Investigation of the regulatory effects of water and nitrogen supply on nitrogen transport and distribution in wolfberry fields.

Resource-based water shortages, uncoordinated irrigation, and fertilization are prevalent challenges in agricultural production. The scientific selection of appropriate water and fertilizer management methods is important for improving the utilization efficiency of agricultural resources and alleviating agricultural non-point source pollution. This study focused on wolfberry and compared the effects of four irrigation levels [full irrigation (W0, 75%-85% θf), slight water deficit (W1, 65%-75% θf), moderate water deficit (W2, 55%-65% θf), and severe water deficit (W3, 45%-55% θf)] and four nitrogen application levels [no nitrogen application (N0, 0 kg·ha-1), low nitrogen application (N1, 150 kg·ha-1), medium nitrogen application (N2, 300 kg·ha-1), and high nitrogen application (N3, 450 kg·ha-1)] on soil nitrate nitrogen (NO3 --N) transport, plant nitrogen allocation, and soil nitrous oxide (N2O) emissions during the harvest period of wolfberry. And this study used CRITIC-entropy weights-TOPSIS model to evaluate 16 water and nitrogen regulation models comprehensively. The results revealed the following: (1) The NO3 --N content of the soil decreased with increasing horizontal distance from the wolfberry. It initially decreased, then increased, and finally decreased with an increase in soil depth. The average NO3 --N content in the 0-100 cm soil layer ranged from 3.95-13.29 mg·kg-1, indicating that W0 > W1, W2, W3, and N3 > N2 > N1 > N0. (2) The soil NO3 --N accumulation ranged from 64.45-215.27 kg·ha-1 under varying water and nitrogen levels, demonstrating a decreasing trend with increasing horizontal distance. The NO3 --N accumulation at each horizontal distance increased with increasing irrigation and nitrogen application. The NO3 --N accumulation of W0N3 treatment increased by 5.55%-57.60% compared with the other treatments. (3) The total nitrogen content and nitrogen uptake in all wolfberry organs were W1 > W0 > W2 > W3, and N2 > N3 > N1 > N0. The maximum total nitrogen content and nitrogen uptake in W1N2 treatment were 3.25% and 27.82 kg·ha-1 in the roots, 3.30% and 57.19 kg·ha-1 in the stems, 3.91% and 11.88 kg·ha-1 in the leaves, and 2.42% and 63.56 kg·ha-1 in the fruits, respectively. (4) The emission flux and total emission of N2O increased with increasing irrigation and nitrogen application. The emission flux exhibited a transient peak (116.39-177.91 ug·m-2·h-1) after irrigation. The intensity of N2O emissions initially decreased and then increased with an increase in the irrigation amount. It also initially increased with increasing nitrogen application amount, then decreased, and finally increased again. The maximum emission intensity was observed under the W3N3 treatment (0.23 kg·kg-1). The N2O emission coefficients ranged from 0.17%-0.39%, in the order of W0 > W1 > W2 > W3 (except for N1) and N1 > N2 > N3. (5) Under varying water and nitrogen concentrations, N2O emission flux showed a positive linear correlation with soil pore water content and NO3 --N content and a negative linear correlation with soil temperature. The comprehensive evaluation revealed that a slight water deficit (65%-75% θf) combined with medium nitrogen application (300 kg·ha-1) decreased soil NO3 --N leaching, increased nitrogen uptake, and reduced N2O emission. These findings can serve as a reference for improving the efficiency and reducing emissions of wolfberry in the Yellow River irrigation region of Gansu Province and in similar climate zones.

Effects of Water and Nitrogen Control on the Growth Physiology, Yields, and Economic Benefits of Lycium barbarum Plants in a Lycium barbarum + Alfalfa System.

In the production of economic forests, there are common issues such as excessive application of water and fertilizer, redundant plant growth, and low economic benefits. Reasonable water and fertilizer management can not only help address these problems but also improve the absorption and use efficiency of water and fertilizer resources by plants, promoting the green and efficient development of the fruit and forestry industry. In order to explore a suitable water and nitrogen management mode for Lycium barbarum, field experiments were conducted in this study from 2021 to 2022. Specifically, four irrigation modes (according to the proportion ratio of soil moisture content to field moisture capacity θf, 45-55% θf (W1, severe water deficiency), 55-65% θf (W2, moderate water deficiency), 65-75% θf (W3, mild water deficiency), and 75-85% θf (W4, sufficient irrigation)) and four nitrogen application levels (0 kg·ha-1 (N0, no nitrogen application), 150 kg·ha-1 (N1, low nitrogen application level), 300 kg·ha-1 (N2, medium nitrogen application level), and 450 kg·ha-1 (N3, high nitrogen application level)) were set up to analyze the influences of water and nitrogen control on the plant height, stem diameter, chlorophyll content, photosynthetic characteristics and yield, and economic benefits of Lycium barbarum in the Lycium barbarum + Alfalfa system. The study results show that the plant height and stem diameter increment of Lycium barbarum increase with the irrigation amount, increasing first and then decreasing with the increase in the nitrogen application level. Meanwhile, the chlorophyll contents in Lycium barbarum continuously increase throughout their growth periods, with Lycium barbarum treated with W4N2 during all growth periods presenting the highest contents of chlorophyll. In a Lycium barbarum + Alfalfa system, the daily variation curve of the Lycium barbarum net photosynthetic rate presents a unimodal pattern, with maximum values of the daily average net photosynthetic rate and daily carboxylation rate appearing among W4N2-treated plants (19.56 µmol·m-2·s-1 and 157.06 mmol·m-2·s-1). Meanwhile, the transpiration rates of Lycium barbarum plants continuously decrease with the increased degree of water deficiency and decreased nitrogen application level. W1N2-treated plants exhibit the highest leaf daily average water use efficiency (3.31 µmol·s-1), presenting an increase of 0.50-10.47% in efficiency compared with plants under other treatments. The coupling of water and nitrogen has significantly improved the yields and economic benefits of Lycium barbarum plants, with W4N2-treated and W3N2-treated plants presenting the highest dried fruit yield (2623.07 kg·ha-1) and net income (50,700 CNY·ha-1), respectively. Furthermore, compared with other treatment methods, these two treatment methods (W4N2 and W3N2) exhibit increases of 4.04-84.08% and 3.89-123.35% in dried fruit yield and net income indexes, respectively. Regression analysis shows that, in a Lycium barbarum + Alfalfa system, both high yields and economic benefits of Lycium barbarum plants can be achieved using an irrigation amount of 4367.33-4415.07 m3·ha-1 and a nitrogen application level of 339.80-367.35 kg·ha-1. This study can provide a reference for improving the productivity of Lycium barbarum plants and achieving a rational supply of water and nitrogen in Lyciun barbarum + Alfalfa systems in the Yellow River Irrigation Area of Gansu, China, and other similar ecological areas.

The fairness of shared responsibility for embodied carbon emission: a case study of the Yellow River Basin, China.

Under the dual constraints of China's carbon peaking and carbon neutrality goals, as well as ecological protection and high-quality development of the Yellow River Basin, clarifying the embodied carbon emissions and responsibility sharing of inter-provincial trade is crucial to the carbon reduction strategy of the Yellow River Basin. This paper uses the MRIO (multi-regional input-output) model to measure the production-side and consumption-side responsibility sharing of nine provinces in the Yellow River Basin in 2012 and 2017, revealing the amount and direction of the embodied carbon transfer between provinces, and finally introduces the share of provincial value added as the responsibility sharing factor to compare and analyze the differences between the three responsibility sharing methods. The results show the following: (1) The embodied carbon emissions on the production side in most provinces of the Yellow River Basin were larger than that on the consumption side, with the most significant differences in Shanxi, Inner Mongolia, and Shandong, among which local demand carbon emissions and intermediate product transfer out of carbon emissions were the main causes of production-side carbon emissions. (2) In general, all provinces except Shaanxi were net carbon transfer-in regions, and the embodied carbon was mainly transferred to Beijing, Jiangsu, Guangdong, Zhejiang, and Hebei. (3) Shared responsibility for carbon emissions was jointly determined by the volume of embodied carbon trade and the ability to obtain value added, which lay between production and consumption side responsibility shares. (4) The Yellow River Basin had a large responsibility-sharing factor and embodied carbon trade, and thus needs to take more responsibility for emission reduction. This study is expected to provide scientific support for the strategy of differentiated emission reduction in the Yellow River Basin and enrich the regional carbon accounting methods.

Effects of irrigation and nitrogen application on soil water and nitrogen distribution and water-nitrogen utilization of wolfberry in the Yellow River Irrigation Region of Gansu Province, China.

To address the problems of extensive field management, low productivity, and inefficient water and fertilizer utilization in wolfberry (Lycium barbarum L.) production, an appropriate water and nitrogen regulation model was explored to promote the healthy and sustainable development of the wolfberry industry. Based on a field experiment conducted from 2021 to 2022, this study compared and analyzed the effects of four irrigation levels [75%-85% θf (W0, full irrigation), 65%-75% θf (W1, slight water deficit), 55%-65% θf (W2, moderate water deficit), and 45%-55% θf (W3, severe water deficit)] and four nitrogen application levels [0 kg·ha-1 (N0, no nitrogen application), 150 kg·ha-1 (N1, low nitrogen application), 300 kg·ha-1 (N2, medium nitrogen application), and 450 kg·ha-1 (N3, high nitrogen application)] on soil water distribution, soil nitrate nitrogen (NO3 --N) migration, yield, and water-nitrogen use efficiency of wolfberry. The soil moisture content of the 40-80 cm soil layer was higher than those of 0-40 cm and 80-120 cm soil layer. The average soil moisture content followed the order of W0 > W1 > W2 > W3 and N3 > N2 > N1 > N0. The NO3 --N content in the 0-80 cm soil layer was more sensitive to water and nitrogen regulation, and the cumulative amount of NO3 --N in the soil followed the order of W0 > W1> W2 > W3 and N3 > N2 > N1 > N0 during the vegetative growth period. There was no evidently change in soil NO3 --N accumulation between different treatments during the autumn fruit. The yield of wolfberry under the W1N2 treatment was the highest (2623.09 kg·ha-1), which was 18.04% higher than that under the W0N3 treatment. The average water consumption during each growth period of wolfberry was the highest during the full flowering period, followed by the vegetative growth and full fruit periods, and the lowest during the autumn fruit period. The water use efficiency reached a peak value of 6.83 kg·ha-1·mm-1 under the W1N2 treatment. The nitrogen uptake of fruit and nitrogen fertilizer recovery efficiency of fruit first increased and then decreased with increasing irrigation and nitrogen application. The treatment of W1N2 obtained the highest nitrogen uptake of fruit and nitrogen recovery efficiency of fruit, which were 63.56 kg·ha-1 and 8.17%, respectively. Regression analysis showed that the yield and water-nitrogen use efficiency of wolfberry improved when the irrigation amount ranged from 315.4 to 374.3 mm, combined with nitrogen application amounts of 300.0 to 308.3 kg·ha-1. Additionally, the soil NO3 --N residue was reduced, making it an optimal water and nitrogen management model for wolfberry planting. The present findings contribute novel insights into the production of wolfberry with saving water and reducing nitrogen, which helps to improve the level of wolfberry productivity in the Yellow River irrigation region of Gansu Province and other areas with similar climate.

The Association Between Hypoxia Improvement and Electroconvulsive Therapy for Major Depressive Disorder.

BACKGROUND: The occurrence of depression was related with a state of mild hypoxia for a long time. Hypoxia-inducible factor-2α (HIF-2α) modulates the process from acute to chronic hypoxia, consequently regulating changes in inducible nitric oxide synthase (iNOS). Increasing levels of iNOS combined with major depressive disorder (MDD) have been associated with the concentration of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), which increase the severity of depression. OBJECTIVE: The aim was to investigate whether depressive symptoms might be improved by regulating HIF-2α levels to decrease the degree of oxidative stress and inflammation using electroconvulsive therapy (ECT). METHODS: In this observational study, 49 MDD patients were divided into the ECT group (n=32) and control group (n=17). The Hamilton Depression Rating Scale (HAMD) was used to evaluate depressive symptoms of patients at enrollment and after 2 weeks of treatment. The levels of HIF-2α, NOS, IL-6, and TNF-α in plasma were analyzed accordingly. RESULTS: The total score in each dimension of HAMD decreased more efficiently in the ECT group than in the control group (p < 0.05). The plasma levels of IL-6 in the ECT group were notably decreased after the 2-week treatment (t = 3.596, p = 0.001). The decreased trend to statistical significance of HIF-2α was observed after treatment in the ECT group (p = 0.091). CONCLUSION: The present study demonstrated that the therapeutic effects of long-term ECT therapy for MDD may further benefit from and contribute to the improvement of MDD-associated chronic hypoxia.

DLG1-AS1 is activated by MYC and drives the proliferation and migration of hepatocellular carcinoma cells through miR-497-5p/SSRP1 axis.

BACKGROUND: Long non-coding RNAs (lncRNAs) have been reported to be biological regulators in hepatocellular carcinoma (HCC). DLG1 antisense RNA 1 (DLG1-AS1) has been found to be up-regulated in cervical cancer. However, its function and underlying mechanism in HCC remains unknown. METHODS: DLG1-AS1 expression was assessed in HCC cells and normal cell by RT-qPCR. Luciferase reporter assay, RNA pull down assay and RIP assay were used to demonstrate the interaction between DLG1-AS1 and miR-497-5p. RESULTS: DLG1-AS1 was highly expressed in HCC cells. Silencing of DLG1-AS1 led to the inhibition of HCC cell growth and migration. Besides, MYC induced the transcriptional activation of DLG1-AS1. MYC could facilitate HCC cellular processes by up-regulating DLG1-AS1. MiR-497-5p could interact with DLG1-AS1 in HCC cells. Down-regulation of miR-497-5p could reverse the impacts of DLG1-AS1 silencing on HCC cells. SSRP1 expression could be positively regulated by DLG1-AS1 but was negatively regulated by miR-497-5p. Knockdown of DLG1-AS1 suppressed tumor growth in nude mice. CONCLUSIONS: DLG1-AS1 is activated by MYC and functions as an oncogene in HCC via miR-497-5p/SSRP1 axis.