[Diagnostic and prognostic value of specific immune response to Cytomegaloviruses in pregnant women with a history of habitual abortion]. / Diagnosticheskaia i prognosticheskaia tsennost' spetsificheskogo immunnogo otveta k virusu tsitomegalii u beremennykh zhenshchin s privychnym nevynashivaniem beremennosti.

The authors analyze the usefulness of specific serologic tests for anti-CMV antibodies of the IgG and IgM classes for the prediction of the course of pregnancy and choice of immunocorrective therapy of women suffering from habitual abortions. Detection of specific IgM antibodies to cytomegalovirus with the use of Abbott diagnostic system was found highly informative, for it permitted a differential diagnosis of the cause of habitual abortions and monitoring of the treatment efficacy. Enzyme immunoassay of TBH in blood serum samples of pregnant women helped monitor the course of pregnancy.

[The importance of determining trophoblastic beta 1-glycoprotein (TBG) for the early diagnosis and prognosis of placental insufficiency in pregnant women with habitual abortion]. / Znachenie opredeleniia TBG dlia rannei diagnostiki i prognozirovaniia platsentarnoi nedostatochnosti u beremennykh s privychnym nevynashivaniem.

Blood serum TBH levels were measured by enzyme immunoassay over the course of pregnancy in 40 women with normal pregnancy (controls) and 80 with a history of habitual abortions. Weekly measurements of TBH concentrations have revealed a significant reduction of its secretion over the entire pregnancy course in women with a history of habitual abortions vs. the controls (p less than 0.05; 0.01). A reduced TBH level starting from the first pregnancy trimester (4-7-fold reduction) and no manifest elevation of its concentration were the signs predicting the formation of primary placental insufficiency; the risk of its development was particularly high if these signs were associated with low placentation, detachment of the chorion, and recurring threatened abortions. A 10-fold and lower decrease of TBH level, its gradual or drastic reduction are the unfavorable signs preceding a spontaneous abortion. Therefore TBH measurements may be regarded as a screening test for the prediction and early diagnosis of developing placental insufficiency in pregnant women with a history of habitual abortions.

[Histochemical and clinical-diagnostic study of placental alpha 1-microglobulin using monoclonal antibodies]. / Gistokhimicheskoe i kliniko-diagnosticheskoe izuchenie platsentarnogo alpha 1-mikroglobulina s pomoshch'iu monoklonal'nykh antitel.

Five stable hybridomas against placental protein PAMG-1 were obtained. Monoclonal antibodies were not cross reactive with other placental proteins and human serum albumin. The content of PAMG-1 during a physiological pregnancy increased to 40 ng/ml. 23 women with repeated abortions had an increased content of PAMG-1.

[The functions of the kidneys and the basic mechanisms of their regulation in premature infants]. / Funktsii pochek i osnovnye mekhanizmy ikh reguliatsii u nedonoshennykh detei.

Kidney function and some mechanisms of its regulation were studied in 161 premature babies. The renin-angiotensin-aldosterone system was found to play a certain role in controlling the water-electrolyte balance in premature infants. The incidence of pronounced and latent renal failure in infants with posthypoxia lesions of the central nervous system (CNS) of varying degrees was demonstrated. The informative value of calculation parameters of partial kidney functions as the early diagnostic criteria in pronounced and latent renal failure is confirmed. A role that the revealed posthypoxia hypermyoglobinemia might possibly play in the pathogenesis of acute renal failure in premature infants is discussed.

[Immunosedimentation analysis of proteins]. / Immunosedimentatsionnyi analiz belkov.

After performing experiments with protein sedimentation the ultracentrifuge test-tube was attached to the inferior opening of the channel in the agar block with a vertical gradient of saccharose concentration. The liquid with protein zones was removed from the test-tube to the channel, forcing a 60% solution of saccharose beneath the lower layers in the test-tube. Horizontal electrophoresis was performed so that the protein zones penetrated the width of the channel walls. A piece of agar gel was cut out from the walls along the whole height of the channel. That piece was placed on the slide and embedded with a mixture of agar and antiserum. Immunoelectrophoresis was made according to Lowrell. The method permits obtaining for the first time continuous sedimentograms during immunodevelopment of the protein zones derived on ultracentrifugation.