Phosphorus and selenium compounding mitigates Cr stress in peanut seedlings by enhancing growth homeostasis and antioxidant properties.

Peanut is an economically important crop, but it is susceptible to Cr contamination. In this study, we used peanut as experimental material to investigate the effects of exogenous P, Se interacting with Cr on the nutrient growth and antioxidant system of peanut seedlings by simulating Cr (0 µM, 50 µM, and 100 µM) stress environment. The results showed that exogenous P, Se supply could mitigate irreversible damage to peanut seedlings by altering the distribution of Cr in roots and aboveground, changing root conformation, and repairing damaged cells to promote growth. When the Cr concentration is 100 µM, it exhibits the highest toxicity. Compared to the control group P and Se (0 MM), the treatment with simultaneous addition of P + Se (0.5 + 6.0) resulted in a significant increase in root length and root tip number by 248.7% and 127.4%, respectively. Additionally, there was a 46.9% increase in chlorophyll content, a 190.2% increase in total surface area of the seedlings, and a respective increase of 149.1% and 180.3% in soluble protein content in the shoot and roots. In addition, by restricting the absorption of Cr and reducing the synthesis of superoxide dismutase SOD (Superoxide dismutase), CAT (Catalase), POD (Peroxidase), and MDA (Malonaldehyde), it effectively alleviates the oxidative stress on the antioxidant system. Therefore, the exogenous addition of P (0.5 MM) and Se (6.0 MM) prevented the optimal concentration of chromium toxicity to peanuts. Our research provides strong evidence that the exogenous combination of P and Se reduces the risk of peanut poisoning by Cr, while also exploring the optimal concentration of exogenous P and Se under laboratory conditions, providing a basis for further field experiments.

CircAGFG1 Promotes Ovarian Cancer Progression Through the miR-409-3 p/ZEB1 Axis.

OBJECTIVES: Circular RNAs (circRNAs) serve a crucial regulatory role in ovarian cancer (OC). Circular RNA ArfGAP with FG repeats 1 (circAGFG1) has been shown to be involved in promoting the progression of several cancers, containing triple-negative breast cancer, esophageal cancer and colorectal cancer. However, the function of circAGFG1 in OC is unclear. METHODS: Quantitative real-time reverse transcription PCR (RT-qPCR) was conducted to determine the expression levels of circAGFG1 and miR-409-3p. The proliferation and metastasis of cells were determined by colony formation assays, EdU assays, transwell assays and wound healing assays. In addition, a dual-luciferase reporter assay was performed to validate the mechanism between circAGFG1, miR-409-3p, and ZEB1. RESULTS: Our data suggested that circAGFG1 was significantly overexpressed in OC tissues compared to normal ovarian epithelial tissues. Overexpression of circAGFG1 was correlated with intraperitoneal metastasis, tumor recurrence and advanced stage. Additionally, circAGFG1 overexpression revealed a poor prognosis in OC patients. Knockdown of circAGFG1 suppressed the proliferation, invasion and migration of OC cells. Mechanistically, circAGFG1 acted as a sponge of miR-409-3p to enhance the expression level of zinc finger E-box binding homeobox 1 (ZEB1), thereby conferring OC cell proliferation, invasion and migration. Importantly, re-expression of ZEB1 effectively reversed the effects of circAGFG1 knockdown on OC cells. CONCLUSIONS: In summary, our study indicated that circAGFG1 may act as a prognostic biomarker and potential therapeutic target for patients with OC.

Application of long non-coding RNA RBAT1 in improving diagnosis and prognosis of ovarian carcinoma.

Tumorigenesis of bladder cancer and retinoblastoma is correlated with long non-coding RNA (lncRNA) RBAT1. However, the role of RBAT1 in ovarian carcinoma (OC) is unclear. Thus, the study explored the role of RBAT1 in OC. This research enrolled patients with OC ( n = 68), irritable bowel disease (IBD, n = 68, females), digestive tract inflammation (DTI, n = 68, females), urinary tract infection (UTI, n = 68, females), endometriosis (EM, n = 68, females), and healthy controls (HCs, n = 68) to collect plasma sampled. OC and paired non-tumor tissues were collected from patients with OC. RBAT1 accumulation in all samples was analyzed using RT-qPCR. The role of plasma RBAT1 in OC diagnosis was examined using the ROC curves with OC patients as the true positive cases and other patients and HCs as the true negative cases. The role of RBAT1 in predicting the survival of OC patients was analyzed using the survival curve study. RBAT1 was overexpressed in both OC plasma and tissues. Plasma RBAT1 levels were correlated with RBAT1 levels in OC tissues but not in non-tumor tissues. Plasma RBAT1 could distinguish OC patients from other patients and HCs. Patients with high plasma RBAT1 levels had a shorter survival. RBAT1 is overexpressed in OC and might be applied to improve the diagnosis and prognosis of OC.