[Numerical Study on the Characteristics of Regional Transport of PM2.5 in Shandong Province During Spring in 2014].

In this paper, spatial and temporal distribution, transportation and deposition of PM2.5 in Shandong Province in Spring, 2014 were all analyzed by applying PSAT of CAMx model and we also developed a transport matrix of PM2.5 between different cities in Shandong. The results showed that ρ(PM2.5) presented obvious spatial distribution characteristics; ρ(PM2.5) was higher in the western part compared to that in peninsula and ρ(PM2.5) was mainly concentrated below 2 000 m in vertical direction. Simulated horizontal transport flux of PM2.5 was up to 110 µg.(m2.s)-1 and the total deposition amount of PM2.5 was 23. 05 x 10(4) t in Shandong during Spring, 2014. Analysis of regional contribution found that the pollutants mainly came from local districts and the average external transport contribution to the whole Shandong province was about 21. 08% ± 3. 83% while it was 40. 45% ± 5. 96% between different cities; the contribution rates of Jinjinji distrcit, background and boundary conditions gradually increased by 7. 56% and 6. 18% respectively as the altitude increased.

[Relationship between the gene polymorphism in fibroblast growth factor-10 and susceptibility to chronic obstructive pulmonary disease 220 cases].

OBJECTIVE: Fibroblast growth factor 10 (FGF 10) signaling pathway is crucial to lung development and epithelial reconstruction. The aim of this study was to investigate the relationship between gene polymorphisms in FGF 10 and susceptibility to chronic obstructive pulmonary disease (COPD) in a Han population of North China. METHODS: The subjects included 220 patients with COPD (COPD group) and 285 healthy controls (control group). The COPD patients, admitted to our hospital from June 2007 to May 2012 because of acute exacerbation, included 142 males and 78 females, aging from 43 to 93 years [mean (74 ± 10)]. The control group included 183 males and 102 females, aging from 44 to 97 years [mean (72 ± 9)]. According to results of lung function testing, patients with COPD were divided into mild (8 cases), moderate (62 cases), and severe (48 cases) and very severe groups (102 cases). The genotype frequencies of FGF 10 gene single nucleotide polymorphisms (rs10473352, rs16873956, rs2973644, rs1011814, rs10402070 and rs723166) were genotyped by RFLP PCR-restriction fragment length polymorphism method and micro-sequencing (SnaPshot) technology assay. Chi-square test was used to perform the Hardy-Weinberg equilibrium test. Unconditional logistic of regression model was used to calculate odds ratio (OR) and 95%CI. The 2 groups were compared using t-test. RESULTS: The rs2973644 locus AA, GA and GG genotype frequencies in the COPD group and the control group were 108/220, 92/220, 20/220 and 167/285, 104/285, 14/285, respectively; while the frequencies of allele A and G were 308/440, 132/440 and 438/570, 132/570, respectively. The rs10473352 locus TT, TC and CC genotype frequencies in the COPD group and the control group were 142/220, 72/220, 6/220 and 202/285, 82/285, 1/285, respectively, the differences being statistically significant (χ(2) value were 6.021-6.213, P < 0.05). The rs10473352 allele T and C frequencies were 356/440, 84/440 and 486/570, 84/570, respectively, the differences being not statistically significant (χ(2) = 3.395, P > 0.05). The rs1011814 locus TT, TC and CC frequencies in mild and moderate compared with severe and very severe COPD disease were 16/68, 39/68, 13/68 and 29/150, 67/150, 54/150, respectively; while its allele T, C frequencies were 71/136, 65/136 and 125/300, 175/300, respectively; the differences being statistically significant (χ(2) values were 6.287 and 4.200, all P < 0.05). The remaining 5 tSNP (rs10473352, rs16873956, rs2973644, rs10402070 and rs723166) genotype distribution and allele frequencies were not significantly different (OR value were 0.606-1.357, P > 0.05). CONCLUSIONS: FGF 10 gene SNP sites rs2973644 and rs10473352 polymorphisms may be associated with susceptibility to COPD in Han population of North China. The SNP in rs1011814 may be associated with severity of COPD.

[Bioinformatics prediction of egA31 recombinant antigen epitopes of Echinococcus granulosus].

Specific primers were designed and synthesized based on the reported EgA31 gene of Echinococcus granulosus (GenBank Accession No. AF067807). Total RNA was extracted from E. granulosus and its EgA31 gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR). The PCR product was purified and cloned into plasmid pUCM-T, then transformed into Escherichia coli DH5alpha. The recombinant plasmids were screened and identified by digestion with restriction enzyme and PCR amplification. The positive recombinant plasmid pUCM-T/EgA31 was confirmed by sequencing and homology comparison. Five parameters and methods were used to predict B-cell epitopes in amino acid sequence of EgA31. The amplified DNA fragment (636 bp) had an identity of 100% with the EgA31 gene sequence of E. granulosus. B-cell and T-cell epitopes of EgA31 were probably at or adjacent to 32-79, 79-95, 105-124 and 141-154 in its amino acid sequence.

[Cluster analysis in micrangium detection in malignant nasal and paranasal sinus tumor].

OBJECTIVE: To study the application of cluster analysis in micrangium detection in malignant nasal and paranasal sinus tumor. METHODS: Microvessel density (MVD) counting and cluster analysis were used to detect the micrangium in patients with malignant nasal and paranasal sinus tumor to assess the association between the malignancy and MVD. RESULTS: According to cluster analysis, the MVD counting could be clustered into two groups, and the MVD showed significant differences between the tumor tissues, adjacent normal tissue and the control group (P<0.01), a result consistent with that by analysis of variance of the MVD. CONCLUSION: Cluster analysis can be used in clustering of MVD counting in malignant nasal and paranasal sinus tumor to simplify MVD counting, and offers an important analytic method for micrangium analysis in tumors.

[Cloning and sequence analysis of the egG1Y162 gene of Echinococcus granulosus].

A pair of primers (egG1Y162) were designed according to the nucleotide sequence of Echinococcus multilocularis emY162 antigen gene. Using genomic DNA and cDNA from protoscoleces and adult worms of E. granulosus as templates, PCR was performed with the primers to obtain fragments of egG1Y162 gene. PUCm-T/egG1Y162 recombinant plasmids and PUCm-T/egY162 cDNA recombinant plasmids were constructed and identified by PCR, digestion with restriction enzyme and sequencing. The egG1Y162 antigen gene was amplified in protoscoleces and adult worms of E. granulosus. The size of the egG1Y162 gene was 1 648 bp and cDNA was 459 bp, and GenBank accession numbers were AB458258 and AB458259, respectively.

Genetic analysis of protoplast fusant Xhhh constructed for pharmaceutical wastewater treatment.

In order to analyse genetic relationships between functional strain Xhhh previously constructed through protoplast fusion for pharmaceutical wastewater treatment and its parents, random amplification polymorphic DNA (RAPD) and polymerase chain reaction (PCR) were used to investigate genetic similarities among the strains based on genome and functional genes analyses. A total of 739 clear and consistent bands were produced in the RAPD fingerprint analysis with 40 primers. The genetic similarity indices between Xhhh and parental strains PC (Phanerochaete chrysosporium), SC (Saccharomyces cerevisiae) and XZ (native bacterium Bacillus sp.) were 36.21%, 37.73% and 37.48%, respectively. With PCR amplification and DNA sequencing, Xhhh was found containing functional genes of mnp and lip from PC, FLO1 from SC and 16S rDNA fragments from XZ. Experimental results of genetic analyses were in accordance with Xhhh biochemical and phenotypic characteristics, and protoplast fusion technique is considered as a promising technique in environmental pollution control.