RESUMO
To investigate the possible causal relationship between intestinal microflora and fractures using Mendelian randomization (MR). A 2-sample MR study of gut microbiota and fractures was conducted using a weighted inverse variance analysis with tests for heterogeneity, horizontal pleiotropy, and sensitivity. A causal association between fracture risk and specific bacterial taxa was identified at various taxonomic levels: 2 (Bacteroidia, Pâ =â .0304; Deltaproteobacteria Pâ =â .0304) at the class level, 3 (Bacteroidales, Pâ =â .0428; Desulfovibrionales, Pâ =â .0428; Enterobacteriales, Pâ =â .0208) at the order level, 2 (FamilyXI, Pâ =â .0304; Enterobacteriaceae Pâ =â .0332) at the family level, and 1 (Alistipes, Pâ =â .0405) at the genus level. This study revealed a causal relationship between gut microflora and fracture risk, demonstrating that the effect of different flora taxa flora abundance on fracture risk differs. It provides a reference for further studies.
Assuntos
Fraturas Ósseas , Microbioma Gastrointestinal , Humanos , Análise da Randomização Mendeliana , Análise de Variância , Enterobacteriaceae , Fraturas Ósseas/epidemiologia , Estudo de Associação Genômica AmplaRESUMO
To investigate the causal relationship between metformin use and osteoporosis and different subtypes of osteoporosis using a 2-sample Mendelian randomization method. Data from genome-wide association studies were analyzed, with the exposure factor being metformin and the outcome variables being osteoporosis and different subtypes. Mendelian randomization was performed using Inverse Variance Weighted (IVW), MR-Egger, and weight median (WM) methods, and heterogeneity tests, horizontal multivariate analyses, and sensitivity analyses were performed. The IVW method analysis with metformin and osteoporosis showed Pâ =â 1.53E-04, OR (95%CI)â =â 1.81E-02 (2.27E-02-1.44E-01); the IVW method analysis with metformin and postmenopausal osteoporosis with pathologic fracture showed Pâ =â 2.22E-01, OR (95%CI)â =â 4.89E-02 (3. 83E-04-6.23Eâ +â 00); the IVW method using metformin with osteoporosis with pathological fracture showed that Pâ =â 2.14E-01, OR (95%CI)â =â 1.64Eâ +â 00(5.78E-02-6.44E-04); the IVW method using metformin with pharmacological osteoporosis with pathological fracture showed that Pâ =â 9. 83E- 01, OR (95%CI)â =â 1.11Eâ +â 00 (3.99E-05-3.11Eâ +â 04); IVW method of metformin use and pharmacological osteoporosis showed that Pâ =â 5.99E-01, OR (95%CI)â =â 2.27Eâ +â 01 (2.00E-04-2.57Eâ +â 06); there is a causal relationship between metformin use and osteoporosis, but there is no causal relationship between metformin use and postmenopausal osteoporosis with pathological fracture, osteoporosis with pathological fracture, pharmacological osteoporosis, and pharmacological osteoporosis with pathological fracture, and metformin use is a protective factor for osteoporosis.