards fungal parasites of phytoplankton has been gaining momentum in the past few years. Yet, we still know little about the ecology of chytrids, their life cycles, phylogeny, host specificity and range. Information on the contribution of chytrids to trophic interactions, as well as co-evolutionary feedbacks of fungal parasitism on host populations is also limited. This paper synthesizes ideas stressing the multifaceted biological relevance of phytoplankton chytridiomycosis, resulting from discussions among an international team of chytrid researchers. It presents our view on the most pressing research needs for promoting the integration of chytrid fungi into aquatic ecology.


Assuntos
Quitridiomicetos/classificação , Quitridiomicetos/patogenicidade , Micoses/microbiologia , Fitoplâncton/microbiologia , Animais , Evolução Biológica , Ecologia , Ecossistema , Microbiologia Ambiental , Cadeia Alimentar , Especificidade de Hospedeiro , Filogenia
8.
Environ Sci Technol ; 50(20): 10780-10794, 2016 10 18.
Artigo em Inglês | MEDLINE | ID: mdl-27597444

RESUMO

Recent technological developments have increased the number of variables being monitored in lakes and reservoirs using automatic high frequency monitoring (AHFM). However, design of AHFM systems and posterior data handling and interpretation are currently being developed on a site-by-site and issue-by-issue basis with minimal standardization of protocols or knowledge sharing. As a result, many deployments become short-lived or underutilized, and many new scientific developments that are potentially useful for water management and environmental legislation remain underexplored. This Critical Review bridges scientific uses of AHFM with their applications by providing an overview of the current AHFM capabilities, together with examples of successful applications. We review the use of AHFM for maximizing the provision of ecosystem services supplied by lakes and reservoirs (consumptive and non consumptive uses, food production, and recreation), and for reporting lake status in the EU Water Framework Directive. We also highlight critical issues to enhance the application of AHFM, and suggest the establishment of appropriate networks to facilitate knowledge sharing and technological transfer between potential users. Finally, we give advice on how modern sensor technology can successfully be applied on a larger scale to the management of lakes and reservoirs and maximize the ecosystem services they provide.


Assuntos
Ecossistema , Lagos , Monitoramento Ambiental , Recreação
9.
Harmful Algae ; 49: 63-74, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26435706

RESUMO

Toxic cyanobacteria became more widely recognized as a potential health hazard in the 1990s, and in 1998 the World Health Organization (WHO) first published a provisional Guideline Value of 1 µg L-1 for microcystin-LR in drinking-water. In this publication we compare risk assessment and risk management of toxic cyanobacteria in 17 countries across all five continents. We focus on the three main (oral) exposure vehicles to cyanotoxins: drinking-water, water related recreational and freshwater seafood. Most countries have implemented the provisional WHO Guideline Value, some as legally binding standard, to ensure the distribution of safe drinking-water with respect to microcystins. Regulation, however, also needs to address the possible presence of a wide range of other cyanotoxins and bioactive compounds, for which no guideline values can be derived due to insufficient toxicological data. The presence of microcystins (commonly expressed as microcystin-LR equivalents) may be used as proxy for overall guidance on risk management, but this simplification may miss certain risks, for instance from dissolved fractions of cylindrospermopsin and cyanobacterial neurotoxins. An alternative approach, often taken for risk assessment and management in recreational waters, is to regulate cyanobacterial presence - as cell numbers or biomass - rather than individual toxins. Here, many countries have implemented a two or three tier alert level system with incremental severity. These systems define the levels where responses are switched from Surveillance to Alert and finally to Action Mode and they specify the short-term actions that follow. Surface bloom formation is commonly judged to be a significant risk because of the elevated concentration of microcystins in a scum. Countries have based their derivations of legally binding standards, guideline values, maximally allowed concentrations (or limits named otherwise) on very similar scientific methodology, but underlying assumptions such as bloom duration, average body size and the amount of water consumed while swimming vary according to local circumstances. Furthermore, for toxins with incomplete toxicological data elements of expert judgment become more relevant and this also leads to a larger degree of variation between countries' thresholds triggering certain actions. Cyanobacterial blooms and their cyanotoxin content are a highly variable phenomenon, largely depending on local conditions, and likely concentrations can be assessed and managed best if the specific conditions of the locality are known and their impact on bloom occurrence are understood. Risk Management Frameworks, such as for example the Water Safety Plan concept of the WHO and the 'bathing water profile' of the European Union are suggested to be effective approaches for preventing human exposure by managing toxic cyanobacteria from catchment to consumer for drinking water and at recreational sites.

10.
Microb Ecol ; 68(3): 645-56, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-24863129

RESUMO

Parasite environments are heterogeneous at different levels. The first level of variability is the host itself. The second level represents the external environment for the hosts, to which parasites may be exposed during part of their life cycle. Both levels are expected to affect parasite fitness traits. We disentangle the main and interaction effects of variation in the immediate host environment, here the diatom Asterionella formosa (variables host cell volume and host condition through herbicide pre-exposure) and variation in the external environment (variables host density and acute herbicide exposure) on three fitness traits (infection success, development time and reproductive output) of a chytrid parasite. Herbicide exposure only decreased infection success in a low host density environment. This result reinforces the hypothesis that chytrid zoospores use photosynthesis-dependent chemical cues to locate its host. At high host densities, chemotaxis becomes less relevant due to increasing chance contact rates between host and parasite, thereby following the mass-action principle in epidemiology. Theoretical support for this finding is provided by an agent-based simulation model. The immediate host environment (cell volume) substantially affected parasite reproductive output and also interacted with the external herbicide exposed environment. On the contrary, changes in the immediate host environment through herbicide pre-exposure did not increase infection success, though it had subtle effects on zoospore development time and reproductive output. This study shows that both immediate host and external environment as well as their interaction have significant effects on parasite fitness. Disentangling these effects improves our understanding of the processes underlying parasite spread and disease dynamics.


Assuntos
Quitridiomicetos/fisiologia , Diatomáceas/parasitologia , Meio Ambiente , Interações Hospedeiro-Parasita , Quitridiomicetos/genética , Aptidão Genética , Herbicidas
11.
PLoS One ; 8(8): e71677, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23951218

RESUMO

In ecology and evolution, the primary challenge in understanding the processes that shape biodiversity is to assess the relationship between the phenotypic traits of organisms and the environment. Here we tested for selection on physio-morphological traits measured by scanning flow-cytometry at the individual level in phytoplankton communities under a temporally changing biotic and abiotic environment. Our aim was to study how high-frequency temporal changes in the environment influence biodiversity dynamics in a natural community. We focused on a spring bloom in Lake Zurich (Switzerland), characterized by rapid changes in phytoplankton, water conditions, nutrients and grazing (mainly mediated by herbivore ciliates). We described bloom dynamics in terms of taxonomic and trait-based diversity and found that diversity dynamics of trait-based groups were more pronounced than those of identified phytoplankton taxa. We characterized the linkage between measured phytoplankton traits, abiotic environmental factors and abundance of the main grazers and observed weak but significant correlations between changing abiotic and biotic conditions and measured size-related and fluorescence-related traits. We tested for deviations in observed community-wide distributions of focal traits from random patterns and found evidence for both clustering and even spacing of traits, occurring sporadically over the time series. Patterns were consistent with environmental filtering and phenotypic divergence under herbivore pressure, respectively. Size-related traits showed significant even spacing during the peak of herbivore abundance, suggesting that morphology-related traits were under selection from grazing. Pigment distribution within cells and colonies appeared instead to be associated with acclimation to temperature and water chemistry. We found support for trade-offs among grazing resistance and environmental tolerance traits, as well as for substantial periods of dynamics in which our measured traits were not under selection.


Assuntos
Meio Ambiente , Eutrofização , Fitoplâncton/crescimento & desenvolvimento , Fitoplâncton/genética , Característica Quantitativa Herdável , Estações do Ano , Ecossistema , Citometria de Fluxo , Interação Gene-Ambiente , Lagos , Suíça
12.
PLoS One ; 8(8): e71737, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23990982

RESUMO

The cost of parasitism often depends on environmental conditions and host identity. Therefore, variation in the biotic and abiotic environment can have repercussions on both, species-level host-parasite interaction patterns but also on host genotype-specific susceptibility to disease. We exposed seven genetically different but concurrent strains of the diatom Asterionella formosa to one genotype of its naturally co-occurring chytrid parasite Zygorhizidium planktonicum across five environmentally relevant temperatures. We found that the thermal tolerance range of the tested parasite genotype was narrower than that of its host, providing the host with a "cold" and "hot" thermal refuge of very low or no infection. Susceptibility to disease was host genotype-specific and varied with temperature level so that no genotype was most or least resistant across all temperatures. This suggests a role of thermal variation in the maintenance of diversity in disease related traits in this phytoplankton host. The duration and intensity of chytrid parasite pressure on host populations is likely to be affected by the projected changes in temperature patterns due to climate warming both through altering temperature dependent disease susceptibility of the host and, potentially, through en- or disabling thermal host refugia. This, in turn may affect the selective strength of the parasite on the genetic architecture of the host population.


Assuntos
Quitridiomicetos/fisiologia , Diatomáceas/microbiologia , Temperatura , Adaptação Fisiológica/genética , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados , Análise de Variância , Quitridiomicetos/genética , Diatomáceas/genética , Variação Genética , Genótipo , Interações Hospedeiro-Patógeno/genética , Simbiose/genética
13.
Environ Microbiol ; 15(3): 837-47, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23046213

RESUMO

Parasites play an important role in the regulation of host population growth. How these ubiquitous stressors interact with anthropogenic stressors is less often studied. In a full factorial experiment we explored the independent and combined effects of the widely used herbicide diuron and a chytrid parasite on the fitness of genetically different monoclonal diatom populations. Furthermore, we evaluated how herbicide exposure influenced infection dynamics, parasite fitness and the impact of infectious disease on host populations. We found no evidence of host genetic variation for diuron sensitivity and parasite resistance. Instead, host population phenotype was a decisive factor in controlling parasite growth. Although herbicide exposure initially posed a constraint on disease transmission, it enhanced the spread of disease over time. Consequently, the nature of the parasite-toxicant stressor interaction shifted from antagonistic (on exponential host growth) towards additive (on final uninfected host density). We conclude that herbicide exposure can modify infection dynamics and impact of disease on host populations through the complex interplay between host and parasite growth dynamics and host population phenotype.


Assuntos
Quitridiomicetos/efeitos dos fármacos , Quitridiomicetos/fisiologia , Diatomáceas/efeitos dos fármacos , Diatomáceas/parasitologia , Diurona/toxicidade , Herbicidas/toxicidade , Interações Hospedeiro-Parasita/efeitos dos fármacos
14.
Water Res ; 46(5): 1394-407, 2012 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-22217430

RESUMO

Climate change scenarios predict that rivers, lakes, and reservoirs will experience increased temperatures, more intense and longer periods of thermal stratification, modified hydrology, and altered nutrient loading. These environmental drivers will have substantial effects on freshwater phytoplankton species composition and biomass, potentially favouring cyanobacteria over other phytoplankton. In this Review, we examine how several cyanobacterial eco-physiological traits, specifically, the ability to grow in warmer temperatures; buoyancy; high affinity for, and ability to store, phosphorus; nitrogen-fixation; akinete production; and efficient light harvesting, vary amongst cyanobacteria genera and may enable them to dominate in future climate scenarios. We predict that spatial variation in climate change will interact with physiological variation in cyanobacteria to create differences in the dominant cyanobacterial taxa among regions. Finally, we suggest that physiological traits specific to different cyanobacterial taxa may favour certain taxa over others in different regions, but overall, cyanobacteria as a group are likely to increase in most regions in the future.


Assuntos
Cianobactérias/fisiologia , Água Doce/microbiologia , Biomassa , Mudança Climática , Cianobactérias/genética , Cianobactérias/crescimento & desenvolvimento , Ecossistema , Eutrofização , Água Doce/química , Fixação de Nitrogênio , Fósforo/metabolismo , Fotossíntese , Temperatura
15.
J Phycol ; 48(5): 1197-208, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27011279

RESUMO

Marine and freshwater phytoplankton populations often show large clonal diversity, which is in disagreement with clonal selection of the most vigorous genotype(s). Temporal fluctuation in selection pressures in variable environments is a leading explanation for maintenance of such genetic diversity. To test the influence of temperature as a selection force in continually (seasonally) changing aquatic systems we carried out reaction norms experiments on co-occurring clonal genotypes of a ubiquitous diatom species, Asterionella formosa Hassall, across an environmentally relevant range of temperatures. We report within population genetic diversity and extensive diversity in genotype-specific reaction norms in growth rates and cell size traits. Our results showed genotype by environment interactions, indicating that no genotype could outgrow all others across all temperature environments. Subsequently, we constructed a model to simulate the relative proportion of each genotype in a hypothetical population based on genotype and temperature-specific population growth rates. This model was run with different seasonal temperature patterns. Our modeling exercise showed a succession of two to several genotypes becoming numerically dominant depending on the underlying temperature pattern. The results suggest that (temperature) context dependent fitness may contribute to the maintenance of genetic diversity in isolated populations of clonally reproducing microorganisms in temporally variable environments.

16.
Environ Sci Technol ; 45(22): 9658-65, 2011 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-21981777

RESUMO

High quality monitoring data are vital for tracking and understanding the causes of ecosystem change. We present a potentially powerful approach for phytoplankton and aquatic ecosystem monitoring, based on integration of scanning flow-cytometry for the characterization and counting of algal cells with multiparametric vertical water profiling. This approach affords high-frequency data on phytoplankton abundance, functional traits and diversity, coupled with the characterization of environmental conditions for growth over the vertical structure of a deep water body. Data from a pilot study revealed effects of an environmental disturbance event on the phytoplankton community in Lake Lugano (Switzerland), characterized by a reduction in cytometry-based functional diversity and by a period of cyanobacterial dominance. These changes were missed by traditional limnological methods, employed in parallel to high-frequency monitoring. Modeling of phytoplankton functional diversity revealed the importance of integrated spatiotemporal data, including circadian time-lags and variability over the water column, to understand the drivers of diversity and dynamic processes. The approach described represents progress toward an automated and trait-based analysis of phytoplankton natural communities. Streamlining of high-frequency measurements may represent a resource for understanding, modeling and managing aquatic ecosystems under impact of environmental change, yielding insight into processes governing phytoplankton community resistance and resilience.


Assuntos
Ecossistema , Monitoramento Ambiental/instrumentação , Fitoplâncton/fisiologia , Cianobactérias/fisiologia , Citometria de Fluxo/instrumentação , Lagos/química , Fitoplâncton/microbiologia , Suíça , Água/química
17.
Aquat Toxicol ; 104(3-4): 230-42, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21635866

RESUMO

Metastudies have found no consistent effects of the cyanobacterial toxin microcystin on Daphnia, and there are discrepancies between field observations and experiments. Confounding factors include absence or presence of alternative high quality food or the presence of bioactive compounds, other than microcystins in cyanobacteria. Of specific interest are lipopolysaccharides (LPS) on the outer cell wall. LPS may have a number of biological effects, including reduced detoxication of microcystins in plants and animals. When grazing seston in the field, filterfeeders take up heterotrophic bacteria attached to cyanobacteria, as well as free-living bacteria. The LPS produced by heterotrophic bacteria have been shown to be much more harmful than cyanobacterial LPS. We performed two experiments in which we tested for potential synergistic effects between bacterial LPS and microcystins. Full-factorial experiments separated the main effects and interactions between (i) food quantity as well as food quality (addition of the green alga Scenedesmus), (ii) presence or absence of strains that vary in amount and composition of microcystins (microcystin free strain NIVA-CYA43, moderate microcystin producing strain NIVA-CYA140 and high microcystin producing strain PCC7820), and (iii) presence or absence of bacterial LPS on different life history traits of Daphnia galeata. We measured juvenile growth rate, age and size at first reproduction, death before first reproduction and standard carbon content of Daphnia. From the experiments we conclude that microcystin-producing Microcystis had deleterious effects on the life history of D. galeata, but especially when the availability of high quality green algal food was limited in comparison to the supply of microcystin producing strain PCC7820. In the experiment in which PCC7820 was used as microcystin-producing strain, addition of LPS lowered SCC of Daphnia, but had no effects on other life history parameters. The interaction between Microcystis strain, Microcystis concentration and LPS was highly significant in case of PCC7820, but not in case of CYA-140, indicating that the effects of LPS and its interactions with microcystin on Daphnia life history were strongly context dependent.


Assuntos
Daphnia/efeitos dos fármacos , Cadeia Alimentar , Lipopolissacarídeos/toxicidade , Microcistinas/toxicidade , Poluentes Químicos da Água/toxicidade , Animais , Daphnia/metabolismo , Sinergismo Farmacológico , Lipopolissacarídeos/metabolismo , Microcistinas/metabolismo , Estresse Fisiológico , Poluentes Químicos da Água/metabolismo
18.
Plant Physiol ; 155(3): 1445-57, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21205618

RESUMO

Physiological adaptation and genome-wide expression profiles of the cyanobacterium Synechocystis sp. strain PCC 6803 in response to gradual transitions between nitrogen-limited and light-limited growth conditions were measured in continuous cultures. Transitions induced changes in pigment composition, light absorption coefficient, photosynthetic electron transport, and specific growth rate. Physiological changes were accompanied by reproducible changes in the expression of several hundred open reading frames, genes with functions in photosynthesis and respiration, carbon and nitrogen assimilation, protein synthesis, phosphorus metabolism, and overall regulation of cell function and proliferation. Cluster analysis of the nearly 1,600 regulated open reading frames identified eight clusters, each showing a different temporal response during the transitions. Two large clusters mirrored each other. One cluster included genes involved in photosynthesis, which were up-regulated during light-limited growth but down-regulated during nitrogen-limited growth. Conversely, genes in the other cluster were down-regulated during light-limited growth but up-regulated during nitrogen-limited growth; this cluster included several genes involved in nitrogen uptake and assimilation. These results demonstrate complementary regulation of gene expression for two major metabolic activities of cyanobacteria. Comparison with batch-culture experiments revealed interesting differences in gene expression between batch and continuous culture and illustrates that continuous-culture experiments can pick up subtle changes in cell physiology and gene expression.


Assuntos
Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Regulação Bacteriana da Expressão Gênica/efeitos da radiação , Luz , Nitrogênio/farmacologia , Synechocystis/genética , Synechocystis/fisiologia , Análise por Conglomerados , Genes Bacterianos/genética , Família Multigênica/genética , Análise Espectral , Synechocystis/citologia , Synechocystis/crescimento & desenvolvimento
20.
Adv Exp Med Biol ; 619: 675-732, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18461789

RESUMO

This paper reviews the rapidly expanding literature on the ecological effects of cyanobacterial toxins. The study employs a qualitative meta-analysis from the literature examining results from a large number of independent studies and extracts general patterns from the literature or signals contradictions. The meta-analysis is set up by putting together two large tables--embodying a large and representative part of the literature (see Appendix A). The first table (Table A.1) reviews the presence (concentrations) of different cyanobacterial toxins in the tissues of various groups of aquatic biota after exposure via different routes, experimentally in the lab or via natural routes in the environment. The second table (Table A.2) reviews the dose dependent effect of toxins on biota. The great majority of studies deal with the presence and effects of microcystin, especially of the MC-LR congener. Although this may partly be justified--MC-LR is an abundant and highly toxic protein--our review also emphasizes what is known about (i) other MC congeners (a number of studies showed a preferred accumulation of the less toxic variant MC-RR in animal tissues), (ii) nodularin (data on a range of biota from studies on the Baltic Sea), (iii) neurotoxins like anatoxin-a(s), which are conspicuously often present at times when mass mortalities of birds occur, (iv) a few studies on the presence and effects of cylindrospermposin, as well as (v) the first examples of ecological effects of newly identified bioactive compounds, like microviridin-J. Data were reorganized to assess to what extent bioconcentration (uptake and concentration of toxins from the water) or biomagnification (uptake and concentration via the food) of cyanobacterial toxins occurs in ecosystems. There is little support for the occurrence of biomagnification, and this reduces the risk for biota at higher trophic levels. Rather than biomagnification biodilution seems to occur in the foodweb with toxins being subject to degradation and excretion at every level. Nevertheless toxins were present at all tropic levels, indicating that some vectorial transport must take place, and in sufficient quantities for effects to possibly occur. Feeding seemed to be the most important route for exposure of aquatic biota to cyanobacterial toxins. A fair number of studies focus on dissolved toxins, but in those studies purified toxin typically is used, and biota do not appear very sensitive to this form of exposure. More effects are found when crude cyanobacterial cell lysates are used, indicating that there may be synergistic effects between different bioactive compounds. Aquatic biota are by no means defenseless against toxic cyanobacteria. Several studies indicate that those species that are most frequently exposed to toxins in their natural environment are also the most tolerant. Protection includes behavioral mechanisms, detoxication of MC and NODLN by conjugation with glutathione, and fairly rapid depuration and excretion. A common theme in much of the ecological studies is that of modulating factors. Effects are seldom straightforward, but are dependent on factors like the (feeding) condition of the animals, environmental conditions and the history of exposure (acclimation and adaptation to toxic cyanobacteria). This makes it harder to generalize on what is known about ecological effects of cyanobacterial toxins. The paper concludes by summarizing the risks for birds, fish, macroinvertebrates and zooplankton. Although acute (lethal) effects are mentioned in the literature, mass mortalities of--especially--fish are more likely to be the result of multiple stress factors that co-occur during cyanobacterial blooms. Bivalves appear remarkably resistant, whilst the harmful effects of cyanobacteria on zooplankton vary widely and the specific contribution of toxins is hard to evaluate.


Assuntos
Toxinas Bacterianas/toxicidade , Cianobactérias/patogenicidade , Ecossistema , Eutrofização , Toxinas Marinhas/toxicidade , Microcistinas/toxicidade , Animais , Toxinas Bacterianas/administração & dosagem , Toxinas Bacterianas/análise , Toxinas Bacterianas/farmacocinética , Toxinas de Cianobactérias , Relação Dose-Resposta a Droga , Ecotoxicologia , Cadeia Alimentar , Água Doce/análise , Água Doce/microbiologia , Inativação Metabólica , Toxinas Marinhas/administração & dosagem , Toxinas Marinhas/análise , Toxinas Marinhas/farmacocinética , Microcistinas/administração & dosagem , Microcistinas/análise , Microcistinas/farmacocinética , Estresse Oxidativo , Água do Mar/análise , Água do Mar/microbiologia
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