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1.
Prev Nutr Food Sci ; 17(2): 152-7, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24471077

RESUMO

This study was performed to increase the availability of Sedum sarmentosum (Dolnamul) and to improve the nutraceutical value of rice cakes (sulgitteok). The contents of crude protein, mineral, dietary fiber, water holding capacity, and hardness significantly and directly increased with lyophilized sedum powder (SP). Pore ratio and expansion rate decreased in samples containing more than 10% SP compared to the control. In a sensory evaluation, a positive correlation was detected between overall acceptability and taste (R (2)=0.99, p<0.01), and color (R (2)=0.72, p<0.05). Total polyphenol contents of the SP-treated groups were significantly elevated, accompanied by an increase in radical scavenging ability estimated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Replacing 10% of the rice powder with SP efficiently improved the antioxidant and nutritional values of sulgitteok as well as its the sensory quality.

2.
Plant Cell Rep ; 24(6): 350-6, 2005 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-16136267

RESUMO

In watermelon, grafting of seedlings to rootstocks is necessary because watermelon roots are less viable than the rootstock. Moreover, commercially important watermelon varieties require disease-resistant rootstocks to reduce total watermelon yield losses due to infection with viruses such as cucumber green mottle mosaic virus (CGMMV). Therefore, we undertook to develop a CGMMV-resistant watermelon rootstock using a cDNA encoding the CGMMV coat protein gene (CGMMV-CP), and successfully transformed a watermelon rootstock named 'gongdae'. The transformation rate was as low as 0.1-0.3%, depending on the transformation method used (ordinary co-culture vs injection, respectively). However, watermelon transformation was reproducibly and reliably achieved using these two methods. Southern blot analysis confirmed that the CGMMV-CP gene was inserted into different locations in the genome either singly or multiple copies. Resistance testing against CGMMV showed that 10 plants among 140 T1 plants were resistant to CGMMV infection. This is the first report of the development by genetic engineering of watermelons resistant to CGMMV infection.


Assuntos
Citrullus/genética , Citrullus/virologia , Imunidade Inata/genética , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/virologia , Tobamovirus/genética , Agricultura/métodos , Proteínas do Capsídeo/genética , DNA Complementar/genética , Regulação da Expressão Gênica de Plantas/genética , Técnicas de Transferência de Genes , Doenças das Plantas/genética , Doenças das Plantas/virologia , Raízes de Plantas/genética , Raízes de Plantas/virologia , Transformação Genética/genética
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