RESUMO
Olea europaea L. is one of the most important fruit trees in Tunisia because of its content of many potentially bioactive compounds. The aim of this study was to evaluate the chemical composition, antibiofilm, antiradical and acethylcholinesterase inhibitory activities from four Tunisian cultivars of Olea europaea L., i.e. 'Chetoui', 'Meski', 'Oueslati' and 'Jarboui'. By means of standardized methods, total phenols were determined and some of them characterized by HPLC. The total phenols and flavonoids contents were found to be the highest in the leaves of Chetoui cultivar. The Chetoui cultivar exhibited an important antioxidant and anticholinesterasic activity and an important anti-biofilm activity against Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Candida albicans, Enterococcus faecalis and Escherichia coli, with percentages of inhibition comprised between 83 and 93% at 2xMIC values. Olive leaves extracts could be used in the control of bacterial biofilms in food and food-related environments.