Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 6 de 6
Filtrar
Mais filtros








Base de dados
Intervalo de ano de publicação
1.
Environ Sci Pollut Res Int ; 26(15): 15458-15466, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30941712

RESUMO

External feather rinses and homogenized whole-carcass tissue matrix from two hummingbird species found in California (Calypte anna and Archilochus alexandri) were analyzed for the presence of nine insecticides commonly used in urban settings. Using a liquid chromatography-high-resolution mass spectrometry (LC-HRMS) analytical method, samples were quantitatively tested for the following neonicotinoids: dinotefuran, nitenpyram, thiamethoxam, acetamiprid, thiacloprid, clothianidin, imidacloprid, and sulfoxaflor. This analytical method was also used to qualitatively screen for the presence of approximately 150 other pesticides, drugs, and natural products. Feather rinsates from both hummingbird species had detectable concentrations of carbamate and neonicotinoid classes of insecticides. Combined results of the rinsate and homogenized samples (n = 64 individual hummingbirds) showed that 44 individuals (68.75%) were positive for one to four target compounds. This study documented that hummingbirds found in California are exposed to insecticides. Furthermore, feather rinsates and carcass homogenates are matrices that can be used for assessing pesticide exposure in small bird species. The small body size of hummingbirds limits traditional sampling methods for tissues and whole blood to evaluate for pesticide exposure. Thus, utilization of this analytical method may facilitate future research on small-sized avian species, provide insight into pesticide exposure, and ultimately lead to improved conservation of hummingbirds.


Assuntos
Cromatografia Líquida/métodos , Inseticidas/análise , Espectrometria de Massas em Tandem/métodos , Animais , Aves , California , Plumas/química , Guanidinas/química , Neonicotinoides/química , Nitrocompostos/química , Piridinas/química , Compostos de Enxofre/química , Tiametoxam/química , Tiazinas/química , Tiazóis
2.
J Vet Diagn Invest ; 31(3): 399-407, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30853007

RESUMO

We developed and validated a liquid chromatography-high-resolution mass spectrometry (LC-HRMS) analytical method for quantitatively measuring pesticide concentrations in small-body avian tissue samples using homogenized 1-2-d-old chicken carcasses as the test matrix. We quantified the following key insecticides: sulfoxaflor (sulfoximine class) and the neonicotinoids dinotefuran, nitenpyram, thiamethoxam, acetamiprid, thiacloprid, clothianidin, and imidacloprid. We used fortified chick carcass samples to validate method accuracy (80-125% recoveries), precision (<20% relative standard deviation), and sensitivity (≤1.2 ppb) for these targeted analytes. This method also uses full-scan, high-resolution MS to screen for the presence of a wide variety of other xenobiotics in bird carcasses. The utility of our screening process was demonstrated by the detection of carbaryl in some samples. This sensitive LC-HRMS analytical method for insecticide detection in a matrix of homogenized carcass is ideal for evaluating small birds for insecticide exposure. This novel whole-carcass method may allow for research studies of small-bodied, free-ranging avian species, and could provide insight regarding their exposure to multiple classes of environmental contaminants.


Assuntos
Galinhas , Resíduos de Drogas/química , Inseticidas/química , Neonicotinoides/química , Xenobióticos/química , Animais , Cromatografia Líquida de Alta Pressão/métodos , Limite de Detecção , Espectrometria de Massas em Tandem
3.
Am J Physiol Cell Physiol ; 302(3): C505-17, 2012 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-22049209

RESUMO

Previous studies have provided evidence that, in the early hours of ischemic stroke, a luminal membrane blood-brain barrier (BBB) Na-K-Cl cotransporter (NKCC) participates in ischemia-induced cerebral edema formation. Inhibition of BBB NKCC activity by intravenous bumetanide significantly reduces edema and infarct in the rat permanent middle cerebral artery occlusion model of ischemic stroke. We demonstrated previously that the BBB cotransporter is stimulated by hypoxia, aglycemia, and AVP, factors present during cerebral ischemia. However, the underlying mechanisms have not been known. Ischemic conditions have been shown to activate p38 and JNK MAP kinases (MAPKs) in brain, and the p38 and JNK inhibitors SB-239063 and SP-600125, respectively, have been found to reduce brain damage following middle cerebral artery occlusion and subarachnoid hemorrhage, respectively. The present study was conducted to determine whether one or both of these MAPKs participates in ischemic factor stimulation of BBB NKCC activity. Cultured cerebral microvascular endothelial cell NKCC activity was evaluated as bumetanide-sensitive (86)Rb influx. Activities of p38 and JNK were assessed by Western blot and immunofluorescence methods using antibodies that detect total vs. phosphorylated (activated) p38 or JNK. We report that p38 and JNK are present in cultured cerebral microvascular endothelial cells and in BBB endothelial cells in situ and that hypoxia (7% O(2) and 2% O(2)), aglycemia, AVP, and O(2)-glucose deprivation (5- to 120-min exposures) all rapidly activate p38 and JNK in the cells. We also provide evidence that SB-239063 and SP-600125 reduce or abolish ischemic factor stimulation of BBB NKCC activity. These findings support the hypothesis that ischemic factor stimulation of the BBB NKCC involves activation of p38 and JNK MAPKs.


Assuntos
Isquemia Encefálica/metabolismo , Células Endoteliais/metabolismo , Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo , Simportadores de Cloreto de Sódio-Potássio/metabolismo , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo , Animais , Antracenos/farmacologia , Barreira Hematoencefálica/metabolismo , Barreira Hematoencefálica/fisiopatologia , Encéfalo/irrigação sanguínea , Bumetanida/farmacologia , Bovinos , Hipóxia Celular , Células Cultivadas , Células Endoteliais/citologia , Imidazóis/farmacologia , Infarto da Artéria Cerebral Média , Microcirculação , Microvasos , Pirimidinas/farmacologia , Rubídio/metabolismo , Transdução de Sinais , Acidente Vascular Cerebral
4.
Semin Reprod Med ; 27(3): 240-9, 2009 May.
Artigo em Inglês | MEDLINE | ID: mdl-19401955

RESUMO

Estrogens are a group of pleiotropic steroid hormones that exhibit diverse mechanisms of action in multiple physiologic systems. Over the past 30 years, biomedical science has begun to appreciate that endogenous estrogens and their receptors display important roles beyond the reproductive system. Our growing appreciation of novel, nonreproductive functions for estrogens has fundamentally contributed to our knowledge of their role in human health and disease. Recent findings from the Women's Health Initiative have caused clinicians and scientists to question whether estrogens are protective factors or risk factors. In light of the dichotomy between basic science and clinical studies, this review will attempt to reconcile differences between them. We will focus on studies from our laboratory and others highlighting the beneficial properties of the most abundant endogenous estrogen, 17beta-estradiol, using in vivo and in vitro models of cerebral ischemia and neuronal injury. These studies demonstrate that 17beta-estradiol powerfully protects the brain using multiple molecular mechanisms that promote: (1) decreased cell death, (2) increased neurogenesis, (3) an enhancement of neurotrophic support, and (4) the suppression of proinflammatory pathways.


Assuntos
Lesões Encefálicas/metabolismo , Transtornos Cerebrovasculares/metabolismo , Estradiol/metabolismo , Neurogênese , Animais , Apoptose , Lesões Encefálicas/patologia , Lesões Encefálicas/prevenção & controle , Transtornos Cerebrovasculares/patologia , Transtornos Cerebrovasculares/prevenção & controle , Encefalite/metabolismo , Encefalite/prevenção & controle , Estradiol/uso terapêutico , Feminino , Humanos , Masculino , Modelos Animais , Neurogênese/efeitos dos fármacos , Fármacos Neuroprotetores/uso terapêutico , Receptores de Estrogênio/metabolismo
5.
J Neurotrauma ; 23(6): 830-52, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16774470

RESUMO

The neuroprotective effects of 17 beta -estradiol have been shown in models of central nervous system injury, including ischemia, brain injury, and more recently, spinal cord injury (SCI). Recent epidemiological trends suggest that SCIs in elderly women are increasing; however, the effects of menopause on estrogen-mediated neuroprotection are poorly understood. The objective of this study was to evaluate the effects of 17beta-estradiol and reproductive aging on motor function, neuronal death, and white matter sparing after SCI of post- and pre-menopausal rats. Two-month-old or 1- year-old female rats were ovariectomized and implanted with a silastic capsule containing 180 microg/mL of 17beta-estradiol or vehicle. Complete crush SCI at T8-9 was performed 1 week later. Additional animals of each age group were left ovary-intact but were spinal cord injured. The Basso, Beattie, Bresnahan (BBB) locomotor test was performed. Spinal cords were collected on post-SCI days 1, 7, and 21, and processed for histological markers. Administration of 17beta-estradiol to ovariectomized rats improved recovery of hind-limb locomotion, increased white matter sparing, and decreased apoptosis in both the post- and pre-menopausal rats. Also, ovary-intact 1-year-old rats did worse than ovary-intact 2-month-old rats, suggesting that endogenous estrogen confers neuroprotection in young rats, which is lost in older animals. Taken together, these data suggest that estrogen is neuroprotective in SCI and that the loss of endogenous estrogen-mediated neuroprotective seen in older rats can be attenuated with exogenous administration of 17beta-estradiol.


Assuntos
Estradiol/uso terapêutico , Fármacos Neuroprotetores , Ovariectomia , Maturidade Sexual/fisiologia , Traumatismos da Medula Espinal/tratamento farmacológico , Animais , Benzoxazinas , Peso Corporal/efeitos dos fármacos , Contagem de Células , Ciclo Estral/fisiologia , Feminino , Fluoresceínas , Corantes Fluorescentes , Membro Posterior/fisiologia , Marcação In Situ das Extremidades Cortadas , Indóis , Atividade Motora/efeitos dos fármacos , Compostos Orgânicos , Oxazinas , Ratos , Micção/efeitos dos fármacos
6.
Biol Reprod ; 69(3): 771-9, 2003 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-12724279

RESUMO

Recent studies indicate that trichloroethylene (TCE) may be a male reproductive toxicant. It is metabolized by conjugation with glutathione and cytochrome p450-dependent oxidation. Reactive metabolites produced along both pathways are capable of forming protein adducts and are thought to be involved in TCE-induced liver and kidney damage. Similarly, in situ bioactivation of TCE and subsequent binding of metabolites may be one mechanism by which TCE acts as a reproductive toxicant. Cysteine-conjugate beta-lyase (beta-lyase) bioactivates the TCE metabolite dichlorovinyl cysteine (DCVC) to a reactive intermediate that is capable of binding cellular macromolecules. In the present study, Western blot analysis indicated that the soluble form of beta-lyase, but not the mitochondrial form, was present in the epididymis and efferent ducts. Both forms of beta-lyase were detected in the kidney. When rats were dosed with DCVC, no protein adducts were detected in the epididymis or efferent ducts, although adducts were present in the proximal tubule of the kidney. Trichloroethylene can also be metabolized and form protein adducts through a cytochrome p450-mediated pathway. Western blot analysis detected the presence of cytochrome p450 2E1 (CYP2E1) in the efferent ducts. Immunoreactive proteins were localized to efferent duct and corpus epididymis epithelia. Metabolism of TCE was demonstrated in vitro using microsomes prepared from untreated rats. Metabolism was inhibited 77% when efferent duct microsomes were preincubated with an antibody to CYP2E1. Dichloroacetyl adducts were detected in epididymal and efferent duct microsomes exposed in vitro to TCE. Results from the present study indicate that the cytochrome p450-dependent formation of reactive intermediates and the subsequent covalent binding of cellular proteins may be involved in the male reproductive toxicity of TCE.


Assuntos
Cisteína/análogos & derivados , Cisteína/metabolismo , Adutos de DNA/biossíntese , Epididimo/metabolismo , Liases/metabolismo , Solventes/metabolismo , Transaminases/metabolismo , Tricloroetileno/metabolismo , Ducto Deferente/enzimologia , Animais , Biotransformação , Cisteína/toxicidade , Citocromo P-450 CYP2E1/efeitos dos fármacos , Citocromo P-450 CYP2E1/metabolismo , Adutos de DNA/efeitos dos fármacos , Relação Dose-Resposta a Droga , Poluentes Ambientais/metabolismo , Poluentes Ambientais/toxicidade , Epididimo/efeitos dos fármacos , Epididimo/enzimologia , Imuno-Histoquímica , Liases/efeitos dos fármacos , Masculino , Ligação Proteica/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Solventes/toxicidade , Transaminases/efeitos dos fármacos , Tricloroetileno/toxicidade , Ducto Deferente/citologia , Ducto Deferente/efeitos dos fármacos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA