Evaluating the efficacy of a consumer-centric method for ecological sampling: Using bonobo (Pan paniscus) feeding patterns as an instrument for tropical forest characterization.

Characteristics of food availability and distribution are key components of a species' ecology. Objective ecological surveying used in animal behavior research does not consider aspects of selection by the consumer and therefore may produce imprecise measures of availability. We propose a method to integrate ecological sampling of an animal's environment into existing behavioral data collection systems by using the consumer as the surveyor. Here, we evaluate the consumer-centric method (CCM) of assessing resource availability for its ability to measure food resource abundance, distribution, and dispersion. This method catalogs feeding locations observed during behavioral observation and uses aggregated data to characterize these ecological metrics. We evaluated the CCM relative to traditional vegetation plot surveying using accumulated feeding locations across 3 years visited by a tropical frugivore, the bonobo (Pan paniscus), and compared it with data derived from over 200 vegetation plots across their 50 km2+ home ranges. We demonstrate that food species abundance estimates derived from the CCM are comparable to those derived from traditional vegetation plot sampling in less than 2 years of data collection, and agreement improved when accounting for aspects of consumer selectivity in objective vegetation plot sampling (e.g., tree size minima). Density correlated between CCM and plot-derived estimates and was relatively insensitive to home range inclusion and other species characteristics, however, it was sensitive to sampling frequency. Agreement between the methods in relative distribution of resources performed better across species than expected by chance, although measures of dispersion correlated poorly. Once tested in other systems, the CCM may provide a robust measure of food availability for use in relative food availability indices and can be incorporated into existing observational data collection. The CCM has an advantage over traditional sampling methods as it incorporates sampling biases relevant to the consumer, thereby serving as a promising method for animal behavioral research.

Genetic diversity of wild and cultivated Coffea canephora in northeastern DR Congo and the implications for conservation.

PREMISE: Many cultivated coffee varieties descend from Coffea canephora, commonly known as Robusta coffee. The Congo Basin has a century-long history of Robusta coffee cultivation and breeding, and is hypothesized to be the region of origin of many of the cultivated Robusta varieties. Since little is known about the genetic composition of C. canephora in this region, we assessed the genetic diversity of wild and cultivated C. canephora shrubs in the Democratic Republic of the Congo. METHODS: Using 18 microsatellite markers, we studied the genetic composition of wild and backyard-grown C. canephora shrubs in the Tshopo and Ituri provinces and multiple accessions from the INERA Yangambi Coffee Collection. We assessed genetic clustering patterns, genetic diversity, and genetic differentiation between populations. RESULTS: Genetic differentiation was relatively strong between wild and cultivated C. canephora shrubs, and both gene pools harbored multiple unique alleles. Strong genetic differentiation was also observed between wild populations. The level of genetic diversity in wild populations was similar to that of the INERA Yangambi Coffee Collection, but local wild genotypes were mostly missing from that collection. Shrubs grown in the backyards were genetically similar to the breeding material from INERA Yangambi. CONCLUSIONS: Most C. canephora that is grown in local backyards originated from INERA breeding programs, while a few shrubs were obtained directly from surrounding forests. The INERA Yangambi Coffee Collection could benefit from an enrichment with local wild genotypes to increase the genetic resources available for breeding purposes and to support ex situ conservation.

Importance of subterranean fungi in the diet of bonobos in Kokolopori.

Nonstaple food is a food resource which sole consumption does not allow the maintenance of regular physiological functions, thus constituting a minor portion of an individual's diet. Many primates consume nonstaple food such as meat, insects, and fungi. Hypotheses on the dietary importance of nonstaple food include its role as fallback food and as source of specific nutrients. We tested these two hypotheses by investigating mycophagy (i.e., the consumption of fungi) in a population of wild bonobos in the Kokolopori Bonobo Reserve, DRC. Specifically, we examined the relationship between fungus consumption and various factors relevant to bonobo feeding ecology (i.e., fruit abundance and the consumption of other food types). Additionally, we measured the deviation from linear travel when bonobos searched for fungi to evaluate the nature of fungus consumption (e.g., opportunistic or targeted). Lastly, we examined the nutritional content of the major fungus species consumed (Hysterangium bonobo) to test whether this food item was potentially consumed as source of specific nutrients. We found that bonobos spent a higher proportion of their time feeding on fungi when fruit abundance was higher, indicating that fungi were not consumed as a fallback food. Moreover, bonobos deviated from linear travel when visiting fungus patches more than observed when visiting fruit patches, suggesting that they actively sought out fungi. Lastly, initial analyses suggest that H. bonobo samples contained high concentration of sodium. Collectively, these results suggest that subterranean fungi appear to be attractive food source to Kokolopori bonobos, and that mycophagy may serve to supplement nutrients, like sodium, in bonobo diet.