RESUMO
To explore the cause leading to the difference in incidence of gastric cancer between Linqu and Cangshan populations, Shandong Province,and to provide evidence for the possible mechanism of high incidence of gastric cancer in Linqu County,the distribution of T59G mutation in Lewis gene was screened between Linqu and Cangshan populations by PCR-sequencing.The frequency of individuals with T59G mutation was 34.5% in Linqu population and 31.6% in Cangshan population,respectively,with no significant difference, P>0.05, and OR is 1.14 (95% CI,0.59-2.19). This suggests that Linqu and Cangshan populations may share the same genetic background.T59G mutation of Lewis gene could not be used as a genetic marker for Linqu and Cangshan populations and is not relevant to the difference in incidence of gastric cancer between them.
RESUMO
To study the relation between host genetic background and the susceptibility to H.pylori associated gastric cancer, PCR-sequencing and PCR-RFLP were used to screen SECRETOR gene polymorphisms in 142 subjects including 69 cancer patients and 73 non-cancer individuals from high-risk area of gastric cancer in Shandong and 93 control individuals from Beijing. Results showed that the difference in se(w)/se(w) distribution between non-cancer individuals and Beijing population was significant (P<0.01,OR is 3.06,95% CI,1.28-7.30), but that between cancer patients and non-cancer individuals was not with significance. SE gene polymorphism was not relevant to H.pylori infection. We concluded that Shandong population from high-risk area of gastric cancer shared a high distribution of se(w)/se(w) genotype,which could be considered as one of the genetic markers.