RESUMO
Glioma cell cultures are used in basic researches of tumor processes, personalized medicine for selecting treatment regimens depending on individual characteristics of patients and pharmacology for assessing the effectiveness of chemotherapy. Suppression of glioma culture growth without reduction of malignancy grade is common. Drug cancellation may be followed by substitution of precursor cells by more malignant clones. Therefore, analysis of culture cell malignancy grade is important. In the future, intraoperative analysis of glioma cell malignancy grade can be used to select individual therapy. OBJECTIVE: We analyzed the relationship between expression of marker genes TUBB3, CD133, CDK4, CDK6, CIRBP, DR4, DR5, EGFR, FGFR, FSHR, GDNF, GFAP, L1CAM, LEF1, MAP2, MDM2, MELK, NANOG, NOTCH2, OCT4, OLIG2, PDGFRA, PDGFA, PDGFB and SOX2 and glioma cell malignancy grade, as well as created appropriate prognostic model. MATERIAL AND METHODS: We analyzed expression of 25 marker genes in 22 samples of human glioma cultures using quantitative real-time PCR. Statistical analysis was performed using the IBM SPSS Statistics 26.0 software. We used the Kolmogorov-Smirnov and Shapiro-Wilk tests to assess distribution normality. Nonparametric Jonckheere-Terpstra and Spearman tests were applied. RESULTS: We obtained a prognostic model for assessing the grade III and IV glioma cell malignancy based on expression of marker genes MDM2, MELK, SOX2, CDK4, DR5 and OCT4. Predictive accuracy was 83% (Akaike information criterion -55.125).
Assuntos
Glioma , Humanos , Prognóstico , Glioma/genética , Receptor Notch2/genética , Receptor Notch2/metabolismo , Expressão Gênica , Proteínas Proto-Oncogênicas c-mdm2/genética , Proteínas Proto-Oncogênicas c-mdm2/metabolismo , Proteínas Proto-Oncogênicas c-mdm2/uso terapêutico , Proteínas Serina-Treonina Quinases/genética , Proteínas Serina-Treonina Quinases/metabolismo , Proteínas Serina-Treonina Quinases/uso terapêutico , Quinase 4 Dependente de Ciclina/genética , Quinase 4 Dependente de Ciclina/metabolismo , Quinase 4 Dependente de Ciclina/uso terapêutico , Proteínas de Ligação a RNA/genética , Proteínas de Ligação a RNA/uso terapêutico , Fatores de Transcrição SOXB1/genética , Fatores de Transcrição SOXB1/metabolismoRESUMO
A DNA collection of 239 Moscow and 62 SPB citizens has been investigated by means of a biochip for genotyping of Y-chromosome haplogroup markers: M130 (C), M145 (DE), P257 (G), M69 (H), U179 (I), M304 (J), M185 (L), M231 (N), M175 (0), P224 (R), L146 (R1a) and M343 (R1b). Haplogroup frequency distribution in populations native to Moscow and Saint-Petersburg has been obtained. Three subsamples varying in duration of residence (one, two or three generations) were compared. Increasing of J, G, R1b frequencies may be related to immigration from Caucasia and other regions.
Assuntos
Cromossomos Humanos Y/genética , Genótipo , Haplótipos/genética , Etnicidade/genética , Marcadores Genéticos/genética , Humanos , Moscou , Federação RussaRESUMO
Biochip has been developed which allowed to determine the following Y-chromosome haplogroups: C, DE, G, H, I, J, L, N, O, R in a DNA sample. The following SNPs were choosen as haplogroup markers: M130, M145, P257, M69, U179, M304, M185, M231, M175, P224, correspondingly. The genotyping included two-round PCR with fluorescent label incorporation into PCR product followed by hybridization with immobilized probes on biochip. The analysis of fluorescent signal ratios in pairs of immobilized probes "wild-type probe"--"group specific probe" for each of choosen polymorphic markers showed high accuracy of Y-haplogroup genotyping using biochip. The reliability of genotyping was confirmed by direct sequencing.