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1.
Cancers (Basel) ; 11(2)2019 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-30813491

RESUMO

Neoantigens derived from tumor-specific genetic mutations might be suitable targets for cancer immunotherapy because of their high immunogenicity. In the current study, we evaluated the immunogenicity of 10 driver mutations that are frequently expressed in various cancers using peripheral blood mononuclear cells from healthy donors (n = 25). Of the 10 synthetic peptides (27-mer) derived from these mutations, the six peptides from KRAS-G12D, KRAS-G12R, KRAS-G13D, NRAS-Q61R, PIK3CA-H1047R, and C-Kit-D816V induced T cell responses, suggesting that frequent driver mutations are not always less immunogenic. In particular, immune responses to PIK3CA-H1047R, C-Kit-D816V, KRAS-G13D, and NRAS-Q61R were observed in more than 10% of the donors. All six peptides induced human leukocyte antigen (HLA) class II-restricted CD4⁺ T cell responses; notably, PIK3CA-H1047R contained at least two different CD4⁺ T cell epitopes restricted to different HLA class II alleles. In addition, PIK3CA-H1047R and C-Kit-D816V induced antigen-specific CD8⁺ T cells as well, indicating that they might contain both HLA class I- and class II-restricted epitopes. Since the identified neoantigens might be shared by patients with various types of cancers and are not easily lost due to immune escape, they have the potential to be promising off-the-shelf cancer immunotherapy targets in patients with the corresponding mutations.

2.
PLoS One ; 11(11): e0166381, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27832201

RESUMO

We have reported a correlation between hypothalamic expression of Creb-binding protein (Cbp) and lifespan, and that inhibition of Cbp prevents protective effects of dietary restriction during aging, suggesting that hypothalamic Cbp plays a role in responses to nutritional status and energy balance. Recent GWAS and network analyses have also implicated Cbp as the most connected gene in protein-protein interactions in human Type 2 diabetes. The present studies address mechanisms mediating the role of Cbp in diabetes by inhibiting hypothalamic Cbp using a Cre-lox strategy. Inhibition of hypothalamic Cbp results in profound obesity and impaired glucose homeostasis, increased food intake, and decreased body temperature. In addition, these changes are accompanied by molecular evidence in the hypothalamus for impaired leptin and insulin signaling, a shift from glucose to lipid metabolism, and decreased Pomc mRNA, with no effect on locomotion. Further assessment of the significance of the metabolic switch demonstrated that enhanced expression of hypothalamic Cpt1a, which promotes lipid metabolism, similarly resulted in increased body weight and reduced Pomc mRNA.


Assuntos
Proteína de Ligação a CREB/metabolismo , Hipotálamo/metabolismo , Obesidade/metabolismo , Animais , Temperatura Corporal , Fator Neurotrófico Derivado do Encéfalo/genética , Proteína de Ligação a CREB/genética , Carnitina O-Palmitoiltransferase/genética , Diabetes Mellitus Tipo 2/genética , Diabetes Mellitus Tipo 2/metabolismo , Diabetes Mellitus Tipo 2/patologia , Regulação para Baixo , Ingestão de Alimentos , Metabolismo Energético , Feminino , Regulação da Expressão Gênica , Glucose/metabolismo , Humanos , Hipotálamo/patologia , Insulina/metabolismo , Leptina/metabolismo , Metabolismo dos Lipídeos , Masculino , Camundongos , Obesidade/genética , Obesidade/patologia , Pró-Opiomelanocortina/genética , Transdução de Sinais , Aumento de Peso
3.
J Interferon Cytokine Res ; 35(4): 302-12, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25406893

RESUMO

Surgical trauma induces immune and stress responses although its effects on postsurgical inflammatory and stress gene expression remain poorly characterized. This study sought to improve current scientific knowledge by investigating the effects of laparotomy on mouse blood cell inflammatory and stress gene expression. Three-month-old male C57BL/6J mice were subjected to 2% isoflurane or 2% isoflurane with laparotomy and sacrificed 4 h postintervention. Blood was collected and blood cell expression of 158 genes central to inflammatory and stress responses was assayed using quantitative polymerase chain reaction arrays. Mice subjected to isoflurane with laparotomy, compared with mice receiving isoflurane alone, had >2-fold upregulation of genes in inflammation (Osm, IL1rn, IL1b, and Csf1), oxidative stress (Hmox1), heat shock (Hspa1b), growth arrest (Cdkn1a), and DNA repair (Ugt1a2). These genes demonstrated similar expression patterns by Pearson correlation and cluster analysis. Thus, laparotomy induces coordinated, postsurgical blood cell expression of unique inflammatory and stress genes whose roles in influencing surgical outcomes need further investigation.


Assuntos
Células Sanguíneas/metabolismo , Regulação da Expressão Gênica , Laparotomia , Estresse Fisiológico/genética , Animais , Células Sanguíneas/efeitos dos fármacos , Perfilação da Expressão Gênica , Inflamação/genética , Isoflurano/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Estresse Fisiológico/efeitos dos fármacos
4.
Endocrinology ; 154(9): 3054-66, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23709088

RESUMO

A major barrier in reversing diabetic complications is that molecular and pathologic effects of elevated glucose persist despite normalization of glucose, a phenomenon referred to as metabolic memory. In the present studies we have investigated the effects of elevated glucose on Schwann cells, which are implicated in diabetic neuropathy. Using quantitative PCR arrays for glucose and fatty acid metabolism, we have found that chronic (>8 wk) 25 mM high glucose induces a persistent increase in genes that promote glycolysis, while inhibiting those that oppose glycolysis and alternate metabolic pathways such as fatty acid metabolism, the pentose phosphate pathway, and trichloroacetic acid cycle. These sustained effects were associated with decreased peroxisome proliferator-activated receptor (PPAR)γ binding and persistently increased reactive oxygen species, cellular NADH, and altered DNA methylation. Agonists of PPARγ and PPARα prevented select effects of glucose-induced gene expression. These observations suggest that Schwann cells exhibit features of metabolic memory that may be regulated at the transcriptional level. Furthermore, targeting PPAR may prevent metabolic memory and the development of diabetic complications.


Assuntos
Metabolismo Energético/efeitos dos fármacos , Fenofibrato/farmacologia , Glucose/metabolismo , Hipolipemiantes/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Receptores Ativados por Proliferador de Peroxissomo/agonistas , Células de Schwann/efeitos dos fármacos , Animais , Linhagem Celular , Ciclo do Ácido Cítrico/efeitos dos fármacos , Metilação de DNA/efeitos dos fármacos , Neuropatias Diabéticas/metabolismo , Neuropatias Diabéticas/prevenção & controle , Fenofibrato/uso terapêutico , Perfilação da Expressão Gênica , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Glicólise/efeitos dos fármacos , Hiperglicemia/metabolismo , Hipoglicemiantes/farmacologia , Hipolipemiantes/uso terapêutico , Metabolismo dos Lipídeos/efeitos dos fármacos , Camundongos , Terapia de Alvo Molecular , Proteínas do Tecido Nervoso/antagonistas & inibidores , Proteínas do Tecido Nervoso/biossíntese , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/metabolismo , Via de Pentose Fosfato/efeitos dos fármacos , Receptores Ativados por Proliferador de Peroxissomo/metabolismo , Células de Schwann/metabolismo
5.
J Child Neurol ; 28(8): 1009-14, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23680948

RESUMO

Accumulating evidence suggests that low-carbohydrate, high-fat diets are safe and effective to reduce glycemia in diabetic patients without producing significant cardiovascular risks. Most of these studies have been carried out specifically restricting carbohydrates, which tends to lead to increased protein intake, thus reducing the ketosis. However, diets that limit protein as well as carbohydrates, entailing a composition very high in fat, appear even more effective to reduce glucose and whole-body glucose metabolism in humans. In animal models, low-carbohydrate, high-protein diets do not produce ketosis or reduce glycemia but rather cause obesity. However, limiting both protein and carbohydrates as in a classic ketogenic diet remarkably reduces blood glucose in animal models of type 1 and type 2 diabetes and reverses diabetic nephropathy. Future studies should assess if ketogenic diets would be effective to reverse diabetic complications in humans.


Assuntos
Complicações do Diabetes/dietoterapia , Dieta Cetogênica/métodos , Obesidade/dietoterapia , Animais , Glicemia/metabolismo , Modelos Animais de Doenças , Metabolismo Energético , Humanos , Obesidade/sangue
6.
Endocrinol Metab Clin North Am ; 42(1): 67-80, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23391240

RESUMO

All organisms must adapt to changing nutrient availability, with nutrient surplus promoting glucose metabolism and nutrient deficit promoting alternative fuels (in mammals, mainly free fatty acids). A major function of glucose-sensing neurons in the hypothalamus is to regulate blood glucose. When these neurons sense glucose levels are too low, they activate robust counterregulatory responses to enhance glucose production, primarily from liver, and reduce peripheral metabolism. Some hypothalamic neurons can metabolize free fatty acids via ß-oxidation, and ß-oxidation generally opposes effects of glucose on hypothalamic neurons. Thus hypothalamic ß-oxidation promotes obese phenotypes, including enhanced hepatic glucose output.


Assuntos
Ácidos Graxos não Esterificados/metabolismo , Hipotálamo/metabolismo , Neurônios/metabolismo , Animais , Glucose/metabolismo , Humanos , Hipotálamo/citologia , Fígado/metabolismo
7.
Am J Physiol Endocrinol Metab ; 302(8): E987-91, 2012 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-22318949

RESUMO

To discover hypothalamic genes that might play a role in regulating energy balance, we carried out a microarray screen for genes induced by a 48-h fast in male C57Bl/6J mouse hypothalamus. One such gene was Fkbp51 (FK506 binding protein 5; Locus NP_034350). The product of this gene is of interest because it blocks glucocorticoid action, suggesting that fasting-induced elevation of this gene in the hypothalamus may reduce glucocorticoid negative feedback, leading to elevated glucocorticoid levels, thus promoting obese phenotypes. Subsequent analysis demonstrated that a 48-h fast induces Fkbp51 in ventromedial, paraventricular, and arcuate hypothalamic nuclei of mice and rats. To assess if hypothalamic Fkbp51 promotes obesity, the gene was transferred to the hypothalamus via an adeno-associated virus vector. Within 2 wk following Fkbp51 overexpression, mice on a high-fat diet exhibited elevated body weight, without hyperphagia, relative to mice receiving the control mCherry vector. Body weight remained elevated for more than 8 wk and was associated with elevated corticosterone and impaired glucose tolerance. These studies suggest that elevated hypothalamic Fkbp51 promotes obese phenotypes.


Assuntos
Jejum/metabolismo , Hipotálamo/metabolismo , Obesidade/metabolismo , Proteínas de Ligação a Tacrolimo/metabolismo , Regulação para Cima , Animais , Núcleo Arqueado do Hipotálamo/metabolismo , Corticosterona/sangue , Ingestão de Energia , Perfilação da Expressão Gênica , Intolerância à Glucose/sangue , Intolerância à Glucose/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Obesidade/sangue , Análise de Sequência com Séries de Oligonucleotídeos , Núcleo Hipotalâmico Paraventricular/metabolismo , RNA Mensageiro/metabolismo , Ratos , Ratos Sprague-Dawley , Proteínas de Ligação a Tacrolimo/antagonistas & inibidores , Proteínas de Ligação a Tacrolimo/genética , Núcleo Hipotalâmico Ventromedial/metabolismo , Aumento de Peso
8.
PLoS One ; 6(11): e27762, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-22114686

RESUMO

Screening a library of drugs with known safety profiles in humans yielded 30 drugs that reliably protected mammalian neurons against glucose toxicity. Subsequent screening demonstrated that 6 of these 30 drugs increase lifespan in C. elegans: caffeine, ciclopirox olamine, tannic acid, acetaminophen, bacitracin, and baicalein. Every drug significantly reduced the age-dependent acceleration of mortality rate. These protective effects were blocked by RNAi inhibition of cbp-1 in adults only, which also blocks protective effects of dietary restriction. Only 2 drugs, caffeine and tannic acid, exhibited a similar dependency on DAF-16. Caffeine, tannic acid, and bacitracin also reduced pathology in a transgenic model of proteotoxicity associated with Alzheimer's disease. These results further support a key role for glucose toxicity in driving age-related pathologies and for CBP-1 in protection against age-related pathologies. These results also provide novel lead compounds with known safety profiles in human for treatment of age-related diseases, including Alzheimer's disease and diabetic complications.


Assuntos
Envelhecimento/efeitos dos fármacos , Doença de Alzheimer/prevenção & controle , Proteínas de Caenorhabditis elegans/metabolismo , Aprovação de Drogas , Glucose/toxicidade , Histona Acetiltransferases/metabolismo , Neurônios/efeitos dos fármacos , Preparações Farmacêuticas/administração & dosagem , Fatores de Transcrição/metabolismo , Doença de Alzheimer/induzido quimicamente , Doença de Alzheimer/genética , Animais , Animais Geneticamente Modificados , Caenorhabditis elegans/genética , Caenorhabditis elegans/crescimento & desenvolvimento , Caenorhabditis elegans/metabolismo , Proteínas de Caenorhabditis elegans/antagonistas & inibidores , Proteínas de Caenorhabditis elegans/genética , Modelos Animais de Doenças , Fatores de Transcrição Forkhead , Histona Acetiltransferases/antagonistas & inibidores , Histona Acetiltransferases/genética , Humanos , Neurônios/citologia , RNA Interferente Pequeno/genética , Transdução de Sinais/efeitos dos fármacos , Taxa de Sobrevida , Fatores de Transcrição/antagonistas & inibidores , Fatores de Transcrição/genética , Estados Unidos , United States Food and Drug Administration
9.
PLoS One ; 6(4): e18604, 2011 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-21533091

RESUMO

Intensive insulin therapy and protein restriction delay the development of nephropathy in a variety of conditions, but few interventions are known to reverse nephropathy. Having recently observed that the ketone 3-beta-hydroxybutyric acid (3-OHB) reduces molecular responses to glucose, we hypothesized that a ketogenic diet, which produces prolonged elevation of 3-OHB, may reverse pathological processes caused by diabetes. To address this hypothesis, we assessed if prolonged maintenance on a ketogenic diet would reverse nephropathy produced by diabetes. In mouse models for both Type 1 (Akita) and Type 2 (db/db) diabetes, diabetic nephropathy (as indicated by albuminuria) was allowed to develop, then half the mice were switched to a ketogenic diet. After 8 weeks on the diet, mice were sacrificed to assess gene expression and histology. Diabetic nephropathy, as indicated by albumin/creatinine ratios as well as expression of stress-induced genes, was completely reversed by 2 months maintenance on a ketogenic diet. However, histological evidence of nephropathy was only partly reversed. These studies demonstrate that diabetic nephropathy can be reversed by a relatively simple dietary intervention. Whether reduced glucose metabolism mediates the protective effects of the ketogenic diet remains to be determined.


Assuntos
Nefropatias Diabéticas/dietoterapia , Dieta Cetogênica , Animais , Modelos Animais de Doenças , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL
10.
Brain Res ; 1280: 77-83, 2009 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-19445909

RESUMO

In rats and humans estradiol attenuates neuroendocrine responses to hypoglycemia. Since neuroendocrine responses to hypoglycemia are mediated by hypothalamic neurons, we assessed if estradiol attenuates hypoglycemia-induced gene expression in the hypothalamus in female ovariectomized mice. As expected, estradiol-implanted ovariectomized mice exhibited increased plasma estradiol, increased uterine weight, decreased body weight, decreased visceral adiposity, and enhanced glucose tolerance with decreased plasma insulin. Estradiol-implanted mice exhibited attenuated hypoglycemia-induced gene expression of both glucose transporter 1 (Glut1) and inhibitor of kappa beta signaling (IkappaB) in the hypothalamus but not in the liver. Estradiol also attenuated hypoglycemia-induced plasma glucagon, pituitary proopiomelanocortin (POMC), and adrenal c-fos, consistent with impaired counterregulatory responses to hypoglycemia. In addition, estradiol inhibited hypothalamic expression of carnitine palmitoyltransferase (CPT1a and CPT1c) and pyruvate dehydrogenase kinase 4 (PDK4), effects that would be expected to enhance the accumulation of long-chain fatty acids and glycolysis. Taken together, these findings suggest hypothalamic mechanisms mediating attenuation of hypoglycemia-induced neuroendocrine responses.


Assuntos
Estradiol/farmacologia , Estrogênios/farmacologia , Hipoglicemia/metabolismo , Hipotálamo/efeitos dos fármacos , Hipotálamo/metabolismo , Adiposidade/efeitos dos fármacos , Animais , Peso Corporal/efeitos dos fármacos , Carnitina O-Palmitoiltransferase/metabolismo , Estradiol/sangue , Estrogênios/sangue , Feminino , Expressão Gênica/efeitos dos fármacos , Teste de Tolerância a Glucose , Transportador de Glucose Tipo 1/metabolismo , Hipoglicemia/tratamento farmacológico , Hipoglicemia/genética , Proteínas I-kappa B/metabolismo , Insulina/sangue , Camundongos , Camundongos Endogâmicos C57BL , Tamanho do Órgão , Ovariectomia , Proteínas Serina-Treonina Quinases/metabolismo , Piruvato Desidrogenase Quinase de Transferência de Acetil , Distribuição Aleatória , Útero/efeitos dos fármacos , Útero/patologia
11.
Endocrinology ; 149(2): 703-10, 2008 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-17974626

RESUMO

The regulation of neuroendocrine electrical activity and gene expression by glucose is mediated through several distinct metabolic pathways. Many studies have implicated AMP and ATP as key metabolites mediating neuroendocrine responses to glucose, especially through their effects on AMP-activated protein kinase (AMPK), but other studies have suggested that glycolysis, and in particular the cytoplasmic conversion of nicotinamide adenine dinucleotide (NAD+) to reduced NAD (NADH), may play a more important role than oxidative phosphorylation for some effects of glucose. To address these molecular mechanisms further, we have examined the regulation of agouti-related peptide (AgRP) in a clonal hypothalamic cell line, N-38. AgRP expression was induced monotonically as glucose concentrations decreased from 10 to 0.5 mm glucose and with increasing concentrations of glycolytic inhibitors. However, neither pyruvate nor 3-beta-hydroxybutyrate mimicked the effect of glucose to reduce AgRP mRNA, but on the contrary, produced the opposite effect of glucose and actually increased AgRP mRNA. Nevertheless, 3beta-hydroxybutyrate mimicked the effect of glucose to increase ATP and to decrease AMPK phosphorylation. Similarly, inhibition of AMPK by RNA interference increased, and activation of AMPK decreased, AgRP mRNA. Additional studies demonstrated that neither the hexosamine nor the pentose/carbohydrate response element-binding protein pathways mediate the effects of glucose on AgRP expression. These studies do not support that either ATP or AMPK mediate effects of glucose on AgRP in this hypothalamic cell line but support a role for glycolysis and, in particular, NADH. These studies support that cytoplasmic or nuclear NADH, uniquely produced by glucose metabolism, mediates effects of glucose on AgRP expression.


Assuntos
Proteína Relacionada com Agouti/genética , Glicólise/fisiologia , Hipotálamo/citologia , Neurônios/metabolismo , Fosforilação Oxidativa , Proteínas Quinases Ativadas por AMP , Trifosfato de Adenosina/metabolismo , Animais , Linhagem Celular , Desoxiglucose/farmacologia , Interações Medicamentosas , Inibidores Enzimáticos/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica/fisiologia , Glucosamina/farmacologia , Glucose/farmacologia , Gliceraldeído-3-Fosfato Desidrogenases/antagonistas & inibidores , Gliceraldeído-3-Fosfato Desidrogenases/metabolismo , Hexosaminas/metabolismo , Iodoacetatos/farmacologia , Corpos Cetônicos/farmacologia , Camundongos , Complexos Multienzimáticos/antagonistas & inibidores , Complexos Multienzimáticos/genética , Complexos Multienzimáticos/metabolismo , NAD/metabolismo , Neurônios/citologia , Via de Pentose Fosfato/fisiologia , Proteínas Serina-Treonina Quinases/antagonistas & inibidores , Proteínas Serina-Treonina Quinases/genética , Proteínas Serina-Treonina Quinases/metabolismo , Ácido Pirúvico/farmacologia , Interferência de RNA
12.
Appetite ; 48(2): 135-8, 2007 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-17141367

RESUMO

High-fat diets produce obesity in part because, per calorie, glucose produces greater post-prandial thermogenesis than lipids, an effect probably mediated by glucose-sensing neurons. A very low-carbohydrate/high-fat/high-protein Atkins-type diet produces obesity but is marginally ketogenic in mice. In contrast, high-sucrose/low-fat diets, and very low-carbohydrate/high-fat/low-protein (anti-epileptic) ketogenic diets reverse diet-induced obesity independent of caloric intake. We propose that a non-ketogenic high-fat diet reduces glucose metabolism and signaling in glucose-sensing neurons, thereby reducing post-prandial thermogenesis, and that a ketogenic high-fat diet does not reduce glucose signaling, thereby preventing and/or reversing obesity.


Assuntos
Dieta com Restrição de Carboidratos , Dieta Redutora , Metabolismo Energético/fisiologia , Obesidade/dietoterapia , Obesidade/etiologia , Glicemia/metabolismo , Restrição Calórica , Dieta com Restrição de Carboidratos/efeitos adversos , Carboidratos da Dieta/administração & dosagem , Ingestão de Energia/fisiologia , Humanos , Obesidade/epidemiologia , Obesidade/metabolismo , Resultado do Tratamento
13.
Interdiscip Top Gerontol ; 35: 39-68, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17063032

RESUMO

Elevated blood glucose associated with diabetes produces progressive and apparently irreversible damage to many cell types. Conversely, reduction of glucose extends life span in yeast, and dietary restriction reduces blood glucose. Therefore it has been hypothesized that cumulative toxic effects of glucose drive at least some aspects of the aging process and, conversely, that protective effects of dietary restriction are mediated by a reduction in exposure to glucose. The mechanisms mediating cumulative toxic effects of glucose are suggested by two general principles of metabolic processes, illustrated by the lac operon but also observed with glucose-induced gene expression. First, metabolites induce the machinery of their own metabolism. Second, induction of gene expression by metabolites can entail a form of molecular memory called hysteresis. When applied to glucose-regulated gene expression, these two principles suggest a mechanism whereby repetitive exposure to postprandial excursions of glucose leads to an age-related increase in glycolytic capacity (and reduction in beta-oxidation of free fatty acids), which in turn leads to an increased generation of oxidative damage and a decreased capacity to respond to oxidative damage, independent of metabolic rate. According to this mechanism, dietary restriction increases life span and reduces pathology by reducing exposure to glucose and therefore delaying the development of glucose-induced glycolytic capacity.


Assuntos
Envelhecimento/metabolismo , Glicemia/metabolismo , Restrição Calórica , Metabolismo Energético/genética , Privação de Alimentos , Óperon Lac , Longevidade/genética , Doenças Metabólicas/genética , Envelhecimento/fisiologia , Fatores de Transcrição de Zíper de Leucina e Hélice-Alça-Hélix Básicos , Glicemia/genética , Ingestão de Energia , Metabolismo Energético/fisiologia , Glicólise , Humanos , Longevidade/fisiologia , Doenças Metabólicas/fisiopatologia , Estresse Oxidativo
14.
Physiol Behav ; 85(1): 3-23, 2005 May 19.
Artigo em Inglês | MEDLINE | ID: mdl-15924903

RESUMO

Since nutrition-sensitive feedback signals normally act to maintain relatively stable levels of both available and stored nutritional resources, failure in one or more of these feedback signals could plausibly lead to obese phenotypes. The glucostatic hypothesis in its original form posited that glucose serves as a physiological satiety factor (in the sense that post-prandial increases in plasma glucose cause meal termination), but in this form the hypothesis has been difficult to prove, and, especially since the discovery of leptin, the glucostatic hypothesis has largely been abandoned. Nevertheless, reduction of plasma glucose levels or glucose signaling produces a profile of neuroendocrine responses similar to those produced by leptin deficiency. Since leptin is not a physiological satiety factor (because it does not increase before meal termination), yet leptin deficiency causes obesity, we suggest that the glucostatic hypothesis be re-formulated without reference to satiety (i.e., short-term effects on food intake). Instead we argue that like leptin signaling, glucose signaling regulates long-term energy balance, in part by regulating metabolic rate but also by chronically regulating food intake. We further speculate that high-fat diets produce obesity in part because carbohydrates are, per calorie, more effective than lipids to reduce food intake and increase metabolic rate. In support of this glucoadipostatic hypothesis, the 5 present review examines evidence that obesity and the metabolic syndrome may be due to reduction in neuroendocrine sensitivity to glucose leading to increased metabolic efficiency.


Assuntos
Metabolismo Energético , Glucose/metabolismo , Obesidade/etiologia , Transdução de Sinais , Adenilato Quinase/metabolismo , Animais , Glicemia/metabolismo , Dieta , Humanos , Hipotálamo/metabolismo , Hipotálamo/fisiopatologia , Insulina/metabolismo , Leptina/metabolismo , Obesidade/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Pró-Opiomelanocortina/fisiologia , Resposta de Saciedade/fisiologia , Termogênese/fisiologia
15.
Brain Res ; 993(1-2): 172-6, 2003 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-14642843

RESUMO

D-chiro-Inositol (DCI) enhances reproductive function in insulin-resistant women with polycystic ovarian disease and enhances the effects of insulin in the periphery, suggesting that this compound may act in part by sensitizing the hypothalamus to effects of insulin. Effects of gold-thioglucose (GTG) to produce hypothalamic lesions and subsequent obesity are insulin-dependent, suggesting that responses to GTG may be a marker of hypothalamic sensitivity to insulin. To assess these hypotheses, the present study assessed if DCI would enhance the ability of a subthreshhold dose of GTG to produce hypothalamic lesions and subsequent obesity. At the subthreshhold dose used (0.4 mg/kg i.p.), injection of GTG produced no subsequent effect on body weight compared to saline; similarly, at the dose of DCI used (10 mg/kg/day in drinking water), DCI produced no effect on body weight. In contrast, when given to mice exposed to DCI, this dose of GTG produced significant increase in body weight and evidence of an enhanced medial arcuate hypothalamic lesion.


Assuntos
Antirreumáticos/toxicidade , Aurotioglucose/toxicidade , Sinergismo Farmacológico , Hipotálamo/efeitos dos fármacos , Inositol/farmacologia , Proteína Relacionada com Agouti , Análise de Variância , Animais , Peso Corporal/efeitos dos fármacos , Hipotálamo/lesões , Hipotálamo/patologia , Peptídeos e Proteínas de Sinalização Intercelular , Masculino , Camundongos , Camundongos Endogâmicos , Pró-Opiomelanocortina/genética , Pró-Opiomelanocortina/metabolismo , Proteínas/metabolismo , RNA Mensageiro/metabolismo
16.
BMC Physiol ; 3: 5, 2003 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-12848900

RESUMO

BACKGROUND: Fasting and diabetes are characterized by elevated glucocorticoids and reduced insulin, leptin, elevated hypothalamic AGRP and NPY mRNA, and reduced hypothalamic POMC mRNA. Although leptin replacement can reverse changes in hypothalamic gene expression associated with fasting and diabetes, leptin also normalizes corticosterone; therefore the extent to which the elevated corticosterone contributes to the regulation of hypothalamic gene expression in fasting and diabetes remains unclear. To address if elevated corticosterone is necessary for hypothalamic responses to fasting and diabetes, we assessed the effects of adrenalectomy on hypothalamic gene expression in 48-hour-fasted or diabetic mice. To assess if elevated corticosterone is sufficient for the hypothalamic responses to fasting and diabetes, we assessed the effect of corticosterone pellets implanted for 48 hours on hypothalamic gene expression. RESULTS: Fasting and streptozotocin-induced diabetes elevated plasma glucocorticoid levels and reduced serum insulin and leptin levels. Adrenalectomy prevented the rise in plasma glucocorticoids associated with fasting and diabetes, but not the associated reductions in insulin or leptin. Adrenalectomy blocked the effects of fasting and diabetes on hypothalamic AGRP, NPY, and POMC expression. Conversely, corticosterone implants induced both AGRP and POMC mRNA (with a non-significant trend toward induction of NPY mRNA), accompanied by elevated insulin and leptin (with no change in food intake or body weight). CONCLUSION: These data suggest that elevated plasma corticosterone mediate some effects of fasting and diabetes on hypothalamic gene expression. Specifically, elevated plasma corticosterone is necessary for the induction of NPY mRNA with fasting and diabetes; since corticosterone implants only produced a non-significant trend in NPY mRNA, it remains uncertain if a rise in corticosterone may be sufficient to induce NPY mRNA. A rise in corticosterone is necessary to reduce hypothalamic POMC mRNA with fasting and diabetes, but not sufficient for the reduction of hypothalamic POMC mRNA. Finally, elevated plasma corticosterone is both necessary and sufficient for the induction of hypothalamic AGRP mRNA with fasting and diabetes.


Assuntos
Diabetes Mellitus Experimental/fisiopatologia , Jejum/fisiologia , Regulação da Expressão Gênica/fisiologia , Glucocorticoides/fisiologia , Hipotálamo/fisiologia , Hipotálamo/fisiopatologia , Medula Suprarrenal/fisiologia , Medula Suprarrenal/fisiopatologia , Medula Suprarrenal/cirurgia , Adrenalectomia/métodos , Proteína Relacionada com Agouti , Animais , Corticosterona/administração & dosagem , Corticosterona/farmacologia , Diabetes Mellitus Experimental/sangue , Implantes de Medicamento/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Glucocorticoides/sangue , Hipotálamo/efeitos dos fármacos , Insulina/deficiência , Insulina/genética , Insulina/fisiologia , Peptídeos e Proteínas de Sinalização Intercelular , Leptina/genética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Neuropeptídeo Y/genética , Pró-Opiomelanocortina/genética , Proteínas/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo
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