Q-switched 1064 nm Nd: YAG laser restores skin photoageing by activating autophagy by TGFß1 and ITGB1.

Excessive ultraviolet B ray (UVB) exposure to sunlight results in skin photoageing. Our previous research showed that a Q-switched 1064 nm Nd: YAG laser can alleviate skin barrier damage through miR-24-3p. However, the role of autophagy in the laser treatment of skin photoageing is still unclear. This study aims to investigate whether autophagy is involved in the mechanism of Q-switched 1064 nm Nd: YAG in the treatment of skin ageing. In vitro, primary human dermal fibroblast (HDF) cells were irradiated with different doses of UVB to establish a cell model of skin photoageing. In vivo, SKH-1 hairless mice were irradiated with UVB to establish a skin photoageing mouse model and irradiated with laser. The oxidative stress and autophagy levels were detected by western blot, immunofluorescence and flow cytometer. String was used to predict the interaction protein of TGF-ß1, and CO-IP and GST-pull down were used to detect the binding relationship between TGFß1 and ITGB1. In vitro, UVB irradiation reduced HDF cell viability, arrested cell cycle, induced cell senescence and oxidative stress compared with the control group. Laser treatment reversed cell viability, senescence and oxidative stress induced by UVB irradiation and activated autophagy. Autophagy agonists or inhibitors can enhance or attenuate the changes induced by laser treatment, respectively. In vivo, UVB irradiation caused hyperkeratosis, dermis destruction, collagen fibres reduction, increased cellular senescence and activation of oxidative stress in hairless mice. Laser treatment thinned the stratum corneum of skin tissue, increased collagen synthesis and autophagy in the dermis, and decreased the level of oxidative stress. Autophagy agonist rapamycin and autophagy inhibitor 3-methyladenine (3-MA) can enhance or attenuate the effects of laser treatment on the skin, respectively. Also, we identified a direct interaction between TGFB1 and ITGB1 and participated in laser irradiation-activated autophagy, thereby inhibiting UVB-mediated oxidative stress further reducing skin ageing. Q-switched 1064 nm Nd: YAG laser treatment inhibited UVB-induced oxidative stress and restored skin photoageing by activating autophagy, and TGFß1 and ITGB1 directly incorporated and participated in this process.

Effects of smoke-free government policy in Qingdao, China: Evidence from the path analysis.

Smoke-free government(SFG), as a key tobacco control measure, has been added in Healthy China 2030 blueprint and Qingdao started the establishment of the demonstrative SFG in 2020.This study examined the effects of SFG policy on smoking and smoke-free(SF) environment after establishing the demonstrative SFG. This cross-sectional survey selected participants by simple random sampling from party and government agencies in Qingdao (N = 3625) and the participants filled in questionnaires online from November 31 to December 15, 2020. We utilized AMOS to set up models to analyze the direct and indirect effects of SFG policy. The findings showed that knowledge of SFG policy was positively associated with SF environment(ß = 0.29, P<0.001) and negatively associated with smoking(ß = -0.14,P<0.001). Knowledge of SFG policy had indirect effects on SF environment through four channels: independent mediation of discouraging smoking and attitude towards SFG policy, as well as chain mediation of these two factors, and perception of tobacco hazards and discouraging smoking, with indirect effects accounting for 33.5% of the total effect. Knowledge of SFG policy had indirect effects on smoking reduction via SF environment and two chain mediation: SF environment and attitude towards SFG policy, perception of tobacco hazards and intention to quit smoking, with indirect effects accounting for 50.2% of the total effect. The results provided the evidence that SFG policy not only had positive effects on creating SF environment but also on reducing smoking. The efficient policy infiltration to individuals played a vital role in the establishment of SFG. Attitude towards SFG policy, discouraging smoking and SF environment were the potential mediators for SFG policy. Findings in this study added more evidence related to effect mechanism of SFG policy and had a positive influence on promoting the implementation SFG policies for China and other countries.

Recent advances in the role of Yes-associated protein in dermatosis.

BACKGROUND: Dermatosis is a general term for diseases of the skin and skin appendages including scleroderma, psoriasis, bullous disease, atopic dermatitis, basal cell carcinoma, squamous cell carcinoma, and melanoma. These diseases affect millions of individuals globally and are a serious public health concern. However, the pathogenesis of skin diseases is not fully understood, and treatments are not optimal. Yes-associated protein (YAP) is a transcriptional coactivator that plays a role in the regulation of gene transcription and signal transduction. AIMS: To study the role of Yes-associated protein in skin diseases. MATERIALS AND METHODS: The present review summarizes recent advances in our understanding of the role of YAP in skin diseases, current treatments that target YAP, and potential avenues for novel therapies. RESULTS: Abnormal YAP expression has been implicated in occurrence and development of dermatosis. YAP regulates the cell homeostasis, proliferation, differentiation, apoptosis, angiopoiesis, and epithelial-to-mesenchymal transition, among other processes. As well as, it serves as a potential target in many biological processes for treating dermatosis. CONCLUSIONS: The effects of YAP on the skin are complex and require multidimensional investigational approaches. YAP functions as an oncoprotein that can promote the occurrence and development of cancer, but there is currently limited information on the therapeutic potential of YAP inhibition for cancer treatment. Additional studies are also needed to clarify the role of YAP in the development and maturation of dermal fibroblasts; skin barrier function, homeostasis, aging, and melanin production; and dermatosis.

ATP supplementation suppresses UVB-induced photoaging in HaCaT cells via upregulation of expression of SIRT3 and SOD2.

BACKGROUND: Skin photoaging is the damage caused by excessive exposure to ultraviolet (UV) irradiation. We investigated the effect of adenosine triphosphate (ATP) supplementation on UVB-induced photoaging in HaCaT cells and its potential molecular mechanism. MATERIALS AND METHODS: The toxicity of ATP on HaCaT cells was examined by the MTT assay. The effects of ATP supplementation on the viability and apoptosis of HaCaT cells were determined by crystal-violet staining and flow cytometry, respectively. Cellular and mitochondrial ROS were stained using fluorescent dyes. Expression of Bax, B-cell lymphoma (Bcl)-2, sirtuin (SIRT)3, and superoxide dismutase (SOD)2 was measured via western blotting. RESULTS: ATP (1, 2 mM) exerted no toxic effect on the normal growth of HaCaT cells. UVB irradiation caused the apoptosis of HaCaT cells, and ATP supplementation inhibited the apoptosis induced by UVB significantly, as verified by expression of Bax and Bcl-2. UVB exposure resulted in accumulation of cellular and mitochondrial reactive oxygen species (ROS), but ATP supplementation suppressed these increases. Expression of SIRT3 and SOD2 was decreased upon exposure to UVB irradiation but, under ATP supplementation, expression of SIRT3 and SOD2 was reversed, which was consistent with the reduction in ROS level observed in ATP-treated HaCaT cells after exposure to UVB irradiation. CONCLUSIONS: ATP supplementation can suppress UVB irradiation-induced photoaging in HaCaT cells via upregulation of expression of SIRT3 and SOD2.

Factors associated with secondhand smoke exposure among non-smoking employees in the workplace: A cross-sectional study in Qingdao, China.

OBJECTIVE: This study was conducted to describe secondhand smoke (SHS) exposure among non-smoking employees in the workplace, and identify factors related to SHS exposure in Qingdao. METHODS: The study participants covered key non-smoking places stipulated in the "Qingdao City Smoking Control Regulations," which included three categories: restaurants, bars, and office buildings. Airborne nicotine concentration in the workplace and saliva cotinine concentration of employees were measured. The questionnaire included employees' demographic factors, smoke-free measures in the workplace, employers' tobacco hazard knowledge, and attitudes towards smoke-free policy. RESULTS: A total of 222 non-smoking employees and 46 non-smoking employers were included in the study. The median concentrations of airborne nicotine and salivary cotinine were 0.389 µg/m3 and 0.575 ng/mL, respectively. Educational status, average number of workplace smokers per day, exposure time to SHS in the workplace, and whether smoking and non-smoking areas were divided significantly related to airborne nicotine concentration. Age, educational status, exposure time to SHS in the workplace, tobacco control training and publicity, and whether the employers support the "Qingdao Tobacco Control Regulation" were significantly related to salivary cotinine concentration. CONCLUSIONS: Despite the implementation of the "Qingdao Smoking Control Regulations" in 2013, the workplace remains an important location for SHS exposure. Interventions such as raising workers' awareness of the risks associated with SHS exposure through health education and developing smoking prevention and cessation programs to reduce SHS exposure in the workplace are urgently needed.

Influence factors analysis of COVID-19 Prevention behavior of chinese Citizens: a path analysis based on the hypothetical model.

BACKGROUND: Under the outbreak of Coronavirus disease 2019 (COVID-19), a structural equation model was established to determine the causality of important factors that affect Chinese citizens' COVID-19 prevention behavior. METHODS: The survey in Qingdao covered several communities in 10 districts and used the method of cluster random sampling. The research instrument used in this study is a self-compiled Chinese version of the questionnaire. Of the 1215 questionnaires, 1188 were included in our analysis. We use the rank sum test, which is a non-parametric test, to test the influence of citizens'basic sociodemographic variables on prevention behavior, and the rank correlation test to analyze the influencing factors of prevention behavior. IBM AMOS 24.0 was used for path analysis, including estimating regression coefficients and evaluating the statistical fits of the structural model, to further explore the causal relationships between variables. RESULTS: The result showed that the score in the prevention behavior of all citizens is a median of 5 and a quartile spacing of 0.31. The final structural equation model showed that the external support for fighting the epidemic, the demand level of health information, the cognition of (COVID-19) and the negative emotions after the outbreak had direct effects on the COVID-19 prevention behavior, and that negative emotions and information needs served as mediating variables. CONCLUSIONS: The study provided a basis for relevant departments to further adopt epidemic prevention and control strategies.

AgNPs seed priming accelerated germination speed and altered nutritional profile of Chinese cabbage.

In this study, the performance of AgNPs-priming (20, 40, and 80 mg/L) on the seed germination, yield, and nutritional quality of Chinese cabbage were evaluated. We found that AgNPs-priming at 20 and 40 mg/L for 15 h significantly accelerated seed germination speed and seedling development. Cabbage seeds primed with different concentrations of AgNPs (0, 20, 40, and 80 mg/L) were then planted in a real soil and allowed to grow for 1 month in greenhouse. Results showed that AgNPs-priming at 40 mg/L significantly increased cabbage yield by 44.3%. Gas chromatography-mass spectrometry (GC-MS) combining with sparse partial least squares-discriminant analysis (sPLS-DA) reveals that AgNPs priming altered the metabolite profile of cabbage leaves in a dose-dependent manner, decreasing carbohydrates and increasing nitrogen related compounds. This indicates that the metabolic stimulation during germination stage can influence the entire life cycle of cabbage. The nutritional quality of cabbage edible leaves was evaluated by liquid chromatography with tandem mass spectrometry (LC-MS/MS) and inductively coupled plasma-mass spectrometry (ICP-MS). Results showed that AgNPs-priming at all tested concentrations significantly increased the content of essential amino acids for several folds in cabbage leaves, including alanine, aspartic acid, glutamine, glutamic acid, histidine, isoleucine, leucine, lysine, phenylalanine, proline, serine, threonine, tyrosine, and valine. Meanwhile, AgNPs-priming (40 mg/L) significantly increased iron (Fe) content by 23.8% in cabbage leaves. Ag did not bioaccumulate in edible tissues, indicating the bio-safety of AgNPs-priming. These results suggest that AgNPs-priming is a low-cost and eco-friendly approach to increase crop yield and nutritional quality.

The effect of health literacy on health status among residents in Qingdao, China: a path analysis.

BACKGROUND: Health literacy is a public health goal which can be used as an independent factor of health outcomes. This study aimed to assess the association between health literacy and health status, as well as the two mediating factors of behavior and self-efficacy among residents aged 15-69 years in Qingdao. METHODS: A cross-sectional survey was implemented among residents aged 15-69 years (N = 3793) in Qingdao, China. A combination of stratified cluster random and proportional probability sampling methods was used to select subjects for this study. Data were collected using "The Chinese Citizen Health Literacy Questionnaire (2019)". We proposed a hypothetical model for the relationship between sociodemographic characteristics, health literacy, self-efficacy, health behavior, and health status, and used path analysis to validate the hypothesis. RESULTS: The path analysis showed that higher education (ß = 0.293) and income (ß = 0.135) are positively and directly associated with greater health literacy, which was positively associated with health status (ß = 0.057). Health literacy is a direct influencing factor of health behavior (ß = 0.070) and self-efficacy (ß = 0.099). Health behavior (ß = 0.041) and self-efficacy (ß = 0.173) exerted a positive direct effect on health status. The model explained 14.1% of variance for health literacy, 3.8% for self-efficacy, 5.7% for health behavior, and 15.0% for health status. CONCLUSIONS: Health literacy was identified to be a critical factor in health status. The results emphasized that the dissemination of health knowledge, development of healthy behavior, and cultivation of self-efficacy should be jointly promoted to reinforce the level of health status among residents in future work.

The effect of Q-switched 1064-nm Nd: YAG laser on skin barrier and collagen synthesis via miR-663a to regulate TGFß1/smad3/p38MAPK pathway.

BACKGROUND: Our previous research found that Q-switched 1064-nm Nd: YAG laser (1064-QSNYL) induces skin collagen synthesis by activating TGFß1/Smad3/p38MAPKs pathway. Moreover, a lot of studies shown that MicroRNAs (miRNAs) contribute to regulate collagen synthesis and skin barrier. Therefore, we intend to explore the mechanism of 1064-QSNYL on collagen synthesis and skin barrier through miRNAs. METHODS: We predicted the upstream miRNAs of TGFß1 by bioinformatics databases, and verified them through dual-luciferase reporter genes and Western blotting. The expression of collagen, skin barrier-related protein K10 and filaggrin, TIMP-1, and MMP-2 were detected by RT-qPCR and Western blotting, respectively. Moreover, we detected moisture content, elasticity value, TEWL value, SOD vitality, and hydroxyproline content to evaluate skin barrier of mice. H&E staining to observe the change of dermis thickness and inflammation and infiltration of mice skin. RESULTS: The results shown that TGFß1 was target gene of miR-663a. Moreover, we found that 1064-QSNYL activated TGFß1/smad3/p38MAPK pathway by down-regulating the expression of miR-663a in HaCaT, HDF cells, and mice, thereby promoting expression of Collagen I, Collagen IV, TIMP-1, K10, and filaggrin and inhibiting MMP-2. Furthermore, 1064-QSNYL contributed to moisture content, elasticity, SOD vitality, and hydroxyproline content via miR-663a to activate TGFß1/smad3/p38MAPK pathway. CONCLUSIONS: In summary, this study found for the first time that 1064-QSNYL contributed to collagen synthesis and skin repair via miR-663a to regulate TGFß1/smad3/p38MAPK pathway, thereby achieving skin rejuvenation.

Predicting the mortality of smoking attributable to cancer in Qingdao, China: A time-series analysis.

Smoking is the leading preventable cause of death and disability from cancer in China. To provide a scientific basis for tobacco control strategies and measures, this study investigated cancer deaths attributed to smoking from 2005 to 2017 and predicted mortality trends from 2018 to 2020 in Qingdao. We used time series analysis to evaluate the number of deaths attributed to smoking among residents over 35 years old in Qingdao and predicted mortality trends. The number of cancer deaths attributed to smoking in Qingdao from 2005 to 2016 was between 170 and 407, showing an upward trend and a certain periodicity. The best model is the ARIMA (2,1,0)×(3,1,0)12, with the lowest BIC (6.640) and the highest stationary R2 (0.500). The predicted cancer deaths curve attributed to smoking in 2017 is consistent with the actual curve, with an average relative error of 5.74%. Applying this model to further predict the number of cancer deaths attributed to smoking in Qingdao from January 2018 to December 2020, the predicted results were 5,249, 5,423 and 6,048, respectively. The findings emphasized the need to further strengthen tobacco control measures to reduce the burden of disease caused by tobacco.

Effects of nano-enabled agricultural strategies on food quality: Current knowledge and future research needs.

It is becoming more feasible to use nano-enabled agricultural products such as nanofertilizers and nanopesticides to improve the efficiency of agrochemical delivery to crop plants. Experimental results have shown that nano-agrochemicals have great potential for reducing the environmental impact of traditional agrochemicals while simultaneously significantly increasing crop production. However, emerging data suggest that nano-enabled products are not only capable of increasing yield, but also result in alterations in crop quality. Variation in proteins, sugars, starch content, as well as in metallic essential elements have been reported. Verbi gratia, albumin, globulin, and prolamin have been significantly increased in rice exposed to CeO2 engineered nanoparticles (ENPs), while CeO2, CuO, and ZnO ENPs have increased Ca, Mg, and P in several crops. Conversely, reductions in Mo and Ni have been reported in cucumber and kidney beans exposed to CeO2 and ZnO engineered nanomaterials, respectively. However, reports on specific effects in human health due to the consumption of agricultural products obtained from plants exposed to nano-agrochemicals are still missing.

Comparation of the phytotoxicity between chemically and green synthesized silver nanoparticles.

Green synthesis of silver nanoparticles (Ag NPs) by using plants extracts has provided an eco-friendly alternation for industry and agriculture application. Here, we prepared Ag NPs by using the cucumber leaves and rice husk extracts, and further assessed the antimicrobial activity and phytotoxicity of green synthesized Ag NPs (g-Ag NPs) comparing with chemically synthesized Ag NPs (chem-Ag NPs). The chem-Ag NPs had strong antibacterial activity on the growth of Escherichia coli, while g-Ag NPs by rice husks (gr-Ag NPs) exhibited long-term antibacterial effects. In terms of phytotoxicity, the chem-Ag NPs induced over-generation of ROS and activated plant antioxidant defense systems, thus resulting in the upregulation of MDA and Zn contents and downregulation of antioxidant capacity, carotenoid, globulin and Mo contents. However, g-Ag NPs significantly promoted cucumber photosynthesis by increasing chlorophyll contents. Besides, the green synthesized Ag NPs by cucumber extracts (gc-Ag NPs) increased protein contents and gr-Ag NPs stimulated the upregulation of Mn and the downregulation of Al, which were all positive effects. Overall, compared with chem-Ag NPs, g-Ag NPs exhibited long-tern antimicrobial properties and attenuated toxicity to plants, which could be used as potential nanopesticide or nanoscale growth regulator in agriculture.

Response of soil microbial communities to engineered nanomaterials in presence of maize (Zea mays L.) plants.

With the intended application of engineered nanomaterials (ENMs) in agriculture, accurate assessment the effect of these ENMs on soil microbial communities is especially necessary. Here, maize plants were cultivated in soil amended by SiO2, TiO2, and Fe3O4 ENMs (100 mg kg-1 soil) for four weeks. The impact of ENMs on bacterial community structure of the rhizosphere soil was investigated by using high-throughput sequencing. In addition, metabolites of maize rhizosphere soil were quantified by gas chromatography-mass spectrometry (GC-MS) based metabolomics. We found that the disturbance of ENMs on soil microbes are in the follow of Fe3O4>TiO2>SiO2. Exposure of Fe3O4 ENMs significantly reduced the abundance of nitrogen-fixation related bacteria Bradyrhizobiaceae (from 2.94% to 2.40%) and iron-redox bacteria Sediminibacterium (from 2.15% to 2.07%). Additionally, Fe3O4 ENMs significantly increased populations of Nocardioides (from 1.63% to 1.77%), Chitinophaga sancti (from 1.12% to 2.08%), Pantoea (from 1.31% to 2.22%), Rhizobiumand (from 1.41% to 1.74%) and Burkholderia-Paraburkholderia (from 1.50% to 2.09%), which are associated with carbon cycling and plant growth promoting. This study provides a perspective on the response of rhizosphere microbial community and low molecular weight metabolites to ENMs exposure, providing a comprehensive understanding of the environmental risk of ENMs.

Impact of Qingdao's smoke-free legislation on hospitalizations and mortality from acute myocardial infarction and stroke: an interrupted time-series analysis.

BACKGROUND AND AIMS: More than 20 cities in China have enacted local smoke-free laws that prohibit smoking in public places. Only two of these cities have examined the health impact of the law. Enacted in 2013, Qingdao's smoke free-law was stricter than most other municipal smoke-free laws because it did not allow designated smoking rooms. This study aimed to estimate the impact of Qingdao's smoke-free legislation on acute myocardial infarction (AMI) and stroke. DESIGN, SETTING AND PARTICIPANTS: We used an interrupted time-series design adjusting for underlying secular trends, seasonal patterns and meteorological factors to estimate the impact of the smoke-free law on AMI and stroke events among permanent residents aged 35 years or older in Qingdao, China. The study period was from 1 January 2010 to 31 December 2015, with a post-ban follow-up of approximately 2.5 years. MEASUREMENT: Outcome measures were weekly numbers of hospitalizations and deaths due to AMI/stroke. FINDINGS: A total of 10 371 and 56 101 patients were hospitalized, with a principal discharge diagnosis of AMI and stroke, respectively; 32 196 AMI and 49 711 stroke deaths occurred during the study period. Following the smoke-free legislation, an incremental 20% [95% confidence interval (CI) = 14-26%] and 8% (95% CI = 3-13%) decrease per year was observed in AMI and stroke hospitalization rates, respectively. Neither the immediate nor gradual change in AMI nor stroke mortality rates associated with the law was statistically significant (P > 0.05). Post-hoc subgroup analyses indicated that statistically significant reductions in AMI hospitalizations were evident among both the 35-64 (18% per year, 95% CI = 12-27%) and 65-84 (20% per year, 95% CI = 12-27%) age groups. Statistically significant reductions in stroke hospitalization were only in the older subgroup (13% per year, 95% CI = 8-18%). CONCLUSIONS: The 2013 smoke-free legislation in Qingdao, China was associated with reduction in hospitalization from acute myocardial infarction and stroke among permanent residents aged 35 years or older. There was no statistically significant reduction in mortality from acute myocardial infarctions or stroke.

Monitoring canopy recovery in a subtropical forest following a huge ice storm using hemispherical photography.

We estimated canopy structure and transmitted radiation using hemispherical photography in four monitoring years (2008-2010, 2016) following the 2008 huge ice storm in a subtropical forest in south China, so as to assess changes in canopy biophysical parameters during forest recovery from natural disturbance. Significant decrease in canopy openness (CO), transmitted direct radiation (TransDir), and transmitted diffuse radiation (TransDif), as well as significant increase in leaf area index (LAI), were found in the disturbed forest stand in the subsequent years following the ice storm, indicating rapid canopy recovery. In contrast, these biophysical parameters of the undisturbed forest stand were quite stable during the monitoring years. The strength of relationships between CO and other canopy biophysical parameters decreased in the disturbed stand along the monitoring years. The disturbed stand had common slopes for the CO-TransDir and CO-TransDif models in the first two monitoring years, but different slopes for the CO-LAI model between the first and the subsequent monitoring years, while the undisturbed stand had common slopes for all the regression models in the first three monitoring years following the huge ice storm. These results showed that stronger correlations of LAI or TransDir with CO were characteristic of less complex canopies, such as those damaged by disturbance; the sensitivity of transmitted radiation in response to CO decreased with canopy recovery. Our findings demonstrated that forests with different canopy structure varied in biophysical parameters, which can be quantified by hemispherical photography.

Cancer mortality attributable to cigarette smoking in 2005, 2010 and 2015 in Qingdao, China.

INTRODUCTION: Cigarette smoking is among the most important public health concerns worldwide and the leading preventable cause of illness and death associated with cancer, chronic obstructive pulmonary disease (COPD) and cardiovascular disease (CVD). Although Qingdao, China implemented smoking control measures in 2007 and smoke-free legislation in 2013, smoking-attributable cancer mortality remains at a high level. The present study aimed to facilitate changes in policy-making, intervention implementation, monitoring and evaluation by estimating and comparing the burden of smoking-attributable cancers in Qingdao during 2005, 2010 and 2015. METHODS: This study used the disease list from the Global Burden of Disease (GBD) study to quantify the burden of smoking-related cancer. Sex and age-specific smoking-attributable mortality data were collected from the Qingdao Municipal Center for Disease Control and Prevention using an online reporting system. The population-attributable fractions (PAFs) of smoking and smoking-attributable cancer mortality in 2005, 2010 and 2015 were estimated using the smoking impact ratio (SIR) and relative risks (RRs) and by multiplying the smoking-attributable fraction by total cancer mortality, respectively. RESULTS: The numbers of smoking-attributable cancer deaths increased from 2484 in 2005 to 2999 in 2010 and 4148 in 2015, with corresponding PAFs of 26.41%, 25.76% and 29.13%, respectively. The PAFs were higher among men (vs. women) for all cancers except cervical cancer. In 2005, lung, liver, esophageal and stomach cancers were most frequently associated with smoking-associated cancer mortality, and lung cancer had the greatest PAF, followed by nasopharyngeal, oral and esophageal cancers. Similar patterns were observed in 2010 and 2015. In 2015, 1 in 3 and 1 in 5 cancer deaths in men and women, respectively, were attributable to smoking, and 95% of these deaths were associated with lung, liver, esophageal or stomach cancer. Over time, downward and upward trends in smoking-attributable cancer deaths were respectively observed among people younger than and older than 50 years. CONCLUSIONS: The smoking-attributable cancer burden in Qingdao remains considerable, despite the implementation of tobacco control and smoke-free measures. Tobacco control efforts should remain a major public health priority.

Topography-associated thermal gradient predicts warming effects on woody plant structural diversity in a subtropical forest.

Understanding global warming effects on forest ecosystems will help policy-makers and forest managers design forest management and biodiversity conservation strategies. We examined the change in woody plant structural diversity in response to topography-associated thermal gradients in a subtropical forest with diverse abundance patterns. We found that energy distribution in a warming trend across slopes had significant effects on woody plant structural diversity. Except for total basal area of the adult trees, plant structural diversity significantly decreased with the increase of heat load. Heat load is significantly and negatively correlated with number of stems, number of species, and the number of stems of the most abundant species (Nmax) for seedlings, saplings, and individuals of all sizes. For the adult trees, heat load is significantly and positively correlated with number of stems and Nmax, and negatively but not significantly with number of species, indicating that large trees may not be as sensitive as seedlings and saplings to warming. Partial correlation analysis, having controlled for elevation, strengthened those relations in most cases. Our results reveal that warming will increase community productivity by enhancing the growth of large trees, but decrease species diversity and inhibit the regeneration of tree seedlings and saplings.

Effects of elevated mean and extremely high temperatures on the physio-ecological characteristics of geographically distinctive populations of Cunninghamia lanceolata.

Conventional models for predicting species distribution under global warming scenarios often treat one species as a homogeneous whole. In the present study, we selected Cunninghamia lanceolata (C. lanceolata), a widely distributed species in China, to investigate the physio-ecological responses of five populations under different temperature regimes. The results demonstrate that increased mean temperatures induce increased growth performance among northern populations, which exhibited the greatest germination capacity and largest increase in the overlap between the growth curve and the monthly average temperature. However,tolerance of the southern population to extremely high temperatures was stronger than among the population from the northern region,shown by the best growth and the most stable photosynthetic system of the southern population under extremely high temperature. This result indicates that the growth advantage among northern populations due to increased mean temperatures may be weakened by lower tolerance to extremely high temperatures. This finding is antithetical to the predicted results. The theoretical coupling model constructed here illustrates that the difference in growth between populations at high and low latitudes and altitudes under global warming will decrease because of the frequent occurrence of extremely high temperatures.

Mortality and Burden of Disease Attributable to Cigarette Smoking in Qingdao, China.

In China, smoking is the leading preventable cause of deaths by a disease. Estimating the disease burden attributable to smoking contributes to an evaluation of the adverse impact of smoking. To aid in policy change and implementation, this study estimated the population-attributable fractions (PAFs) of smoking, the all-cause mortality and the loss of life expectancy attributable to smoking in 2014 of Qingdao. PAFs were calculated using the smoking impact ratio (SIR) or current smoking rate (P) and relative risk (RR). We determined the smoking-attributable mortality by multiplying the smoking-attributable fraction by the total mortality. This study used the method of an abridged life table to calculate the loss of life expectancy caused by smoking. Smoking caused about 8635 deaths (6883 males, 1752 females), and accounted for 16% of all deaths; 22% in males and 8% in females. The leading causes of deaths attributable to smoking were lung cancer (38%), ischemic heart disease (19%) and chronic obstructive pulmonary disease (COPD, 12%). The PAF for all causes was 22%; 30% in males and 10% in females. Tobacco use may cause a reduction of about 2.01 years of the loss of life expectancy; 3 years in males and 0.87 years in females. The findings highlight the need for taking effective measures to prevent initiation and induce cessation.

Effects of climate change on plant population growth rate and community composition change.

The impacts of climate change on forest community composition are still not well known. Although directional trends in climate change and community composition change were reported in recent years, further quantitative analyses are urgently needed. Previous studies focused on measuring population growth rates in a single time period, neglecting the development of the populations. Here we aimed to compose a method for calculating the community composition change, and to testify the impacts of climate change on community composition change within a relatively short period (several decades) based on long-term monitoring data from two plots-Dinghushan Biosphere Reserve, China (DBR) and Barro Colorado Island, Panama (BCI)-that are located in tropical and subtropical regions. We proposed a relatively more concise index, Slnλ, which refers to an overall population growth rate based on the dominant species in a community. The results indicated that the population growth rate of a majority of populations has decreased over the past few decades. This decrease was mainly caused by population development. The increasing temperature had a positive effect on population growth rates and community change rates. Our results promote understanding and explaining variations in population growth rates and community composition rates, and are helpful to predict population dynamics and population responses to climate change.