A visual knowledge map analysis of mine fire research based on CiteSpace.

Mine fire has always been a serious disaster in coal industry; many academic achievements have poured out in the past two decades for solving this problem. In this study, visual analysis was conducted to grasp the hotspots and development trend of mine fire research. Papers that published in 1999-2020 were retrieved as the data basis from Web of Science, and CiteSpace was used to carry out knowledge map analysis. The results shown that number of papers has increased steadily since 2005 and achieved explosive growth since 2014. Deng J is the first published author among many scholars. China, the USA, and Australia are active areas in mine fire research and China University of Mining and Technology ranks first in this field. The highest co-occurrence frequency keyword is "spontaneous combustion." International Journal of Coal Geology and Fuel provide guidance for mine fire research. Fire prevention technology, low carbon, ecology, and sustainable development are the hot research in recent years. The prevention and control of mine fire from combustion mechanism should be further strengthened.

Effect of Virus Inactivation by Heating on Routine Clinical Laboratory Indicators in Serum.

BACKGROUND: Highly infectious viruses such as SARS-CoV-2, MERS-CoV, and Ebola virus represent a threat to clinical laboratory workers. We aimed to investigate how virus inactivation by heating at 60°C for 1 hour affects routine clinical laboratory indicators. METHODS: Each collected serum sample was separated into two aliquots, and various indicators were measured in first aliquot after inactivation by heating at 60°C for 1 hour and in the second after room-temperature incubation for 1 hour. RESULTS: Serological test results for 36 indicators remained mostly unaffected by heat inactivation, with a mean estimated bias of < 10%. By contrast, the results for alanine transaminase, pseudocholinesterase, creatine kinase, lactate dehydrogenase, cardiac troponin I, and myoglobin were affected by heat inactivation, with the mean esti-mated bias here being > 20%, which was further increased in the case of the results for alkaline phosphatase, lipase, and creatine kinase isoenzyme MB. Immunological serological measurements showed good agreement according to Kappa consistency checks after heat inactivation of serum. The results for alanine transaminase, pseudocholinesterase, creatine kinase, lactate dehydrogenase, cardiac troponin I, and myoglobin were significantly correlated (r > 0.95) after heat inactivation, and after correction by using a regression equation, the results for the indicators still retained a clinical reference value. CONCLUSIONS: Inactivation by heating at 60°C for 1 hour exerts no marked effect on numerous routine biochemical and immunological indicators in serum, but the detection values for certain items are significantly decreased. Our method could serve as reference strategy for routine serological diagnostics in patients with suspected or confirmed infection with highly pathogenic viruses.

Influencing factors of coronary artery stenosis in patients with stable coronary heart disease and a correlation analysis.

OBJECTIVE: To explore the relationship of the size and concentration of low density lipoprotein (LDL) particles and high density lipoprotein (HDL) particles and the coronary stenotic degree of stable coronary artery disease. METHODS: Altogether 62 patients with coronary disease confirmed by coronary angiography treated in our hospital from March 2019 to March 2020 were selected as the observation group, and 62 healthy persons in the same period were chosen as the control group. The particle size of LDL and HDL protein complexes were measured and we then calculated the concentration ratio to explore the relationship between the two types of lipoprotein particles and the degree of coronary artery disease. The Gensini integral method and the lesion numbers were used to evaluate the coronary stenotic degree. RESULTS: In comparison with the control group, the mean diameter of the average LDL particle in the observation group was smaller, but the type B ratio and Gensini score were higher (P<0.05). In comparison with the control group, the observation group had a higher Sd-LDL concentration ratio, as well as concentration of small-particle HDL, percentage of concentration of small-particle HDL in the whole HDL concentration and Gensini score (P<0.05). In comparison with the single-vessel disease group, the multi-vessel disease group had a smaller LDL concentration, as well as smaller large-particle HDL concentration and percentage of large-particle HDL concentration in the whole HDL concentration, and SD-LDL concentration ratio, small-particle HDL concentration, and percentage of small-particle HDL concentration in the whole HDL concentration and Gensini points were considerably higher (P<0.05). The Gensini score in the observation group showed negative correlations with LDL particle size (r=-0.375, P<0.05), and positive correlations with the concentration of large-particle HDL (r=0.301, P<0.05). CONCLUSION: The size and concentration of LDL and HDL are significantly related to the coronary stenotic degree in SCAD disease, suggesting that they play a role in the coronary stenotic degree.

LncRNA PART1 alleviated myocardial ischemia/reperfusion injury via suppressing miR-503-5p/BIRC5 mediated mitochondrial apoptosis.

BACKGROUND: Long non-coding RNA (lncRNA) is crucial for heart development and for adult heart structural maintenance and function. Herein, we performed a study to explore the effect of lncRNA PART1 on myocardial ischemia-reperfusion (I/R) injury by targeting BIRC5 through miR-503-5p pathway. METHODS: I/R model was created in vivo and vitro. The level of gene and protein was detected by RT-PCR and western blot. The apoptosis level was assessed by TUNEL and flow cytometry. Cell viability was determined by MTT. Mitochondrial function was evaluated by ATP content, ROS production, GSH level, and mitochondrial membrane potential. Cardiac function was confirmed by echocardiography, TTC staining, and H&E staining. RESULTS: Here, we found that the expression of lncRNA PART1 was down-regulated in the I/R hearts and H/R cardiomyocytes. Forced expression of PART1 remitted cardiac I/RI and H/R cardiomyocyte injury. Silencing of PART1 aggravated apoptosis and mitochondrial damage in cardiomyocytes. We found that PART1 functioned as a competing endogenous RNA of miR-503-5p, which decreased the expression of miR-503-5p. We further established BIRC5 as a target of miR-503-5p. Furthermore, PART1 prevented apoptosis and improved mitochondrial function in myocardial I/RI by targeting miR-503-5p/BIRC5. CONCLUSIONS: In summary, PART1 protected mitochondrial function via miR-503-5p/BIRC5 pathway in MI/RI, which may provide the new theoretical basis for MI/RI treatment in the clinic.

Conformationally Restricted α, α Directly Linked BisBODIPYs as Highly Fluorescent Near-Infrared Absorbing Dyes.

A new family of α, α directly linked bisBODIPYs was developed through a MoCl5-mediated intramolecular oxidative reaction. Due to the coplanar structure of the two conformationally locked BODIPY units, these bisBODIPYs showed well-extended conjugations and gave strong near-infrared absorptions and emissions with maxima around 760 and 780 nm, respectively, with high fluorescence quantum yields of ≤0.84. These dyes were successfully applied for in vitro and in vivo fluorescence imaging by taking advantage of their beneficial photophysical properties.

Effect of atorvastatin treatment on circulating adiponectin: a meta-analysis of randomized controlled trials.

BACKGROUND: Influences of atorvastatin on atherosclerosis and glycemic metabolism may be related to its potential impact on circulating adiponectin, an adipocyte that exerts anti-inflammatory, ant-atherosclerotic, and anti-oxidative effects. However, results of previous randomized controlled trials (RCTs) were not consistent. We performed a meta-analysis of RCTs to systematic evaluate the influence of atorvastatin on circulating adiponectin. METHODS: Relevant studies were identified via search of electronic databases of PubMed, Embase, and Cochrane's Library. A random-effect model was applied to pool the results via incorporating the potential heterogeneity. Predefined meta-regression and subgroup analyses were used to evaluate the influences of study characteristics on the outcome. RESULTS: Fourteen datasets from ten RCTs including 931 patients were included. Pooled results showed that atorvastatin did not significantly affect circulating adiponectin as compared with controls (weighed mean difference = - 0.27 µg/mL, 95% confidence interval: - 0.89 to 0.35 µg/mL, p = 0.39). Results of univariate meta-regression analyses showed that study characteristics including number of patients, mean age, proportion of male patients, body mass index, dose of atorvastatin, or treatment duration did not significantly affect the outcome (p all > 0.05). Moreover, subgroup analyses showed that atorvastatin did not significantly affect circulating adiponectin in studies stratified according to these study characteristics (p all > 0.05). CONCLUSIONS: Atorvastatin treatment does not significantly affect circulating adiponectin. Influences of atorvastatin on atherosclerosis and glycemic metabolism are not likely to be mediated by modulation of circulating adiponectin.

Protective effect of diethylcarbamazine inhibits NF-κB activation in isoproterenol-induced acute myocardial infarction rat model through the PARP pathway.

The present study investigated the protective effect of diethylcarbamazine in inhibiting nuclear factor (NF)-κB activation in isoproterenol­induced acute myocardial infarction (AMI) rats through the poly ADP ribose polymerase (PARP) pathway. Male albino Wistar rats were injected subcutaneously with isoproterenol (100 mg/kg/day) for 2 days to induce an AMI model. Diethylcarbamazine (50 mg/kg) was administered by gavage for 12 days prior to the isoproterenol-induced AMI. It was noted that diethylcarbamazine significantly inhibited AMI­induced casein kinase and lactate dehydrogenase levels, and reduced the AMI­induced wet heart weight to body weight ratio in AMI rats. Diethylcarbamazine treatment significantly weakened reactive oxygen species production and reduced the levels of tumor necrosis factor (TNF)­α, interleukin­6 and NF­κB/p65 in AMI rats. Western blotting demonstrated that diethylcarbamazine significantly suppressed the AMI­induced inducible nitric oxide synthase (iNOS), transforming growth factor (TGF)­ß1, cyclooxygenase­2 (COX­2) and PARP protein expression in AMI rats. The results demonstrated that the protective effect of diethylcarbamazine inhibited isoproterenol­induced AMI through the suppression of inflammation, iNOS, TGF­ß1, COX­2 and the PARP pathway, and revealed the clinical potential of diethylcarbamazine for therapeutic and clinical applications.

NbHSWP11, a microsporidia Nosema bombycis protein, localizing in the spore wall and membranes, reduces spore adherence to host cell BME.

Microsporidia are obligate intracellular parasites, and a derivative of fungi, which harbor a rigid spore wall to resist adverse environmental pressures. The spore wall protein, which is thought to be the first and direct protein interacting with the host cell, may play a key role in the process of microsporidia infection. In this study, we report a protein, NbHSWP11, with a dnaJ domain. The protein also has 6 heparin-binding motifs which are known to interact with extracellular glycosaminoglycans. Syntenic analysis indicated that gene loci of Nbhswp11 are conserved and syntenic between Nosema bombycis and Nosema ceranae. Phylogenetic tree analysis showed that Nbhswp11 clusters with fungal dnaJ proteins and has 98% identity with an N. bombycis dnaJ protein. Nbhswp11 was transcribed throughout the entire life stages, and gradually increased during 1-7 days, in a silkworm that was infected by N. bombycis, as determined by reverse-transcription PCR (RT-PCR). The recombinant protein NbHSWP11 (rSWP11-HIS) was obtained and purified using gene cloning and prokaryotic expression. Western blotting analysis displayed NbHSWP11 expressed in the total mature spore proteins and spore coat proteins. Indirect immunofluorescence assay revealed NbHSWP11 located at the spore wall of mature spores and the spore coats. Furthermore, immune electron microscopy showed that NbHSWP11 localized in the cytoplasm of the sporont. Within the developmental process of N. bombycis, a portion of NbHSWP11 is targeted to the spore wall of sporoblasts and mature spores. However, most of NbHSWP11 distributes on the membraneous structures of the sporoblast and mature spore. In addition, using a host cell binding assay, native protein NbHSWP11 in the supernatant of total soluble mature spore proteins is shown to bind to the host cell BmE surface. Finally, an antibody blocking assay showed that purified rabbit antibody of NbHSWP11 inhibits spore adherence and decreases the adherence rate of spores by 20% compared to untreated spores. Collectively, the present results suggest that NbHSWP11 is involved in host cell adherence in vitro. Therefore NbHSWP11, which has a dnaJ domain, may modulate protein assembly, disassembly, and translocation in N. bombycis.