RESUMO
Gastric cancer (GC) is one of the crucial causes of cancer-associated death worldwide. This study aimed to investigate the biological function of miR-1286 in GC progression in vitro, evaluate the clinical value of serum miR-1286 to screen GC patients and explore its relationship with helicobacter pylori (HP) infection and peritoneal metastasis in GC patients. Expression of miR-1286 was measured by RT-qPCR. Cell Counting Kit-8 assay was utilized for measuring GC cell proliferation ability. The migration and invasion abilities of GC cells were measured using Transwell assays. Serum samples were obtained from 108 GC patients, 62 gastritis cases and 62 healthy volunteers. The diagnostic performance of miR-1286 was assessed using ROC analysis, and the predictive value of miR-1286 for peritoneal metastasis onset was analyzed using logistic regression analysis. miR-1286 played as a tumor suppressor in GC progression by inhibiting GC cell proliferation, migration and invasion. In GC patients, significantly decreased miR-1286 was observed compared to gastritis and healthy controls, and had considerable diagnostic accuracy to distinguish GC from the controls. A significant association was found between miR-1286 expression and HP infection, peritoneal metastasis and TNM stage. Moreover, miR-1286 was lowly expressed in GC patients with peritoneal metastasis, and independently predicted the occurrence of peritoneal metastasis in GC. miR-1286 acts as a tumor suppressor and a biomarker in GC, and is closely associated with HP infection and peritoneal metastasis onset. The methods to regulate miR-1286 may be novel strategies to improve the treatment of GC.
Assuntos
Biomarcadores Tumorais , Gastrite , MicroRNAs , Neoplasias Gástricas , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/sangue , Biomarcadores Tumorais/metabolismo , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Detecção Precoce de Câncer , Gastrite/genética , Gastrite/microbiologia , Gastrite/metabolismo , Regulação Neoplásica da Expressão Gênica , Genes Supressores de Tumor , Infecções por Helicobacter , Helicobacter pylori , MicroRNAs/genética , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologiaRESUMO
We explored the potential of combining carcinoembryonic antigen (CEA) and salivary mRNAs for gastric cancer (GC) detection.This study included 2 phases of study: a biomarker discovery phase and an independent validation phase. In the discovery phase, we measured CEA levels in blood samples and expression level of messenger RNAs (SPINK7, PPL, SEMA4B, SMAD4) in saliva samples of 140 GC patients and 140 healthy controls. We evaluated the clinical performance of each biomarker and developed a predictive model using machine-learning algorithm to differentiate GC patients and healthy controls.Our biomarker panel successfully discriminated GC patients from healthy controls with both high sensitivity (0.94) and high specificity (0.91). We next applied our biomarker panel in the independent validation phase, in which we recruited a new patient cohort of 60 GC patients and 60 healthy controls. Using our biomarker panel, the GC patients were discriminated from healthy controls in the validation phase, with sensitivity of 0.92 and specificity of 0.87.A combination of blood CEA and salivary messenger RNA could be a promising approach to detect GC.
Assuntos
Antígeno Carcinoembrionário/metabolismo , RNA Mensageiro/metabolismo , Neoplasias Gástricas/diagnóstico , Biomarcadores Tumorais/metabolismo , Estudos de Coortes , Diagnóstico por Computador , Feminino , Humanos , Aprendizado de Máquina , Masculino , Pessoa de Meia-Idade , Plaquinas/metabolismo , Estudo de Prova de Conceito , Saliva/metabolismo , Semaforinas/metabolismo , Sensibilidade e Especificidade , Inibidores de Serinopeptidase do Tipo Kazal/metabolismo , Proteína Smad4/metabolismo , Neoplasias Gástricas/metabolismoRESUMO
Mixed evidence exists regarding the relationship between environmental risk perception and pro-environmental behavior. This study uses an existing online survey conducted by the Center of Ecological Civilization (CEC) of China University of Geosciences from December 2015 to March 2016 and examines how cultural bias influences environmental risk perception and behavior. We found that an individual's pro-environmental behavior is not only influenced by environmental risk perception, but also by his or her cultural worldviews. Built on culture theory (CT), our empirical results suggest that young Chinese people are more located in "high-group" culture, where egalitarian culture and hierarchical culture dominate. The higher scores of hierarchical and egalitarian cultures of Chinese youth, the more likely they are to protect the environment. Moreover, the relationship between cultural worldviews and pro-environmental behaviors are mediated by perceived environmental risks.