Breast cancer local recurrence after mastectomy with immediate latissimus dorsi myocutaneous flap reconstruction: A case report.

Even though most local recurrences after autologous breast reconstruction occur in superficial tissue, they also occur in deep tissue in the reconstructed breast. A 49-year-old woman presented with a bloody discharge from the right nipple. Ultrasonography revealed a hypoechoic area in her right breast, which was diagnosed as ductal carcinoma in situ on histopathology. We performed nipple-sparing mastectomy and immediate reconstruction of the breast with a latissimus dorsi myocutaneous flap. At 6 years postoperatively, the patient presented with a palpable mass. Ultrasonography revealed a solid mass lesion subcutaneously in the right breast. Computed tomography revealed multiple enhanced solid mass lesions in the subcutaneous and deep tissues of the reconstructed breast. The mass in the deep tissue of the reconstructed breast was diagnosed as an invasive micropapillary carcinoma by biopsy. For local recurrence, we performed wide excision of the reconstructed breast. The masses in the subcutaneous and deep tissues of the reconstructed breast were diagnosed as invasive micropapillary carcinoma. Superficial recurrence was first detected by physical examination, and deep recurrence was later detected with further imaging. We present a case of local recurrences that occurred in the deep tissue, in addition to superficial tissue of the reconstructed breast.

Surface modification of the cubic micro-cartilage by collagenase treatment and its efficacy in cartilage regeneration for ear tissue engineering.

BACKGROUND: In order to enhance cartilage regeneration, surface modification of the cubic micro-cartilage with the collagenase treatment was tested and its efficacy to tissue engineer ear cartilage was investigated. MATERIALS AND METHODS: Harvested cubic micro-cartilages were treated with collagenase with different digestion time (0, 15, 60, and 120 min). Histological, ultrastructural (SEM and TEM), and Western blot analyses were carried out. Subsequently, A total of 45 dogs were used to tissue engineer ear cartilage. Using collagenase-treated micro-cartilage, the ear cartilage regeneration with the prepared dilution (8, 12.5, 25, 50, 100%) of micro-cartilage block seeding was performed to determine the minimum amount of cartilage tissue required for ear tissue-engineering (n = 6 at each point in each group). At 10 weeks after surgery, samples were resected and subjected to histochemical and immune-histological evaluation for cartilage regeneration. RESULTS: In vitro study on micro-cartilage morphology and western blot analysis showed that collagenase digestion was optimal at 60 min for cartilage regeneration. In vivo evaluation on the reduced proportions of micro-cartilage block seeding onto implant scaffolds under 60-min collagenase digestion determined the minimum amount of cartilage tissue necessary to initiate a one-step ear cartilage regeneration in a canine autologous model, which was 12.5-25% of the original ear size. CONCLUSION: Tissue-engineering ear cartilage from limited volume of donor cartilage can possibly be achieved by the collagenase treatment on micro-cartilage to expand cartilage regeneration capacity, application of cytokine sustained-release system, and seeding on a suitable ear scaffold material.

A Comparative Study of Nail Fold Approach and Microscopic Nail Bed Approach in the Surgical Treatment of Subungual Exostosis.

ABSTRACT: Subungual exostosis is a benign tumor that occurs under the nail plate of the distal phalanx and causes nail deformity and pain. There are many reports of recurrence and postoperative nail deformity, and the surgical approach needs to be reassessed. Two typical surgical approaches are from the nail fold and the nail bed. Here, we compare 2 surgical approaches for subungual exostosis that were performed in our department. Twenty-three cases of subungual exostosis were treated between 2010 and 2019; 12 cases were treated by the nail fold approach and 11 by the microscopic nail bed approach. The nail fold approach resulted in not only scarring but also nail deformities, such as onychodystrophy. There were 2 (18.2%) cases of recurrence. In the microscopic nail bed approach, there was no case of recurrence. It was possible to preserve the thinned nail bed by dissecting under a microscope, and the thinned nail bed did not become necrotic in any cases. To solve postoperative nail deformity and a high recurrence rate, sufficient resection and meticulous surgical procedure on the nail bed and nail matrix are essential. The microscopic nail bed approach satisfied both of these criteria and was considered to be a useful method for subungual exostosis.

The Laterally Extended Paramedian Forehead Flap for Nasal Reconstruction: The Delay Technique Revisited.

BACKGROUND: Problems with poor circulation often occur when a large defect or a distant region, such as the apex of the nose, is covered with a paramedian forehead flap. Delay technique increases the safety of reconstruction procedures, but it has been used less frequently because a 2-stage surgery is necessary, and various other flaps and techniques have been developed. METHOD: We performed the delay technique of paramedian forehead flap at the same time as tumor resection. For the flap, a narrow pedicle of about 1-cm was prepared on the supratrochlear artery and vein, and the incision was extended toward the lateral side conforming to the defect morphology, and a paramedian forehead flap with a design consistent with the esthetic unit containing the defect was prepared. The region below the flap was dissected to create the flap bipedicle, and surgery was completed. RESULT: This procedure was used in 4 patients with malignant tumor of the external nose, and the flap survived perfectly in all patients. The postoperative esthetic outcome was also found to be good. CONCLUSIONS: This procedure does not increase the frequency of surgery, circulation in the flap is maintained, the flap pedicle on the supratrochlear artery can be made narrow, and flap thinning can be performed from the beginning. Coverage of an extensive defect is possible because a large flap can be excised, and satisfactory esthetic appearance can be obtained by matching with the esthetic unit. The delay technique for various flaps (not limited to forehead flap alone) should be considered an effective technique for the current treatment of malignant tumors.

Preliminary Study of PGA Fabric for Seromas at Latissimus Dorsi Flap Donor Sites.

BACKGROUND: Seroma formation is a major complication following latissimus dorsi (LD) flap transfer for breast reconstruction. We implanted a nonwoven polyglycolic acid (PGA) fabric-a biodegradable polymer-in the LD flap donor site and examined its effect on postoperative seroma formation and resolution in a comparative study on 38 patients undergoing primary 1-stage breast reconstruction by LD flap. METHOD: A PGA treatment group had a PGA fabric placed in the donor wound (PGA group, n = 20), whereas a second group was treated with standard donor site closure (control group, n = 18). The incidence of seromas was comparable between the groups. RESULT: There was a significant reduction in aspiration volume by needle aspiration after drain removal (P < 0.05) and in the time until seroma resolution (P < 0.01) in the PGA group compared with the control group. CONCLUSION: These results indicate that application of PGA at the LD donor site is useful in controlling postoperative seromas.

Evaluation of nanofiber-based polyglycolic acid scaffolds for improved chondrocyte retention and in vivo bioengineered cartilage regeneration.

BACKGROUND: In conventional studies on the regeneration of auricle-shaped cartilage in autogenous models using large animals, there were problems with the cartilage regeneration induction capacity and long-term retention of geometric shape. In this study, the authors sought to improve on outcome in these regards: a nonwoven fabric of polyglycolic acid was developed through nanotechnology, and the effect of nanofiber diameter on in vitro cell-seeding efficiency and the in vivo response after implantation in an autogenous large-animal model were evaluated. METHODS: Canine chondrocytes were isolated and seeded onto polyglycolic acid fabric ranging from 0.5 to 20 µm in average diameter. Cell seeding efficiency was highest for mid-range polyglycolic acid fibers (average diameter, 0.8, 3.0, and 7.0 µm). Flat and auricle-shaped scaffolds were constructed using polypropylene structural support, sandwiching a nonwoven polyglycolic acid fabric that contained autogenous chondrocytes together with basic fibroblast growth factor-laden particles and an exterior fibrin sealant. Scaffolds were then implanted autogenously and evaluated at 5 and 20 weeks. RESULTS: Biomechanical strength was optimal for polyglycolic acid fiber diameters of 0.8 to 3.0 µm. Optimal cell maintenance and neocartilage response were seen with polyglycolic acid fiber diameters in the same mid-range for nanofiber constructs. CONCLUSION: These findings demonstrate the potential for nanoscale modulation of auricle-shaped cartilage regeneration in a large-animal model.

What is the best strategy for Asians with involutional entropion?

BACKGROUND: Even though many different procedures have been proposed to involutional entropion, there is no established criterion standard in terms of the choice of the operative method. Considering the anatomical difference of the lower eyelid structure between Asians and whites, we evaluated the effectiveness of our surgical approach to involutional entropion exclusively for Japanese patients. METHODS: Twenty-one patients complained of discomfort around the eye, and they also suffered from foreign body sensation, ocular pain, epiphora, and photophobia. All the patients with the involutional entropion were surgically treated with combined procedures (the lower eyelid retractors advancement, the modified Hotz method, and the modified Wheeler method). RESULTS: Twenty-three lower eyelids of 21 patients with involutional entropion underwent surgery. There were 10 men and 11 women with a mean age of 78 years (range, 69-94 years). All patients we operated on were completely satisfied with any residual ocular symptoms. There was no case of recurrence following the primary procedure during 16 months of the mean follow-up period. CONCLUSION: Based on the anatomical difference in various ethnic groups, we propose the best strategy for Asian patients with the involutional entropion.

Long-term result of a biodegradable osteo-inductive copolymer for the treatment of orbital blowout fracture.

PURPOSE: We have recently reconstructed the orbital wall using a bioabsorbable osteo-inductive copolymer as a support material for the bone defects in patients with orbital blowout fracture. The purpose of this study was to investigate a 2-year follow-up after implantation. MATERIAL AND METHODS: In order to investigate the efficacy and safety of the bioabsorbable osteo-inductive copolymer HA-P(LA/CL), hydroxyapatite-poly(l-lactide-ε-caprolactone), we measured CT density of the implanted copolymers in 7 patients at such time points as 1 week, 6 months and 2 years after surgery along with the long-term outcomes with regard to postoperative complications. RESULTS: Five patients in whom the intraoperative maximum width of the bone defect was less than 15 mm demonstrated the fracture types of either linear or trap-door. All patients treated with HA-P(LA/CL) copolymer indicated good bone formation with high CT density (>500) at 2 years postoperatively. In contrast, 2 patients in whom the intraoperative maximum width of the bone defect was more than 16 mm demonstrated a punched-out type fracture. Postoperative CT of the HA-P(LA/CL) copolymer showed low density (28.0 and 173.4) at 2 years postoperatively, indicative of limited bone formation. None of the cases demonstrated significant postoperative complications such as diplopia, enophthalmos, infection and extrusion of the implant material. CONCLUSION: The results of this study demonstrate that surgical treatment of orbital blowout fractures using bioabsorbable osteo-inductive copolymer was useful in linear and trap-door fractures.

Usefulness of polyglycolic acid-polypropylene composite scaffolds for three-dimensional cartilage regeneration in a large-animal autograft model.

BACKGROUND: Approaches to auricular reconstruction have shown improved outcome when a basic fibroblast growth factor (bFGF) slow-release system and fibrin spraying are combined with biodegradable polymers. More complex, three-dimensional structures, such as those that replicate the human auricle, are often lost because of biodegradation of the synthetic scaffold. METHODS: To improve the mechanical strength of regenerated cartilage, the authors grafted canine autologous chondrocytes after seeding onto scaffolds made of a complex of polyglycolic acid and polypropylene, incorporating a slow-release bFGF system with a fibrin spray coating. RESULTS: Five weeks after grafting, thicker cartilage with increased bending stress was obtained with the slow-release bFGF. In a three-polyglycolic acid-layer construct sandwiched around polypropylene, simulating a three-dimensional auricular structure, greater cartilage regeneration and angiogenesis were found around the implant. Sox5-positive cells were identified, indicative of maturation of neocartilage with chondroblast proliferation. CONCLUSION: These results support the usefulness of combining absorbable and nonabsorbable materials (polyglycolic acid and polypropylene) in composite scaffolds for autologous cartilage regeneration in a large-animal autograft model.

Mozart ear: diagnosis, treatment, and literature review.

Mozart ear is a congenital auricular deformity, which is mainly characterized by a bulging appearance of the anterosuperior portion of the auricle, a convexly protruded cavum conchae, and a slit-like narrowing of the orifice of the external auditory meatus. It is said to be uncommon, and because no one has yet fully described neither the disease nor the treatment, the concept of Mozart ear has not been unified. This report describes a case of a 13-year-old girl presented with an unusual congenital deformity which showed the features of Mozart ear. It is an extremely rare deformity that only about 4 clinical cases have been reported in medical literature thereby a treatment method has not been fully discussed. For surgical correction of our cases, we excised deformed conchal cartilage, turned it over, regrafted, and maintained a cosmetically positive result. We also reviewed and described the origin, current concept, and treatment method of Mozart ear.

Randomized controlled trial of the application of topical b-FGF-impregnated gelatin microspheres to improve tissue survival in subzone II fingertip amputations.

We undertook a randomized controlled trial of subzone II fingertip amputations, comparing standard treatment with topical application of gelatin microspheres prepared with basic fibroblast growth factor (b-FGF) to provide a slow, sustained release of b-FGF with microsphere degradation. Forty-eight digits from 42 patients were randomized into the two study arms. The microspheres were applied as a paste on exposed tissue surfaces, whereas standard treatment was without any topical treatment. Patients were treated either with microsurgical revascularization or by simple composite graft, based on the surgeon's clinical decision. Tissue survival of the replanted fingertips was measured by a blinded evaluator 3 weeks postoperatively. A modest improvement in survival was seen with b-FGF-microsphere application for both revascularized and composite grafted fingertips, though this did not achieve statistical significance. Whether the slow release of b-FGF through a bioresorbable carrier gives any improvement in outcome in patients with subzone II fingertip amputations is unproven.