Biosorption of chromium by live and dead cells of Bacillus nitratireducens isolated from textile effluent.

Bacillus nitratireducens was isolated from textile effluent and showed high tolerance to chromium (Cr), reaching up to a 1000 mg/L MIC value. This research was aimed at utilizing biosorbents from live and dead cells of B. nitratireducens to remove Cr from an aqueous solution. A batch biosorption test was performed, and mechanisms analysis was approached by an adsorption-desorption test, SEM-EDS, and FTIR analysis. Cr removal by dead cells in 25, 50, and 100 mg/L of Cr were 58.99 ± 0.7%, 69.8 ± 0.2%, and 82.87 ± 0.11%, respectively, while that by live cells was 73.08 ± 1.9%, 80.27 ± 6.33%, and 86.17 ± 1.93%, respectively. Live cells showed significantly higher Cr removal and adsorption capacities as compared to dead cells. In all concentrations, absorption contributed more than adsorption to the Cr removal by both live and dead cells. Absorption of Cr was subjected to occur due to passive mechanisms in dead cells while involving some active mechanisms in live cells. SEM-EDS confirmed the detection of Cr on the cell surface, while FTIR revealed the shifting of some peaks after the biosorption test, suggesting interactions between Cr and functional groups. Further TEM analysis is suggested to be conducted as a future approach to reveal the inner structure of cells and confirm the involvement of absorption mechanisms.

Effect of the number of Cyperus rotundus and medium height on the performance of batch-constructed wetland in treating aquaculture effluent.

Increasing aquaculture cultivation produces large quantities of wastewater. If not handled properly, it can have negative impacts on the environment. Constructed wetlands (CWs) are one of the phytoremediation methods that can be applied to treat aquaculture effluent. This research was aimed at determining the performance of Cyperus rotundus in removing COD, BOD, TSS, turbidity, ammonia, nitrate, nitrite, and phosphate from the batch CW system. Treatment was carried out for 30 days with variations in the number of plants (10, 15, and 20) and variations in media height (10, 12, and 14 cm). The result showed that aquaculture effluent contains high levels of organic compounds and nutrients, and C. rotundus can grow and thrive in 100% of aquaculture effluent. Besides that, the use of C. rotundus in CWs with the effect of numbers of plants and media height showed performance of COD, BOD, TSS, turbidity, ammonia, nitrate, nitrite, and phosphate with 70, 79, 90, 96, 64, 82, 92, and 48% of removal efficacy, respectively. There was no negative impact observed on C. rotundus growth after exposure to aquaculture effluent, as indicated by the increase in wet weight, dry weight, and growth rate when compared to the control. Thus, adding aquaculture effluent to CWs planted with C. rotundus supports the growth and development of plants while also performing phytoremediation.

Maximizing diesel removal from contaminated sand using Scirpus mucronatus and assessment of rhizobacteria addition effect.

Phytoremediation is one of the green technologies that is friendly to nature, utilizes fewer chemicals, and exhibits good performance. In this study, phytoremediation was used to treat diesel-contaminated sand using a local aquatic plant species, Scirpus mucronatus, by analyzing the amount of total petroleum hydrocarbons (TPHs). Optimization of diesel removal was performed according to Response Surface Methodology (RSM) using Box-Behnken Design (BBD) under pilot-scale conditions. The quadratic model showed the best fit to describe the obtained data. Actual vs. predicted values from BBD showed a total of 9.1 % error for the concentration of TPH in sand and 0 % error for the concentration of TPH in plants. Maximum TPH removal of 42.3 ± 2.1 % was obtained under optimized conditions at a diesel initial concentration of 50 mg/kg, an aeration rate of 0.48 L/min, and a retention time of 72 days. The addition of two species of rhizobacteria (Bacillus subtilis and Bacillus licheniformis) at optimum conditions increased the TPH removal to 51.9 ± 2.6 %. The obtained model and optimum condition can be adopted to treat diesel-contaminated sand within the same TPH range (50-3000 mg/kg) in sand.

Plant-based coagulants/flocculants: characteristics, mechanisms, and possible utilization in treating aquaculture effluent and benefiting from the recovered nutrients.

The increasing intensification of the aquaculture industry requires the development of new strategies to reduce the negative impacts of wastewater on the environment. Plant-based coagulants/flocculants, regarded as one of the environmentally friendly technologies for wastewater treatment, show good performance in the removal of suspended solids from wastewater. The aforementioned technology involves the utilization of plants as coagulants/flocculants in the treatment process and produces nontoxic sludge as treatment by-products. The produced sludge could be converted into valuable compounds used in agriculture. This review summarizes coagulation-flocculation by using plant-based coagulants/flocculants, its mechanisms, operational factors that control the treatment process, and its application in the treatment of wastewater, especially aquaculture effluent. Moreover, this work discusses the potential utilization of aquaculture sludge as a valuable compound used in agriculture. The presented review aims to emphasize the potential of using plant-based coagulants/flocculants in the treatment of aquaculture effluent and explore the potential of using the produced sludge as fertilizer for plants to solve problems related to sludge handling and the toxicity of inorganic coagulants in a recirculating aquaculture system. This paper concluded that utilization of recovered nutrients in the form of solids is feasible for agricultural purposes, while a hydroponic system can be used to reclaim the nutrients in the form of solution.

Assessing the effect of multiple variables on the production of bioflocculant by Serratia marcescens: Flocculating activity, kinetics, toxicity, and flocculation mechanism.

Bioflocculants gain attention as alternatives to chemical flocculants because they are more environmentally friendly and highly biodegradable. This study aims to improve the bioflocculant production by Serratia marcescens using one-variable-at-a-time (OVAT) analysis and analyze its flocculating activity performance, toxicity, and the flocculation mechanism. The effect of multiple variables including initial inoculum size, pH, mixing speed, temperature, growth medium, and incubation period was assessed through OVAT. Flocculating activity was then determined via jar test analysis, and toxicity test was performed using Daphnia magna and Daphnia pulex. The flocculation mechanism was determined via particle size distribution and zeta potential analysis. The optimum conditions for the improved bioflocculant production were as follows: 10% v/v initial inoculum size, pH 7, mixing speed of 150 rpm, room temperature, nutrient broth medium, and 72 h of incubation period. Scanning electron microscopy showed flake-like intact structure with coarse surface. The produced bioflocculant showed flocculating activity of 48% in 5227 ± 580 NTU initial kaolin turbidity with 1 mg/L concentration and 5% v/v dosage of bioflocculant, following the second-order kinetics. Toxicity test to D. magna and D. pulex showed the 48 h LC50 values of 8.06 and 6.42 g/L, respectively; these values are greatly higher than the fabricated chemical flocculants. The flocculation process using bioflocculant produced by S. marcescens was suggested to occur via bridging mechanism because it greatly affected the particle size distribution. Results indicated that bioflocculant produced by S. marcescens is much environmentally friendly and has great potential for turbidity removal in water/wastewater.

Practical limitations of bioaugmentation in treating heavy metal contaminated soil and role of plant growth promoting bacteria in phytoremediation as a promising alternative approach.

Bioaugmentation, the addition of cultured microorganisms to enhance the currently existing microbial community, is an option to remediate contaminated areas. Several studies reported the success of the bioaugmentation method in treating heavy metal contaminated soil, but concerns related to the applicability of this method in real-scale application were raised. A comprehensive analysis of the mechanisms of heavy metal treatment by microbes (especially bacteria) and the concerns related to the possible application in the real scale were juxtaposed to show the weakness of the claim. This review proposes the use of bioaugmentation-assisted phytoremediation in treating heavy metal contaminated soil. The performance of bioaugmentation-assisted phytoremediation in treating heavy metal contaminated soil as well as the mechanisms of removal and interactions between plants and microbes are also discussed in detail. Bioaugmentation-assisted phytoremediation shows greater efficiencies and performs complete metal removal from soil compared with only bioaugmentation. Research related to selection of hyperaccumulator species, potential microbial species, analysis of interaction mechanisms, and potential usage of treating plant biomass after treatment are suggested as future research directions to enhance this currently proposed topic.

Ecological impacts of ballast water loading and discharge: insight into the toxicity and accumulation of disinfection by-products.

Since the implementation of the International Maritime Organization 2004 regulation, most ships have been equipped with on-dock ballast water treatment. While this method is effective in solving the invasive alien species problem, concerns are raised due to the potential release of disinfection by-products (DBPs) as the result of the chemical treatment. This review paper aims to summarize the history of ballast water management (BWM) and the currently used on-dock technology. Chlorination, oxidation, and ozonation are highlighted as the most currently applied methods to treat ballast water on-dock. This paper then focuses on the potential release of toxic DBPs as the result of the selected corresponding treatment methods. Tri-halo methane, haloacetic acid, and several acetic acid-related compounds are emphasized as toxic DBPs with concentrations reaching more than 10 µg/L. The potential toxicities of DBPs, including acute toxicity, carcinogenicity, genotoxicity, and mutagenicity, to aquatic organisms, are then discussed in detail. Future research directions related to the advanced treatment of DBPs before final discharge and analysis of DBPs in coastal sediments, which are barely studied at present, are suggested to enhance the current knowledge on the fate and the ecological impact of BWM.

Integrated emergent-floating planted reactor for textile effluent: Removal potential, optimization of operational conditions and potential forthcoming waste management strategy.

Native emergent and floating plants; local reed grass (Phragmites karka) and water hyacinth (Eichhornia crassipes), respectively, were used to treat textile wastewater using an integrated emergent-floating planted reactor (IEFPR) system at hydraulic retention times (HRTs) of 8, 14, and 19 days. Real textile effluent having characteristics of 1686.3 ADMI for colour, 535 mg/L for total suspended solid (TSS), 647.7 mg/L for chemical oxygen demand (COD) and 124 mg/L for biochemical oxygen demand (BOD) was used throughout this study. The IEFPR system experienced maximum removal of colour (94.8%, HRT 14 days, day 3), TSS (92.7%, HRT 19 days, day 7), and COD (96.6%, HRT 8 days, day 5) at different HRT and exposure time. The process conditions (HRT and exposure time) were optimized for maximum colour, TSS and COD removal from textile effluent by employing response surface methodology (RSM). The optimization has resulted 100% removal of colour, 87% removal of TSS and 100% removal of COD at HRT of 8 days and exposure time of 5 days, with 0.984 desirability. The integrated plant-assisted treatment system showed reliable performance in treating textile wastewater at optimum operational conditions to improve effluent quality before disposal into water bodies or being recycled into the process. The potential of phytoremediator (produced plant biomass) to be utilized as resources for bioenergy or to be converted into value added products (adsorbent or biochar) provides an alternative to management strategy for better environmental sustainability.

Comparative performance of Scirpus grossus for phytotreating mixed dye wastewater in batch and continuous pilot subsurface constructed wetland systems.

Dye is one of the pollutants found in water bodies because of the increased growth of the textile industry. In this study, Scirpus grossus was planted inside a constructed wetland to treat mixed dye (methylene blue and methyl orange)-containing wastewater under batch and continuous modes. The plants were exposed to various concentrations (0, 50, 75, and 100 mg/L) of mixed dye for 72 days (with hydraulic retention time of 7 days for the continuous system). Biological oxygen demand, chemical oxygen demand, total organic carbon, pH, temperature, ionic content, and plant growth parameters were measured. Results showed that S. grossus can withstand all the tested dye concentrations until the end of the treatment period. Color removal efficiencies of 86, 84, and 75% were obtained in batch mode, whereas 90%, 85%, and 79% were obtained in continuous mode for 50, 75, and 100 mg/L dye concentrations, respectively. Fourier-transform infrared analysis confirmed the transformation of dye compounds after treatment and scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy analysis showed that most of the intermediate compounds were not absorbed into plants but adsorbed onto the surface of the root structure.

What compound inside biocoagulants/bioflocculants is contributing the most to the coagulation and flocculation processes?

Biocoagulants and bioflocculants are alternative items that can be used to substitute the utilization of common-chemical coagulants and flocculants. Biocoagulants/bioflocculants can be extracted from animals, microorganisms, and plants. Moreover, biocoagulants/bioflocculants have specific characteristics that contribute to the coagulation and flocculation processes. The active compounds inside biocoagulants/bioflocculants vary and correspond to the specific working mechanisms, including charge neutralization, sweep coagulation, adsorption, bridging, and patch flocculation. This review paper summarizes the characteristics of biocoagulants/bioflocculants from different sources and its performance in treating various pollutants. Furthermore, this paper discusses the most contributing compounds and functional groups of biocoagulants/bioflocculants that can be related to their working mechanisms. Several functional groups and compounds in biocoagulants/bioflocculants are highlighted in this review article, as well as the correlation between the highlighted groups/compounds to the aforementioned coagulation-flocculation mechanisms. In addition, current knowledge gaps in the study of biocoagulants/bioflocculants and future approaches that may serve as research directions are also emphasized. This review article is expected to shed information on the characteristics of biocoagulants/bioflocculants, which may then become a focus in the optimization to obtain higher performance in future application of coagulation-flocculation processes.

Macrophytes as wastewater treatment agents: Nutrient uptake and potential of produced biomass utilization toward circular economy initiatives.

Macrophytes have been widely used as agents in wastewater treatment. The involvement of plants in wastewater treatment cannot be separated from wetland utilization. As one of the green technologies in wastewater treatment plants, wetland exhibits a great performance, especially in removing nutrients from wastewater before the final discharge. It involves the use of plants and consequently produces plant biomasses as treatment byproducts. The produced plant biomasses can be utilized or converted into several valuable compounds, but related information is still limited and scattered. This review summarizes wastewater's nutrient content (macro and micronutrient) that can support plant growth and the performance of constructed wetland (CW) in performing nutrient uptake by using macrophytes as treatment agents. This paper further discusses the potential of the utilization of the produced plant biomasses as bioenergy production materials, including bioethanol, biohydrogen, biogas, and biodiesel. This paper also highlights the conversion of plant biomasses into animal feed, biochar, adsorbent, and fertilizer, which may support clean production and circular economy efforts. The presented review aims to emphasize and explore the utilization of plant biomasses and their conversion into valuable products, which may solve problems related to plant biomass handling during the adoption of CW in wastewater treatment plants.

Challenges and Opportunities of Biocoagulant/Bioflocculant Application for Drinking Water and Wastewater Treatment and Its Potential for Sludge Recovery.

The utilization of metal-based conventional coagulants/flocculants to remove suspended solids from drinking water and wastewater is currently leading to new concerns. Alarming issues related to the prolonged effects on human health and further pollution to aquatic environments from the generated nonbiodegradable sludge are becoming trending topics. The utilization of biocoagulants/bioflocculants does not produce chemical residue in the effluent and creates nonharmful, biodegradable sludge. The conventional coagulation-flocculation processes in drinking water and wastewater treatment, including the health and environmental issues related to the utilization of metal-based coagulants/flocculants during the processes, are discussed in this paper. As a counterpoint, the development of biocoagulants/bioflocculants for drinking water and wastewater treatment is intensively reviewed. The characterization, origin, potential sources, and application of this green technology are critically reviewed. This review paper also provides a thorough discussion on the challenges and opportunities regarding the further utilization and application of biocoagulants/bioflocculants in water and wastewater treatment, including the importance of the selection of raw materials, the simplification of extraction processes, the application to different water and wastewater characteristics, the scaling up of this technology to a real industrial scale, and also the potential for sludge recovery by utilizing biocoagulants/bioflocculants in water/wastewater treatment.

Bioaugmentation of Vibrio alginolyticus in phytoremediation of aluminium-contaminated soil using Scirpus grossus and Thypa angustifolia.

This research analyses the performance of bacteria-assisted phytoremediation of aluminium (Al)-contaminated soil using native Indonesian plants namely, Scirpus grossus and Thypa angustifolia. A range finding test (RFT) was carried out for 14 days to obtain the tolerable Al concentration for both plants. A total of 2% and 5% (v/v) of Vibrio alginolyticus were bioaugmented during the 28-day phytoremediation test to enhance the overall Al removal. Result of the RFT showed that both plants can tolerate up to 500 mg/kg Al concentration. The addition of V. alginolyticus to the reactors resulted in a significant increment of Al removal from the contaminated soil (p < 0.05). Such addition of V. alginolyticus increased the Al removal by up to 14.0% compared with that without-bacteria addition. The highest Al removal was obtained for S. grossus with 5% V. alginolyticus with an efficiency of 35.1% from 500 mg/kg initial concertation. T. angustifolia with 500 mg/kg initial concentration showed the highest removal of 26.2% by the addition of 5% V. alginolyticus. The increase of Al removal by the bioaugmentation of V. alginolyticus was due to the interaction in the plant's rhizosphere. Exudates of both plants provided a good environment for bacteria to live in the root area. Meanwhile, the bacteria increased the bioavailability of Al to be further extracted by plants. Certain mechanisms, such as rhizostabilisation, phytostimulation and phytoextraction, were considered to be the main processes that occurred during the treatment. S. grossus and T. angustifolia displayed promising ability to act as Al hyperaccumulators with bioaccumulation factor values up to 5.308 and 3.068, respectively. Development of the design of the ex-situ soil phytoremediation reactors is suggested as a future research direction because it can significantly enhance the current obtained finding.

Dual function of Lemna minor and Azolla pinnata as phytoremediator for Palm Oil Mill Effluent and as feedstock.

In this study, two native duckweeds (Lemna minor and Azolla pinnata) were cultivated in Palm Oil Mill Effluent (POME) to extract nutrients from the effluent. Five grams of A. pinnata and 2 g of L. minor were transferred to 2 L POME (Initial concentrations: 198 mg/L COD, 4.3 mg/L nitrates, pH 9.53, 4 mg/L phosphate, 2.98 mg/L ammonia) with four different dilutions (2.5%, 5%, 10%, 15%) under greenhouse conditions. Samples of POME were taken every two days up to 10 days. Growth parameter, phosphate, ammonia, nitrates, pH, and COD were monitored within 10 days to select the most suitable growth medium for both plants. Results showed that 2.5% POME dilution had positive effect on L. minor growth and A. pinnata (wet weight increased by 8.7 g and 9.8 g, respectively), with all plants able to survive until the final day of exposure. The highest removal of ammonia was accomplished in 5% POME dilution by A. pinnata (98%) and L. minor (95.5%). The maximum phosphate removal was obtained in 10% POME dilution with 93.3% removal by A. pinnata and 86.7% by L. minor. Significant COD removal in 15% POME was obtained by L. minor (78%) and A. pinnata (66%). Both plants responded positively to the phytoremediation process, especially for A. pinnata which showed significant decreases in all parameters. The nutrient extraction by both plants from POME showed a positive effect on growth parameter, which has further promising potential to be used as animal feedstock.

Phytoremediation of methylene blue using duckweed (Lemna minor).

Azo dyes are the largest class of synthetic dyes and are utilized in several industries. Effluents containing dyes are released to the environment and pose harm to humans who might be exposed to these contaminants. This study aims to investigate the removal of methylene blue (MB) dye using duckweed (Lemna minor). L. minor (2 g) was exposed into 50 mg/L of MB dyes for 24 h. The absorbance values were measured at 0, 0.5, 1, 2, 3, 4, 5, 6, and 24 h with a maximum wavelength of 665 nm. The dye removal percentage and relative growth rate of L. minor during exposure to MB were observed. The removal percentage was 80.56 ± 0.44% for 24 h with a relative growth rate of 0.006/h. L. minor has potential as a phytoremediation agent to remove dyes from wastewater.

Aluminium removal and recovery from wastewater and soil using isolated indigenous bacteria.

This paper elucidates the capability of isolated indigenous bacteria to remove aluminium from wastewater and soil. Two indigenous species of Brochothrix thermosphacta and Vibrio alginolyticus were isolated from an aluminium-contaminated site. These two species were used to treat aluminium-containing wastewater and contaminated soil using the bioaugmentation method. B. thermosphacta showed the highest aluminium removal of 57.87 ±â€¯0.45% while V. alginolyticus can remove aluminium up to 59.72 ±â€¯0.33% from wastewater. For aluminium-contaminated soil, B. thermosphacta and V. alginolyticus, showed a highest removal of only 4.58 ±â€¯0.44% and 5.48 ±â€¯0.58%, respectively. The bioaugmentation method is more suitable to be used to treat aluminium in wastewater compared to contaminated soil. The produced biomass separation after wastewater treatment was so much easier and applicable, compared to the produced biomass handling from contaminated soil treatment. A 48.55 ±â€¯2.45% and 40.12 ±â€¯4.55% of aluminium can be recovered from B. thermosphacta and V. alginolyticus biomass, respectively, with 100 mg/L initial aluminium concentration in wastewater.

Characterization of mercury-reducing potential bacteria isolated from Keputih non-active sanitary landfill leachate, Surabaya, Indonesia under different saline conditions.

The objectives of this research were to identify the capability of bacteria isolated from a non-active sanitary landfill to remove mercury under different saline conditions and to understand the removal kinetics. The mercury concentrations used in the minimum inhibitory concentration (MIC) test were 0, 5, 10, 15 and 20 mg/L. The capability of one selected bacterium from the MIC test to remove mercury under different saline conditions (0, 10, 20, and 30‰) was also tested. Five indigenous bacteria were isolated from the Keputih non-active sanitary landfill, Surabaya, Indonesia. The MICs of mercury for FA-1, FA-2, FA-3, FA-4, and FA-5 were 5, 10, 5, 5, and 5 mg/L, respectively. Based on biochemical characterization, FA-2 was identified as Pseudomonas aeruginosa. The isolate of P. aeruginosa was capable of removing Hg under different saline conditions. The optimum saline condition for P. aeruginosa to remove Hg was 10‰, with a removal percentage of 99.7 ±â€¯0.18% following pseudo-second-order kinetics (R2 = 0.9939) with k2 = 2.0059 mg substrate/g adsorbent/hr. Hence, isolated P. aeruginosa showed potential for the bioremediation of mercury-contaminated areas.

Kinetics of aluminium removal by locally isolated Brochothrix thermosphacta and Vibrio alginolyticus.

The emergence of the aluminium recycling industry has led to an increase in aluminium-containing wastewater discharge to the environment. Biological treatment of metal is one of the solutions that can be provided as green technology. Screening tests showed that Brochothrix thermosphacta and Vibrio alginolyticus have the potential to remove aluminium from wastewater. Brochothrix thermosphacta removed up to 49.60%, while Vibrio alginolyticus was capable of removing up to 59.72% of 100 mg/L aluminium in acidic conditions. The removal of aluminium by V. alginolyticus was well fitted with pseudo-first-order kinetics (k1 = 0.01796/min), while B. thermosphacta showed pseudo-second-order kinetics (k2 = 0.125612 mg substrate/g adsorbent. hr) in the process of aluminium removal. V. alginolyticus had a higher rate constant under acidic conditions, while B. thermosphacta had a higher rate constant under neutral pH conditions.