RESUMO
Retinal horizontal cells (HCs) are inhibitory neurons, which modulate the transmission of light-elicited signals from photoreceptors to bipolar cells in the outer retina. HCs of the same physiological type are extensively coupled via gap junctions. In the zebrafish retina, the population of HCs comprises up to four morphologically distinct subtypes. Four different connexins (Cx52.6, Cx52.7, Cx52.9 and Cx55.5) were detected in these cells with overlapping expression patterns. In this study, we show that Cx52.6 is alternatively spliced in the retina, resulting in an additional isoform, designated as Cx53.4, which differs from the originally described Cx52.6 only by the final C-terminal peptide (12 vs. 4 aa). Further protein sequence alignments revealed that Cx53.4 represents the counterpart of alternatively spliced mouse Cx57 and human Cx62. RT-PCR analyses of mRNA expression in different adult zebrafish tissues showed that Cx53.4 is expressed exclusively in the retina. The localization of Cx53.4 protein within the retina was analyzed using a specific antibody. Immunofluorescence analyses demonstrated that the expression of Cx53.4 is restricted to HCs of all four subtypes. Further, immunoelectron microscopy confirmed the presence of Cx53.4 in gap junctions between HC dendrites and between their axon terminals.
Assuntos
Conexinas/metabolismo , Células Horizontais da Retina/metabolismo , Proteínas de Peixe-Zebra/metabolismo , Peixe-Zebra/metabolismo , Processamento Alternativo , Animais , Animais Geneticamente Modificados , Axônios/metabolismo , Células Cultivadas , Conexinas/genética , Dendritos/metabolismo , Junções Comunicantes/metabolismo , Isoformas de Proteínas/metabolismo , RNA Mensageiro/metabolismo , Células Horizontais da Retina/citologia , Alinhamento de Sequência , Peixe-Zebra/anatomia & histologia , Proteínas de Peixe-Zebra/genéticaRESUMO
In the vertebrate retina, horizontal cells (HCs) reveal homologous coupling by gap junctions (gj), which are thought to consist of different connexins (Cx). However, recent studies in mouse, rabbit and zebrafish retina indicate that individual HCs express more than one connexin. To provide further insights into the composition of gj connecting HCs and to determine whether HCs express multiple connexins, we examined the molecular identity and distribution of gj between HCs of the carp retina. We have cloned four carp connexins designated Cx49.5, Cx55.5, Cx52.6 and Cx53.8 with a close relationship to connexins previously reported in HCs of mouse, rabbit and zebrafish, respectively. Using in situ hybridization, Cx49.5 expression was detected in different subpopulations of retinal neurons including HCs, whereas the Cx52.6 transcript was localized exclusively in HCs. Using specific antibodies, Cx55.5 and Cx53.8 were detected on dendrites of all four HC subtypes and axon terminals. Immunoelectron microscopy confirmed the presence of Cx55.5 and Cx53.8 in gap junctions between these processes and Cx55.5 was additionally observed in HC dendrites invaginating cone pedicles, suggesting its participation in the modulation of photoreceptor output in the carp retina. Furthermore, using single-cell RT-PCR, all four connexins were detected in different subtypes of HCs, suggesting overlapping expression patterns. Thus, the composition of gj mediating homologous coupling between subtypes of carp HCs appears to be more complex than expected. Moreover, BLAST searches of the preliminary carp genome, using novel sequences as query, suggest that most of the analyzed connexin genes are duplicated in carp.
Assuntos
Carpas/anatomia & histologia , Carpas/metabolismo , Junções Comunicantes/metabolismo , Células Horizontais da Retina/citologia , Células Horizontais da Retina/metabolismo , Sequência de Aminoácidos , Animais , Axônios/metabolismo , Western Blotting , Linhagem Celular Tumoral , Conexinas/metabolismo , Dendritos/metabolismo , Proteínas de Peixes/metabolismo , Imuno-Histoquímica , Hibridização In Situ , Camundongos , Microscopia Imunoeletrônica , Reação em Cadeia da Polimerase , Isoformas de Proteínas , Alinhamento de SequênciaRESUMO
The subunit composition of ionotropic glutamate receptors (GluRs) is extremely diverse and responsible for the diversity of postsynaptic responses to the release of glutamate, which is the major excitatory neurotransmitter in the retina. To understand the functional consequences of this diversity, it is necessary to reveal the synaptic localization and subunit composition of GluRs. We have used immuno light and electron microscopy to localize AMPA and kainate (GluR1, GluR2/3, GluR4, GluR5-7) subunits in identified carp retinal neurons contributing to the outer plexiform layer. GluR1 could not be detected within the outer plexiform layer. Rod and cone horizontal cells all express only GluR2/3 at the tips of their invaginating dendrites. These receptors are also inserted into the membrane of spinules, light-dependent protrusions of the horizontal cell dendrites, flanking the synaptic ribbon of the cone synapse. Bipolar cells express GluR2/3, GluR4, and GluR5-7 at their terminal dendrites invaginating cone pedicles and rod spherules. Colocalization data suggest that each subunit is expressed by a distinct bipolar cell type. The majority of bipolar cells expressing these receptors seem to be of the functional OFF-type; however, in a few instances, GluR2/3 could also be detected on dendrites of bipolar cells that, based on their localization within the cone synaptic complex, appeared to be of the functional ON-type. The spatial arrangement of the different subunits within the cavity of the cone pedicle appeared not to be random: GluR2/3 was found predominantly at the apex of the cavity, GluR4 at its base and GluR5-7 dispersed between the two.
Assuntos
Carpas/fisiologia , Receptores de AMPA/metabolismo , Receptores de Ácido Caínico/metabolismo , Retina/anatomia & histologia , Retina/metabolismo , Sinapses/metabolismo , Animais , Western Blotting , Microscopia Confocal , Microscopia de Fluorescência , Microscopia Imunoeletrônica , Testes de Precipitina , Receptores de AMPA/ultraestrutura , Receptores de Ácido Caínico/ultraestrutura , Retina/ultraestrutura , Células Fotorreceptoras Retinianas Cones/metabolismo , Células Fotorreceptoras Retinianas Cones/ultraestrutura , Células Fotorreceptoras Retinianas Bastonetes/metabolismo , Células Fotorreceptoras Retinianas Bastonetes/ultraestrutura , Sinapses/ultraestruturaRESUMO
Connexin26 (Cx26) is a member of the family of integral membrane proteins that normally form intercellular gap junctional channels. We have used Western blotting, immunofluorescence, immunoelectron microscopy, and single-cell reverse-transcriptase polymerase chain reaction amplification (RT-PCR) to analyze the expression and cellular localization of Cx26 in the carp retina. In the outer plexiform layer, strong clustered Cx26 immunolabeling was concentrated at and restricted to the terminal dendrites of horizontal cells. Single-cell RT-PCR confirmed the expression of Cx26 in carp retinal horizontal cells. 248-bp fragments amplified from cDNAs of four different horizontal cells were cloned and each nucleotide sequence encodes a protein fragment (AA 104-185) with highly significant homology to rat and mouse Cx26. Immunoelectron microscopy revealed that only the invaginating dendrites of horizontal cells in intimate lateral association with the presynaptic ribbon complex were labeled. No labeling was found at the photoreceptor membrane and there was no septalaminar structure, indicative of gap junctions, between photoreceptors and horizontal cells. The focal location of Cx26 at the membrane of the dendritic tips of horizontal cells and the lack of gap junctional morphology suggests that Cx26 might form hemichannels.
Assuntos
Carpas , Conexinas/análise , Neurônios/química , Células Fotorreceptoras de Vertebrados/química , Sequência de Aminoácidos , Animais , Western Blotting , Conexina 26 , Dendritos/química , Técnica Indireta de Fluorescência para Anticorpo , Junções Comunicantes/química , Microscopia de Fluorescência , Microscopia Imunoeletrônica , Dados de Sequência Molecular , Neurônios/citologia , Neurônios/ultraestrutura , Células Fotorreceptoras de Vertebrados/citologia , Células Fotorreceptoras de Vertebrados/ultraestrutura , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Homologia de Sequência de AminoácidosRESUMO
An essential feature of the first synapse in the retina is a negative feedback pathway from horizontal cells to cones. Here we show that at this synapse, connexin26 forms hemichannels on horizontal cell dendrites near the glutamate release site of the cones. Blocking these hemichannels hyperpolarizes horizontal cells, modulates the Ca2+ channels of the cones, and abolishes all feedback-mediated responses. We propose a feedback mechanism in which the activity of the Ca2+ channels and the subsequent glutamate release of the cones are modulated by a current through these hemichannels. Because the current through the hemichannels depends on the polarization of the horizontal cells, their activity modulates the output of the cones.
Assuntos
Carpas/fisiologia , Conexinas/metabolismo , Retina/metabolismo , Animais , Cálcio/metabolismo , Canais de Cálcio/metabolismo , Carbenoxolona/farmacologia , Conexina 26 , Conexinas/antagonistas & inibidores , Conexinas/química , Dendritos/efeitos dos fármacos , Dendritos/metabolismo , Condutividade Elétrica , Agonistas de Aminoácidos Excitatórios/farmacologia , Retroalimentação , Ácido Glutâmico/metabolismo , Ácido Glutâmico/farmacologia , Imuno-Histoquímica , Ativação do Canal Iônico/efeitos dos fármacos , Ácido Caínico/farmacologia , Luz , Potenciais da Membrana/efeitos dos fármacos , Modelos Neurológicos , Retina/citologia , Retina/efeitos dos fármacos , Células Fotorreceptoras Retinianas Cones/citologia , Células Fotorreceptoras Retinianas Cones/efeitos dos fármacos , Células Fotorreceptoras Retinianas Cones/metabolismo , Sinapses/efeitos dos fármacos , Sinapses/metabolismoRESUMO
Electrical synapses (gap junctions) in neuronal circuits have become a major focus in the study of network properties such as synchronization and oscillation (Galarreta and Hestrin, 1999; Gibson et al., 1999). Despite the recent progress made in unraveling the contribution of gap junctions to network behavior, little is known about the molecular composition of the junctional constituents. By cloning gap junction proteins [connexins (Cxs)] from zebrafish retina and through functional expression, we demonstrate that the retina possesses a high degree of connexin diversity, which may account for differential functional properties of electrical synapses. Three new Cxs, designated as zebrafish Cx27.5 (zfCx27.5), zfCx44.1, and zfCx55.5, and the carp ortholog of mammalian Cx43 were cloned. By in situ hybridization and in situ RT-PCR, we demonstrate that the four fish connexin mRNAs show differential localization in the retina. Transient functional expression in paired Xenopus oocytes and in the neuroblastoma N2A cell line indicate an extreme range of electrophysiological properties of these connexins in terms of voltage dependence and unitary conductance. For instance, the new zfCx44.1 exhibited high sensitivity to voltage-induced closure with currents decaying rapidly for transjunctional potentials >10 mV, whereas zfCx55.5 channels showed an opposite voltage dependence in response to voltage steps of either polarity. Moreover, although zfCx44.1 channels showed unitary conductance as high as any previously reported for junctional channels (nearly 300 pS), zfCx55. 5 and zfCx27.5 exhibited much lower unitary conductances (<60 pS).
Assuntos
Conexina 43/genética , Conexinas/genética , Proteínas do Olho/genética , Retina/metabolismo , Proteínas de Peixe-Zebra , Animais , Carpas , Células Cultivadas , Clonagem Molecular , Conexina 26 , Conexina 43/metabolismo , Conexinas/metabolismo , Sequência Conservada , Proteínas do Olho/metabolismo , Feminino , Junções Comunicantes/metabolismo , Expressão Gênica , Hibridização In Situ , Microinjeções , Dados de Sequência Molecular , Neurônios/citologia , Neurônios/metabolismo , Oócitos/citologia , Oócitos/metabolismo , Especificidade de Órgãos/genética , Técnicas de Patch-Clamp , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Alinhamento de Sequência , Sinapses/metabolismo , Xenopus laevis , Peixe-ZebraRESUMO
Ambient light conditions affect the morphology of synaptic elements within the cone pedicle and modulate the spatial properties of the horizontal cell receptive field. We describe here that the effects of retinoic acid on these properties are similar to those of light adaptation. Intraorbital injection of retinoic acid into eyes of dark-adapted carp that subsequently were kept in complete darkness results in the formation of numerous spinules at the terminal dendrites of horizontal cells, a typical feature of light-adapted retinae. The formation of these spinules during light adaptation is impaired in the presence of citral, a competitive inhibitor of the dehydrogenase responsible for the generation of retinoic acid in vivo. Intracellularly recorded responses of horizontal cells from dark-adapted eyecup preparations superfused with retinoic acid reveal typical light-adapted spatial properties. Retinoic acid thus appears to act as a light-signaling modulator. Its activity appears not to be at the transcriptional level because its action was not blocked by actinomycin.
Assuntos
Adaptação Ocular/efeitos dos fármacos , Adaptação Ocular/fisiologia , Ceratolíticos/administração & dosagem , Retina/fisiologia , Tretinoína/administração & dosagem , Animais , Carpas , Neurônios/citologia , Neurônios/efeitos dos fármacos , Retina/citologiaRESUMO
The distribution of Connexin43 (Cx43) was examined by immunoblotting and immunofluorescence microscopy in the retinas of five different vertebrates by using a C-terminal specific peptide antibody. The specificity of the antibody was proved on immunoblots, in which it showed cross reactivity with a 43-kDa protein in rat heart homogenates as well as in homogenates of rabbit, rat, chicken, turtle, and fish (carp and zebrafish) retinas. Immunofluorescence histochemistry with retinal cryosections revealed the presence of Cx43 in the retinal pigment epithelium cells of all tested species and in blood vessels of vascularized retinas (fish and rat). Cx43 immunoreactivity was further localized in the stria medullaris of rabbit retina, in the nerve fiber layer of rat retina, most likely in astrocytes, and in the area of the outer limiting membrane of the fish retina, most likely representing Cx43 in Müller glia cells. A punctate Cx43-immunoreactive pattern consistent with gap junctions was also detected in the outer plexiform layer of carp and zebrafish retinas, and a specific amacrine cell type, which ramified in two layers of the inner plexiform layer, was labeled in the zebrafish retina. The present results are in accordance with previous findings showing the abundance of Cx43 in astrocytes, endothelium, and epithelial cells. However, the presence of Cx43 immunoreactivity in a specific population of amacrine cells of the zebrafish retina might indicate that a Cx43-like protein is also expressed in neurons.
Assuntos
Conexina 43/análise , Retina/citologia , Vertebrados/anatomia & histologia , Animais , Especificidade de Anticorpos , Carpas , Galinhas , Feminino , Imunofluorescência , Immunoblotting , Imuno-Histoquímica , Coelhos , Ratos , Ratos Sprague-Dawley , Tartarugas , Peixe-ZebraRESUMO
Calcium is involved in many aspects of synaptic plasticity and we have analyzed its involvement in spinule dynamics at retinal horizontal cell dendrites. We show here that in particular the retraction of spinules is a Ca(2+)-dependent process. Inhibiting calmodulin or CaMKII, blocked the retraction that was also impaired in low calcium Ringer. Changes of the cytosolic Ca(2+)-concentration through depletion of internal Ca(2+)-stores were without effect. This suggested that Ca(2+)-influx during dark adaption and subsequent activation of CaMKII is an important step for spinule retraction. Voltage dependent Ca(2+)-channels were not responsible for the Ca(2+)-influx, rather Ca2+ leaking through alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)/kainate-gated channels. This suggested a close local link between AMPA/kainate receptors and CaMKII indicating a possible postsynaptic function of spinules. The distribution of bound, omega-shaped vesicles within the cone pedicles and its dependence on artificial depolarization further supported the idea of a postsynaptic function of spinules.
Assuntos
Cálcio/fisiologia , Carpas/metabolismo , Dendritos/metabolismo , Retina/metabolismo , Sinapses/metabolismo , Adaptação Ocular/fisiologia , Animais , Calmodulina/antagonistas & inibidores , Técnicas de Cultura , Microscopia Eletrônica , Plasticidade Neuronal/fisiologia , Fosforilação , Retina/ultraestrutura , Células Fotorreceptoras Retinianas Cones/ultraestruturaRESUMO
Optokinetic and phototactic behaviors of zebrafish larvae were examined for their usefulness in screening for recessive defects in the visual system. The optokinetic response can be reliably and rapidly detected in 5-day larvae, whereas the phototactic response of larvae is variable and not robust enough to be useful for screening. We therefore measured optokinetic responses of mutagenized larvae as a genetic screen for visual system defects. Third-generation larvae, representing 266 mutagenized genomes, were examined for abnormal optokinetic responses. Eighteen optokinetic-defective mutants were identified and two mutants that did not show obvious morphological defects, no optokinetic response a (noa) and partial optokinetic response a (poa), were studied further. We recorded the electroretinogram (ERG) to determine whether these two mutations affect the retina. The b-wave of noa larvae was grossly abnormal, being delayed in onset and significantly reduced in amplitude. In contrast, the ERG waveform of poa larvae was normal, although the b-wave was reduced in amplitude in bright light. Histologically, the retinas of noa and poa larvae appeared normal. We conclude that noa larvae have a functional defect in the outer retina, whereas the outer retina of poa larvae is likely to be normal.
Assuntos
Anormalidades do Olho/genética , Mutação , Seleção Genética , Peixe-Zebra/genética , Animais , Comportamento Animal , Cruzamentos Genéticos , Eletrorretinografia , Etilnitrosoureia/farmacologia , Olho/anatomia & histologia , Genes Recessivos , Larva , Luz , Mutagênese , Nistagmo Optocinético , Nervo Óptico/anatomia & histologia , Retina/anormalidadesRESUMO
The formation of spinules at the terminal dendrites of retinal horizontal cells with the onset of light and their subsequent retraction during darkness is a remarkable example of synaptic plasticity where sensory experience modifies reversibly, and on a time scale of minutes the ultrastructure of synaptic connectivity. The signals and the subsequent intracellular cascades underlying the prominent morphological alterations are only partially understood. We show here that lowering the external calcium concentration did prevent dark- and AMPA-induced retraction of spinules in a eyecup preparation. Furthermore, spinule retraction was prevented in vivo by the injection of calmidazolium, an inhibitor of calmodulin, into the eyeball, and also by the injection of KN-62, an inhibitor of Ca2+/calmodulin-dependent protein kinase (CaMkII). We conclude that local Ca2+ influx through AMPA-gated channels followed by activation of CaMkII is an important step for spinule retraction during dark adaptation. The phosphorylation patterns of phosphoproteins derived from purified horizontal cells was affected by the inhibitors of calmodulin and CaMkII respectively. Some of the affected phosphoproteins appeared to be cytoskeleton-associated proteins, including GAP-43. Based on these observations, a putative scenario for the retraction of spinules is proposed.
Assuntos
Proteínas Quinases Dependentes de Cálcio-Calmodulina/metabolismo , Carpas/fisiologia , Neuritos/fisiologia , Retina/fisiologia , Animais , Proteínas Quinases Dependentes de Cálcio-Calmodulina/antagonistas & inibidores , Ativação Enzimática/efeitos dos fármacos , Ativação Enzimática/fisiologia , Inibidores Enzimáticos/farmacologia , Proteína GAP-43 , Imidazóis/farmacologia , Glicoproteínas de Membrana/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Neuritos/enzimologia , Neuritos/ultraestrutura , Proteínas de Neurofilamentos/metabolismo , Fosforilação , Terminações Pré-Sinápticas/fisiologia , Retina/enzimologia , Retina/ultraestruturaRESUMO
The injection of phorbol esters into the eyes of dark-adapted teleost fish can mimic light effects in the retina and induces corresponding synaptic plasticity of horizontal cells (HCs). It is therefore very likely that protein kinase C (PKC) mediates light-induced synaptic plasticity. In the present study, we investigated the distribution of PKC, the phorbol ester receptor, in isolated HCs and in the whole retina by using tritated phorbol 12,13-dibutyrate ([3H]PDBu). The binding characteristics analyzed for HC homogenates and retinal homogenates revealed that [3H]PDBu binding is time dependent, specific, saturable, and reversible. Binding sites in HCs displayed a dissociation constant of 11.5 nM and a total number of 2.8 pmol/mg of protein. Autoradiography revealed that [3H]PDBu labeling is present in all retinal layers, including HCs, where it is associated with the somata. Furthermore, the treatment with PDBu strongly affected the endogenous phosphorylation of several membrane, cytosolic, and HC proteins and led to PKC activation as measured by H1 histone phosphorylation. In HCs, the treatment with PDBu in particular affected the amount of 32P incorporated into a group of phosphoproteins (68, 56/58, 47, 28, and 15 kDa) that were recently shown to be affected by light adaptation. These proteins might therefore be considered as important components of the observed morphological and physiological synaptic plasticity of HCs in the course of light adaptation.
Assuntos
Carpas/metabolismo , Dibutirato de 12,13-Forbol/metabolismo , Dibutirato de 12,13-Forbol/farmacologia , Proteína Quinase C/metabolismo , Retina/efeitos dos fármacos , Retina/metabolismo , Animais , Autorradiografia , Sítios de Ligação , Ativação Enzimática , Proteínas do Olho/metabolismo , Fosforilação , Retina/citologia , Fatores de TempoRESUMO
The contribution of ionotropic and metabotropic glutamate receptors to inositol polyphosphate accumulation in carp retinal slices was investigated using myo-[2-3H]inositol prelabelling. In the presence of the glutamate agonists quisqualate, (RS)-alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) and trans-(+/-)-1-amino-1,3-cyclopentane-dicarboxylic acid (t-ACPD), formation of [3H]inositol phosphate was significantly increased in a dose-dependent manner, with EC50 values of 350 nM, 1.5 microM and 10 microM respectively. The complete AMPA-induced response and a large component of the quisqualate-induced response were inhibited in a competitive manner when the ionotropic antagonist 6-cyano-7-nitroquinoxalin- 2,3-dione (CNQX) was present. Furthermore, the remaining level of quisqualate-induced [3H]inositol phosphate formation closely matched that produced by ACPD alone, and coincubation of AMPA and ACPD showed additive effects, suggesting that the quisqualate-induced response resulted from coactivation of metabotropic and ionotropic glutamate receptors. The ionotropic component was partially reduced in the presence of cobalt, suggesting indirect effects resulting from synaptic interactions. We could exclude indirect effects through depolarization-induced release of other neurotransmitters. Only serotonin (EC50 1 microM) and carbachol (at a concentration of 1 mM) stimulated [3H]inositol phosphate formation, but their antagonists did not affect the quisqualate response and coactivation with quisqualate and serotonin or carbachol resulted in additive effects. The ionotropic component was completely suppressed when Ca2+ was omitted from the medium and cobalt was present. This makes it likely that the ionotropic component resulted from Ca2+ entry through AMPA-gated channels and subsequent Ca(2+)-dependent activation of phospholipase C.
Assuntos
Carpas/metabolismo , Fosfatos de Inositol/metabolismo , Receptores de Glutamato/metabolismo , Receptores de Glutamato Metabotrópico/metabolismo , Retina/metabolismo , Animais , Colinérgicos/farmacologia , Cobalto/farmacologia , Adaptação à Escuridão/fisiologia , Agonistas de Dopamina/farmacologia , Agonistas de Aminoácidos Excitatórios/farmacologia , Técnicas In Vitro , Receptores de Glutamato Metabotrópico/agonistas , Retina/efeitos dos fármacos , Agonistas do Receptor de Serotonina/farmacologiaRESUMO
A substance immunoreactive to antibodies directed against bovine neurotensin (NT) was localized in neurons in the supraesophageal ganglion (SEG) and optic lobes of larval and adult Locusta migratoria L. Two large somata were located in the caudal cortex, ventral to the calyces and symmetrical to the median of the SEG. Four smaller somata also in the caudal cortex were located as two symmetrical pairs at the level of the central body. These somata formed a diffuse network of varicose fibers from the superior lateral to the ventro-lateral protocerebrum between the pedunculi and frontal cortical region. Some fibers crossed the median to the contralateral sides of the SEG. Another pair of immunoreactive somata whose terminating processes remained unclear was found at the level of the antennal lobes. Intrinsic networks of fibers were labeled in the accessory medulla and in layer 4/5 of the medulla. These fibers originated from 8-10 small somata near the dorso-frontal rim of the medulla. All larval stages contained these NT-like immunoreactive structures. Results from isoelectric focusing and press-blot analysis of SEG homogenates, synthetic neurotensin and neurotensin fragments indicate that this substance is similar to bovine neurotensin(1-13).
Assuntos
Química Encefálica/fisiologia , Gânglios dos Invertebrados/metabolismo , Gafanhotos/metabolismo , Neurotensina/metabolismo , Animais , Especificidade de Anticorpos , Encéfalo/anatomia & histologia , Bovinos , Feminino , Gânglios dos Invertebrados/anatomia & histologia , Gânglios dos Invertebrados/imunologia , Immunoblotting , Imuno-Histoquímica , Focalização Isoelétrica , Masculino , Bulbo/anatomia & histologia , Bulbo/fisiologia , Neurônios/metabolismo , Neurotensina/imunologiaRESUMO
Horizontal cells, which are second-order neurons of the vertebrate retina, exhibit synaptic plasticity governed by light and dark adaptation. We have investigated the alterations in the protein phosphorylation patterns of isolated carp (Cyprinus carpio) horizontal cells in relation to their state of light adaptation by using an in vitro phosphorylation assay and compared the resulting data with protein synthesis patterns of the whole retina. Phosphoproteins and [35S]methionine-labelled proteins were analysed by one- and two-dimensional gel electrophoresis followed by autoradiography. The state of light adaptation significantly affected the in vitro phosphorylation of horizontal cell proteins with molecular weights of 68, 56/58, 47, 28 and 15 kDa, but had no effect on the protein synthesis of retinal proteins. In the light the most prominent increase of 32P incorporation was observed in the 47 kDa protein. The biochemical properties of this protein closely resembled those of the growth-associated GAP-48, found in the fish retina. In addition, the phosphorylation of horizontal cell homogenates in the presence of protein kinase activators such as cyclic AMP, calcium, calmodulin and phospholipids revealed that horizontal cells of the fish retina contain cyclic AMP-, calcium/calmodulin- and calcium/phospholipid-dependent protein kinase activity resulting in the phosphorylation of several horizontal cell proteins, including the phosphoproteins which were affected by the state of light adaptation.
Assuntos
Adaptação Fisiológica , Luz , Retina/metabolismo , Animais , Autorradiografia , Cálcio/fisiologia , Calmodulina/fisiologia , Carpas , AMP Cíclico/fisiologia , Adaptação à Escuridão , Eletroforese em Gel de Poliacrilamida , Proteínas do Olho/biossíntese , Fosfolipídeos/farmacologia , Fosforilação , Proteína Quinase C/farmacologia , Retina/citologiaRESUMO
Dendrites of horizontal cells in the carp retina which invaginate the cone pedicles form numerous spinules during light adaptation. We have analyzed the contribution of cytoskeletal elements to this process. Isolated horizontal cells and frozen sections were screened with phalloidin for the existence of F-actin. F-actin was present in all types of horizontal cells and particularly enriched in the distal parts of the dendrites. Electron microscopical analysis demonstrated that interruption of the F-actin polymerization with cytochalasin B inhibited the formation of spinules during light adaptation. The persistence of spinules was also affected. Cytochalasin B also prevented the light-independent, phorbol ester-induced formation of spinules. Cytochalasin B only affected the morphology of the lateral, spinule-forming dendrites of cone horizontal cells within the cone pedicles, leaving the central, non spinule-forming dendrites of cone horizontal cells and the processes of rod horizontal cells within rod spherules unaffected. Whereas cytochalasin B prevented the protrusion of spinules, the spinule-associated membrane densities were only slightly affected. The two main characteristics of spinules, protrusion and membrane densities are therefore independently regulated processes.