RESUMO
Porous hydroxyapatite (HAp)/chitosan-alginate composite scaffolds were prepared through in situ co-precipitation and freeze-drying for bone tissue engineering. The composite scaffolds were highly porous and interconnected with a pore size of around 50-220 µm at low concentrations of HAp. As the HAp content increased, the porosity of the scaffolds decreased from 84.98 to 74.54%. An MTT assay indicates that the obtained scaffolds have no cytotoxic effects on MG-63 cells, and that they have good biocompatibility. An implantation experiment in mouse skulls revealed that the composite scaffold provides a strong positive effect on bone formation in vivo in mice. Furthermore, that HAp/chitosan-alginate composite scaffold has been shown to be more effective for new bone generation than chitosan-alginate scaffold.
Assuntos
Alginatos/metabolismo , Materiais Biocompatíveis/farmacologia , Osso e Ossos/citologia , Quitosana/metabolismo , Durapatita/metabolismo , Engenharia Tecidual/métodos , Alicerces Teciduais , Animais , Materiais Biocompatíveis/química , Materiais Biocompatíveis/metabolismo , Materiais Biocompatíveis/toxicidade , Osso e Ossos/fisiologia , Linhagem Celular Tumoral , Liofilização , Ácido Glucurônico/metabolismo , Ácidos Hexurônicos/metabolismo , Humanos , Camundongos , Osteogênese/efeitos dos fármacos , PorosidadeRESUMO
The apatite was formed on the titanium plates with NaOH and heat treatments by biomimetic process. The influence of titanium surface microstructure on the apatite formation onto titanium substrate in SBF solution was investigated. After biomimetic process, nano-sized apatite layers were found on the Ti plates with NaOH and heat treatments. However, the morphologies of formed apatite on substrate had different shapes such as coated, load-like, and linked. The morphology of apatite formed by biomimetic process would be affected by alkaline treatment, and substrate morphology and phase.