Anti-ageing mechanism of topical bioactive ingredient composition on skin based on network pharmacology.

OBJECTIVE: To elucidate the anti-ageing mechanism of the combination of eight ingredients on the skin from a multidimensional view of the skin. METHODS: The target pathway mechanisms of composition to delay skin ageing were investigated by a network pharmacology approach and experimentally validated at three levels: epidermal, dermal, and tissue. RESULTS: We identified 24 statistically significant skin ageing-related pathways, encompassing crucial processes such as epidermal barrier repair, dermal collagen and elastin production, inhibition of reactive oxygen species (ROS), as well as modulation of acetylcholine and acetylcholine receptor binding. Furthermore, our in vitro experimental findings exhibited the following outcomes: the composition promotes fibroblast proliferation and the expression of barrier-related genes in the epidermis; it also stimulated the expression of collagen I, collagen III, and elastic fibre while inhibiting ROS and ß-Gal levels in HDF cells within the dermis. Additionally, Spilanthol in the Acmella oleracea extract contained in the composition demonstrated neuro-relaxing activity in Zebrafish embryo, suggesting its potential as an anti-wrinkle ingredient at the hypodermis level. CONCLUSIONS: In vitro experiments validated the anti-ageing mechanism of composition at multiple skin levels. This framework can be extended to unravel the functional mechanisms of other clinically validated compositions, including traditional folk recipes utilized in cosmeceuticals.

OBJECTIF: Élucider le mécanisme anti­âge de la combinaison de huit ingrédients sur la peau d'un point de vue multidimensionnel. MÉTHODES: Les mécanismes des voies cibles de la composition pour retarder le vieillissement de la peau ont été étudiés par une approche de pharmacologie des réseaux et validés expérimentalement à trois niveaux : épidermique, dermique et tissulaire. RÉSULTATS: Nous avons identifié 24 voies statistiquement significatives liées au vieillissement de la peau, englobant des processus cruciaux tels que la réparation de la barrière épidermique, la production de collagène et d'élastine dermiques, l'inhibition des espèces réactives de l'oxygène (ROS), ainsi que la modulation de l'acétylcholine et de la liaison des récepteurs de l'acétylcholine. En outre, nos résultats expérimentaux in vitro ont montré les résultats suivants : la composition favorise la prolifération des fibroblastes et l'expression des gènes liés à la barrière dans l'épiderme ; elle stimule également l'expression du collagène I, du collagène III et de la fibre élastique tout en inhibant les niveaux de ROS et de ß­Gal dans les cellules HDF au sein du derme. En outre, le Spilanthol de l'extrait d'Acmella oleracea contenu dans la composition a démontré une activité neuro­relaxante dans l'embryon de poisson zèbre, suggérant son potentiel en tant qu'ingrédient anti­rides au niveau de l'hypoderme. CONCLUSIONS: Les expériences in vitro ont validé le mécanisme anti­âge de la composition à plusieurs niveaux de la peau. Ce cadre peut être étendu pour élucider les mécanismes fonctionnels d'autres compositions validées cliniquement, y compris les recettes populaires traditionnelles utilisées dans les produits cosméceutiques.

A Case Series of Children With 2019 Novel Coronavirus Infection: Clinical and Epidemiological Features.

We first described the 2019 novel coronavirus infection in 10 children occurring in areas other than Wuhan. The coronavirus diseases in children are usually mild and epidemiological exposure is a key clue to recognize pediatric case. Prolonged virus shedding is observed in respiratory tract and feces at the convalescent stage.

Correlation between of small dense low-density lipoprotein cholesterol with acute cerebral infarction and carotid atherosclerotic plaque stability.

BACKGROUND: Acute cerebral infarction (ACI) is seriously harmful to human health worldwide. However, at present, the risk of disease onset is still not accurately predicted for some people. METHODS: Five hundred and nineteen patients with ACI and 300 healthy controls were included in this study. We divided the patients into three groups according to the results of cervical artery contrast-enhanced ultrasound. Ninety-five patients were in the CAS without plaque group, 108 patients were in the stable plaque group, and 316 patients were in the unstable plaque group. TC, TG, HDL-C, LDL-C, and sdLDL-C were measured in all subjects. RESULTS: The level of small dense low-density lipoprotein cholesterol (sdLDL-C) in the ACI group was significantly higher than that in the control group (P < 0.001). Logistic regression analysis showed that sdLDL-C was an independent risk factor for ACI (OR = 1.067, 95% CI: 1.041-1.093, P < 0.001); serum sdLDL-C was significantly higher in the unstable plaque group than in the stable plaque group and plaque-free group (P < 0.05, P < 0.001); serum sdLDL-C was also higher in the stable plaque group than the plaque-free group (P < 0.001). Logistic regression analysis showed that sdLDL-C was an independent risk factor for unstable carotid plaques (OR = 1.053, 95% CI: 1.038-1.068, P < 0.001); Spearman correlation analysis showed that sdLDL-C test results were positively correlated with carotid plaque stability (r = 0.363, P < 0.001). CONCLUSION: Small dense low-density lipoprotein cholesterol is an independent risk factor for the onset of ACI and may be an early serum marker for this disease.

Tracheoesophageal Fistula Caused by Tracheostomy in a Patient with Myasthenia Gravis after a Myasthenic Crisis.

A 57-year-old woman with myasthenia gravis (MG), who had experienced a myasthenic crisis, complained of coughing while drinking. At first, this appeared to be a sequela of the myasthenic crisis. However, after further investigation, the problem was identified as a tracheoesophageal fistula, a complication of tracheostomy. Here, we describe this special case in the hope that we can improve diagnostic accuracy by providing a reminder for other physicians to consider the differences between MG and tracheoesophageal fistula. It is very important to pay more attention to such situations in clinical scenarios and administer the most appropriate treatment without delay.

Proteomic analysis of Corylus heterophylla fisch in Changbai Mountain by shotgun approach.

Total proteins of Changbai Mountain hazelnut were extracted and then enzymolysed in solution. The mixture of peptides was separated on chromatograph and identified by shotgun proteomics approach. The identified proteins were analyzed by bioinformatics, 303 proteins were identified, of which 237 proteins (78.2%) were mainly distributing in a range of 10-70 kDa and 85 proteins (28.1%) were around pI 5-6. Based on the biological process cellular component and molecular function, these proteins were classified by Gene Ontology, the results showed that 183 proteins (73.8%) had catalytic activity (e.g. oxidoreductase, kinase, peptidase, etc.), and 170 proteins (68.5%) had binding activity. The protein profile provided a valuable basis for further research of hazelnut proteins and opened up new research avenues related to the function of these proteins.

Radiological and clinicopathological features of pPNET.

PURPOSE: To analyze the radiological and clinicopathological features of peripheral primitive neuroectodermal tumor (pPNET). MATERIALS AND METHODS: We retrospectively reviewed the computed tomography (CT) and magnetic resonance (MR) imaging of 14 cases of pPNET confirmed immunohistochemically. Relevant clinical data was also obtained. RESULTS: Of 14 cases, there were 10 male and 4 female with a mean age of 28 years. The main symptoms were the aggravated pain of the lesion site and enlarged palpable masses. Fourteen lesions were located in the chest wall (n = 4), retroperitoneum (n = 3), pelvic cavity (n = 2), intraspinal area (n = 2), nasal cavity (n = 1), leg ( n= 1) and iliac bone (n = 1). Eleven lesions originating from the soft tissue mainly demonstrated large and infiltrative mass with (n = 4) or without (n = 7) bony invasion. Ten cases of them showed heterogeneous density with patchy, necrotic foci and moderate heterogeneous enhancement. Four of them with large cystic area contained irregular septations within them. Two intraspinal cases had extradural lesions, extending through the intervertebral foramen and formed paraspinal soft tissue masses with vertebral destruction. The case within iliac bone demonstrated extensive mixed (lytic/osteoblastic) lesion with large adjacent soft tissue mass. After surgery or therapy, 10 patients died of local recurrences or metastases. CONCLUSION: The diagnosis of pPNET should be suggested in young patient when imaging depicts a large, ill-defined, soft-tissue mass containing area of cystic degeneration with internal septations and extending along the neural route and nearby the middle of the trunk. The key for correct diagnosis is to inosculate imaging to clinical data.

Study on evaluating dose to fishes based on its anatomic model.

Here, an anatomic model of mullet is developed on sampling, dissection, and measurement on site. A Monte Carlo code is used to compute the energy-absorbed fraction in tissues and organs of the mullet, and dose rates are calculated. Some previous methods are selected for comparison. The results calculated by means of a newly developed anatomic model indicate that the dose rate to each tissue/organ is different, and dose rates to some tissues/organs are much larger than those calculated based on previous uniform models. This suggests that it is necessary to exploit an anatomic model if there are various concentration factors within the organism. Taking the organism as a whole, the anatomic model has smaller internal dose rates and middle external dose rates among these methods.