An investigation into the influence of fermented cottonseed meal on the productive performance, egg quality, and gut health in laying hens.

The present study investigates the effects of replacing soybean meal (SBM) with either cottonseed meal (CSM) or fermented cottonseed meal (FCSM) on the productive performance, egg quality, blood biochemistry parameters, gut bacterial population, and small intestinal morphology of laying hens. A total of 648 Hy-Line W36 laying hens aged 40 weeks were randomly assigned to 9 treatments, with 6 replicates each and 12 birds per replicate. The feeding trial lasted 12 weeks. The treatments consisted of a control diet based on corn and SBM, as well as 8 experimental diets in which 7.5, 15, 22.5, and 30% of the SBM in the control diet was replaced with either CSM or FCSM. Laying hens fed diets with different levels of FCSM had higher egg production and egg mass than those fed with CSM diets at weeks 46 to 51 (P < 0.05). Diets containing FCSM also significantly improved the feed conversion ratio at weeks 40 to 45 and 46 to 51 (P < 0.05). Eggshell strength was significantly greater in birds fed diets containing FCSM than those fed other dietary treatments at 51 weeks of age (P < 0.05). Hens fed diets containing FCSM had higher calcium and lower cholesterol in serum than those on other diets (P < 0.05). Replacing SBM with FCSM decreased the egg yolk cholesterol content (P < 0.05). Additionally, feeding diets containing different levels of FCSM increased villus height and villus height to crypt depth in the jejunum (P < 0.05). Diets containing FCSM also reduced pH and coliform population in the ileum, and ceca and increased lactic acid bacteria count in the crop and ceca (P < 0.05). Overall, the present data showed that including FCSM in the diet of laying hens can positively affect productive performance compared to CSM. Moreover, substituting SBM with FCSM, can improve eggshell quality, promote gut health, and reduce egg yolk cholesterol concentration.

Effects of black pepper and turmeric powder on growth performance, gut health, meat quality, and fatty acid profile of Japanese quail.

In poultry production, the search for alternatives to in-feed antibiotics continues unabated. This study investigated the effects of dietary supplementation of black pepper and turmeric powder, separately or in combination, on the growth performance, gastrointestinal microbiota population, intestinal morphology, serum biochemical parameters, meat quality, and meat fatty acid profile in Japanese quails. Five hundred-day-old mixed-sex Japanese quail chicks were randomly assigned to one of five treatments: a control diet (CON); CON +0.2% antibiotic flavomycin as an antibiotic growth promoter (AGP); CON +0.5% turmeric powder (TUP); CON +0.5% black pepper powder (BPP); and CON +0.5% TUP, and 0.5% BPP (MIX). The findings showed that quail chicks fed AGP and TUP throughout the rearing period had better body weight gain (p = 0.007) and feed conversion ratio (p = 0.02) than the other treatments. The TUP, BPP, and MIX feeds reduced (p = 0.005) abdominal fat percentage. The MIX group had a better breast muscle water-holding capacity (p = 0.04) and lightness index (p = 0.02) and lower (p = 0.02) malondialdehyde concentration after 7 days of refrigerated storage. Feeding BPP, TUP, and MIX diets decreased (p = 0.001) serum cholesterol concentration. Quail chicks fed the CON diet showed significantly higher coliform counts in the crop and ileum (p < 0.001), whereas the lactic acid bacterial population was lower (p = 0.008) in the ileum. Birds that received the MIX diet exhibited a higher (p = 0.02) villus height to crypt depth ratio in the duodenum compared to the other groups. The tested feed additives increased (p < 0.001) villus height in the jejunum and ileum compared to other groups. Feeding the TUP, BPP, and MIX diets reduced (p < 0.001) total saturated fatty acid content and increased (p = 0.004) total polyunsaturated fatty acid concentration, where the MIX diet had the best results. Overall, the present data indicate that supplementing the basal diet with turmeric powder enhances the growth performance of Japanese quails. In some respects, such as gut health and meat quality, combining turmeric powder and black pepper powder was more effective than using them independently.

Productive parameters, cecal microflora, nutrient digestibility, antioxidant status, and thigh muscle fatty acid profile in broiler chickens fed with Eucalyptus globulus essential oil.

This study was conducted to evaluate the effects of different dietary inclusion of eucalyptus essential oil (EEO) on growth performance, relative organ weight, cecal microflora, nutrient digestibility, serum biochemical parameters, and thigh muscle fatty acid profile in broiler chickens. A total of six hundred 1-day-old male broiler chickens were randomly allocated into 5 treatment groups with 8 replicate pens, and each pen contained 15 birds. The experiment lasted for 42 d. Dietary treatments included corn-soybean meal-based diet supplemented with 0, 250, 500, 750, and 1,000 mg/kg EEO. The results indicated that dietary treatments had no effect on growth performance parameters in the 1 to 10 d period. From day 11 to 24, dietary supplementation of EEO showed a linear decrease in feed conversion ratio (FCR, P < 0.05). From day 25 to 42 and the overall period (1-42 d), broilers fed with different levels of EEO showed a linear increase in body weight gain (BWG) and reduction in feed conversion ratio (linear, P < 0.05). The relative organ weight were unaffected by any of the dietary treatments. With increasing levels of EEO, the cecal Escherichia coli (linear, P = 0.085) count showed a trend in reduction, and the cecal lactic acid bacteria population tended to increase (linear, P = 0.063). The apparent ileal digestibility of ether extract and organic matter were linearly and quadratically increased in response to increasing dietary EEO supplementation (P < 0.05). A trend of linear decrease in total cholesterol in the serum of birds fed with different levels of EEO was recorded (P = 0.074). Eucalyptus essential oil's inclusion increased serum superoxide dismutase linearly but reduced serum malondialdehyde linearly (P < 0.05). Dietary supplementation of EEO affected the fatty acid profile of thigh muscle so that increased the concentrations of total polyunsaturated fatty acids (linear, P < 0.05) and reduced total saturated fatty acid contents (linear, P < 0.05). Taken together, the inclusion of EEO increased BWG and decreased FCR during day 25 to 42 and day 1 to 42, and partially improved cecal microflora balance, nutrient digestibility, antioxidant activity, and thigh muscle fatty acid profile in broiler chickens.

Synergistic effects of fermented soybean meal and mannan-oligosaccharide on growth performance, digestive functions, and hepatic gene expression in broiler chickens.

This study investigated the effects of fermented soybean meal (FSBM) with or without mannan-oligosaccharide (MOS) prebiotic on growth performance, digestive functions, and hepatic IGF-1 gene expression of broiler chicken. A total of 480 day-old male broiler chickens were fed with 4 experimental diets for 6 wk. Experimental diets included corn-soybean meal diet (CON); corn-soybean meal diet + MOS prebiotic [0.2%, ActiveMOS; Biorigin, Brazile]; corn-FSBM diet [soybean meal (SBM) was totally replaced by FSBM]; and corn-FSBM + MOS prebiotic (MIX). Replacing dietary SBM with FSBM with or without MOS improved body weight gain and feed efficiency for the total grow-out period. However, the addition of MOS to the FSBM diet exhibited a greater body weight gain than other experimental diets. Villus height and villus height to crypt depth of the duodenum and jejunum were increased by feeding FSBM, MOS, and MIX diets. The ileal crude protein and energy digestibilities, as well as the activities of intestinal amylase and protease, and pancreatic protease, were improved by replacing SBM with FSBM, with or without MOS. The concentration of plasma 3-methylhistidine was reduced by FSBM and MOS, and synergistically by their combination. The MOS and FSBM diets upregulated the hepatic IGF-1 gene expression. However, there was an evident synergistic effect of FSBM supplemented with MOS in the upregulation of the hepatic IGF-1 gene expression. The outcomes of the current study indicate the FSBM and MOS had the potential to improve growth performance, hepatic IGF-1 expression, and intestinal morphology of broilers. Overall, the fermented products could be considered as functional feed that exhibits probiotic effects and the synergistic effects of prebiotics added to the fermented feeds may further improve the growth performance and gut health and functionality in broiler chicken.

Fermented soybean meal ameliorates Salmonella Typhimurium infection in young broiler chickens.

The present study was designed to investigate the effectiveness of dietary fermented soybean meal (FSBM) in comparison with prebiotic (Xylooligosaccharide; XOS) and probiotic (Lactic acid bacteria-based probiotic; LAC) for prevention of Salmonella Typhimurium (ST) infection in young broiler chickens from 1 to 24 d. The in vitro study revealed that soybean meal (SBM) fermentation increased the number of lactic acid bacteria (LAB) and lactic acid content and inhibited the growth of enterobacteria such as coliforms in SBM. A total of 450 day-old Ross 308 broiler chicks were placed in 30 pens (15 birds/pen) and allocated to 5 experimental treatments that consisted an un-supplemented basal diet and not infected (NC) or infected with ST (IC); IC plus 2 g XOS/kg; IC plus 0.2 g LAC/kg; and IC containing a complete replacement of SBM with FSBM. All birds (except NC) were orally administered with 0.5 mL of the ST solution (1 × 106 CFU/mL) at d 3 post-hatch. The ST challenge decreased body weight gain and feed intake (P < 0.05). The impairment of feed conversion ratio was alleviated by the addition of XOS, LAC, and FSBM in broiler diets compared with IC birds (P < 0.05). The ST infection reduced duodenum and jejunum villus height and increased Salmonella colonization throughout the gut as well as internal organ invasion compared with NC birds (P < 0.05). However, ST-infected broilers fed the XOS, LAC, and FSBM-containing diets showed a significant decrease in gut Salmonella colonization, and internal organ invasion, an increase in LAB counts, and improvement in intestinal mucosa morphology (P < 0.05). The tested feed additives or FSBM reduced heterophil to lymphocyte ratio compared with the IC group (P < 0.05). The results suggest that XOS, LAC, and FSBM improve growth performance, lower Salmonella colonization, and improve intestinal characteristics and immune response in ST-challenged broiler chicks. Therefore, fermented feeds due to having functional ingredients can be considered as an effective strategy to lessen the colonization of gut pathogens in broiler chickens.

Inclusion of fish waste silage in broiler diets affects gut microflora, cecal short-chain fatty acids, digestive enzyme activity, nutrient digestibility, and excreta gas emission.

A study was conducted to determine the impacts of dietary inclusion of fish waste silage (FWS) substituting soybean meal (SBM) on the performance, gut microflora, cecal short-chain fatty acid, apparent ileal digestibility (AID), digestive enzyme activity, and excreta noxious gas emission in broiler chickens. A total of 720-day-old male broilers (Cobb 500) were randomly allocated to 3 dietary treatments with 12 replicates each accommodating 20 birds for 42 d. Birds received diets as follows: a corn-SBM-based diet (CON) and 2 diets that replaced SBM with FWS at 60 g/kg (FWS60) and 120 g/kg (FWS120). During the entire period, replacing SBM with FWS60 and FWS120 increased body weight gain and decreased feed conversion ratio (P < 0.05). The lowest pH values in the crop, proventriculus, duodenum, ileum, and ceca were observed in birds fed diets containing FWS60 and FWS120 (P < 0.05). Likewise, birds fed FWS60 and FWS120 had lower numbers of coliform and E. coli and higher Bifidobacterium and Lactobacillus count in the ceca than those fed CON (P < 0.05). Feeding FWS60 and FWS120 diets increased cecal butyrate and lactic acid contents (P < 0.05). Birds fed FWS120 diets had greater intestinal amylase and protease activity than birds fed CON (P < 0.05); however, no significant differences were recorded between the treatment groups for digestive enzymes activity in the pancreas. The use of both levels of FWS in broiler diet increased AID of crude protein and ether extract (P < 0.05). The lowest excreta ammonia concentration was recorded in birds fed FWS120 diet (P < 0.05). In conclusion, the inclusion of FWS in broiler diets could improve the performance by enhancing gut function, derived from the improved digestive enzyme activity and nutrient digestibility, as well as by elevating the population of beneficial bacteria and short-chain fatty acid contents. Therefore, the biological silage can be considered as a promising option for recycling and recovery of fish wastes and effectively be used in broiler diets.

Pulicaria gnaphalodes powder in broiler diets: consequences for performance, gut health, antioxidant enzyme activity, and fatty acid profile.

The search constantly continues to identify potential alternatives to the use of antimicrobial growth promoters (AGP) in broiler production. This trial was conducted with broiler chicks to investigate the effect of different levels of Pulicaria gnaphalodes powder (PGP) in comparison with AGP, and probiotic (PRO) on growth performance, gut microflora, intestinal morphology, antioxidant enzyme activity, and fatty acid profile of meat. Ross 308 male broiler chicks (n = 576) were randomly assigned into 6 dietary treatments with 8 replicate pens per treatment and 12 birds per pen. The dietary treatments consisted of a basal diet as control (CON, with no additive), CON + 0.1% PGP, CON + 0.2% PGP, CON + 0.3% PGP, CON + 0.1% probiotic mixture (PRO), and CON + 0.05% bacitracin methylene disalicylate (AGP). Higher body weight gain and lower feed conversion ratio were obtained in birds fed AGP and 0.3% PGP compared with those fed CON and 0.1% PGP during grower, finisher, and the entire study (P < 0.05). On day 42, birds on PRO, 0.2 and 0.3% PGP treatments had lower counts of Escherichia coli and higher lactobacillus spp. in ileum and cecal contents compared to the CON and 0.1% PGP (P < 0.05). Villus height and villus height to crypt depth ratio of the duodenum were increased (P < 0.05) in response to dietary AGP, PRO, and 0.3% PGP. The diets containing PRO and different levels of PGP increased superoxide dismutase and glutathione peroxidase activities and decreased malondialdehyde level in serum, liver, and thigh muscle (P < 0.05). Total polyunsaturated fatty acid and n-3 fatty acid of birds fed PRO and PGP diets were higher than birds in CON and AGP groups (P < 0.05). In summary, supplementation of PGP could be a potential alternative to AGP in broiler diets due to its combined positive impacts on performance, serum cholesterol, intestinal health, antioxidant activity, and fatty acid profile in meat. Such effects, however, need to be further verified under compromised health or a disease challenge condition.

Fermented soybean meal exhibits probiotic properties when included in Japanese quail diet in replacement of soybean meal.

This study was conducted to investigate and compare the effect of dietary probiotic mixture (PM) and organic acid (OA) mixture with fermented soybean meal (FSBM) on performance, crop, and ceca microbiota, small intestine morphology, and serum lipid profile in Japanese quails. A total of 800 day-old Japanese quails was randomly allotted to 5 treatments with 8 replicate pens of 20 birds each, for 35 days. The experimental diets consisted of a control corn-soybean meal diet and 4 test diets: 1) control diet + 0.1% PM; 2) control diet + 0.2% OA mixture; 3) control diet + the combination of both PM and OA; and 4) an additives-free diet in which the soybean meal in the control diet was replaced with FSBM. The results indicated that in starter and the entire rearing periods, FSBM, PM, and PM+OA diets had significantly lower FCR compared to control or OA diets (P < 0.05). Birds in the FSBM group gained higher weight than control and OA birds (P < 0.05; 1 to 35 d). At d 21 and 35, birds fed the control diet showed significantly lower numbers of lactic acid bacteria in the crop, while coliforms were higher in the cecal content compared to the other diets (P < 0.05). At d 21, the villus height and villus height to crypt depth ratio in the duodenum and jejunum of birds fed PM, PM+OA, and FSBM diets were greater than in other treatments (P < 0.05). The serum concentrations of cholesterol and low-density lipoprotein cholesterol of birds fed PM, PM+OA, and FSBM diets were significantly lower than birds in control and AO groups (P < 0.05). The results obtained herein suggest that FSBM exhibits probiotic properties and, when used in substitution of SBM in Japanese quail diet, can improve growth performance, balance of desirable gastrointestinal microbiota in crop and ceca, small intestinal morphology, and serum lipid profile-likewise, a probiotic supplement.

Effects of Pediococcus acidilactici, mannan-oligosaccharide, butyric acid and their combination on growth performance and intestinal health in young broiler chickens challenged with Salmonella Typhimurium.

This study compared the efficacy of Pediococcus acidilactici, mannan-oligosaccharide, butyric acid, and their combination on growth performance and intestinal health in broiler chickens challenged with S. Typhimurium. Ross 308 male broilers (n = 420) were randomly assigned to one of the 6 treatments, resulting in 5 replicate pens of 14 chicks per treatment. The treatments included a negative control [(NC), no additive, not challenged]; positive control [(PC), no additive, but challenged with S. Typhimurium at d 3 posthatch], and 4 groups whereby birds were challenged with S. Typhimurium at d 3 posthatch and fed diets supplemented with either probiotic [0.1 g/kg Pediococcus acidilactici (PA)], prebiotic [2 g/kg mannan-oligosaccharides (MOS)], organic acid [0.5 g/kg butyric acid (BA)], or a combination of the 3 additives (MA). The S. Typhimurium challenge decreased feed intake, body weight gain and increased feed conversion ratio and reduced jejunum villus height (VH) and VH to crypt depth (CD) ratio (P < 0.05). Birds on the MA treatment exhibited similar performance to birds on the NC treatment (P > 0.05) and had a lower population of Salmonella in the ceca compared with birds on the PC treatment, at d 14 and 21 post-challenge (P < 0.05). The lowest heterophil to lymphocyte ratio was observed in birds on the MA and NC treatments (P < 0.05). Birds fed diets supplemented with MA or PA had greater VH and VH: CD ratio than birds on the PC treatment at d 7, 14 and 21 d post-challenge (P < 0.05). Suppressed amylase and protease activity was observed as a result of the S. Typhimurium challenge; the enzyme levels were restored in birds fed the additive-supplemented diets, when compared to the birds on the PC treatment, particularly at d 21 post-challenge (P < 0.05). These results indicate that dietary supplementation with a combination of PA, BA, and MOS in broiler chickens could be used as an effective tool for controlling S. Typhimurium and promoting growth performance.

Effects of fermented cottonseed meal on the growth performance, gastrointestinal microflora population and small intestinal morphology in broiler chickens.

1. This experiment was conducted to evaluate the effects of replacing dietary cottonseed meal (CSM) or fermented cottonseed meal (FCSM) for soya bean meal (SBM) on growth performance, carcass characteristics, gastrointestinal microbial populations, and intestinal morphology of broiler chickens. 2. CSM was fermented with Bacillus subtilis, Aspergillus niger and A. oryzae for 7 d. A total of 300 one-d-old male Ross 308 broiler chickens were used in a 42-d experiment in which the birds were randomly allotted to one of 5 dietary treatments (containing 0%, 10% and 20% CSM or FCSM) in a completely randomised design. Birds were reared on litter floor and had free access to feed and water during the experiment. 3. Results indicated that the fermentation process significantly reduced crude fibre and free gossypol, while it increased crude protein content and lactic acid bacteria (LAB) count in CSM. 4. The use of FCSM instead of CSM significantly improved growth performance of broilers. The abdominal fat yield in treatments containing FCSM was significantly lower than in the other treatments. The increase in the population of LAB in the crop and decrease in the population of coliforms in the ileum of birds fed on diets containing FCSM were more significant than in other birds. Villi in the duodenum and jejunum of the birds fed on diets containing FCSM were significantly higher than for the other experimental groups. 5. The positive effects of diets containing FCSM on growth performance and intestinal health of broiler chickens showed that this processed source of protein can serve as an appropriate alternative for SBM in diets for broiler chickens.